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1.发明授权Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids 有权突变型HPGG基序和HDASH基序delta-5去饱和酶及其在制备多不饱和脂肪酸中的应用公开(公告)号：US08658413B2
公开(公告)日：2014-02-25
申请号：US13218673
申请日：2011-08-26
申请人： Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12N9/001 , C12P7/6472 , C12Y103/01035
摘要： Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b5-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”], are also disclosed.
摘要翻译： 具有将dihomo-γ-亚麻酸转化的能力的变体delta-5去饱和酶[DGLA; 20：3ω-6]花生四烯酸[ARA; 20：4ω-6]和/或二十碳四烯酸[ETA; 20：4ω-3]至二十碳五烯酸[EPA; 20：5ω-3]，并且在细胞色素b5样结构域的HPGG（SEQ ID NO：7）基序和HDASH（SEQ ID NO：8）基序内的至少一个突变中具有至少一个突变。 还公开了分离的核酸片段和包含编码Δ-5去饱和酶的片段的重组构建物，以及制备长链多不饱和脂肪酸[“PUFA”]的方法。
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2.发明申请公开(公告)号：US20120052537A1
公开(公告)日：2012-03-01
申请号：US13218708
申请日：2011-08-26
申请人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12N9/1029 , C12N9/0071 , C12N15/815 , C12P7/6472
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [“EPA”], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.
摘要翻译： 本文公开了含油酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其能够产生包含大于25重量％的二十碳五烯酸[“EPA”]的微生物油，其为以干细胞重量的重量百分比测量的ω-3多不饱和脂肪酸 。
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3.发明申请HIGH EICOSAPENTAENOIC ACID OILS FROM IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA 有权来自改良的YARROWIA LIPOLYTICA优化菌株的高选择性酸性油公开(公告)号：US20100317735A1
公开(公告)日：2010-12-16
申请号：US12814880
申请日：2010-06-14
IPC分类号： A61K31/202 , A61P9/12 , A61P3/10 , A61P3/06 , A61P29/00 , A23L1/30 , A23K1/00 , A23D9/02 , C12N1/19
CPC分类号： C12N9/1029 , A21D2/165 , A23K20/158 , C12N9/00 , C12N9/13 , C12N9/93 , C12P7/6427 , C12P7/6472 , C12P7/6481
摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。 除了其他异源＆Dgr。9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱饱和酶，Dgr。12去饱和酶，C16外，这些菌株过度表达至少一种Dgr 9延伸酶/ / 18延伸酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 该菌株具有至少一种过氧化物酶体生物发生因子蛋白质敲除。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。
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4.发明授权Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production 有权优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产公开(公告)号：US08637298B2
公开(公告)日：2014-01-28
申请号：US12814815
申请日：2010-06-14
IPC分类号： C12N1/00
CPC分类号： C12N9/1029 , A21D2/165 , A23K20/158 , C12N9/00 , C12N9/13 , C12N9/93 , C12P7/6427 , C12P7/6472 , C12P7/6481
摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其是以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。 除了其他异源Delta9延伸酶，Delta8去饱和酶，Δ5去饱和酶，Δ17去饱和酶，Δ12去饱和酶，C16 / 18延长酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A，这些菌株过表达至少一种Δ9延伸酶/Δδ去饱和酶多酶 合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 至少一种过氧化物酶体生物因子蛋白的表达下调。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。
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5.发明授权公开(公告)号：US08703473B2
公开(公告)日：2014-04-22
申请号：US13218708
申请日：2011-08-26
申请人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12N9/1029 , C12N9/0071 , C12N15/815 , C12P7/6472
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [“EPA”], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.
