公开(公告)号：US5286484A

公开(公告)日：1994-02-15

申请号：US754918

申请日：1991-09-05

申请人： Silva Rodriquez ,  Selman H. Sosa ,  Guillen Nieto ,  Saturnino H. Martinez ,  Julio R. F. Maso ,  Lidia I. N. Perez ,  Juan M. Grillo ,  Vivian M. Cordova ,  Sonia G. Blanco ,  Beatriz T. Santos ,  Jesus A. del Valle Rosales ,  Evelin C. Menendez ,  Anabel A. Acosta ,  Edelgis C. Rodriquez ,  Silian C. Leon ,  Alexis M. Lasa

发明人： Silva Rodriquez ,  Selman H. Sosa ,  Guillen Nieto ,  Saturnino H. Martinez ,  Julio R. F. Maso ,  Lidia I. N. Perez ,  Juan M. Grillo ,  Vivian M. Cordova ,  Sonia G. Blanco ,  Beatriz T. Santos ,  Jesus A. del Valle Rosales ,  Evelin C. Menendez ,  Anabel A. Acosta ,  Edelgis C. Rodriquez ,  Silian C. Leon ,  Alexis M. Lasa

IPC分类号： A61K39/095 ,  A61K39/00 ,  A61K39/395 ,  A61P31/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/22 ,  C07K14/41 ,  C07K16/00 ,  C07K16/12 ,  C07K16/40 ,  C07K19/00 ,  C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/31 ,  C12N15/53 ,  C12N15/54 ,  C12N15/70 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19 ,  A61K39/02 ,  C07H15/12 ,  C12P21/06

CPC分类号： C12Y108/01004 ,  C07K14/22 ,  C07K16/1217 ,  C07K16/40 ,  C12N9/0051 ,  C12N9/1029 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/035 ,  C07K2319/40

摘要： The present invention is concerned with a method for the isolation of a nucleotide sequence which codes for a protein having a molecular weight of about 64,000 daltons, which is located on the outer membrane of N. meningitidis, as well as with the recombinant DNA obtained therefrom, which is used for the transformation of a host microorganism. The technical object pursued with the invention is the identification of a nucleotide sequence coding for a highly conserved and common protein for the majority of pathogenic Neisseria strains, the production of this protein with a high level of purity and in commercially useful amounts using the recombinant way, so that it can be used in diagnostic methods and vaccine preparations with a broad immunoprotection spectrum.

摘要翻译： 本发明涉及一种分离编码分子量约64,000道尔顿的蛋白质的核苷酸序列的方法，其位于脑膜炎奈瑟氏球菌的外膜上，以及由其获得的重组DNA ，其用于宿主微生物的转化。 本发明追求的技术目标是鉴定编码大多数致病性奈瑟氏球菌菌株的高度保守和常见蛋白质的核苷酸序列，使用重组方法产生具有高水平纯度和商业上有用量的该蛋白质 ，因此可用于具有广泛的免疫保护谱的诊断方法和疫苗制剂中。

公开(公告)号：US5637491A

公开(公告)日：1997-06-10

申请号：US354618

申请日：1994-12-13

申请人： Hernan R. Campana ,  Bianca M. Garcia Garcia ,  Julio M. Delgado Boada ,  Jose A. Cremata Alvarez ,  Maria E. Gonzalez Martinez ,  Emilio M. Clark ,  Dania M. Curbelo ,  Luis S. Herrera Martinez ,  Manuel R. Raices Perez-Castaneda ,  Efrain R. Jiminez ,  Rossana G. Fernandez ,  Vivian M. Cordova ,  Carlos F. Patron

发明人： Hernan R. Campana ,  Bianca M. Garcia Garcia ,  Julio M. Delgado Boada ,  Jose A. Cremata Alvarez ,  Maria E. Gonzalez Martinez ,  Emilio M. Clark ,  Dania M. Curbelo ,  Luis S. Herrera Martinez ,  Manuel R. Raices Perez-Castaneda ,  Efrain R. Jiminez ,  Rossana G. Fernandez ,  Vivian M. Cordova ,  Carlos F. Patron

IPC分类号： C12N9/46 ,  C12N15/55 ,  C12N9/44

CPC分类号： C12N9/2454

摘要： A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production.

摘要翻译： 分离和表达编码真菌青霉的葡聚糖酶的基因的方法。 这种真菌来源的酶是通过在酵母中高水平表达来产生的。 为此目的，分离编码微小霉菌的葡聚糖酶的mRNA的cDNA拷贝并测序。 将该cDNA转入毕赤酵母细胞。 由此获得能够将葡聚糖酶分泌到培养基中的重组酵母。 获得的葡聚糖酶可以使用，例如 在糖业中水解甘蔗汁中的葡聚糖以增加糖的产量。