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    PRODUCTION OF PROTEINS AND POLYPEPTIDES
    1.
    发明申请
    PRODUCTION OF PROTEINS AND POLYPEPTIDES 审中-公开

    公开(公告)号：US20170283474A1

    公开(公告)日：2017-10-05

    申请号：US15490328

    申请日：2017-04-18

    Applicant: SPIBER TECHNOLOGIES AB

    Inventor： Jan JOHANSSON ,  Anna RISING ,  My HEDHAMMAR ,  Kerstin NORDLING

    IPC: C07K14/435 ,  C12N15/62

    CPC classification number: C07K14/43518 ,  C12N15/62

    Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.

    SPIDER SILK PROTEINS AND METHODS FOR PRODUCING SPIDER SILK PROTEINS
    3.
    发明申请
    SPIDER SILK PROTEINS AND METHODS FOR PRODUCING SPIDER SILK PROTEINS 有权
    天丝蛋白和生产天丝蛋白的方法

    公开(公告)号：US20140079674A1

    公开(公告)日：2014-03-20

    申请号：US14086244

    申请日：2013-11-21

    Applicant: Spiber Technologies AB

    Inventor： Jan JOHANSSON ,  Goran HJALM ,  Margareta STARK ,  Anna RISING ,  Stefan GRIP ,  Wilhelm ENGSTROM ,  My HEDHAMMAR

    IPC: C07K14/435 ,  A61K47/42 ,  A61K35/12

    CPC classification number: C07K14/43518 ,  A61K35/12 ,  A61K47/42 ,  D01F4/00

    Abstract: The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.

    Abstract translation: 本发明提供了一种分离的主要的安瓿蛋白质宿主蛋白，其由150至420个氨基酸残基组成，并由式REP-CT定义。 REP是具有80至300个氨基酸残基的重复的N末端衍生的蛋白质片段。 CT是具有70至120个氨基酸残基的C末端衍生的蛋白质片段。 本发明进一步提供了由第一蛋白片段（其是主要的安非他索特灵素蛋白质）和包含融合配偶体和切割剂识别位点的第二蛋白质片段组成的分离的融合蛋白质。 第一个蛋白质片段通过所述切割剂识别位点连接到融合配偶体。 本发明还提供了一种生产主要的安非他命复合蛋白及其聚合物的方法。

    ENGINEERED SPIDER SILK PROTEINS AND USES THEREOF
    4.
    发明申请
    ENGINEERED SPIDER SILK PROTEINS AND USES THEREOF 有权

    公开(公告)号：US20230070786A1

    公开(公告)日：2023-03-09

    申请号：US17895320

    申请日：2022-08-25

    Applicant: SPIBER TECHNOLOGIES AB

    Inventor： Anna RISING ,  Jan JOHANSSON ,  Marlene ANDERSSON

    IPC: C07K14/435 ,  C12N5/00

    Abstract: A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D.

    SPIDER SILK FUSION PROTEIN STRUCTURES FOR BINDING TO AN ORGANIC TARGET
    5.
    发明申请
    SPIDER SILK FUSION PROTEIN STRUCTURES FOR BINDING TO AN ORGANIC TARGET 审中-公开
    用于绑定到有机目标的SPIDER SILK融合蛋白质结构

    公开(公告)号：US20150291674A1

    公开(公告)日：2015-10-15

    申请号：US14753101

    申请日：2015-06-29

    Applicant: SPIBER TECHNOLOGIES AB

    Inventor： My HEDHAMMAR ,  Jan JOHANSSON ,  Anna RISING ,  Per Åke NYGREN

    IPC: C07K14/435 ,  C07K14/76 ,  B01D15/20 ,  C07K14/31 ,  C12N5/00 ,  C07K16/06 ,  C07D495/04

    CPC classification number: C07K14/43518 ,  B01D15/20 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/281 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C07K16/065 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/70 ,  C07K2319/705 ,  C12N5/0068 ,  C12N2533/50 ,  Y10S210/905

    Abstract: A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

    Abstract translation: 提供能够与有机靶相互选择性的蛋白质结构。 蛋白质结构是包含能够与有机靶选择性相互作用的重组融合蛋白作为重复结构单元的聚合物。 融合蛋白包含部分B，REP和CT，以及任选的NT。 B是超过30个氨基酸残基的非宿主部分，其提供与有机靶的选择性相互作用的能力。 REP是70至300个氨基酸残基的部分，并衍生自蜘蛛丝蛋白的重复片段。 CT是70至120个氨基酸残基的部分，衍生自蜘蛛丝蛋白的C末端片段。 NT是100至160个氨基酸残基的任选部分，并衍生自蜘蛛丝蛋白的N-末端片段。 其融合蛋白质和蛋白质结构可用作亲和介质和细胞支架材料。

    CHARGE-REVERSED N-TERMINAL SPIDER SILK PROTEIN DOMAIN AND USES THEREOF
    6.
    发明申请
    CHARGE-REVERSED N-TERMINAL SPIDER SILK PROTEIN DOMAIN AND USES THEREOF 审中-公开

    公开(公告)号：US20200207819A1

    公开(公告)日：2020-07-02

    申请号：US16816486

    申请日：2020-03-12

    Applicant: SPIBER TECHNOLOGIES AB

    Inventor： Jan JOHANSSON ,  Anna RISING ,  Nina KRONQVIST ,  Kerstin NORDLING

    IPC: C07K14/435 ,  C07K1/36

    Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.

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