摘要翻译： 本文公开了含油酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其能够产生包含大于25重量％的二十碳五烯酸[“EPA”]的微生物油，其为以干细胞重量的重量百分比测量的ω-3多不饱和脂肪酸 。
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6.发明授权High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica 有权来自改良的解脂耶氏酵母菌株的高二十碳五烯酸油公开(公告)号：US08399226B2
公开(公告)日：2013-03-19
申请号：US12814880
申请日：2010-06-14
CPC分类号： C12N9/1029 , A21D2/165 , A23K20/158 , C12N9/00 , C12N9/13 , C12N9/93 , C12P7/6427 , C12P7/6472 , C12P7/6481
摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[EPA]，ω-3多不饱和脂肪酸的脂肪酵母解脂耶氏酵母的工程菌株，以总脂肪酸的重量％[％TFA]和 具有至少3.1％的EPA％TFA与亚油酸的比例，以％TFA测定。 除了其他异源＆Dgr .9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱饱和酶，Dgr。12去饱和酶，C16外，这些菌株过度表达至少一种Dgr 9延伸酶/ / 18延伸酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 该菌株具有至少一种过氧化物酶体生物发生因子蛋白质敲除。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。
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7.发明申请MUTANT HPGG MOTIF AND HDASH MOTIF DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS 有权MUTANT HPGG MOTIF和HDASH MOTIF DELTA-5 DESATURASES及其在制备聚氨酯脂肪酸中的应用公开(公告)号：US20120052549A1
公开(公告)日：2012-03-01
申请号：US13218673
申请日：2011-08-26
申请人： Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12N9/001 , C12P7/6472 , C12Y103/01035
摘要： Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b5-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”], are also disclosed.
摘要翻译： 具有将dihomo-γ-亚麻酸转化的能力的变体delta-5去饱和酶[DGLA; 20：3ω-6]花生四烯酸[ARA; 20：4ω-6]和/或二十碳四烯酸[ETA; 20：4ω-3]至二十碳五烯酸[EPA; 20：5ω-3]，并且在细胞色素b5样结构域的HPGG（SEQ ID NO：7）基序和HDASH（SEQ ID NO：8）基序内的至少一个突变中具有至少一个突变。 还公开了分离的核酸片段和包含编码Δ-5去饱和酶的片段的重组构建物，以及制备长链多不饱和脂肪酸[“PUFA”]的方法。
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8.发明申请公开(公告)号：US20100317072A1
公开(公告)日：2010-12-16
申请号：US12814815
申请日：2010-06-14
CPC分类号： C12N9/1029 , A21D2/165 , A23K20/158 , C12N9/00 , C12N9/13 , C12N9/93 , C12P7/6427 , C12P7/6472 , C12P7/6481
摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。 除了其他异源＆Dgr .9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱饱和酶，Dgr。12去饱和酶，C16外，这些菌株过度表达至少一种Dgr 9延伸酶/ / 18延伸酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 至少一种过氧化物酶体生物因子蛋白的表达下调。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。
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公开(公告)号：US08518674B2
公开(公告)日：2013-08-27
申请号：US12893469
申请日：2010-09-29
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于25％二十碳五烯酸（EPA，ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包括表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使EPA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内生产EPA的方法。
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10.发明授权Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production 有权优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产公开(公告)号：US08323935B2
公开(公告)日：2012-12-04
申请号：US12244822
申请日：2008-10-03
申请人： Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
发明人： Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
IPC分类号： C12P7/64 , C07D293/10
CPC分类号： C12P7/6427 , C12N9/0083 , C12N9/1029 , C12N15/815 , C12P7/6472
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, in the total oil fraction are described. These strains over-express heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases and C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Preferred gene knockouts are described, as are methods for producing EPA within the host cells and products comprising EPA from the optimized Yarrowia lipolytica strains.
摘要翻译： 描述了能够在总油馏分中产生大于50重量％的二十碳五烯酸[EPA]（ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株过表达异源＆Dgr。9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱氢酶，Dgr。12去饱和酶和C16 / 18延伸酶，以及任选的过表达二酰基甘油胆碱磷酸转移酶。 描述优选的基因敲除，以及在宿主细胞内生产EPA的方法以及来自优化的解脂耶氏酵母菌株的包含EPA的产物的方法。
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