利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

13 条记录 (耗时 0.074 秒)

    G protein coupled receptor protein production, and use thereof
    9.
    发明授权
    G protein coupled receptor protein production, and use thereof 失效
    G蛋白偶联受体蛋白质的产生及其用途

    公开(公告)号：US06538107B1

    公开(公告)日：2003-03-25

    申请号：US09461436

    申请日：1999-12-14

    申请人： Shuji Hinuma ,  Yasuaki Ito ,  Ryo Fujii

    发明人： Shuji Hinuma ,  Yasuaki Ito ,  Ryo Fujii

    IPC分类号： C07K14435

    CPC分类号： C12Q1/6876 ,  C07K14/705 ,  C07K14/70571 ,  C07K14/723 ,  C12Q1/6809 ,  C12Q1/6883

    摘要： DNA primers effective in screening G protein coupled receptor protein-encoding DNA fragments are provided. The primers which are complementary to nucleotide sequences that are in community with (homologous to) the nucleotide sequences encoding amino acid sequences corresponding to or near the first membrane-spanning domain or the sixth membrane-spanning domain each of known various G protein coupled receptor proteins were designed and synthesized. Methods of amplifying G protein coupled receptor protein-encoding DNAs using the above DNA primers, and novel target G protein coupled receptor protein-encoding DNAs are also provided. Screening of DNA libraries can be efficiently carried out. Human pituitary gland or amygdala-derived and mouse pancreas-derived G protein coupled receptor proteins, etc. or salts thereof, partial peptides thereof, DNAs coding for the above G protein coupled receptor proteins, processes for producing the above G protein coupled receptor proteins, methods of determining ligands for the above G protein coupled receptor proteins, methods of screening compounds that inhibit the binding between the ligand and the G protein coupled receptor proteins or screening kits therefor, compounds or salts thereof obtained by the above screening method or the screening kit, pharmaceutical compositions containing the above compounds or salts thereof, and antibodies against the above protein coupled receptor proteins or partial peptides thereof are provided.

    摘要翻译： 提供了有效筛选编码G蛋白偶联受体蛋白的DNA片段的DNA引物。 与与编码对应于或接近第一跨膜结构域或第六跨膜结构域的氨基酸序列的核苷酸序列（同源的）的核苷酸序列互补的引物，每个已知的各种G蛋白偶联受体蛋白 被设计和合成。 还提供了使用上述DNA引物扩增G蛋白偶联受体蛋白质编码DNA的方法，以及新的靶向G蛋白偶联受体蛋白质编码DNA。 可以有效地进行DNA文库的筛选。 人类垂体腺或来自扁桃体的小鼠胰腺衍生的G蛋白偶联受体蛋白等，或其盐，其部分肽，编码上述G蛋白偶联受体蛋白质的DNA，用于产生上述G蛋白偶联受体蛋白质的方法， 确定上述G蛋白偶联受体蛋白质的配体的方法，筛选抑制配体和G蛋白偶联受体蛋白质或其筛选试剂盒之间的结合的化合物的方法，通过上述筛选方法获得的化合物或其盐或筛选试剂盒 提供含有上述化合物或其盐的药物组合物和针对上述蛋白质偶联受体蛋白质或其部分肽的抗体。

    Screening method
    10.
    发明授权
    Screening method 失效
    筛选方法

    公开(公告)号：US07892755B2

    公开(公告)日：2011-02-22

    申请号：US11661555

    申请日：2005-08-29

    申请人： Yasuaki Ito ,  Kazunori Nishi ,  Shoichi Ohkubo ,  Masataka Harada

    发明人： Yasuaki Ito ,  Kazunori Nishi ,  Shoichi Ohkubo ,  Masataka Harada

    IPC分类号： G01N33/566 ,  A61K38/00

    CPC分类号： G01N33/6893 ,  C07K14/705 ,  G01N33/574 ,  G01N2500/00

    摘要： The present invention provides a method of screening a compound or its salt that alters binding properties of a protein comprising the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 1, or a salt thereof, and a ligand capable of specifically binding to the protein or its salt, which comprises using (a) the protein, its partial peptide, or a salt thereof and (b) the ligand; a screening kit therefor, and so on. The screening method and kit of the present invention are useful for screening an agent for the prevention/treatment of, e.g., digestive tract disorders, cancer, immune disorders, type II diabetes mellitus or obesity, etc.

    摘要翻译： 本发明提供一种筛选化合物或其盐的方法，其改变包含与SEQ ID NO：1所示的氨基酸序列相同或基本上相同的氨基酸序列的蛋白质的结合性，或其盐，和 能够特异性结合蛋白质或其盐的配体，其包括使用（a）蛋白质，其部分肽或其盐和（b）配体; 一个筛选工具，等等。 本发明的筛选方法和试剂盒可用于筛选用于预防/治疗例如消化道疾病，癌症，免疫疾病，II型糖尿病或肥胖症等的药剂。