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    Methods for Detection of Micro-Organisms
    1.
    发明申请
    Methods for Detection of Micro-Organisms 审中-公开
    微生物检测方法

    公开(公告)号：US20100291564A1

    公开(公告)日：2010-11-18

    申请号：US12667818

    申请日：2008-07-09

    申请人： Christopher John Stanley ,  Stuart Wilson

    发明人： Christopher John Stanley ,  Stuart Wilson

    IPC分类号： C12Q1/68 ,  C12N9/48

    CPC分类号： C12Q1/04 ,  C12N9/93 ,  C12Q1/02 ,  C12Q1/18 ,  C12Q1/25 ,  C12Q1/689 ,  G01N2333/9015 ,  C12Q2565/301 ,  C12Q2521/501

    摘要： NAD-dependent ligase is identified as an indicator of micro-organisms in a sample. A method of detecting the presence of an NAD-dependent ligase expressing micro-organism in a sample comprises the steps of: (a) contacting the sample with a nucleic acid molecule which acts as a substrate for NAD-dependent ligase activity in the sample, (b) incubating the thus contacted sample under conditions suitable for NAD-dependent ligase activity; and (c) specifically determining the presence of a ligated nucleic acid molecule resulting from the action of the NAD-dependent ligase on the substrate nucleic acid molecule to indicate the presence of the NAD-dependent ligase expressing micro-organism. The method has a number of applications and kits for carrying out the methods are also provided. Lysostaphin preparations substantially free from nuclease and/or ligase contaminants are produced by heating a lysostaphin preparation which contains nuclease and/or ligase contaminants under conditions whereby nuclease and/or ligase activity is reduced whereas endopeptidase activity of the lysostaphin is substantially unaffected.

    摘要翻译： NAD依赖性连接酶被鉴定为样品中微生物的指标。 检测样品中NAD依赖性连接酶表达微生物的存在的方法包括以下步骤：（a）使样品与作为样品中NAD依赖性连接酶活性的底物的核酸分子接触， （b）在适合于NAD依赖性连接酶活性的条件下孵育由此接触的样品; 和（c）特异性地确定由NAD依赖性连接酶在底物核酸分子上的作用产生的连接的核酸分子的存在，以指示表达微生物的NAD依赖性连接酶的存在。 该方法还提供了许多用于执行方法的应用和套件。 通过在其中核酸酶和/或连接酶活性降低的条件下加热含有核酸酶和/或连接酶污染物的溶葡萄球菌素制剂而产生基本上不含核酸酶和/或连接酶污染物的溶葡萄球菌素制剂，而溶葡萄球菌素的内肽酶活性基本上不受影响。

    DETECTION OF BACTERIA AND FUNGI
    2.
    发明申请
    DETECTION OF BACTERIA AND FUNGI 有权
    检测细菌和真菌

    公开(公告)号：US20120107865A1

    公开(公告)日：2012-05-03

    申请号：US13264177

    申请日：2010-04-19

    申请人： Stuart Wilson ,  William Mullen

    发明人： Stuart Wilson ,  William Mullen

    IPC分类号： C12Q1/25

    CPC分类号： C12Q1/04 ,  C12Q1/68 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2521/501 ,  C12Q2527/119 ,  C12Q2533/10 ,  C12Q2537/149 ,  G01N2333/9015

    摘要： A method of detecting a ligase expressing micro-organism in a sample comprises steps of treating the sample under conditions that inhibit the activity of ATP-dependent ligase from mammalian cells but which do not inhibit the activity of the microbial ligases, contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for ligase activity in the sample, incubating the thus contacted sample under conditions suitable for ligase activity; and specifically determining the presence and/or the amount of a ligated nucleic acid molecule resulting from the action of the ligase on the substrate nucleic acid molecule to indicate the presence of the ligase expressing micro-organism. The micro-organism may be a fungus or a bacterium or both. High pH conditions may be employed to inactivate mammalian ligases. Related kits are described.

    摘要翻译： 检测样品中连接酶表达微生物的方法包括以下步骤：在抑制ATP依赖性连接酶从哺乳动物细胞的活性但不抑制微生物连接酶的活性，接触样品或 样品的一部分与核酸分子起作用，作为样品中连接酶活性的底物，在适合连接酶活性的条件下孵育由此接触的样品; 并且具体地确定由连接酶对底物核酸分子的作用产生的连接的核酸分子的存在和/或量，以指示表达微生物的连接酶的存在。 微生物可以是真菌或细菌或两者。 可以使用高pH条件来灭活哺乳动物连接酶。 描述相关工具包。

    Kits and Devices for Performing Methods of Detecting Viability-Associated Molecules
    5.
    发明申请
    Kits and Devices for Performing Methods of Detecting Viability-Associated Molecules 审中-公开
    用于检测活力相关分子的方法的试剂盒和装置

    公开(公告)号：US20110212438A1

    公开(公告)日：2011-09-01

    申请号：US13071108

    申请日：2011-03-24

    申请人： Stuart Wilson ,  Sharon Banin

    发明人： Stuart Wilson ,  Sharon Banin

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/25 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q2563/143 ,  C12Q2561/125 ,  C12Q2521/543

    摘要： Kits and devices for performing a method of detecting a molecule associated with viability of one or more cells or organisms in a sample are provided. The method comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase. The kits and devices for performing the method have numerous applications, in particular in monitoring viability of cells, toxicology testing and determining whether there is contamination in a sample or on a surface.

