公开(公告)号：US20210078030A1

公开(公告)日：2021-03-18

申请号：US16927283

申请日：2020-07-13

Applicant: SHIMADZU CORPORATION

Inventor： Kazuteru TAKAHASHI ,  Kenta TERASHIMA

IPC: B05B15/522 ,  B05B15/555 ,  G01N1/31

Abstract: Provided is a sample pretreatment device configured to apply, to the surface of a sample, a solution in which a predetermined substance is dissolved or dispersed. In order to properly and efficiently unclog a nozzle due to the deposition of the crystal of a matrix substance, the device includes a spray unit (3) including a solution tube (32) for the solution to pass through, a gas tube (33) for a spray gas to pass through, and a nozzle part (30) configured to spray the solution arriving at the terminal end of the solution tube by ejection of the spray gas through the gas tube, as well as a cleaning liquid supplier (4, 41) configured to put a cleaning liquid on an opening of the nozzle part from outside the spray unit.

公开(公告)号：US20210278379A1

公开(公告)日：2021-09-09

申请号：US17186356

申请日：2021-02-26

Applicant: SHIMADZU CORPORATION

Inventor： Ryuta SHIBUTANI ,  Kazuteru TAKAHASHI

IPC: G01N30/72 ,  G01N30/30

Abstract: A combined analyzer includes a thermal analyzer, a trap, a gas chromatograph, a mass spectrometer, a first flow path to which a gas generated in the thermal analyzer is supplied, a second flow path that branches from the first flow path and is connected to the mass spectrometer, a third flow path that branches from the first flow path and is connected to the trap, a fourth flow path that connects the trap and a column included in the gas chromatograph, and a fifth flow path that connects the column and the mass spectrometer.

公开(公告)号：US20210101153A1

公开(公告)日：2021-04-08

申请号：US16990081

申请日：2020-08-11

Applicant: SHIMADZU CORPORATION

Inventor： Kenta TERASHIMA ,  Kazuteru TAKAHASHI

IPC: B01L3/00 ,  G01N1/38 ,  H01J49/16 ,  G01N27/62

Abstract: A first container for housing a reagent and a second container for housing the first container inside are provided. The pressurization mechanism pressurizes a pressurization space formed outside the first container in the second container to pressurize the inside of the first container communicating with the pressurization space. The reagent is drawn out from the pressurized inside of the first container through a pipe.

公开(公告)号：US20230221225A1

公开(公告)日：2023-07-13

申请号：US18013397

申请日：2021-03-09

Applicant: Shimadzu Corporation

Inventor： Kazuteru TAKAHASHI ,  Masahito UEDA ,  Hidefumi YAMAGATA ,  Shinsuke INOUE ,  Shinji HAMASAKI

IPC: G01N1/34 ,  G01N1/16

CPC classification number: G01N1/34 ,  G01N1/16

Abstract: A sample purification apparatus includes a container for separating, with a heavy solution, a mixed sample based on a specific gravity difference and a collector that collects a component in the mixed sample lighter in specific gravity than the heavy solution by receiving a supernatant flowed out from the container. The container includes a flow-out port provided in an uppermost portion of the container and a flow-out path that guides the supernatant flowed out through the flow-out port to the collector. the container has a horizontal cross-sectional area which gradually decreases upward starting from a prescribed height of the container to a height where the flow-out port is located.

公开(公告)号：US20160172174A1

公开(公告)日：2016-06-16

申请号：US14820622

申请日：2015-08-07

Applicant: SHIMADZU CORPORATION

Inventor： Kazuteru TAKAHASHI

IPC: H01J49/04 ,  B05D1/04 ,  B05B5/16

CPC classification number: B05B5/087 ,  B05B5/005 ,  B05B5/03 ,  B05B5/0535 ,  B05B5/16 ,  B05B5/1608 ,  B05C5/02 ,  B05C5/0208 ,  B05D1/04 ,  H01J49/0418 ,  H01J49/164

Abstract: A matrix film forming device including a first electrode plate including a mounting surface on which a sample plate P is mounted; a second electrode plate arranged facing said mounting surface; a nozzle which sprays a liquid containing a matrix substance by an electrospray process into the space between the first electrode plate and the second electrode plate and is arranged such that the first electrode plate and second electrode plate are not present over the central axis of the spray stream; and an electric field forming device which forms, between the first electrode plate and the second electrode plate, an electric field causes liquid drops containing charged matrix substance contained in the spray stream to move toward said mounting surface.

