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1.发明授权Methods and compositions for detection of Mycobacterium avium complex species 有权用于检测鸟分枝杆菌复合物种的方法和组合物公开(公告)号：US07361746B2
公开(公告)日：2008-04-22
申请号：US10862026
申请日：2004-06-04
CPC分类号： C12Q1/689
摘要： Methods of detecting Mycobacterium avium complex (MAC) organisms using in vitro nucleic acid amplification with amplification oligonucleotides specific for 16S rRNA or DNA sequences encoding 16S rRNA from MAC species are disclosed. Compositions and kits containing oligonucleotides for amplifying and detecting 16S rRNA or DNA sequences encoding 16S rRNA from MAC species are disclosed.
摘要翻译： 公开了使用具有16S rRNA特异性的扩增寡核苷酸或编码来自MAC物种的16S rRNA的DNA序列的体外核酸扩增来检测鸟分枝杆菌复合物（MAC）生物体的方法。 公开了含有用于扩增和检测来自MAC物种的16S rRNA的16S rRNA或DNA序列的寡核苷酸的组合物和试剂盒。
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2.发明授权Methods and compositions for detection of mycobacterium avium complex species 有权用于检测鸟分枝杆菌复合物种的方法和组合物公开(公告)号：US06747141B2
公开(公告)日：2004-06-08
申请号：US09738972
申请日：2000-12-15
IPC分类号： C12Q168
CPC分类号： C12Q1/689
摘要： Methods of detecting Mycobacterium avium complex (MAC) organisms using in vitro nucleic acid amplification with amplification oligonucleotides specific for 16S rRNA or DNA sequences encoding 16S rRNA from MAC species are disclosed. Compositions and kits containing oligonucleotides for amplifying and detecting 16S rRNA or DNA sequences encoding 16S rRNA from MAC species are disclosed.
摘要翻译： 公开了使用具有16S rRNA特异性的扩增寡核苷酸或编码来自MAC物种的16S rRNA的DNA序列的体外核酸扩增来检测鸟分枝杆菌复合物（MAC）生物体的方法。 公开了含有用于扩增和检测来自MAC物种的16S rRNA的16S rRNA或DNA序列的寡核苷酸的组合物和试剂盒。
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公开(公告)号：US07696337B2
公开(公告)日：2010-04-13
申请号：US11574307
申请日：2005-08-26
申请人： Michael M. Becker , Steven T. Brentano , Daniel P. Kolk , Wai-Chung Lam , Kristin W. Livezey , Norman C. Nelson , Astrid R. W. Schroder , Gary P. Schroth
发明人： Michael M. Becker , Steven T. Brentano , Daniel P. Kolk , Wai-Chung Lam , Kristin W. Livezey , Norman C. Nelson , Astrid R. W. Schroder , Gary P. Schroth
CPC分类号： C12P19/34 , C12Q1/6865 , C12Q2537/163 , C12Q2521/325 , C12Q2521/107 , C12Q2525/186 , C12Q2533/101 , C12Q2525/143
摘要： The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side products. The method uses only one primer, the “priming oligonucleotide,” a 3′blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or eliminating the formation of side products. The method of the present invention minimizes or eliminates the emergence of side products, thus providing a high level of specificity. Furthermore, the appearance of side products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or eliminates this problem, thus providing an enhanced level of sensitivity.
摘要翻译： 本发明涉及合成目标核酸序列的多个拷贝的新型方法，所述目标核酸序列是自动催化的（即，能够自动循环而不需要修饰诸如温度，pH或离子强度的反应条件，并且使用一种 循环在下一个）。 特别地，本发明公开了一种鲁棒有效的核酸扩增方法，同时减少了副产物的外观。 该方法仅使用一种引物，即引物寡核苷酸，3'封闭的启动子寡核苷酸和任选的终止引物延伸反应的手段，以体外扩增RNA或DNA分子，同时减少或消除副产物的形成。 本发明的方法使副产物的出现最小化或消除，从而提供高水平的特异性。 此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。 本发明使这个问题最小化或消除了这一点，从而提供了增强的灵敏度。
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4.发明授权公开(公告)号：US07544792B2
公开(公告)日：2009-06-09
申请号：US11182177
申请日：2005-07-13
CPC分类号： C12Q1/6865 , C12Q1/706 , C12Q2600/158 , C12Q2600/16 , Y02A50/451 , Y02A50/54
摘要： Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.
摘要翻译： 公开了用于检测样品中HAV RNA序列的存在的核酸寡聚序列和体外核酸扩增和检测方法。 公开了包含用于扩增和检测HAV核酸序列的核酸低聚物的试剂盒。
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公开(公告)号：US07326533B2
公开(公告)日：2008-02-05
申请号：US11495262
申请日：2006-07-27
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.