    摘要翻译： 提供了用于执行检测与样品中一种或多种细胞或生物体的活力相关的分子的方法的试剂盒和装置。 该方法包括使样品与酶接触的初始步骤，该酶能够在与一种或多种细胞或生物体的活力相关的分子存在下向核酸分子添加或除去化学部分。 由此产生新的可检测的核酸分子。 下一步涉及通过检测仅在与一种或多种细胞或生物体的活力相关的分子存在下产生的新的核酸分子来检测与一种或多种细胞或生物体的存活力相关的分子的存在。 与活力相关的最优选的分子是ATP，尽管也可以检测到NAD。 用于该方法的优选酶是连接酶。 用于执行该方法的试剂盒和装置具有许多应用，特别是在监测细胞的活力，毒理学测试和确定样品或表面是否存在污染。

    Assembly including vertical and horizontal joined circuit panels
    7.
    发明申请
    Assembly including vertical and horizontal joined circuit panels 有权
    组装包括垂直和水平连接电路板

    公开(公告)号：US20050269693A1

    公开(公告)日：2005-12-08

    申请号：US11143201

    申请日：2005-06-02

    申请人： Ronald Green ,  Sridhar Krishnan ,  Stuart Wilson ,  James Shook ,  Ming Tsai ,  Andy Stavros

    发明人： Ronald Green ,  Sridhar Krishnan ,  Stuart Wilson ,  James Shook ,  Ming Tsai ,  Andy Stavros

    IPC分类号： H01L23/34 ,  H05K1/14 ,  H05K3/00 ,  H05K3/34 ,  H05K3/36 ,  H05K3/40

    CPC分类号： H05K3/368 ,  H05K1/145 ,  H05K3/0052 ,  H05K3/3442 ,  H05K3/366 ,  H05K3/403 ,  H05K3/4092 ,  H05K2201/0397 ,  H05K2201/048 ,  H05K2201/0715 ,  H05K2201/09036 ,  H05K2201/09181 ,  H05K2201/09845 ,  H05K2201/0999 ,  H05K2201/10484

    摘要： An assembly is provided which includes a first circuit panel having a top surface, a first dielectric element and first conductive traces disposed on the first dielectric element. In addition, a second circuit panel has a bottom surface, a second dielectric element and second conductive traces disposed on the second dielectric element, where at least a portion of the second circuit panel overlies at least a portion of the first circuit panel. The assembly further includes an interconnect circuit panel having a third dielectric element which has a front surface, a rear surface opposite the front surface, a top end extending between the front and rear surfaces, a bottom end extending between the front and rear surfaces, and a plurality of interconnect traces disposed on the dielectric element. The bottom end of the interconnect element abuts the top surface of the first circuit panel and the top end abuts the bottom surface of the second circuit panel, where at least some of the first conductive traces are in conductive communication with the second conductive traces through the interconnect traces.

    摘要翻译： 提供了一种组件，其包括具有顶表面的第一电路板，设置在第一介电元件上的第一介电元件和第一导电迹线。 此外，第二电路板具有底表面，第二介电元件和设置在第二电介质元件上的第二导电迹线，其中第二电路板的至少一部分覆盖在第一电路板的至少一部分上。 该组件还包括具有第三电介质元件的互连电路板，第三电介质元件具有前表面，与前表面相对的后表面，在前表面和后表面之间延伸的顶端，在前表面和后表面之间延伸的底端，以及 布置在电介质元件上的多个互连迹线。 互连元件的底端邻接第一电路板的顶表面，并且顶端邻接第二电路板的底表面，其中至少一些第一导电迹线通过第二导电迹线与第二导电迹线导通连通 互连线。

    Modular building panels and method of constructing walls from the same
    8.
    发明授权
    Modular building panels and method of constructing walls from the same 失效
    模块化建筑面板和从其构建墙壁的方法

    公开(公告)号：US06427407B1

    公开(公告)日：2002-08-06

    申请号：US09466441

    申请日：1999-12-17

    申请人： Stuart Wilson

    发明人： Stuart Wilson

    IPC分类号： E04C234

    CPC分类号： E04C2/384 ,  E04B1/08 ,  E04B1/14

    摘要： A method for constructing prefabricated modular building panels includes providing a plurality of full wall height panels in several widths and full wall height corner elements which can be assembled to provide walls for a building of almost any size. The method also includes providing less than full height header and sill panels in several widths which can be used to install windows and doors. A window is installed between a header and sill panel having a width which is greater than the width of the window by placing studs between the header and sill panels at each side of the window and at each side of the header and sill panels. A door is installed under a header panel having a width which is greater than the width of the door by building a frame on each side of the door below the header panel. The panels are made with a frame of interconnected steel studs and steel top and bottom tracks. Pieces of structural building material are rigidly attached to the tracks and project from the front edges of the track by a predetermined distance. Strips of insulating material attached to the front edges of the studs have a thickness substantially equal to this first predetermined distance.

