Method of purifying RNA binding protein-RNA complexes
    2.
    发明申请
    Method of purifying RNA binding protein-RNA complexes 审中-公开
    RNA结合蛋白RNA复合物的纯化方法

    公开(公告)号:US20050227251A1

    公开(公告)日:2005-10-13

    申请号:US10971736

    申请日:2004-10-25

    IPC分类号: C12N15/10 C12P19/34 C12Q1/68

    摘要: The present invention provides methods for purifying RNA molecules interacting with an RNA binding protein (RBP), and the use of such methods to analyze a gene expression profile of a cell. The invention also provides sequences of RNA molecules that mediate binding to an RBP, proteins encoded by the sequences, a method of identifying the sequences, and the use of the sequences in a screen to identify bioactive molecules. The invention also provides RNA motifs found among the sequences and compounds that bind the RNA motifs. In addition, the invention provides methods of treating diseases associated with a function of an RNA binding protein.

    摘要翻译: 本发明提供了用于纯化与RNA结合蛋白(RBP)相互作用的RNA分子的方法,以及使用这些方法来分析细胞的基因表达谱。 本发明还提供介导与RBP结合的RNA分子的序列,由序列编码的蛋白质,鉴定序列的方法以及筛选中序列用于鉴定生物活性分子的序列。 本发明还提供在结合RNA基序的序列和化合物中发现的RNA基序。 此外,本发明提供了治疗与RNA结合蛋白的功能相关的疾病的方法。

    Method of Purifying RNA Binding Protein-RNA Complexes
    3.
    发明申请
    Method of Purifying RNA Binding Protein-RNA Complexes 审中-公开
    纯化RNA结合蛋白-RNA复合物的方法

    公开(公告)号:US20110076676A1

    公开(公告)日:2011-03-31

    申请号:US12509856

    申请日:2009-07-27

    IPC分类号: C12Q1/68

    摘要: The present invention provides methods for purifying RNA molecules interacting with an RNA binding protein (RBP), and the use of such methods to analyze a gene expression profile of a cell. The invention also provides sequences of RNA molecules that mediate binding to an RBP, proteins encoded by the sequences, a method of identifying the sequences, and the use of the sequences in a screen to identify bioactive molecules. The invention also provides RNA motifs found among the sequences and compounds that bind the RNA motifs. In addition, the invention provides methods of treating diseases associated with a function of an RNA binding protein.

    摘要翻译: 本发明提供了用于纯化与RNA结合蛋白(RBP)相互作用的RNA分子的方法,以及使用这些方法来分析细胞的基因表达谱。 本发明还提供介导与RBP结合的RNA分子的序列,由序列编码的蛋白质,鉴定序列的方法以及筛选中序列用于鉴定生物活性分子的序列。 本发明还提供在结合RNA基序的序列和化合物中发现的RNA基序。 此外,本发明提供了治疗与RNA结合蛋白的功能相关的疾病的方法。