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    METHOD OF RECOVERING LIPASE ACTIVITY
    2.
    发明申请
    METHOD OF RECOVERING LIPASE ACTIVITY 有权
    回收脂肪酶活性的方法

    公开(公告)号：US20090075349A1

    公开(公告)日：2009-03-19

    申请号：US12266617

    申请日：2008-11-07

    申请人： Satoshi NEGISHI ,  Junko Suzuki ,  Yoshie Yamauchi

    发明人： Satoshi NEGISHI ,  Junko Suzuki ,  Yoshie Yamauchi

    IPC分类号： C12P7/64 ,  C12N9/98 ,  C12N11/14

    CPC分类号： C12N9/20 ,  C11C3/02 ,  C11C3/10 ,  C12N11/14 ,  C12P7/62 ,  C12P7/6454

    摘要： The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived from Thermomyces sp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived from Thermomyces sp. and immobilized on a carrier which is crushed into the average particle size of 1 μm or larger and smaller than 300 μm in an esterification or transesterification reaction; and washing said lipase or lipase powder composition with triacylglycerol. According to this method, the decreased lipase activity can be effectively recovered.

    摘要翻译： 本发明公开了一种回收脂肪酶活性的方法，该方法包括以下步骤：使用源自Thermomyces sp。 并固定在载体上，或包含助滤剂和源自Thermomyces sp。的脂肪酶的脂肪酶粉末组合物。 并固化在酯化或酯交换反应中被粉碎成平均粒径为1μm以上且小于300μm的载体; 并用三酰基甘油洗涤所述脂肪酶或脂肪酶粉末组合物。 根据该方法，可以有效地回收脂肪酶活性降低。

    Method for producing lipase powder compositions
    3.
    发明授权
    Method for producing lipase powder compositions 有权
    生产脂肪酶粉末组合物的方法

    公开(公告)号：US08911980B2

    公开(公告)日：2014-12-16

    申请号：US13260142

    申请日：2010-03-26

    申请人： Junko Suzuki ,  Yoshie Yamauchi ,  Yuko Toyama ,  Satoshi Negishi

    发明人： Junko Suzuki ,  Yoshie Yamauchi ,  Yuko Toyama ,  Satoshi Negishi

    IPC分类号： C12N11/12 ,  C12N9/98 ,  C12N11/02 ,  C12N11/10 ,  C12N11/14 ,  C12N9/20

    CPC分类号： C12N9/98 ,  C12N9/20 ,  C12N11/02 ,  C12N11/10 ,  C12N11/12 ,  C12N11/14 ,  C12Y301/01003

    摘要： The present invention provides a method for producing a ground product of a lipase powder composition comprising an immobilized lipase in which a lipase derived from Thermomyces sp. is immobilized to a silica carrier and a hydrophilic powder other than a silica carrier, the method comprising the steps of wetting the hydrophilic powder with oil; separating the hydrophilic powder wetted with oil from the oil to obtain an oil-containing hydrophilic powder; mixing the oil-containing hydrophilic powder and the immobilized lipase at a ratio of 0.45:1 to 0.85:1 based on mass of the hydrophilic powder and the immobilized lipase before oil wetting; and grinding the obtained mixture, the steps being conducted in the above order.

    摘要翻译： 本发明提供一种生产包含固定化脂肪酶的脂肪酶粉末组合物的研磨产物的方法，其中来自Thermomyces sp。 固定在二氧化硅载体和除二氧化硅载体以外的亲水性粉末，该方法包括用油润湿亲水性粉末的步骤; 分离从油中润湿的亲水性粉末，得到含油亲水性粉末; 以油润湿前的亲水性粉末和固定化脂肪酶的质量，以0.45:1〜0.85:1的比例混合含油亲水性粉末和固定化脂肪酶; 并研磨所得混合物，所述步骤按照上述顺序进行。

    METHOD FOR PRODUCING LIPASE POWDER COMPOSITIONS
    4.
    发明申请
    METHOD FOR PRODUCING LIPASE POWDER COMPOSITIONS 有权
    生产脂肪酶粉末组合物的方法

