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    PROCESS FOR REACTING A CARBOXYLIC ACID ESTER
    1.
    发明申请
    PROCESS FOR REACTING A CARBOXYLIC ACID ESTER 审中-公开
    反应羧酸酯的方法

    公开(公告)号：US20140141478A1

    公开(公告)日：2014-05-22

    申请号：US14077750

    申请日：2013-11-12

    申请人： Steffen SCHAFFER ,  Heiko Andrea ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger

    发明人： Steffen SCHAFFER ,  Heiko Andrea ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger

    IPC分类号： C12P7/62

    摘要： The invention provides a process for reacting a carboxylic acid ester of the formula (I) R1-A-COOR2   (I), wherein R1 is hydrogen, —CH2OH, —CHO, —COOR3, —CH2SH, —CH2OR3 or —CH2NH2, R2 is an alkyl group,R3 is hydrogen or an alkyl group, and A is a substituted, unsubstituted, linear, branched and/or cyclic alkylene, alkenylene, arylene or aralkylene radical having at least 4 carbons,in the presence of a cell. The process comprises a) contacting the cell with said carboxylic acid ester in an aqueous solution,wherein the cell is a recombinant cell which has reduced activity of a polypeptide comprising SEQ ID NO: 2 or a variant thereof over the wild-type cell.

    摘要翻译： 本发明提供使式（I）R1-A-COOR2（Ⅰ）的羧酸酯与式（Ⅰ）中R1为氢，-CH2OH，-CHO，-COOR3，-CH2SH，-CH2OR3或-CH2NH2，R2 在烷基存在下，R 3是氢或烷基，A是具有至少4个碳的取代的，未被取代的，直链的，支链的和/或环状的亚烷基，亚烯基，亚芳基或亚芳基。 该方法包括a）使细胞与所述羧酸酯在水溶液中接触，其中所述细胞是包含SEQ ID NO：2或其变体的多肽在野生型细胞上的活性降低的重组细胞。

    PRODUCTION OF OMEGA-AMINO FATTY ACIDS
    6.
    发明申请
    PRODUCTION OF OMEGA-AMINO FATTY ACIDS 有权
    欧米茄 - 脂肪酸的生产

    公开(公告)号：US20140178948A1

    公开(公告)日：2014-06-26

    申请号：US14132473

    申请日：2013-12-18

    申请人： Steffen SCHAFFER ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

    发明人： Steffen SCHAFFER ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

    IPC分类号： C12P13/00

    CPC分类号： C12P13/001 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/0069 ,  C12N9/1096 ,  C12N9/1288 ,  C12P13/005 ,  Y02P20/582

    摘要： The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

    摘要翻译： 本发明提供一种全细胞催化剂，其表达重组α-加氧酶或重组脂肪酸还原酶与磷酸巯基乙酰转移酶的磷酸聚糖乙酰化脂肪酸还原酶的组合，除了α-加氧酶和/或脂肪酸的组合 还原酶和磷酸泛酰乙酰转移酶表达转氨酶，其特征在于磷酸泛酰乙酰转移酶和/或转氨酶优选是重组的; 以及将脂肪酸ω-羟基脂肪酸，ω-氧代脂肪酸或其单酯转化成胺的方法，包括脂肪酸，ω-羟基脂肪酸，ω-氧代脂肪酸或 其单酯通过与烷烃羟化酶和/或醇脱氢酶接触而与氧化产物接触，使氧化产物与磷酸泛酰乙酰化的脂肪酸还原酶或α-双加氧酶接触，得到醛，并使醛与转氨酶接触。