公开(公告)号：US20240417621A1

公开(公告)日：2024-12-19

申请号：US18818982

申请日：2024-08-29

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Xin LI ,  Clive A. SMITH ,  Alexandra CLAY ,  Colin PILKINGTON

IPC: C09K23/18 ,  B01D11/04 ,  B01D17/04 ,  B01D17/12 ,  B01L3/00 ,  C08G65/00 ,  C09K23/00 ,  C09K23/16

Abstract: A surfactant of formula (I): A-(L1)a-(CH2)b-(L2)c-X  (I) wherein A is a perfluoropolyether; L1 is CONR′, wherein R′ is selected from H and C1-6 alkyl; a is 0 or 1; b is 0 or an integer between 1 and 10; L2 is a linking group; c is 0 or 1; and X is a charged group.

公开(公告)号：US20180133715A1

公开(公告)日：2018-05-17

申请号：US15578455

申请日：2016-06-03

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Frank F. CRAIG ,  Marian REHAK ,  David HOLMES ,  Clive A. SMITH ,  Xin LIU ,  Giuseppe BENAZZI ,  Xin LI ,  Vinayaka PAWATE

IPC: B01L3/00 ,  G01N35/08 ,  G01N15/14

CPC classification number: B01L3/502784 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0627 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/0644 ,  G01N15/1484 ,  G01N35/08 ,  G01N2015/1481 ,  G01N2015/149

Abstract: Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.

公开(公告)号：US20200316545A1

公开(公告)日：2020-10-08

申请号：US16768121

申请日：2018-11-29

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Xin LI ,  Clive A. SMITH ,  Alexandra CLAY ,  Colin PILKINGTON

IPC: B01F17/00 ,  C08G65/00 ,  B01D17/04 ,  B01D17/12 ,  B01D11/04

Abstract: A surfactant of formula (I): A-(L1)a-(C2)b-(L2)c-X   (I) whereinA is a perfluoropolyether;L1 is CONR', wherein R′ is selected from H and C1-6 alkyl;a is 0 or 1;b is 0 or an integer between 1 and 10;L2 is a linking group;c is 0 or 1; andX is a charged group.

公开(公告)号：US20160169788A1

公开(公告)日：2016-06-16

申请号：US14907936

申请日：2014-07-30

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Clive A. SMITH ,  Xin Li ,  Karing ENESA ,  Xin LIU ,  Frank F. CRAIG ,  Graeme WHYTE

IPC: G01N15/14

CPC classification number: G01N15/1484 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  G01N2015/1481 ,  G01N2015/149

Abstract: The present invention provides an emulsion for sorting droplets in a microfluidic device, the emulsion comprising: a discontinuous aqueous phase; and a continuous oil phase; wherein said aqueous phase comprises at least one analyte, said oil phase comprises a fluorous oil and a refractive index modifying compound comprising at least one aromatic ring, and the refractive index of said aqueous phase and the refractive index of said oil phase are substantially matched.

Abstract translation: 本发明提供了一种用于在微流体装置中分选液滴的乳液，所述乳液包括：不连续的水相; 和连续油相; 其中所述水相包含至少一种分析物，所述油相包括含氟油和包含至少一个芳环的折射率改性化合物，并且所述水相的折射率和所述油相的折射率基本匹配。

公开(公告)号：US20160252446A1

公开(公告)日：2016-09-01

申请号：US14907867

申请日：2014-07-30

Applicant: Sphere Fluidics Limited

Inventor： Clive A. SMITH ,  Xin LI ,  Karine Enesa ,  Xin LIU ,  Frank F. CRAIG ,  Graeme WHYTE

IPC: G01N15/14

CPC classification number: G01N15/1434 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  C12Q1/04 ,  C12Q1/06 ,  G01N15/1425 ,  G01N21/49 ,  G01N2015/0065 ,  G01N2015/149 ,  Y10T436/2575

Abstract: We describe a method of label-free cell and/or particle sorting in a microfluidic device, the method comprising; providing a stream of aqueous droplets in oil in a channel of said microfluidic device, wherein at least some of said droplets include cells and/or particles illuminating said stream from a first direction; detecting, for example, scattered light from cells and/or particles within said aqueous droplets in a second direction; determining, for example, a number of said cells and/or particles in each droplet from the scattered light; and sorting said aqueous droplets into one of a plurality of differentiated streams responsive to said determined number. The method further comprises modifying a refractive index of said oil to more closely match a refractive index of said aqueous droplets to reduce light scattered from boundaries of said droplets. Optionally a volume of the droplets is also controlled. In embodiments the oil is fluorous oil. We describe examples of preferred refractive index modifying compounds.

Abstract translation: 我们描述了在微流体装置中无标记细胞和/或颗粒分选的方法，该方法包括： 在所述微流体装置的通道中提供油中的水滴液滴，其中至少一些所述液滴包括从第一方向照射所述流的细胞和/或颗粒; 从第二方向检测来自所述水滴中的细胞和/或颗粒的散射光; 确定例如来自散射光的每个液滴中的所述细胞和/或颗粒的数量; 以及响应于所述确定的数量将所述水滴分选成多个分化流中的一个。 该方法还包括改变所述油的折射率以更紧密地匹配所述水滴的折射率，以减少从所述液滴的边界散射的光。 任选地，还控制一定体积的液滴。 在实施方案中，油是氟油。 我们描述优选折射率修饰化合物的实例。