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    MUTANT MICROORGANISMS HAVING A HIGH ABILITY TO PRODUCE PUTRESCINE AND METHOD FOR PRODUCING PUTRESCINE USING THE SAME
    3.
    发明申请
    MUTANT MICROORGANISMS HAVING A HIGH ABILITY TO PRODUCE PUTRESCINE AND METHOD FOR PRODUCING PUTRESCINE USING THE SAME 有权
    具有生产聚氨酯的高生产能力的突变微生物及使用其生产聚氨酯的方法

    公开(公告)号：US20100203599A1

    公开(公告)日：2010-08-12

    申请号：US12579052

    申请日：2009-10-14

    申请人： Sang Yup Lee ,  Zhi Gang Qian ,  Xiaoxia Xia ,  Yong Jae Jeon

    发明人： Sang Yup Lee ,  Zhi Gang Qian ,  Xiaoxia Xia ,  Yong Jae Jeon

    IPC分类号： C12P13/00 ,  C12N15/74 ,  C12N1/00 ,  C12N1/21

    CPC分类号： C12P13/001

    摘要： Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications.

    摘要翻译： 提供具有产生高浓度腐胺的能力的突变微生物，其中涉及腐胺降解或利用途径的基因被灭活或缺失，其制备方法。 还提供了通过培养突变微生物以高产率产生腐胺的方法。 突变体微生物可用于生产高浓度腐胺，可广泛用于各种工业应用。

    METHOD OF PREPARING PROTEIN HAVING HIGH CONTENT OF SPECIFIC AMINO ACID BY CO-EXPRESSION WITH TRNA OF SPECIFIC AMINO ACID
    4.
    发明申请
    METHOD OF PREPARING PROTEIN HAVING HIGH CONTENT OF SPECIFIC AMINO ACID BY CO-EXPRESSION WITH TRNA OF SPECIFIC AMINO ACID 审中-公开
    具有特异性氨基酸的高含量的蛋白质的制备方法与特异性氨基酸的TRNA通过共表达

    公开(公告)号：US20110244515A1

    公开(公告)日：2011-10-06

    申请号：US13120404

    申请日：2009-09-22

    申请人： Sang Yup Lee ,  Xiaoxia Xia ,  Zhi Gang Qian ,  Jong Hwan Baek

    发明人： Sang Yup Lee ,  Xiaoxia Xia ,  Zhi Gang Qian ,  Jong Hwan Baek

    IPC分类号： C12P21/00 ,  C12N1/21 ,  C12N15/63

    CPC分类号： C12N15/67 ,  C07K14/43518 ,  C07K14/43586

    摘要： The present invention relates to a method of increasing the expression of a target protein by co-expression of a gene encoding a target protein having a high content of a specific amino acid with a nucleotide sequence encoding the tRNA of the specific amino acid. According to the present invention, the expression of a protein having a high content of a specific amino acid can be remarkably increased by co-expression with the tRNA of the specific amino acid. Thus, the present invention is useful for increasing the productivity of a protein having a high content of a specific amino acid, such as a repetitive protein.

    摘要翻译： 本发明涉及通过将编码具有高特异性氨基酸含量的靶蛋白的基因与编码特定氨基酸的tRNA的核苷酸序列共表达来增加靶蛋白的表达的方法。 根据本发明，通过与特定氨基酸的tRNA共表达，可以显着提高具有高特异性氨基酸含量的蛋白质的表达。 因此，本发明对于提高具有高含量的特定氨基酸的蛋白质如重复蛋白质的生产率是有用的。

    HIGH-MOLECULAR-WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN AND MICRO- OR NANO-SIZED SPIDER SILK OR SILK-LIKE FIBER PRODUCED THEREFROM
    6.
    发明申请
    HIGH-MOLECULAR-WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN AND MICRO- OR NANO-SIZED SPIDER SILK OR SILK-LIKE FIBER PRODUCED THEREFROM 审中-公开
    高分子量重量丝素或丝状蛋白质和微米或纳米尺寸的天花丝绸或丝状纤维生产

    公开(公告)号：US20120231499A1

    公开(公告)日：2012-09-13

    申请号：US13124818

    申请日：2011-03-11

    申请人： Sang Yup Lee ,  Xiaoxia Xia ,  Zhi Gang Qian ,  Jeong Wook Lee ,  Young Hwan Park

    发明人： Sang Yup Lee ,  Xiaoxia Xia ,  Zhi Gang Qian ,  Jeong Wook Lee ,  Young Hwan Park

    IPC分类号： C12P21/02 ,  D01D5/06 ,  C07K14/00

    CPC分类号： C07K14/43518 ,  D01D5/0007 ,  D01D5/06 ,  D01F4/02

    摘要： A high-molecular-weight recombinant silk or silk-like protein having a molecular weight which is substantially similar to that of native silk protein, and a micro- or nano-sized spider silk or silk-like fiber having improved physical properties, produced therefrom. The recombinant silk or silk-like protein according to the invention has high molecular weight, like dragline silk proteins from spiders, while a fiber produced therefrom has excellent physical properties compared to a fiber produced from native silk protein. Thus, the recombinant silk or silk-like protein and the spider silk or silk-like fiber produced therefrom will be highly useful in various industrial applications, including bioengineering applications and medical applications.

    摘要翻译： 具有与天然丝蛋白基本相似的分子量的高分子量重组丝或丝状蛋白质，以及由其制备的具有改善的物理性质的微米或纳米尺寸的蜘蛛丝或丝状纤维 。 根据本发明的重组丝或丝状蛋白具有高分子量，如来自蜘蛛的拉丝丝蛋白，而由其制备的纤维与由天然丝蛋白产生的纤维相比具有优异的物理性能。 因此，重组丝或丝状蛋白质以及由其生产的蜘蛛丝或丝状纤维在各种工业应用中非常有用，包括生物工程应用和医疗应用。