    公开(公告)号：US20120021483A1

    公开(公告)日：2012-01-26

    申请号：US13260142

    申请日：2010-03-26

    申请人： Junko Suzuki ,  Yoshie Yamauchi ,  Yuko Toyama ,  Satoshi Negishi

    发明人： Junko Suzuki ,  Yoshie Yamauchi ,  Yuko Toyama ,  Satoshi Negishi

    IPC分类号： C12N11/14

    CPC分类号： C12N9/98 ,  C12N9/20 ,  C12N11/02 ,  C12N11/10 ,  C12N11/12 ,  C12N11/14 ,  C12Y301/01003

    摘要： The present invention provides a method for producing a ground product of a lipase powder composition comprising an immobilized lipase in which a lipase derived from Thermomyces sp. is immobilized to a silica carrier and a hydrophilic powder other than a silica carrier, the method comprising the steps of wetting the hydrophilic powder with oil; separating the hydrophilic powder wetted with oil from the oil to obtain an oil-containing hydrophilic powder; mixing the oil-containing hydrophilic powder and the immobilized lipase at a ratio of 0.45:1 to 0.85:1 based on mass of the hydrophilic powder and the immobilized lipase before oil wetting; and grinding the obtained mixture, the steps being conducted in the above order.

    摘要翻译： 本发明提供一种生产包含固定化脂肪酶的脂肪酶粉末组合物的研磨产物的方法，其中来自Thermomyces sp。 固定在二氧化硅载体和除二氧化硅载体以外的亲水性粉末，该方法包括用油润湿亲水性粉末的步骤; 分离从油中润湿的亲水性粉末，得到含油亲水性粉末; 以油润湿前的亲水性粉末和固定化脂肪酶的质量，以0.45:1〜0.85:1的比例混合含油亲水性粉末和固定化脂肪酶; 并研磨所得混合物，所述步骤按照上述顺序进行。

    Method of recovering lipase activity
    5.
    发明授权
    Method of recovering lipase activity 有权
    回收脂肪酶活性的方法

    公开(公告)号：US07923224B2

    公开(公告)日：2011-04-12

    申请号：US12266617

    申请日：2008-11-07

    申请人： Satoshi Negishi ,  Junko Suzuki ,  Yoshie Yamauchi

    发明人： Satoshi Negishi ,  Junko Suzuki ,  Yoshie Yamauchi

    IPC分类号： C12P7/64 ,  C12N9/98 ,  C12N11/14

    CPC分类号： C12N9/20 ,  C11C3/02 ,  C11C3/10 ,  C12N11/14 ,  C12P7/62 ,  C12P7/6454

    摘要： The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived from Thermomyces sp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived from Thermomyces sp. and immobilized on a carrier which is crushed into the average particle size of 1 μm or larger and smaller than 300 μm in an esterification or transesterification reaction; and washing said lipase or lipase powder composition with triacylglycerol. According to this method, the decreased lipase activity can be effectively recovered.

    摘要翻译： 本发明公开了一种回收脂肪酶活性的方法，该方法包括以下步骤：使用源自Thermomyces sp。 并固定在载体上，或包含助滤剂和源自Thermomyces sp。的脂肪酶的脂肪酶粉末组合物。 并固化在酯化或酯交换反应中被粉碎成1μm以上且小于300μm的平均粒径的载体上; 并用三酰基甘油洗涤所述脂肪酶或脂肪酶粉末组合物。 根据该方法，可以有效地回收脂肪酶活性降低。

    Optical resolution for producing optically active alcohol
    9.
    发明授权
    Optical resolution for producing optically active alcohol 失效
    用于制造光学活性醇的光学分辨率