摘要翻译： 一种检测存在于生物样品中的结核分枝杆菌的rpoB序列的方法，其包括在包括特异性公开的引物序列的核酸扩增混合物中体外扩增结核分枝杆菌rpoB序列的步骤，并通过使用提供的探针检测扩增的序列 公开了通过其与部分扩增核酸的特异性杂交的信息。 公开了用于在体外扩增和检测样品中结核分枝杆菌的rpoB序列的组合物。
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公开(公告)号：US07094542B2
公开(公告)日：2006-08-22
申请号：US10245988
申请日：2002-09-18
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences using multiple probes that provide sequence information by their specific hybridization to portions of the amplified nucleic acid. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.
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7.发明授权Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe 有权使用修饰的寡核苷酸探针测定样品中RNA分析物的存在的方法公开(公告)号：US07070925B1
公开(公告)日：2006-07-04
申请号：US09565427
申请日：2000-05-05
CPC分类号： C12Q1/6832 , C07H21/00 , C12Q1/6816 , C12Q1/6844 , C12Q1/686 , C12Q1/6865 , C12Q2563/131 , C12Q2563/113 , C12Q2525/101 , C12Q2525/117 , C12Q2527/107
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。
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公开(公告)号：US06534273B2
公开(公告)日：2003-03-18
申请号：US09956412
申请日：2001-09-18
申请人： William G. Weisburg , Jay H. Shaw , Michael M. Becker , Mehrdad R. Majlessi , Steven T. Brentano , Kiyotada Nunomura
发明人： William G. Weisburg , Jay H. Shaw , Michael M. Becker , Mehrdad R. Majlessi , Steven T. Brentano , Kiyotada Nunomura
IPC分类号： C12Q168
CPC分类号： C12Q1/6834 , C12Q1/6813
摘要： A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions that control the order of hybridization, where the first hybridization condition allows hybridization of the capture probe to the target polynucleotide, and the second hybridization condition allows hybridization of the capture probe to the immobilized probe. The method further includes amplifying the captured target polynucleotide by hybridizing at least one primer oligonucleotide to the target polynucleotide and using nucleic acid amplification that initiates from the primer oligonucleotide.
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公开(公告)号：US06297365B1
公开(公告)日：2001-10-02
申请号：US09365121
申请日：1999-07-30
IPC分类号： C07H2102
CPC分类号： C12Q1/6865 , C07H21/00 , C12Q1/6806 , C12Q2525/301 , C12Q2549/125
摘要： The present invention features inhibitors of target-independent amplification and the use of such inhibitors for enhancing an amplification protocol. The inhibitors are believed to enhance an amplification protocol by inhibiting the ability of one or more nucleic acid polymerases to use nucleic acid in a polymerase reaction in the absence of target nucleic acid.
摘要翻译： 本发明的特征在于靶依赖性扩增的抑制剂以及这种抑制剂用于增强扩增方案的用途。 认为抑制剂通过抑制一种或多种核酸聚合酶在不存在靶核酸的聚合酶反应中使用核酸的能力来增强扩增方案。
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10.发明授权Compositions and methods for detecting the presence of Mycobacterium kansassii 失效用于检测堪萨斯分枝杆菌存在的组合物和方法公开(公告)号：US06218107B1
公开(公告)日：2001-04-17
申请号：US08856963
申请日：1997-05-15
IPC分类号： C07H2104
CPC分类号： C12Q1/689
摘要： The featured invention discloses and claims oligonucleotide hybridization assay probes and helper oligonucleotides which are designed to be complementary to specific regions of M. kansasii rRNA or the DNA encoding it, or to an oligonucleotide or nucleic acid comprising, consisting essentially of, or consisting of, a M. kansasii rRNA or rDNA nucleotide sequence. The hybridization probes of the present invention are designed to hybridize to a target nucleic acid in a region of the molecule having a specific target nucleotide sequence under conditions which allow the selective detection of the target nucleic acid. The probes are further designed to detect M. kansasii typical as well as atypical strains. The present invention also discloses and claims double-stranded nucleic acid hybrid molecules formed between the hybridization probes and their specific target nucleic acids.
摘要翻译： 本发明公开并要求的是寡核苷酸杂交测定探针和辅助寡核苷酸，其被设计为与堪萨斯堪萨斯大学rRNA或编码它的DNA的特定区域互补，或者涉及包含基本上由...组成或由其构成的寡核苷酸或核酸， 堪萨斯大肠杆菌rRNA或rDNA核苷酸序列。本发明的杂交探针被设计为在允许选择性检测靶核酸的条件下与具有特异性靶核苷酸序列的分子区域中的靶核酸杂交 。 探针进一步设计用于检测典型的堪萨斯（Kansasii）典型菌株和非典型菌株。本发明还公开并要求在杂交探针与其特异性靶核酸之间形成的双链核酸杂交分子。
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