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    Polypeptide possessing protein disulfide isomerase activity gene encoding the same and process for producing the same
    1.
    发明授权
    Polypeptide possessing protein disulfide isomerase activity gene encoding the same and process for producing the same 失效
    具有编码相同蛋白质二硫键异构酶活性基因的多肽及其制备方法

    公开(公告)号：US5700659A

    公开(公告)日：1997-12-23

    申请号：US464365

    申请日：1995-06-05

    申请人： Yukio Yamada ,  Osamu Asami ,  Hidehiko Sugiyama ,  Chie Idekoba ,  Fumihiko Hoshino ,  Masana Hirai ,  Tsutomu Kajino ,  Takao Imaeda ,  Kiyoko Sarai

    发明人： Yukio Yamada ,  Osamu Asami ,  Hidehiko Sugiyama ,  Chie Idekoba ,  Fumihiko Hoshino ,  Masana Hirai ,  Tsutomu Kajino ,  Takao Imaeda ,  Kiyoko Sarai

    IPC分类号： C12N9/90 ,  C12N15/52 ,  C12N15/31

    CPC分类号： C12N9/90

    摘要： A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polyeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polylpeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.

    摘要翻译： 提供了具有蛋白质二硫键异构酶（PDI）活性的高度热稳定的多肽，编码多肽的基因和产生多肽的方法。 具有PDI活性的多肽的特征在于：A）具有催化蛋白质中二硫键交换的能力，B）主要以核糖核酸酶A为底物，C）具有20〜70℃的合适的活性温度，D）为 在pH值为6〜9的条件下稳定，E）分子量约为60,000〜62,000。 由于与常规PDI相比，具有较高的热稳定性并且在更宽的二硫苏糖醇浓度范围内表现出稳定的活性，因此可以提供可有利地用于某些蛋白质的重折叠反应的新型酶活性蛋白质。 此外，还提供了使用Humicola insolens或用含有上述基因的表达载体转化的转化体能够有效地产生具有PDI活性的多肽的方法。

    Polypeptide from Humicola insolens possessing protein disulfide isomerase activity gene encoding the same
    2.
    发明授权
    Polypeptide from Humicola insolens possessing protein disulfide isomerase activity gene encoding the same 失效
    来自Humicola insolens的多肽具有编码相同的蛋白质二硫键异构酶活性基因

    公开(公告)号：US5496719A

    公开(公告)日：1996-03-05

    申请号：US68395

    申请日：1993-05-27

    申请人： Yukio Yamada ,  Osamu Asami ,  Hidehiko Sugiyama ,  Chie Idekoba ,  Fumihiko Hoshino ,  Masana Hirai ,  Tsutomu Kajino ,  Takao Imaeda ,  Kiyoko Sarai

    发明人： Yukio Yamada ,  Osamu Asami ,  Hidehiko Sugiyama ,  Chie Idekoba ,  Fumihiko Hoshino ,  Masana Hirai ,  Tsutomu Kajino ,  Takao Imaeda ,  Kiyoko Sarai

    IPC分类号： C12N9/90 ,  A61K38/00 ,  C07K1/00 ,  C12N9/00

    CPC分类号： C12N9/90

    摘要： A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polypeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polypeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.

    摘要翻译： 提供了具有蛋白质二硫键异构酶（PDI）活性的高度热稳定的多肽，编码多肽的基因和产生多肽的方法。 具有PDI活性的多肽的特征在于：A）具有催化蛋白质中二硫键交换的能力，B）主要以核糖核酸酶A为底物，C）具有20〜70℃的合适的活性温度，D）为 在pH值为6〜9的条件下稳定，E）分子量约为60,000〜62,000。 由于与常规PDI相比，具有较高的热稳定性并且在更宽的二硫苏糖醇浓度范围内表现出稳定的活性，因此可以提供可有利地用于某些蛋白质的重折叠反应的新型酶活性蛋白质。 此外，还提供了能够使用Humicola insolens或使用含有上述基因的表达载体转化的转化体有效地产生具有PDI活性的多肽的方法。

    Process for production of human growth hormone using Bacillus Brevis
    3.
    发明授权
    Process for production of human growth hormone using Bacillus Brevis 失效
    使用芽孢杆菌生产人生长激素的方法

    公开(公告)号：US5714346A

    公开(公告)日：1998-02-03

    申请号：US507313

    申请日：1995-08-22

    申请人： Shigezo Udaka ,  Tsutomu Kajino ,  Yoko Saito ,  Masana Hirai ,  Yukio Yamada ,  Fumihiko Hoshino

    发明人： Shigezo Udaka ,  Tsutomu Kajino ,  Yoko Saito ,  Masana Hirai ,  Yukio Yamada ,  Fumihiko Hoshino

    IPC分类号： C07K14/61 ,  C12N1/21 ,  C12N15/75 ,  C12P21/06 ,  C07H21/04 ,  C12N1/20

    CPC分类号： C07K14/61 ,  C12N15/75 ,  C07K2319/02

    摘要： A host-vector system suitable for production of human growth hormone (hGH) and a process for production of hGH using the same are provided. As an hGH expression plasmid, a recombinant DNA wherein a DNA coding for hGH is linked to the 3'-terminal of a DNA containing a promoter region derived from Bacillus brevis is provided, and as a host, especially a mutant Bacillus brevis substantially not exhibiting protease activity to hGH is provided. A microorganism obtained by transforming said host with said hGH expression plasmid efficiently produces hGH when it is cultured.

