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    Oligonucleotides and methods for inhibiting cellular proliferation
    1.
    发明授权
    Oligonucleotides and methods for inhibiting cellular proliferation 失效
    寡核苷酸和抑制细胞增殖的方法

    公开(公告)号：US5945336A

    公开(公告)日：1999-08-31

    申请号：US975570

    申请日：1997-11-21

    申请人： Steven Joel Brown ,  Nanibhushan Dattagupta ,  Yathi M. Naidu

    发明人： Steven Joel Brown ,  Nanibhushan Dattagupta ,  Yathi M. Naidu

    IPC分类号： A61K38/00 ,  C12N15/113 ,  C07H21/00 ,  C12N5/06

    CPC分类号： C12N15/1138 ,  A61K38/00 ,  C12N2310/315

    摘要： The present invention relates to methods of treating disease-associated cellular proliferation using oligonucleotides. In particular, it relates to the use of oligonulceotides which are substantially complementary to interleukin-6 receptor mRNA sequences. In the form of pharmaceutical compositions, these oligonucleotides are suitable for administration to human subjects for the treatment of abnormal cellular proliferation due to such diseases as cancer, autoimmune disorders and viral infection.

    摘要翻译： 本发明涉及使用寡核苷酸治疗疾病相关细胞增殖的方法。 特别地，它涉及与白细胞介素-6受体mRNA序列基本上互补的寡核苷酸的用途。 以药物组合物的形式，这些寡核苷酸适合于给予受试者治疗由于诸如癌症，自身免疫病症和病毒感染等疾病引起的异常细胞增殖。

    Compositions and methods for detecting pathogenic bacteria expressing chaperonin proteins
    6.
    发明申请
    Compositions and methods for detecting pathogenic bacteria expressing chaperonin proteins 审中-公开
    用于检测表达伴侣蛋白的致病菌的组合物和方法

    公开(公告)号：US20060073475A1

    公开(公告)日：2006-04-06

    申请号：US10216488

    申请日：2002-08-09

    申请人： Nanibhushan Dattagupta ,  Ketan Shah

    发明人： Nanibhushan Dattagupta ,  Ketan Shah

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/689 ,  C12Q1/6837

    摘要： This invention relates generally to compositions and methods for detection of pathogenic bacteria that express extracellular chaperonin proteins. In one embodiment, it relates to Mycobacterium tuberculosis (Mtb) detection and provides for novel probes for a specific and sensitive diagnostic test for Mycobacterium tuberculosis complex (TBC) that hybridize with the groEL-1 gene. Arrays comprising the novel probes immobilized on a support for hybridization analysis and methods for TBC detection using the probes are also provided.

    摘要翻译： 本发明一般涉及用于检测表达细胞外伴侣蛋白的致病菌的组合物和方法。 在一个实施方案中，其涉及结核分枝杆菌（Mtb）检测，并提供用于与groEL-1基因杂交的结核分枝杆菌复合物（TBC）的特异性和灵敏的诊断测试的新型探针。 还提供了包含固定在用于杂交分析的载体上的新型探针的阵列和使用探针进行TBC检测的方法。

    Isothermal strand displacement nucleic acid amplification
    8.
    发明授权
    Isothermal strand displacement nucleic acid amplification 失效
    等温链置换核酸扩增

    公开(公告)号：US6087133A

    公开(公告)日：2000-07-11

    申请号：US972799

    申请日：1997-11-18

    申请人： Nanibhushan Dattagupta ,  Paul Douglas Stull ,  Marc Spingola ,  Daniel Louis Kacian

    发明人： Nanibhushan Dattagupta ,  Paul Douglas Stull ,  Marc Spingola ,  Daniel Louis Kacian

    IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6844

    摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5' exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.

    摘要翻译： 使用缺少5'核酸外切酶活性的核酸聚合酶和一组寡核苷酸引物扩增靶核酸序列的方法。 优选使用引物数组。 引物数组包含两组引物。 一组含有至少两个互补引物。 另一组包含至少两个有义引物。 使用所述方法可以在基本恒定的环境条件下进行扩增，而不需要外切核酸酶活性或限制性内切核酸酶活性。

    Coumarin-labeled nucleoside 5'-triphosphates
    10.
    发明授权
    Coumarin-labeled nucleoside 5'-triphosphates 失效
    香豆素标记核苷5'-三磷酸

