公开(公告)号：US06875591B1

公开(公告)日：2005-04-05

申请号：US09631709

申请日：2000-08-03

申请人： Satoshi Koizumi ,  Hiroshi Nagano ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Hiroshi Nagano ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

IPC分类号： C12P19/32 ,  C12P19/30 ,  C12P19/02

CPC分类号： C12P19/32

摘要： Processes for producing GDP-fucose, comprising allowing GKDM and a culture broth of a microorganism capable of converting GKDM into GDP-fucose to be present in an aqueous medium, forming and accumulating GDP-fucose therein, and recovering the GDP-fucose therefrom; or comprising allowing a GTP precursor, a saccharide, a culture broth of a microorganism capable of forming GTP from a GTP precursor, and a culture broth of a microorganism capable of forming GKDM from a saccharide and GTP to be present in an aqueous medium, forming and accumulating GKDM therein, converting the accumulated GKDM into GDP-fucose using as a culture broth of a microorganism capable of converting GKDM into GDP-fucose to form and accumulate GDP-fucose therein, and recovering the GDP-fucose therefrom; and a process for producing GKDM, comprising allowing a GTP precursor, a saccharide, a culture broth of a microorganism capable of forming GTP from a GTP precursor, and a culture broth of a microorganism capable forming GKDM from a saccharide and GTP to be present in an aqueous medium, forming and accumulating GKDM therein, and recovering the GKDM therefrom.

摘要翻译： 生产GDP-岩藻糖的方法，包括使GKDM和能够将GKDM转化为GDP-岩藻糖的微生物的培养液存在于水性介质中，形成并积累其中的GDP-岩藻糖，并从其中回收GDP-岩藻糖; 或者包括使GTP前体，糖类，能够从GTP前体形成GTP的微生物的培养液和能够从糖和GTP形成GKDM的微生物的培养液存在于水性介质中，形成 并在其中积累GKDM，使用能够将GKDM转化为GDP-岩藻糖的微生物的培养液将积累的GKDM转化为GDP-岩藻糖，以形成和积累GDP-岩藻糖，并从其中回收GDP-岩藻糖; 以及生产GKDM的方法，包括使来自GTP前体的能够形成GTP的微生物的GTP前体，糖类，培养液以及能够从糖和GTP形成GKDM的微生物的培养液存在于 水介质，在其中形成和积聚GKDM，并从中回收GKDM。

公开(公告)号：US06946281B2

公开(公告)日：2005-09-20

申请号：US10378745

申请日：2003-03-04

申请人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

IPC分类号： C07K14/195 ,  C12N1/21 ,  C12N9/90 ,  C12N15/61 ,  C12P19/26 ,  C12N19/24

CPC分类号： C12P19/26 ,  C07K14/195 ,  C12N9/90

摘要： The present invention provides a novel protein having N-acetylglucosamine 2-epimerase activity; a DNA encoding the protein; a recombinant vector containing the DNA; a transformant obtainable by introducing the recombinant vector into a host cell; and a process for producing the protein or N-acetylmannosamine using the transformant.

摘要翻译： 本发明提供具有N-乙酰葡糖胺2-差向异构酶活性的新型蛋白质; 编码蛋白质的DNA; 含有DNA的重组载体; 通过将重组载体导入宿主细胞获得的转化体; 以及使用该转化体生产蛋白质或N-乙酰甘露糖胺的方法。

公开(公告)号：US20050019874A1

公开(公告)日：2005-01-27

申请号：US10940026

申请日：2004-09-14

申请人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

IPC分类号： C12P19/30 ,  C12N9/10

CPC分类号： C12P19/30

摘要： This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme source; and a process for producing N-acetylglucosamine-1-phosphate, in which a culture broth of a microorganism having strong galactokinase activity, or a treated product of the culture broth, is used as the enzyme source.

公开(公告)号：US06830908B2

公开(公告)日：2004-12-14

申请号：US10218381

申请日：2002-08-15

申请人： Satoshi Koizumi ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

IPC分类号： C12N910

CPC分类号： C12N9/1051 ,  C12N9/1048 ,  C12P19/18 ,  Y10S435/822

摘要： The present invention provides a protein having &bgr;1,4-galactosyltransferase activity, DNA encoding the protein, a recombinant DNA comprising the DNA, a transformant carrying the recombinant DNA, a process for producing &bgr;1,4-galactosyltransferase by using the transformant, and a process for producing a galactose-containing carbohydrate by using the transformant.

摘要翻译： 本发明提供了具有β1,4-半乳糖基转移酶活性的蛋白质，编码该蛋白质的DNA，包含DNA的重组DNA，携带重组DNA的转化体，使用该转化体产生β1,4-半乳糖基转移酶的方法， 用于通过使用转化体产生含半乳糖的碳水化合物。

公开(公告)号：US06673581B1

公开(公告)日：2004-01-06

申请号：US09958548

申请日：2001-10-11

申请人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

IPC分类号： C12P1924

CPC分类号： C12P19/24 ,  C12N9/90 ,  C12Y503/01007

摘要： The present invention provides a novel protein having mannose isomerase activity, DNA encoding the protein, a recombinant vector comprising the DNA, a transformant obtained by introducing the recombinant vector into a host cell, and processes for producing the above protein and mannose, fructose, xylulose and lyxose by using the transformant.

摘要翻译： 本发明提供了具有甘露糖异构酶活性的新型蛋白质，编码该蛋白质的DNA，包含DNA的重组载体，将重组载体导入宿主细胞获得的转化体，以及产生上述蛋白质和甘露糖，果糖，木酮糖的方法 并通过使用转化体来研究。

公开(公告)号：US07579175B2

公开(公告)日：2009-08-25

申请号：US10338872

申请日：2003-01-09

申请人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

IPC分类号： C12P7/58 ,  C12P1/00 ,  C12Q1/00 ,  C12N9/24 ,  C12N1/20 ,  C02F3/34 ,  C07K14/00 ,  C07H21/04

CPC分类号： C12P19/26

摘要： The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.