Abstract translation: 一种矩阵膜形成装置，包括：第一电极板，包括安装有样品板P的安装面; 布置成面向所述安装表面的第二电极板; 喷嘴，其通过电喷雾法将含有基质物质的液体喷射到第一电极板和第二电极板之间的空间中，并且布置成使得第一电极板和第二电极板不存在于喷雾的中心轴线上方 流; 以及在第一电极板和第二电极板之间形成电场的电场形成装置，使包含在喷射流中的含有基质物质的液滴朝向所述安装面移动。

公开(公告)号：US20130171349A1

公开(公告)日：2013-07-04

申请号：US13674745

申请日：2012-11-12

Applicant: SHIMADZU CORPORATION

Inventor： Kazuteru TAKAHASHI ,  Kiyoshi OGAWA

IPC: C23C16/52

CPC classification number: C23C16/52 ,  C23C14/12 ,  C23C14/24 ,  C23C14/54 ,  C23C14/547 ,  C23C14/548 ,  G01B11/0625

Abstract: To provide a sample preparation device that is appropriate for the formation of a matrix film for MALDI through vacuum vapor deposition. A sample preparation device is provided with: a sample substrate support unit 23 for supporting a substance to be analyzed on a substrate S so that the substance faces a vapor deposition source 21 for a matrix substance J; a light amount measurement unit for irradiating a matrix film vapor deposited on the substrate S with measurement light diagonally and detecting the amount of measurement light that has transmitted through or has been reflected from the above-described matrix film diagonally; and an adhesion prevention means 23a for preventing the matrix substance that has flown off from the above-described vapor deposition source 21 from adhering to the above-described light amount measurement unit.

Abstract translation: 提供适于通过真空气相沉积形成用于MALDI的基质膜的样品制备装置。 样品制备装置具有：样品基板支撑单元23，用于在基板S上支撑待分析物质，使得物质面向基质物质J的气相沉积源21; 光量测量单元，其用测量光对角地照射沉积在基板S上的基质膜蒸气并检测已经透射或已经从上述矩阵膜反射的测量光的量; 以及用于防止从上述蒸镀源21流出的基质物质粘附于上述光量测定单元的防附着装置23a。

公开(公告)号：US20230258544A1

公开(公告)日：2023-08-17

申请号：US18013379

申请日：2020-10-28

Applicant: Shimadzu Corporation

Inventor： Kazuteru TAKAHASHI ,  Masahito UEDA ,  Hidefumi YAMAGATA ,  Shinsuke INOUE ,  Shinji HAMASAKI

IPC: G01N1/34 ,  G01N35/10

CPC classification number: G01N1/34 ,  G01N35/1009

Abstract: A sample purification apparatus includes a container for separating a mixed sample based on a specific gravity difference with the use of a heavy solution, a heavy solution introduction portion for introduction of the heavy solution into the container, a flow-out portion provided vertically above the heavy solution introduction portion in the container, the flow-out portion arranged so that a supernatant of a solution in the container due to the introduction of the heavy solution is flowed out to the outside of the container, and a collector provided vertically below the discharge portion in the container, the collector collecting a component in the mixed sample, the component being lighter in specific gravity than the heavy solution from the supernatant flowed out from the flow-out portion.

公开(公告)号：US20170294297A1

公开(公告)日：2017-10-12

申请号：US15631286

申请日：2017-06-23

Applicant: SHIMADZU CORPORATION ,  KEIO UNIVERSITY

Inventor： Koretsugu OGATA ,  Kazuteru TAKAHASHI ,  Akiko KUBO ,  Makoto SUEMATSU ,  Takushi YAMAMOTO

IPC: H01J49/04

CPC classification number: H01J49/0418 ,  B05D1/60 ,  B05D3/107 ,  B05D7/5483 ,  H01J49/0004

Abstract: After a sample such as a biomedical tissue section is attached to an electrically-conductive slide glass (S1), the film layer of a matrix substance is appropriately formed by vapor deposition so as to cover the sample (S2). The crystal of the matrix substance in the film layer is very fine and uniform. Subsequently, the slide glass on which the matrix film layer is formed is placed in a vaporized solvent atmosphere, and the solvent infiltrates into the matrix film layer (S3). When the solvent sufficiently infiltrated is vaporized, a substance to be measured in the sample takes in the matrix and re-crystallized. Furthermore, the matrix film layer is formed again on the surface by the vapor deposition (S4). The added matrix film layer absorbs excessive energy of a laser beam during MALDI, which suppresses the denaturation of the substance to be measured and the like, so that high detection sensitivity can be achieved while high spatial resolution is maintained.

