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53 条记录 (耗时 0.027 秒)

    2,3-disubstituted-1,3,2-oxazaphosphacycloalkanes as nucleic acid linking agents
    1.
    发明授权
    2,3-disubstituted-1,3,2-oxazaphosphacycloalkanes as nucleic acid linking agents 失效
    2,3-二取代-1,3,2-氧杂氮杂环烷烃作为核酸连接剂

    公开(公告)号：US5258538A

    公开(公告)日：1993-11-02

    申请号：US734575

    申请日：1991-10-29

    申请人： Steven Fung ,  Sam L. Woo ,  Lloyd M. Smith

    发明人： Steven Fung ,  Sam L. Woo ,  Lloyd M. Smith

    IPC分类号： A61K47/48 ,  C07F9/24 ,  C07F9/6584 ,  C07H21/00 ,  C07F9/00

    CPC分类号： C07H21/00 ,  A61K47/48023 ,  A61K47/48061 ,  A61K47/48084 ,  C07F9/2408 ,  C07F9/65842

    摘要： The compounds of the invention include novel linking agents comprising 2-substituted-3-protected-1,3,2-oxazaphosphacycloalkanes and their phosphoramidite precursors. The compounds of the invention further include conjugates of the above linking agents with oligonucleotides and polymer supports. The compounds of the present invention are useful for linking organic moieties, such as fluorescent or chromogenic dyes, to polymer supports and oligonucleotides, particularly single- and double-stranded DNA and RNA fragments.

    摘要翻译： 本发明的化合物包括包含2-取代-3-保护的1,3,2-氧杂氮杂环烷烃及其亚磷酰胺前体的新型交联剂。 本发明的化合物还包括上述连接剂与寡核苷酸和聚合物载体的缀合物。 本发明的化合物可用于将有机部分如荧光或显色染料与聚合物载体和寡核苷酸，特别是单链和双链DNA和RNA片段连接。

    Method of DNA sequencing employing a mixed DNA-polymer chain probe
    4.
    发明授权
    Method of DNA sequencing employing a mixed DNA-polymer chain probe 失效
    使用混合DNA-聚合物链探针进行DNA测序的方法

    公开(公告)号：US5624800A

    公开(公告)日：1997-04-29

    申请号：US447174

    申请日：1995-05-19

    申请人： Paul D. Grossman ,  Steven Fung ,  Steven M. Menchen ,  Sam L. Woo ,  Emily S. Winn-Deen

    发明人： Paul D. Grossman ,  Steven Fung ,  Steven M. Menchen ,  Sam L. Woo ,  Emily S. Winn-Deen

    IPC分类号： C12N15/09 ,  B01D57/02 ,  C12Q1/68 ,  G01N27/447 ,  G01N30/88 ,  G01N33/566 ,  C12P19/34

    CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6818 ,  Y10T436/143333

    摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.

    摘要翻译： 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。 所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。 标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

    Solid support reagents for the synthesis of 3'-Nitrogen containing polynucleotides
    5.
    发明授权
    Solid support reagents for the synthesis of 3'-Nitrogen containing polynucleotides 失效
    用于合成含3'-氮的多核苷酸的固体支持试剂

    公开(公告)号：US5552471A

    公开(公告)日：1996-09-03

    申请号：US293637

    申请日：1994-08-17

    申请人： Sam L. Woo ,  Steven Fung

    发明人： Sam L. Woo ,  Steven Fung

    IPC分类号： C12N15/09 ,  C07C217/20 ,  C07C271/10 ,  C07C271/16 ,  C07C271/66 ,  C07C317/18 ,  C07C333/04 ,  C07H21/00 ,  C07H21/04 ,  C03C12/00 ,  C08L25/06

    CPC分类号： C07H21/00 ,  Y02P20/55

    摘要： The compounds are exemplified by the class of sulfoethyl oxygen-substituted carbamates, such compounds being useful as support reagents for automated polynucleotide synthesis of 3'-nitrogen functionalized polynucleotides. The invention includes, in one aspect, a polynucleotide synthesis reagent having the structure: ##STR1## where T is an acid-cleavable hydroxyl protecting group, e.g., 4,4'-dimethoxytritil; Q is a linker connecting the nitrogen and oxygen, e.g., n-hexyl; R.sub.1 is a nitrogen substituent, e.g., hydrogen; R.sub.2 through R.sub.4 are separately hydrogen or lower alkyl; Y is an atom which is electronegative with respect to carbon, e.g., oxygen or sulfur; X.sub.1 is an atom which is electronegative with respect to carbon, e.g., sulfone; Z is a bond or spacer arm, e.g., ethylsuccinate; and W is a derivatized solid synthesis support capable of linking to Z, e.g., an amino-dirivitized controlled pore glass. In a second aspect, the present invention includes a polynucleotide synthesis reagent having the structure: ##STR2## where T, Q, R.sub.1, R.sub.2 -R.sub.3, X.sub.2, Z, and W are as defined above.

    摘要翻译： 所述化合物的例子是磺乙氧基取代的氨基甲酸酯类，这些化合物可用作3'-氮官能化多核苷酸的自动多核苷酸合成的载体试剂。 在一个方面，本发明包括具有以下结构的多核苷酸合成试剂：其中T是酸可裂解的羟基保护基，例如4,4'-二甲氧基苦味; Q是连接氮和氧的接头，例如正己基; R1是氮取代基，例如氢; R2至R4分别为氢或低级烷基; Y是相对于碳例如氧或硫具有电负性的原子; X1是相对于碳电负性的原子，例如砜; Z是键或间隔臂，例如乙基琥珀酸盐; 并且W是能够与Z连接的衍生的固体合成载体，例如氨基 - 二硝基化的受控孔玻璃。 在第二方面，本发明包括具有以下结构的多核苷酸合成试剂：其中T，Q，R 1，R 2，R 3，X 2，Z和W如上所定义。

    Method and probe composition for detecting multiple sequences in a single assay
    6.
    发明授权
    Method and probe composition for detecting multiple sequences in a single assay 失效
    用于在单个测定中检测多个序列的方法和探针组合物

    公开(公告)号：US5514543A

    公开(公告)日：1996-05-07

    申请号：US102372

    申请日：1993-08-04

    申请人： Paul D. Grossman ,  Steven Fung ,  Steven M. Menchen ,  Sam L. Woo ,  Emily S. Win-Deen

    发明人： Paul D. Grossman ,  Steven Fung ,  Steven M. Menchen ,  Sam L. Woo ,  Emily S. Win-Deen

    IPC分类号： C12N15/09 ,  B01D57/02 ,  C12Q1/68 ,  G01N27/447 ,  G01N30/88 ,  G01N33/566

    CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6818 ,  Y10T436/143333

    摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.

    摘要翻译： 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在本发明的一个实施方案中，将多个不同序列探针对加入到靶多核苷酸中，其中每个探针对包含两个多核苷酸探针元件，该多核苷酸探针元件与目标中所选择的一个目标序列的相邻部分互补 多核苷酸。 在每个探针对中，探针元件中的一个包含非多核苷酸聚合物链，当对中的元件被连接时，赋予相关探针对的特异性移动性。 该对中的另一个元素包含可检测的报告物标记。 在探针对已被允许与靶多核苷酸杂交后，杂交的多核苷酸在有效连接靶结合探针元件的末端亚基的条件下处理，当它们的末端亚基与相邻的靶基碱基配对时。 然后将连接的探针对从目标多核苷酸中释放，并在筛分基质中电泳分离，或进行色谱分离。