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公开(公告)号：US20050003436A1
公开(公告)日：2005-01-06
申请号：US10909331
申请日：2004-08-03
IPC分类号： C12N15/09 , C07H21/04 , C12N1/06 , C12N1/08 , C12N1/16 , C12N1/20 , C12N5/00 , C12N13/00 , C12N15/10 , C12P13/00 , C12P19/34 , C12Q1/68 , C12Q1/6806 , C12Q1/6844 , G01F1/64
CPC分类号： C12N13/00 , C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6844 , Y10T436/143333 , C12Q2565/607 , C12Q2531/119 , C12Q2523/307 , C12Q2531/137
摘要： Intracellular material is released from bacterial, yeast, plant, animal, insect or human cells by the application of a low voltage such as 1 to 10 V to a suspension containing the cells. The conditions may be selected such that DNA released from the cells is electrochemically denatured so as to be available for use in an amplification procedure.
摘要翻译： 通过向包含细胞的悬浮液施加1至10V的低电压，将细胞内的细菌，酵母，植物，动物，昆虫或人细胞释放。 可以选择条件使得从细胞释放的DNA被电化学变性，以便可用于扩增过程。
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2.发明授权公开(公告)号：US06335161B1
公开(公告)日：2002-01-01
申请号：US09030028
申请日：1998-02-25
IPC分类号： C12Q168
CPC分类号： C12N13/00 , C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6844 , Y10T436/143333 , C12Q2565/607 , C12Q2531/119 , C12Q2523/307 , C12Q2531/137
摘要： Intracellular material is released from bacterial, yeast, plant, animal, insect or human cells by the application of a low voltage such as 1 to 10 V to a suspension containing the cells. The conditions may be selected such that DNA released from the cells is electrochemically denatured so as to be available for use in an amplification procedure.
摘要翻译： 通过向包含细胞的悬浮液施加1至10V的低电压，将细胞内的细菌，酵母，植物，动物，昆虫或人细胞释放。 可以选择条件使得从细胞释放的DNA被电化学变性，以便可用于扩增过程。
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公开(公告)号：US06333157B1
公开(公告)日：2001-12-25
申请号：US09381896
申请日：2000-03-31
IPC分类号： C12Q168
CPC分类号： C12N15/10 , C12Q1/6813 , Y10T436/143333 , C12Q2527/125
摘要： dsDNA or other interacting molecules, e.g. antibody and antigen, are disassociated by applying an electrical voltage to a solution containing said molecules in a buffer favoring said disassociation e.g. CHES, CAPS OR CAPSO.
摘要翻译： dsDNA或其他相互作用的分子，例如 抗体和抗原通过在缓冲液中施加电压给含有所述分子的溶液来解离，有利于所述分离。 CHES，CAPS或CAPSO。
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公开(公告)号：US06811981B2
公开(公告)日：2004-11-02
申请号：US09994657
申请日：2001-11-28
IPC分类号： C12Q168
CPC分类号： C12N13/00 , C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6844 , Y10T436/143333 , C12Q2565/607 , C12Q2531/119 , C12Q2523/307 , C12Q2531/137
摘要： Intracellular material is released from bacterial, yeast, plant, animal, insect or human cells by the application of a low voltage such as 1 to 10 V to a suspension containing the cells. The conditions may be selected such that DNA released from the cells is electrochemically denatured so as to be available for use in an amplification procedure.
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公开(公告)号：US5744094A
公开(公告)日：1998-04-28
申请号：US122599
申请日：1994-02-24
申请人： Helge Bakketun Castberg , Karin Bergmann , Peter John Hyde , Karen Margaret Montgomery Ness , Christopher John Stanley
发明人： Helge Bakketun Castberg , Karin Bergmann , Peter John Hyde , Karen Margaret Montgomery Ness , Christopher John Stanley
CPC分类号： A61L2/10
摘要： A sterilizing method includes subjecting material, for example packaging board, to laser UV alone, or substantially simultaneously with laser IR and/or hydrogen peroxide to obtain a synergistic effect between the UV and IR and/or hydrogen peroxide, to render micro-organisms present at said material non-viable.
摘要翻译： PCT No.PCT / GB92 / 00671 Sec。 371日期1994年2月24日 102（e）1994年2月24日PCT PCT 1992年4月13日提交PCT公布。 公开号WO92 / 18170 日期：1992年10月29日消毒方法包括将材料（例如包装板）单独激光UV或与激光IR和/或过氧化氢基本上同时地进行激光UV以获得UV和IR和/或过氧化氢之间的协同效应， 使存在于所述材料上的微生物不能存活。
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