    摘要翻译： PCT No.PCT / JP94 / 00269 Sec。 371日期：1995年8月22日 102（e）日期1995年8月22日PCT 1994年2月22日PCT PCT。 WO94 / 19474 PCT公开号 日期1995年9月1日提供适用于生产人生长激素（hGH）的宿主载体系统和使用其的生产hGH的方法。 作为hGH表达质粒，提供了重组DNA，其中编码hGH的DNA与含有源自短芽孢杆菌的启动子区的DNA的3'-末端连接，作为宿主，特别是基本上不显示的突变体短芽孢杆菌 提供了对hGH的蛋白酶活性。 通过用所述hGH表达质粒转化所述宿主获得的微生物在培养时有效地产生hGH。

    Methods for detection of mutagens using luminescence gene
    6.
    发明授权
    Methods for detection of mutagens using luminescence gene 失效
    使用发光基因检测诱变剂的方法

    公开(公告)号：US5702883A

    公开(公告)日：1997-12-30

    申请号：US326949

    申请日：1994-10-21

    申请人： Takao Imaeda ,  Masana Hirai

    发明人： Takao Imaeda ,  Masana Hirai

    IPC分类号： C07K14/47 ,  C12N15/53 ,  C12Q1/02 ,  C12Q1/68 ,  C07H21/04 ,  C12Q1/66

    CPC分类号： C07K14/47 ,  C12Q1/025 ,  C12Q1/6897

    摘要： A method for detecting or quantitating a mutagenic substance in a sample includes culturing a host microorganism transformed with a recombinant gene comprising an SOS gene and genes expressing luciferase activity and optionally genes expressing an enzyme which catalyzes the production of a substrate for luciferase, positioned downstream of the SOS gene, in a medium to which the sample is added; and measuring a luminescence generated by expression of the gene expressing luciferase activity. The method is sensitive, accurate and non-time consuming; and gene systems used for said method, i.e., a recombinant gene comprising an SOS gene expressed when a DNA is damaged and a gene expressing luciferase activity positioned downstream of the SOS gene, and a host microorganism transformed with said recombinant gene. Preferably the recombinant gene further comprises a gene expressing an enzyme which catalyses the production of a substrate for the luciferase in the down stream of the SOS gene.

    摘要翻译： 用于检测或定量样品中的致突变物质的方法包括培养用包含SOS基因的重组基因和表达荧光素酶活性的基因转化的宿主微生物，以及任选地表达催化产生荧光素酶底物的酶的基因，所述基因位于下游 在添加样品的培养基中的SOS基因; 并测定通过表达荧光素酶活性的基因的表达产生的发光。 该方法灵敏，准确，无耗时; 以及用于所述方法的基因系统，即包含DNA受损时表达的SOS基因的重组基因和位于SOS基因下游的表达荧光素酶活性的基因以及用所述重组基因转化的宿主微生物。 优选地，重组基因还包含表达在SOS基因的下游中催化萤光素酶底物的产生的基因的基因。

    Gas separating members and a method of making the same
    7.
    发明授权
    Gas separating members and a method of making the same 失效
    气体分离构件及其制造方法

    公开(公告)号：US4410338A

    公开(公告)日：1983-10-18

    申请号：US388577

    申请日：1982-06-15

    申请人： Minoru Yamamoto ,  Masana Hirai ,  Jiro Sakata

    发明人： Minoru Yamamoto ,  Masana Hirai ,  Jiro Sakata

    IPC分类号： B01D53/22 ,  B01D57/00 ,  B01D67/00 ,  B01D69/02 ,  B01D71/70 ,  C08G77/06

    CPC分类号： B01D69/127 ,  C08G77/06

    摘要： A gas separating member of the present invention comprises a porous substrate in the form of a film, wall or hollow fiber and a polymer film formed on a surface of the substrate by plasma polymerization. The gas separation factor (O.sub.2 /N.sub.2) ranges from 2.3 to 3.9 with the corresponding gas permeability ranging from 12 to 0.16 liter/min. m.sup.2 atm. pres.-air.A modified gas separating member of the present invention, which comprises a porous substrate in the aforementioned form and two polymer films formed on a surface of the substrate by plasma polymerization, has the gas separation factor (He/H.sub.2) ranging from 14 to 45.

    摘要翻译： 本发明的气体分离部件包括膜，壁或中空纤维形式的多孔基材和通过等离子体聚合在基材的表面上形成的聚合物膜。 气体分离因子（O 2 / N 2）为2.3至3.9，相应的气体渗透率为12至0.16升/分钟。 m2 atm。 空气。 本发明的改性气体分离部件的气体分离系数（He / H 2）为14〜45，包括上述形式的多孔基材和通过等离子体聚合形成于基材表面的2个聚合物膜。