    公开(公告)号：US5696299A

    公开(公告)日：1997-12-09

    申请号：US529059

    申请日：1995-09-15

    申请人： Satoshi Negishi ,  Seiichi Shirasawa ,  Junko Suzuki ,  Yukie Masuda

    发明人： Satoshi Negishi ,  Seiichi Shirasawa ,  Junko Suzuki ,  Yukie Masuda

    IPC分类号： C12P7/62 ,  C12P41/00 ,  C07C33/18 ,  C07C33/46

    CPC分类号： C12P7/62 ,  C12P41/004

    摘要： A process for producing an optically active alcohol comprising carrying out interesterification between a racemic alcohol and an ester selected from the group consisting of (a) a diester between a lower monohydric alcohol and a saturated dicarboxylic acid having 14 or more carbon atoms, (b) a triglyceride of a saturated fatty acid having 16 or more carbon atoms, and (c) a monoester between a lower monohydric alcohol and a saturated fatty acid having 18 or more carbon atoms in the presence of lipase, preferably heat-resistant lipase, and in the presence or absence of a solvent, preferably in the absence of a solvent, under a substantially water-free condition, separating an optically active alcohol rich in either one of R- and S-forms from the reaction mixture, and adding an optically inactive non-racemic alcohol to the residue of the previous step to carry out interesterification under the same conditions as in the previous reaction to separate the other enantiomer. According to the present invention a racemic alcohol can easily be resolved into each enantiomer with high purity in good yield.

    摘要翻译： 一种光学活性醇的制造方法，其包括在外消旋醇与选自（a）低级一元醇和具有14个或更多个碳原子的饱和二羧酸之间的二酯的酯中进行酯交换，（b） 具有16个或更多个碳原子的饱和脂肪酸的甘油三酯，和（c）在脂肪酶，优选耐热脂肪酶存在下，具有18个或更多个碳原子的低级一元醇和饱和脂肪酸之间的单酯，以及 在基本无水条件下，优选在不存在溶剂的情况下存在或不存在溶剂，从反应混合物中分离富含R-和S-形式的任何一种的光学活性醇，并加入光学惰性 将非外消旋醇与前述步骤的残余物进行酯交换，在与之前反应相同的条件下进行酯交换以分离另一种对映异构体。 根据本发明，外消旋醇可以容易地以良好的产率分离成高纯度的每种对映异构体。

    Transesterification method using lipase powder with a particle diameter of 20-50 microns
    10.
    发明授权
    Transesterification method using lipase powder with a particle diameter of 20-50 microns 失效
    使用粒径为20-50微米的脂肪酶粉末的酯交换方法

    公开(公告)号：US5480787A

    公开(公告)日：1996-01-02

    申请号：US296921

    申请日：1994-08-31

    申请人： Satoshi Negishi ,  Seiichi Shirasawa ,  Junko Suzuki ,  Tateo Murui

    发明人： Satoshi Negishi ,  Seiichi Shirasawa ,  Junko Suzuki ,  Tateo Murui

    IPC分类号： C12N9/20 ,  C12N11/14 ,  C12P7/62 ,  C12P7/64 ,  C12R1/05

    CPC分类号： C12P7/6454 ,  C12N11/14

    摘要： The transesterification method is conducted with a powdery lipase wherein the powdery lipase is dispersed in ester-containing starting materials in the presence or absence of an inert organic solvent, and the diameter of at least 90% of the dispersed lipase particles is kept in the range of 1 to 100 .mu.m in the course of the trans-esterification reaction. A reactivity higher than that realized by the conventional transesterification method with a powdery lipase is obtained and the lipase can be easily recovered and reused, without causing a loss of the lipase activity, lowering of the conversion due to the residence of the substrate on the immobilizing carrier and side reactions caused by water introduced into the reaction system by the immobilizing carrier.

    摘要翻译： 酯交换方法用粉状脂肪酶进行，其中在存在或不存在惰性有机溶剂的情况下将粉状脂肪酶分散在含酯原料中，并且至少90％的分散的脂肪酶颗粒的直径保持在 在反式酯化反应过程中为1〜100微米。 获得比通过常规酯交换法用粉末脂肪酶实现的更高的反应性，并且可以容易地回收和再利用脂肪酶，而不会引起脂肪酶活性的丧失，由于底物停留在固定化上的转化率降低 由固定载体引入反应体系的水引起的载体和副反应。