公开(公告)号：US20160247669A1

公开(公告)日：2016-08-25

申请号：US15049366

申请日：2016-02-22

Applicant: SHIMADZU CORPORATION

Inventor： Yusuke TATEISHI ,  Kazuteru TAKAHASHI ,  Hideki SATO

IPC: H01J49/14

CPC classification number: H01J49/147 ,  H01J27/024 ,  H01J27/205 ,  H01J49/067 ,  H01J49/145

Abstract: In an ion source 3 in which a repeller electrode 32 for forming a repelling electric field that repels ions toward an ion emission port 311 is provided inside of an ionization chamber 31, ion focusing electrodes 36 and 37 are respectively arranged between an electron introduction port 312 and a filament 34 and between an electron discharge port 313 and a counter filament 35. An electric field formed by applying a predetermined voltage to each of the ion focusing electrodes 36 and 37 intrudes into the ionization chamber 31 through the electron introduction port 312 and the electron discharge port 313, and becomes a focusing electric field that pushes the ions in an ion optical axis C direction. Ions at positions off a central part of the ionization chamber 31 receive the combined force of the force of the repelling electric field and the force of the focusing electric field, and move toward the ion emission port 311 while approaching the ion optical axis C. Accordingly, the amount of ions sent out from the ion emission port increases. Further, even if a charge-up phenomenon occurs, the ion trajectories less easily change, and the stability of the sensitivity can be enhanced.

Abstract translation: 在离子源3中，在电离室31的内部设置有用于形成排斥离子的排斥电极的排斥电极32，离子聚焦电极36,37分别配置在电子引入口312 灯丝34和电子排出口313和反向灯丝35之间。通过向每个离子聚焦电极36和37施加预定电压而形成的电场通过电子引入口312侵入电离室31和 电子放电端口313，并且成为在离子光轴C方向推动离子的聚焦电场。 在离子化室31的中心部分的位置处的离子接收排斥电场的力和聚焦电场的力的组合力，并且在接近离子光轴C的同时向离子发射端口311移动。因此 从离子发射口发出的离子量增加。 此外，即使发生充电现象，离子轨迹也不容易改变，并且可以提高灵敏度的稳定性。

公开(公告)号：US20160035553A1

公开(公告)日：2016-02-04

申请号：US14781950

申请日：2014-04-04

Applicant: SHIMADZU CORPORATION ,  KEIO UNIVERSITY

Inventor： Koretsugu OGATA ,  Kazuteru TAKAHASHI ,  Akiko KUBO ,  Makoto SUEMATSU ,  Takuji YAMAMOTO

IPC: H01J49/04 ,  B05D1/00 ,  B05D3/10 ,  B05D7/00

CPC classification number: H01J49/0418 ,  B05D1/60 ,  B05D3/107 ,  B05D7/5483 ,  H01J49/0004

Abstract: After a sample such as a biomedical tissue section is attached to an electrically-conductive slide glass (S1), the film layer of a matrix substance is appropriately formed by vapor deposition so as to cover the sample (S2). The crystal of the matrix substance in the film layer is very fine and uniform. Subsequently, the slide glass on which the matrix film layer is formed is placed in a vaporized solvent atmosphere, and the solvent infiltrates into the matrix film layer (S3). When the solvent sufficiently infiltrated is vaporized, a substance to be measured in the sample takes in the matrix and re-crystallized. Furthermore, the matrix film layer is formed again on the surface by the vapor deposition (S4). The added matrix film layer absorbs excessive energy of a laser beam during MALDI, which suppresses the denaturation of the substance to be measured and the like, so that high detection sensitivity can be achieved while high spatial resolution is maintained.

Abstract translation: 将诸如生物医学组织切片的样品附接到导电载玻片（S1）之后，通过气相沉积适当地形成基质物质的膜层以覆盖样品（S2）。 膜层中的基质物质的晶体非常细且均匀。 随后，将形成有基质膜层的载片玻璃置于汽化溶剂气氛中，溶剂浸入基质膜层（S3）。 当充分渗透的溶剂蒸发时，样品中待测量的物质进入基质并重结晶。 此外，通过气相沉积在表面上再次形成基质膜层（S4）。 添加的基质膜层在MALDI期间吸收激光束的过量能量，抑制待测物质的变性等，从而在保持高空间分辨率的同时可以实现高检测灵敏度。