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12 条记录 (耗时 0.025 秒)

    Process for production of human growth hormone using Bacillus Brevis
    1.
    发明授权
    Process for production of human growth hormone using Bacillus Brevis 失效
    使用芽孢杆菌生产人生长激素的方法

    公开(公告)号：US5714346A

    公开(公告)日：1998-02-03

    申请号：US507313

    申请日：1995-08-22

    申请人： Shigezo Udaka ,  Tsutomu Kajino ,  Yoko Saito ,  Masana Hirai ,  Yukio Yamada ,  Fumihiko Hoshino

    发明人： Shigezo Udaka ,  Tsutomu Kajino ,  Yoko Saito ,  Masana Hirai ,  Yukio Yamada ,  Fumihiko Hoshino

    IPC分类号： C07K14/61 ,  C12N1/21 ,  C12N15/75 ,  C12P21/06 ,  C07H21/04 ,  C12N1/20

    CPC分类号： C07K14/61 ,  C12N15/75 ,  C07K2319/02

    摘要： A host-vector system suitable for production of human growth hormone (hGH) and a process for production of hGH using the same are provided. As an hGH expression plasmid, a recombinant DNA wherein a DNA coding for hGH is linked to the 3'-terminal of a DNA containing a promoter region derived from Bacillus brevis is provided, and as a host, especially a mutant Bacillus brevis substantially not exhibiting protease activity to hGH is provided. A microorganism obtained by transforming said host with said hGH expression plasmid efficiently produces hGH when it is cultured.

    摘要翻译： PCT No.PCT / JP94 / 00269 Sec。 371日期：1995年8月22日 102（e）日期1995年8月22日PCT 1994年2月22日PCT PCT。 WO94 / 19474 PCT公开号 日期1995年9月1日提供适用于生产人生长激素（hGH）的宿主载体系统和使用其的生产hGH的方法。 作为hGH表达质粒，提供了重组DNA，其中编码hGH的DNA与含有源自短芽孢杆菌的启动子区的DNA的3'-末端连接，作为宿主，特别是基本上不显示的突变体短芽孢杆菌 提供了对hGH的蛋白酶活性。 通过用所述hGH表达质粒转化所述宿主获得的微生物在培养时有效地产生hGH。

    Polypeptide possessing protein disulfide isomerase activity gene encoding the same and process for producing the same
    2.
    发明授权
    Polypeptide possessing protein disulfide isomerase activity gene encoding the same and process for producing the same 失效
    具有编码相同蛋白质二硫键异构酶活性基因的多肽及其制备方法

    公开(公告)号：US5700659A

    公开(公告)日：1997-12-23

    申请号：US464365

    申请日：1995-06-05

    申请人： Yukio Yamada ,  Osamu Asami ,  Hidehiko Sugiyama ,  Chie Idekoba ,  Fumihiko Hoshino ,  Masana Hirai ,  Tsutomu Kajino ,  Takao Imaeda ,  Kiyoko Sarai

    发明人： Yukio Yamada ,  Osamu Asami ,  Hidehiko Sugiyama ,  Chie Idekoba ,  Fumihiko Hoshino ,  Masana Hirai ,  Tsutomu Kajino ,  Takao Imaeda ,  Kiyoko Sarai

    IPC分类号： C12N9/90 ,  C12N15/52 ,  C12N15/31

    CPC分类号： C12N9/90

    摘要： A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polyeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polylpeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.

    摘要翻译： 提供了具有蛋白质二硫键异构酶（PDI）活性的高度热稳定的多肽，编码多肽的基因和产生多肽的方法。 具有PDI活性的多肽的特征在于：A）具有催化蛋白质中二硫键交换的能力，B）主要以核糖核酸酶A为底物，C）具有20〜70℃的合适的活性温度，D）为 在pH值为6〜9的条件下稳定，E）分子量约为60,000〜62,000。 由于与常规PDI相比，具有较高的热稳定性并且在更宽的二硫苏糖醇浓度范围内表现出稳定的活性，因此可以提供可有利地用于某些蛋白质的重折叠反应的新型酶活性蛋白质。 此外，还提供了使用Humicola insolens或用含有上述基因的表达载体转化的转化体能够有效地产生具有PDI活性的多肽的方法。

    Polypeptide from Humicola insolens possessing protein disulfide isomerase activity gene encoding the same
    3.
    发明授权
    Polypeptide from Humicola insolens possessing protein disulfide isomerase activity gene encoding the same 失效
    来自Humicola insolens的多肽具有编码相同的蛋白质二硫键异构酶活性基因

    公开(公告)号：US5496719A

    公开(公告)日：1996-03-05

    申请号：US68395

    申请日：1993-05-27

    申请人： Yukio Yamada ,  Osamu Asami ,  Hidehiko Sugiyama ,  Chie Idekoba ,  Fumihiko Hoshino ,  Masana Hirai ,  Tsutomu Kajino ,  Takao Imaeda ,  Kiyoko Sarai

    发明人： Yukio Yamada ,  Osamu Asami ,  Hidehiko Sugiyama ,  Chie Idekoba ,  Fumihiko Hoshino ,  Masana Hirai ,  Tsutomu Kajino ,  Takao Imaeda ,  Kiyoko Sarai

    IPC分类号： C12N9/90 ,  A61K38/00 ,  C07K1/00 ,  C12N9/00

    CPC分类号： C12N9/90

    摘要： A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polypeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polypeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.

    摘要翻译： 提供了具有蛋白质二硫键异构酶（PDI）活性的高度热稳定的多肽，编码多肽的基因和产生多肽的方法。 具有PDI活性的多肽的特征在于：A）具有催化蛋白质中二硫键交换的能力，B）主要以核糖核酸酶A为底物，C）具有20〜70℃的合适的活性温度，D）为 在pH值为6〜9的条件下稳定，E）分子量约为60,000〜62,000。 由于与常规PDI相比，具有较高的热稳定性并且在更宽的二硫苏糖醇浓度范围内表现出稳定的活性，因此可以提供可有利地用于某些蛋白质的重折叠反应的新型酶活性蛋白质。 此外，还提供了能够使用Humicola insolens或使用含有上述基因的表达载体转化的转化体有效地产生具有PDI活性的多肽的方法。

    Valve structure of hydraulic shock absorber for vehicle
    7.
    发明授权
    Valve structure of hydraulic shock absorber for vehicle 失效
    汽车液压减震器阀门结构

    公开(公告)号：US06966411B2

    公开(公告)日：2005-11-22

    申请号：US10722233

    申请日：2003-11-25

    申请人： Tsutomu Kajino

    发明人： Tsutomu Kajino

    IPC分类号： B60G13/08 ,  F16F9/348

    CPC分类号： F16F9/3484

    摘要： In a valve structure of a hydraulic shock absorber for a vehicle structured by laminating a first leaf valve, a small-diameter leaf valve and a plurality of second leaf valves, an inner leaf valve is provided between a plurality of second leaf valves. An annular outer leaf valve having a larger thickness than that of the inner leaf valve is provided in an outer peripheral side of the inner leaf valve.

    摘要翻译： 在通过层压第一叶片阀，小直径叶片阀和多个第二叶片阀的用于车辆的液压减震器的阀结构中，在多个第二叶片阀之间设置有内叶片阀。 在内片阀的外周侧设置有比内片阀的厚度大的环状外侧叶片阀。