摘要翻译： 本发明提供了经济地生产N-乙酰神经氨酸的方法，而不使用昂贵的材料如丙酮酸和磷酸烯醇丙酮酸。 该方法包括：（i）具有N-乙酰神经氨酸醛缩酶活性或N-乙酰神经氨酸合成酶活性的微生物的培养物或培养物的处理物质，（ii）能够产生丙酮酸的微生物培养物或 培养物的处理物质，或能够产生磷酸烯醇丙酮酸或培养物处理物质的微生物的培养物，（iii）N-乙酰甘露糖胺，和（iv）形成丙酮酸所必需的能量源或 磷酸烯醇式丙酮酸存在于水性介质中以在水性介质中形成并积聚N-乙酰神经氨酸; 并从水性介质中回收N-乙酰神经氨酸。

公开(公告)号：US06964858B2

公开(公告)日：2005-11-15

申请号：US09907574

申请日：2001-07-19

申请人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

IPC分类号： C12P19/30 ,  C12P1/00 ,  C07H21/04 ,  C12N9/00

CPC分类号： C12P19/30

摘要： This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme source; and a process for producing N-acetylglucosamine-1-phosphate, in which a culture broth of a microorganism having strong galactokinase activity, or a treated product of the culture broth, is used as the enzyme source.

摘要翻译： 本发明涉及一种制备糖核苷酸的方法，其中a）从核苷酸前体产生NTP的微生物的培养液或培养液的处理产物，和b）能够培养的微生物的培养液 从糖和NTP生产糖核苷酸或培养液的处理产物用作酶源; 复合碳水化合物的制造方法，其中上述a）和b）和c）能够从糖核苷酸和复合碳水化合物制备复合碳水化合物的微生物，动物细胞或昆虫细胞的培养液 前体或培养液的处理产物用作酶源; 复合碳水化合物的制造方法，其中可以从糖核苷酸和复合碳水化合物前体生产复合碳水化合物的微生物，动物细胞或昆虫细胞的培养液或培养液的处理产物是 作为酶源; 使用其中具有强烈的半乳糖激酶活性的微生物的培养液或培养液的处理产物的N-乙酰葡糖胺-1-磷酸的生产方法作为酶源。

公开(公告)号：US06846656B1

公开(公告)日：2005-01-25

申请号：US09645321

申请日：2000-08-25

申请人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N1/21 ,  C12N9/00 ,  C12N9/88 ,  C12P19/26 ,  C12R1/15 ,  C12R1/19 ,  C12R1/85 ,  C12P7/58 ,  C07H21/04 ,  C12N1/20 ,  C12P21/06

CPC分类号： C12P19/26

摘要： The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.

摘要翻译： 本发明提供了经济地生产N-乙酰神经氨酸的方法，而不使用昂贵的材料如丙酮酸和磷酸烯醇丙酮酸。 该方法包括：（i）具有N-乙酰神经氨酸醛缩酶活性或N-乙酰神经氨酸合成酶活性的微生物的培养物或培养物的处理物质，（ii）能够产生丙酮酸的微生物培养物或 培养物的处理物质，或能够产生磷酸烯醇丙酮酸或培养物处理物质的微生物的培养物，（iii）N-乙酰甘露糖胺，和（iv）形成丙酮酸所必需的能量源或 磷酸烯醇式丙酮酸存在于水性介质中以在水性介质中形成并积聚N-乙酰神经氨酸; 并从水性介质中回收N-乙酰神经氨酸。

公开(公告)号：US06821756B2

公开(公告)日：2004-11-23

申请号：US09068528

申请日：1998-05-13

申请人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

IPC分类号： C12P1930

CPC分类号： C12P19/30

摘要： This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme source; and a process for producing N-acetylglucosamine-1-phosphate, in which a culture broth of a microorganism having strong galactokinase activity, or a treated product of the culture broth, is used as the enzyme source.

摘要翻译： 本发明涉及一种制备糖核苷酸的方法，其中a）从核苷酸前体产生NTP的微生物的培养液或培养液的处理产物，和b）能够培养的微生物的培养液 从糖和NTP生产糖核苷酸或培养液的处理产物用作酶源; 复合碳水化合物的制造方法，其中上述a）和b）和c）能够从糖核苷酸和复合碳水化合物生产复合碳水化合物的微生物，动物细胞或昆虫细胞的培养液 前体或培养液的处理产物用作酶源; 复合碳水化合物的制造方法，其中可以从糖核苷酸和复合碳水化合物前体生产复合碳水化合物的微生物，动物细胞或昆虫细胞的培养液或培养液的处理产物是 作为酶源; 使用其中具有强烈的半乳糖激酶活性的微生物的培养液或培养液的处理产物的N-乙酰葡糖胺-1-磷酸的生产方法作为酶源。

公开(公告)号：US08435763B2

公开(公告)日：2013-05-07

申请号：US10940026

申请日：2004-09-14

申请人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

发明人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

IPC分类号： C12P19/30 ,  C12P19/00 ,  C12P1/00 ,  C12P39/00 ,  C12N1/00 ,  C12N1/20

CPC分类号： C12P19/30

摘要： This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme source; and a process for producing N-acetylglucosamine-1-phosphate, in which a culture broth of a microorganism having strong galactokinase activity, or a treated product of the culture broth, is used as the enzyme source.