公开(公告)号：US5262309A

公开(公告)日：1993-11-16

申请号：US668517

申请日：1991-03-22

申请人： Satoshi Nakamura ,  Masami Fukuoka ,  Tsukio Masegi ,  Kazuo Kitai ,  Arata Kato ,  Yataro Ichikawa

发明人： Satoshi Nakamura ,  Masami Fukuoka ,  Tsukio Masegi ,  Kazuo Kitai ,  Arata Kato ,  Yataro Ichikawa

IPC分类号： A61K38/00 ,  C07K14/525 ,  C12N1/21 ,  C12P21/06 ,  C07K13/00 ,  C12N15/00

CPC分类号： C07K14/525 ,  A61K38/00

摘要： This invention relates to a novel physiologically active polypeptide containing modified amino acids on both terminals at the amino-terminus and the carboxy-terminus in the amino acid sequence of the human tumor neclosis factor (human TNF), a recombinant plasmid containing a DNA region encoding the polypeptide, a recombinant microorganism cell transformed with the recombinant plasmid, a method of producing the polypeptide by cultivating the microorganism cell, a pharmaceutical composition containing the polypeptide and a method of purifying and recovering the polypeptide. The polypeptide in this invention has excellent antitumor activity compared to the human TNF and can be utilized as an active ingredient of a pharmaceutical composition having antitumor activity.

摘要翻译： PCT No.PCT / JP89 / 00966 Sec。 371日期1991年3月22日 102（e）1991年3月22日PCT申请1989年9月22日PCT公开号 出版物WO90 / 03395 日本1994年4月5日。本发明涉及在人肿瘤坏死因子（人TNF）的氨基酸序列的氨基末端和羧基末端两端含有修饰氨基酸的新的生理活性多肽， 含有编码该多肽的DNA区域的重组质粒，用重组质粒转化的重组微生物细胞，通过培养微生物细胞产生该多肽的方法，含该多肽的药物组合物以及纯化和回收该多肽的方法。 本发明的多肽与人TNF相比具有优异的抗肿瘤活性，可用作具有抗肿瘤活性的药物组合物的活性成分。

公开(公告)号：US5028420A

公开(公告)日：1991-07-02

申请号：US235576

申请日：1988-07-26

申请人： Tsukio Masegi ,  Satoshi Nakamura ,  Kazuo Kitai ,  Masami Fukuoka ,  Kenji Yone ,  Arata Kato ,  Jun Suzuki ,  Noriyuki Tsunekawa ,  Yataro Ichikawa

发明人： Tsukio Masegi ,  Satoshi Nakamura ,  Kazuo Kitai ,  Masami Fukuoka ,  Kenji Yone ,  Arata Kato ,  Jun Suzuki ,  Noriyuki Tsunekawa ,  Yataro Ichikawa

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P35/00 ,  C07K1/22 ,  C07K14/00 ,  C07K14/52 ,  C07K14/525 ,  C12N1/20 ,  C12N15/28 ,  C12P21/02 ,  C12R1/19

CPC分类号： C07K14/525 ,  A61K38/00

摘要： A novel physiologically active polypeptide having antitumor activity obtained by improving human tumor necrosis factor (TNF). The amino acid sequence of the polypeptide essentially corresponds to that of human tumor necrosis factor, except that ten amino acids Nos. 1 to 10 are deleted and (Met).sub.n -Arg-Lys-Arg are added to the amino (NH.sub.2)-terminus, where n is 0 or 1. Other amino acid modifications can also be made to the polypeptide while retaining high antitumor activity. The novel polypeptide has less side effects that the natural-type TNF, particularly with respect to cytotoxicity. A a DNA fragment encoding the polypeptide, a recombinant plasmid containing the DNA fragment, a recombinant microorganism cell transformed with the plasmid, a method of producing a novel physiologically active polypeptide using the microorganism cell, and a pharmaceutical composition comprising the polypeptide as an active ingredient are also provided.

公开(公告)号：US5075236A

公开(公告)日：1991-12-24

申请号：US186078

申请日：1988-04-25

申请人： Kenji Yone ,  Jun Suzuki ,  Noriyuki Tsunekawa ,  Arata Kato ,  Satoshi Nakamura ,  Tsukio Masegi ,  Kazuo Kitai ,  Yataro Ichikawa

发明人： Kenji Yone ,  Jun Suzuki ,  Noriyuki Tsunekawa ,  Arata Kato ,  Satoshi Nakamura ,  Tsukio Masegi ,  Kazuo Kitai ,  Yataro Ichikawa

IPC分类号： G01N33/53 ,  C07K14/005 ,  C07K14/195 ,  C07K16/24 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577

CPC分类号： C07K16/241 ,  C07K2317/34 ,  Y10S436/811 ,  Y10S436/815

摘要： A method of confirming the diagnosis of Kawasaki disease in a patient which comprises assaying the patient's body fluid for the presence of elevated levels of a substance specifically bound by an anti-tumor necrosis factor monoclonal antibody.