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    METHOD OF RECOVERING LIPASE ACTIVITY
    3.
    发明申请
    METHOD OF RECOVERING LIPASE ACTIVITY 有权
    回收脂肪酶活性的方法

    公开(公告)号：US20090075349A1

    公开(公告)日：2009-03-19

    申请号：US12266617

    申请日：2008-11-07

    申请人： Satoshi NEGISHI ,  Junko Suzuki ,  Yoshie Yamauchi

    发明人： Satoshi NEGISHI ,  Junko Suzuki ,  Yoshie Yamauchi

    IPC分类号： C12P7/64 ,  C12N9/98 ,  C12N11/14

    CPC分类号： C12N9/20 ,  C11C3/02 ,  C11C3/10 ,  C12N11/14 ,  C12P7/62 ,  C12P7/6454

    摘要： The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived from Thermomyces sp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived from Thermomyces sp. and immobilized on a carrier which is crushed into the average particle size of 1 μm or larger and smaller than 300 μm in an esterification or transesterification reaction; and washing said lipase or lipase powder composition with triacylglycerol. According to this method, the decreased lipase activity can be effectively recovered.

    摘要翻译： 本发明公开了一种回收脂肪酶活性的方法，该方法包括以下步骤：使用源自Thermomyces sp。 并固定在载体上，或包含助滤剂和源自Thermomyces sp。的脂肪酶的脂肪酶粉末组合物。 并固化在酯化或酯交换反应中被粉碎成平均粒径为1μm以上且小于300μm的载体; 并用三酰基甘油洗涤所述脂肪酶或脂肪酶粉末组合物。 根据该方法，可以有效地回收脂肪酶活性降低。

    Method for producing lipase powder compositions
    4.
    发明授权
    Method for producing lipase powder compositions 有权
    生产脂肪酶粉末组合物的方法

    公开(公告)号：US08911980B2

    公开(公告)日：2014-12-16

    申请号：US13260142

    申请日：2010-03-26

    申请人： Junko Suzuki ,  Yoshie Yamauchi ,  Yuko Toyama ,  Satoshi Negishi

    发明人： Junko Suzuki ,  Yoshie Yamauchi ,  Yuko Toyama ,  Satoshi Negishi

    IPC分类号： C12N11/12 ,  C12N9/98 ,  C12N11/02 ,  C12N11/10 ,  C12N11/14 ,  C12N9/20

    CPC分类号： C12N9/98 ,  C12N9/20 ,  C12N11/02 ,  C12N11/10 ,  C12N11/12 ,  C12N11/14 ,  C12Y301/01003

    摘要： The present invention provides a method for producing a ground product of a lipase powder composition comprising an immobilized lipase in which a lipase derived from Thermomyces sp. is immobilized to a silica carrier and a hydrophilic powder other than a silica carrier, the method comprising the steps of wetting the hydrophilic powder with oil; separating the hydrophilic powder wetted with oil from the oil to obtain an oil-containing hydrophilic powder; mixing the oil-containing hydrophilic powder and the immobilized lipase at a ratio of 0.45:1 to 0.85:1 based on mass of the hydrophilic powder and the immobilized lipase before oil wetting; and grinding the obtained mixture, the steps being conducted in the above order.

    摘要翻译： 本发明提供一种生产包含固定化脂肪酶的脂肪酶粉末组合物的研磨产物的方法，其中来自Thermomyces sp。 固定在二氧化硅载体和除二氧化硅载体以外的亲水性粉末，该方法包括用油润湿亲水性粉末的步骤; 分离从油中润湿的亲水性粉末，得到含油亲水性粉末; 以油润湿前的亲水性粉末和固定化脂肪酶的质量，以0.45:1〜0.85:1的比例混合含油亲水性粉末和固定化脂肪酶; 并研磨所得混合物，所述步骤按照上述顺序进行。

    METHOD FOR PRODUCING LIPASE POWDER COMPOSITIONS
    5.
    发明申请
    METHOD FOR PRODUCING LIPASE POWDER COMPOSITIONS 有权
    生产脂肪酶粉末组合物的方法

    公开(公告)号：US20120021483A1

    公开(公告)日：2012-01-26

    申请号：US13260142

    申请日：2010-03-26

    申请人： Junko Suzuki ,  Yoshie Yamauchi ,  Yuko Toyama ,  Satoshi Negishi

    发明人： Junko Suzuki ,  Yoshie Yamauchi ,  Yuko Toyama ,  Satoshi Negishi

    IPC分类号： C12N11/14

    CPC分类号： C12N9/98 ,  C12N9/20 ,  C12N11/02 ,  C12N11/10 ,  C12N11/12 ,  C12N11/14 ,  C12Y301/01003

    摘要： The present invention provides a method for producing a ground product of a lipase powder composition comprising an immobilized lipase in which a lipase derived from Thermomyces sp. is immobilized to a silica carrier and a hydrophilic powder other than a silica carrier, the method comprising the steps of wetting the hydrophilic powder with oil; separating the hydrophilic powder wetted with oil from the oil to obtain an oil-containing hydrophilic powder; mixing the oil-containing hydrophilic powder and the immobilized lipase at a ratio of 0.45:1 to 0.85:1 based on mass of the hydrophilic powder and the immobilized lipase before oil wetting; and grinding the obtained mixture, the steps being conducted in the above order.