    公开(公告)号：US5587472A

    公开(公告)日：1996-12-24

    申请号：US460027

    申请日：1995-06-02

    申请人： Nanibhushan Dattagupta ,  Jurgen Kocher

    发明人： Nanibhushan Dattagupta ,  Jurgen Kocher

    IPC分类号： C07H21/00 ,  C07H19/10 ,  C07H19/20 ,  C07H21/04

    CPC分类号： C07H21/00

    摘要： A fluorescent label compound having the formulaA--B.sup.1 --B.sup.2 --B.sup.3 --CwhereinA represents the residue of a natural or synthetic nucleotide or nucleoside or a derivative thereof;B.sup.1 represents a divalent spacer radical or a single bond;B.sup.2 represents a divalent spacer radical;B.sup.3 represents a divalent spacer radical or a single bond; andC represents a coumarin radical having the formula ##STR1## in which R.sup.1 represents hydrogen or cyano;R.sup.2 represents phenyl or thiazolyl bonded in the 2-, 4- or 5-position, wherein the phenyl is unsubstituted or substituted by nitro, cyano, amino, --NH--C.sub.1-4 -alkyl, --(CH.sub.2).sub.1-4 --NH.sub.2, --(CH.sub.2).sub.1-4 --NH--(CH.sub.2).sub.1-3 --CH.sub.3, carboxy, C.sub.1-4 -alkoxycarbonyl, C.sub.1-4 -alkoxycarbonyloxy, hydroxy, C.sub.1-4 -alkylaminocarbonyl, or C.sub.1-4 -alkylcarbonylamino and either further unsubstituted or substituted by C.sub.1-4 -alkyl, fluorine, chlorine or bromine, and where the thiazolyl is unsubstituted or monosubstituted or disubstituted by chlorine, cyano, carboxyl, or C.sub.1-4 -alkoxycarbonyl, or the thiazolyl is fused in the 4- and 5-position with a benzene ring which is either unsubstituted or substituted by carboxyl, amino, or hydroxyl;R.sup.3 represents hydrogen, C.sub.1-4 -alkyl, C.sub.1-4 -alkoxycarbonyl-C.sub.1-4 -alkyl, or phenylsulphonyl, where the C.sub.1-4 -alkyl is unsubstituted or substituted by hydroxyl, amino, carboxyl, or C.sub.1-4 -alkoxycarbonyl, and where the phenylsulphonyl is unsubstituted or monosubstituted or disubstituted by chlorine, bromine, or C.sub.1-4 -alkyl;or where one of the radicals R.sup.2 or R.sup.3 denotes or is substituted by a primary or secondary amino group, hydroxyl, carboxyl, or C.sub.1-4 -alkoxycarbonyl, or can be converted into such a group by hydrolysis or hydrogenation.

    摘要翻译： 具有式A-B1-B2-B3-C的荧光标记化合物，其中A表示天然或合成的核苷酸或核苷或其衍生物的残基; B1代表二价间隔基团或单键; B2表示二价间隔基; B3表示二价间隔基或单键; 并且C表示具有式“IMAGE”的香豆素基团，其中R 1表示氢或氰基; 氰基，氨基，-NH-C 1-4 - 烷基， - （CH 2）1-4-NH 2，其中苯基未被取代或被硝基，氰基，氨基， ， - （CH 2）1-4-NH-（CH 2）1-3 -CH 3，羧基，C 1-4 - 烷氧基羰基，C 1-4 - 烷氧基羰基氧基，羟基，C 1-4 - 烷基氨基羰基或C 1-4 - 烷基羰基氨基 进一步未取代或被C 1-4 - 烷基，氟，氯或溴取代，并且其中噻唑基未被取代或被氯，氰基，羧基或C 1-4 - 烷氧基羰基单取代或二取代，或噻唑基稠合在4- 和5-位，其中苯环是未取代的或被羧基，氨基或羟基取代; R 3表示氢，C 1-4 - 烷基，C 1-4 - 烷氧基羰基-C 1-4 - 烷基或苯基磺酰基，其中C 1-4 - 烷基未被取代或被羟基，氨基，羧基或C 1-4 - 烷氧基羰基取代， 并且其中苯基磺酰基未被取代或被氯，溴或C 1-4 - 烷基单取代或二取代; 或其中一个基团R 2或R 3表示或被伯或仲氨基，羟基，羧基或C 1-4 - 烷氧基羰基取代，或者可以通过水解或氢化转化为该基团。