公开(公告)号：US20210198325A1

公开(公告)日：2021-07-01

申请号：US17169504

申请日：2021-02-07

Applicant: ShanghaiTech University

Inventor： Chao Zhong ,  Jiaofang Huang ,  Suying Liu ,  Chen Zhang

IPC: C07K14/32 ,  C12N11/10

Abstract: Three-dimensional, living, self-regenerative structures of predetermined geometry comprising solidified print material comprising a biofilm of Bacillus subtilis comprise a TasA-R protein, wherein R is a recombinant, heterologous functional group, wherein the TasA-R provides a preferably tunable physiochemical property like viscosity, reactivity, affinity as a function of the R group.

公开(公告)号：US20230295023A1

公开(公告)日：2023-09-21

申请号：US18067732

申请日：2022-12-19

Applicant: ShanghaiTech University

Inventor： Chao Zhong ,  Jiahua Pu ,  Yi Liu

IPC: C02F1/68 ,  C02F1/50

CPC classification number: C02F1/683 ,  C02F1/50 ,  C02F2101/10

Abstract: A living engineered biofilm material comprises microbial cells embedded in a protective extracellular matrix comprising a fusion protein of an amyloid domain and a contaminant binding domain operative to bind a contaminant of a water source, and thereby facilitate decontamination of the water source.

公开(公告)号：US20210071016A1

公开(公告)日：2021-03-11

申请号：US17092261

申请日：2020-11-07

Applicant: ShanghaiTech University

Inventor： Chao Zhong ,  Yingfeng Li ,  Ke Li

IPC: C09D11/04 ,  C07K14/245 ,  C07K14/00 ,  B29C39/00 ,  B29C39/22

Abstract: A material fabrication method comprises (a) fabricating a structure from a programmable amyloid material (PAM) ink comprising an amyloid monomer stabilized in a liquid solvent; and (b) contacting the structure with an agent which triggers polymerization of the amyloid monomer and stabilization of the structure.

公开(公告)号：US12012519B2

公开(公告)日：2024-06-18

申请号：US17169581

申请日：2021-02-08

Applicant: ShanghaiTech University

Inventor： Chao Zhong ,  Mengkui Cui

IPC: C12N15/62 ,  B82Y30/00 ,  C07K14/435 ,  C09D189/00

CPC classification number: C09D189/00 ,  C07K14/435 ,  C07K2319/20

Abstract: Liquid-liquid phase separation (LLPS) driven protein-based underwater adhesive coatings are made from a dimeric protein comprising a marine adhesive protein (MAP) domain and a liquid-liquid phase separation-mediating low complexity (LC) domain.

公开(公告)号：US20210079049A1

公开(公告)日：2021-03-18

申请号：US17106105

申请日：2020-11-28

Applicant: ShanghaiTech University

Inventor： Chao Zhong ,  Chen Zhang ,  Jiaofang Huang ,  Jicong Zhang

IPC: C07K14/32 ,  C07K14/435 ,  C07K14/245 ,  C07K14/21 ,  C07K14/195 ,  C09J7/10 ,  C09J11/08 ,  C09J189/00 ,  C09J105/00

Abstract: Engineered living glues made by bacteria biofilms integrate natural marine adhesive proteins and electrostatic interactions into bacterial biofilm components such as amyloid protein TasA, surface layer protein BslA, and exopolysaccharides.

公开(公告)号：US11970621B2

公开(公告)日：2024-04-30

申请号：US17092261

申请日：2020-11-07

Applicant: ShanghaiTech University

Inventor： Chao Zhong ,  Yingfeng Li ,  Ke Li

IPC: C09D11/04 ,  B29C39/00 ,  B29C39/22 ,  C07K14/00 ,  C07K14/245

CPC classification number: C09D11/04 ,  B29C39/006 ,  B29C39/22 ,  C07K14/001 ,  C07K14/245 ,  B29K2089/00

Abstract: A material fabrication method comprises (a) fabricating a structure from a programmable amyloid material (PAM) ink comprising an amyloid monomer stabilized in a liquid solvent; and (b) contacting the structure with an agent which triggers polymerization of the amyloid monomer and stabilization of the structure.

公开(公告)号：US20230220370A1

公开(公告)日：2023-07-13

申请号：US18178554

申请日：2023-03-06

Applicant: ShanghaiTech University

Inventor： Chao Zhong ,  Bolin An ,  Yanyi Wang ,  Xiaoyu Jiang

IPC: C12N11/04 ,  C12N15/70 ,  C12N15/63 ,  C12N9/02 ,  C07K14/435 ,  C09J189/00 ,  C07K14/245

CPC classification number: C12N11/04 ,  C12N15/70 ,  C12N15/635 ,  C12Y114/18001 ,  C12N9/0071 ,  C07K14/43504 ,  C09J189/00 ,  C07K14/245 ,  C12N2800/101

Abstract: A living engineered glue system for performing autonomous mechanical repairs comprises a biofilm of microbial cells embedded in an extracellular matrix and operably linked in an environmentally-inducible, cell-cell communication genetic circuit to control gene expression.

公开(公告)号：US11365224B2

公开(公告)日：2022-06-21

申请号：US17169504

申请日：2021-02-07

Applicant: ShanghaiTech University

Inventor： Chao Zhong ,  Jiaofang Huang ,  Suying Liu ,  Chen Zhang

IPC: C12N1/00 ,  C07K14/00 ,  C07K14/32 ,  C12N11/10

Abstract: Three-dimensional, living, self-regenerative structures of predetermined geometry comprising solidified print material comprising a biofilm of Bacillus subtilis comprise a TasA-R protein, wherein R is a recombinant, heterologous functional group, wherein the TasA-R provides a preferably tunable physiochemical property like viscosity, reactivity, affinity as a function of the R group.

公开(公告)号：US20210155826A1

公开(公告)日：2021-05-27

申请号：US17169581

申请日：2021-02-08

Applicant: ShanghaiTech University

Inventor： Chao Zhong ,  Mengkui Cui

IPC: C09D189/00 ,  C07K14/435

Abstract: Liquid-liquid phase separation (LLPS) driven protein-based underwater adhesive coatings are made from a dimeric protein comprising a marine adhesive protein (MAP) domain and a liquid-liquid phase separation-mediating low complexity (LC) domain.