    摘要翻译： 本发明提供一种生产包含固定化脂肪酶的脂肪酶粉末组合物的研磨产物的方法，其中来自Thermomyces sp。 固定在二氧化硅载体和除二氧化硅载体以外的亲水性粉末，该方法包括用油润湿亲水性粉末的步骤; 分离从油中润湿的亲水性粉末，得到含油亲水性粉末; 以油润湿前的亲水性粉末和固定化脂肪酶的质量，以0.45:1〜0.85:1的比例混合含油亲水性粉末和固定化脂肪酶; 并研磨所得混合物，所述步骤按照上述顺序进行。

    Method of recovering lipase activity
    6.
    发明授权
    Method of recovering lipase activity 有权
    回收脂肪酶活性的方法

    公开(公告)号：US07923224B2

    公开(公告)日：2011-04-12

    申请号：US12266617

    申请日：2008-11-07

    申请人： Satoshi Negishi ,  Junko Suzuki ,  Yoshie Yamauchi

    发明人： Satoshi Negishi ,  Junko Suzuki ,  Yoshie Yamauchi

    IPC分类号： C12P7/64 ,  C12N9/98 ,  C12N11/14

    CPC分类号： C12N9/20 ,  C11C3/02 ,  C11C3/10 ,  C12N11/14 ,  C12P7/62 ,  C12P7/6454

    摘要： The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived from Thermomyces sp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived from Thermomyces sp. and immobilized on a carrier which is crushed into the average particle size of 1 μm or larger and smaller than 300 μm in an esterification or transesterification reaction; and washing said lipase or lipase powder composition with triacylglycerol. According to this method, the decreased lipase activity can be effectively recovered.

    摘要翻译： 本发明公开了一种回收脂肪酶活性的方法，该方法包括以下步骤：使用源自Thermomyces sp。 并固定在载体上，或包含助滤剂和源自Thermomyces sp。的脂肪酶的脂肪酶粉末组合物。 并固化在酯化或酯交换反应中被粉碎成1μm以上且小于300μm的平均粒径的载体上; 并用三酰基甘油洗涤所述脂肪酶或脂肪酶粉末组合物。 根据该方法，可以有效地回收脂肪酶活性降低。

    Conjugated fatty acid containing monoglycerides and process for producing them
    9.
    发明授权
    Conjugated fatty acid containing monoglycerides and process for producing them 失效
    含有单酸甘油酯的共轭脂肪酸及其制备方法

    公开(公告)号：US07220873B2

    公开(公告)日：2007-05-22

    申请号：US10210207

    申请日：2002-08-02

    申请人： Yoshie Yamauchi ,  Takaya Yamamoto ,  Kentaro Tsutsumi ,  Yuji Shimada ,  Yomi Watanabe ,  Akio Sugihara ,  Yoshio Tominaga

    发明人： Yoshie Yamauchi ,  Takaya Yamamoto ,  Kentaro Tsutsumi ,  Yuji Shimada ,  Yomi Watanabe ,  Akio Sugihara ,  Yoshio Tominaga

    IPC分类号： C11C3/00

    CPC分类号： C11C3/025 ,  A23D9/013 ,  C11C1/10 ,  C11C3/02 ,  C12P7/6472

    摘要： This invention provides monoglycerides containing a conjugated fatty acid, and a process for producing the above described conjugated fatty acid containing monoglycerides in which all kinds of lipases can be used as catalysts.This invention relate to monoglycerides containing a conjugated fatty acid (preferably in an amount of 50% or more of fatty acids).This invention also relates to a process for producing the conjugated fatty acid containing monoglycerides, in which the conjugated fatty acid containing a free fatty acid and glycerol are subjected to the reaction of esterification, or of esterification and glycerolysis in the presence of lipase as a catalyst.

    摘要翻译： 本发明提供含有共轭脂肪酸的单酸甘油酯，以及含有单酸甘油酯的上述共轭脂肪酸的制造方法，其中可以使用各种脂肪酶作为催化剂。 本发明涉及含有共轭脂肪酸的单酸甘油酯（优选为脂肪酸的50％以上的量）。 本发明还涉及含有单酸甘油酯的共轭脂肪酸的制造方法，其中含有游离脂肪酸和甘油的共轭脂肪酸在脂肪酶作为催化剂存在下进行酯化反应或酯化和甘油分解 。