公开(公告)号：US09249436B2

公开(公告)日：2016-02-02

申请号：US13365253

申请日：2012-02-02

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

IPC分类号： C12P7/64 ,  C12R1/89 ,  C12N1/12 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N15/52

CPC分类号： C12P7/6463 ,  C07C69/52 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C10L2290/30 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  Y02E50/13

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US20120277452A1

公开(公告)日：2012-11-01

申请号：US13547457

申请日：2012-07-12

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

IPC分类号： C12P7/64 ,  C12N1/13 ,  C07C69/732

CPC分类号： C12P7/6463 ,  C07C69/52 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C10L2290/30 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  Y02E50/13

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US20120203018A1

公开(公告)日：2012-08-09

申请号：US13365253

申请日：2012-02-02

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

IPC分类号： C12P7/64 ,  C07C57/02 ,  C12N1/13

CPC分类号： C12P7/6463 ,  C07C69/52 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C10L2290/30 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  Y02E50/13

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US08852885B2

公开(公告)日：2014-10-07

申请号：US13547457

申请日：2012-07-12

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

IPC分类号： C12N1/13 ,  C12P33/06 ,  C12P7/64 ,  C12N15/113 ,  C12N9/02 ,  C12N15/52 ,  C12N9/16 ,  C12N9/10

CPC分类号： C12P7/6463 ,  C07C69/52 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C10L2290/30 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  Y02E50/13

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US20130034887A1

公开(公告)日：2013-02-07

申请号：US13650024

申请日：2012-10-11

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

IPC分类号： C12P7/64

CPC分类号： C12P7/6463 ,  C07C69/52 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C10L2290/30 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  Y02E50/13

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US09279136B2

公开(公告)日：2016-03-08

申请号：US13630757

申请日：2012-09-28

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

IPC分类号： C12N9/16 ,  C12N1/13 ,  C12N15/67 ,  C12N15/79 ,  C12N15/82 ,  C12P7/64 ,  A23D7/00 ,  A23D9/00 ,  A23K1/16 ,  C11B1/00 ,  C11C3/00

CPC分类号： C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

摘要： Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产食品组合物，油，燃料，油脂化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造食品组合物，以及运输燃料如可再生柴油，生物柴油和可再生喷气燃料， 以及诸如功能流体，表面活性剂，肥皂和润滑剂的油脂化学品。

公开(公告)号：US09109239B2

公开(公告)日：2015-08-18

申请号：US13650018

申请日：2012-10-11

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

IPC分类号： A23L2/00 ,  A23D7/00 ,  A23D9/00 ,  A23C9/12 ,  C11C1/00 ,  C07C51/00 ,  C11C3/00 ,  A01N55/06 ,  A61K31/305 ,  A01N37/00 ,  C12P7/64 ,  A23K1/16 ,  C11B1/00

CPC分类号： C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

摘要： Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产食品组合物，油，燃料，油脂化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造食品组合物，以及输送燃料如可再生柴油，生物柴油和可再生喷气燃料， 以及诸如功能流体，表面活性剂，肥皂和润滑剂的油脂化学品。

公开(公告)号：US08765424B2

公开(公告)日：2014-07-01

申请号：US13543666

申请日：2012-07-06

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

IPC分类号： C12P7/64 ,  C12P1/00 ,  C07C53/00 ,  C10L1/18

CPC分类号： C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造运输燃料如可再生柴油，生物柴油和可再生喷气燃料，以及油脂化学品 例如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US20130102039A1

公开(公告)日：2013-04-25

申请号：US13630757

申请日：2012-09-28

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

IPC分类号： C12P7/64

CPC分类号： C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

摘要： Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产食品组合物，油，燃料，油脂化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造食品组合物，以及运输燃料如可再生柴油，生物柴油和可再生喷气燃料， 以及诸如功能流体，表面活性剂，肥皂和润滑剂的油脂化学品。

公开(公告)号：US20110294174A1

公开(公告)日：2011-12-01

申请号：US13118365

申请日：2011-05-27

申请人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

IPC分类号： C12P7/64 ,  C09K3/00 ,  C12N1/13 ,  C12N1/21 ,  C12N1/19 ,  C10L1/232 ,  C12P1/00

CPC分类号： C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造运输燃料如可再生柴油，生物柴油和可再生喷气燃料，以及油脂化学品 例如功能流体，表面活性剂，肥皂和润滑剂。