    摘要翻译： 一种用于构造预制模块化建筑面板的方法包括提供若干宽度的多个全壁高度面板和全壁高角部元件，其可组装成为几乎任何尺寸的建筑物提供墙壁。 该方法还包括提供少于全高度头部和底板的几种宽度，可用于安装窗户和门。 通过在窗口的每一侧和头部和门槛板的每一侧放置螺栓在头部和门槛板之间，将头部和门槛板之间的窗口安装在具有大于窗口宽度的宽度的窗口。 通过在门板下方的门的每一侧构建框架，将门安装在具有大于门宽度的宽度的头板下方。 这些面板由一个相互连接的钢螺柱和钢顶部和底部轨道的框架制成。 结构建筑材料的块刚性地附接到轨道并从轨道的前边缘突出预定距离。 附接到螺柱的前边缘的绝缘材料条具有基本上等于该第一预定距离的厚度。

    Purine derivatives
    9.
    发明授权
    Purine derivatives 有权
    嘌呤衍生物

    公开(公告)号：US08846696B2

    公开(公告)日：2014-09-30

    申请号：US12489663

    申请日：2009-06-23

    申请人： Peter Martin Fischer ,  Michael Jarman ,  Edward McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

    发明人： Peter Martin Fischer ,  Michael Jarman ,  Edward McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

    IPC分类号： A61K31/52 ,  C07D473/16

    CPC分类号： C07D473/16 ,  A61K31/52 ,  Y02A50/411

    摘要： The present invention relates to compounds of formula 1 or pharmaceutically acceptable salts thereof, wherein one of R1 and R2 is methyl, ethyl or isopropyl, and the other is H; R3 and R4 are each independently H, branched or unbranched C1-C6 alkyl, or aryl, and wherein at least one of R3 and R4 is other than H; R5 is a branched or unbranched C1-C5 alkyl group or a C1-C6 cycloalkyl group, each of which may be optionally substituted with one or more OH groups; R6, R7, R8 and R9 are each independently H, halogen, NO2, OH, OMe, CN, NH2, COOH, CONH2, or SO2NH2. A further aspect of the invention relates to pharmaceutical compositions comprising compounds of formula 1, and the use of said compounds in treating proliferative disorders, viral disorders, stroke, alopecia, CNS disorders, neurodegenerative disorders, or diabetes.

    摘要翻译： 本发明涉及式1化合物或其药学上可接受的盐，其中R 1和R 2之一是甲基，乙基或异丙基，另一个是H; R 3和R 4各自独立地为H，支链或非支链的C 1 -C 6烷基或芳基，并且其中R 3和R 4中的至少一个不是H; R5是支链或非支链C 1 -C 5烷基或C 1 -C 6环烷基，其各自可以任选被一个或多个OH基取代; R6，R7，R8和R9各自独立地为H，卤素，NO2，OH，OMe，CN，NH2，COOH，CONH2或SO2NH2。 本发明的另一方面涉及包含式1化合物的药物组合物，以及所述化合物在治疗增殖性疾病，病毒性疾病，中风，脱发，中枢神经系统疾病，神经变性疾病或糖尿病中的用途。

    Purine derivatives
    10.
    发明授权
    Purine derivatives 有权
    嘌呤衍生物

    公开(公告)号：US07544689B2

    公开(公告)日：2009-06-09

    申请号：US11238533

    申请日：2005-09-28

    申请人： Peter Martin Fischer ,  Michael Jarman ,  Edward McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

    发明人： Peter Martin Fischer ,  Michael Jarman ,  Edward McDonald ,  Bernard Nutley ,  Florence Raynaud ,  Stuart Wilson ,  Paul Workman

    IPC分类号： C07D473/16 ,  A61K31/52 ,  A61P35/00 ,  A61P3/10 ,  A61P31/20 ,  A61P31/14 ,  A61P17/06 ,  A61P35/02 ,  A61P25/28

    CPC分类号： C07D473/16

    摘要： The present invention relates to compounds of formula I or pharmaceutically acceptable salts thereof, wherein one of R1 and R2 is methyl, ethyl or isopropyl, and the other is H; R3 and R4 are each independently H, branched or un branched C1-C6 alkyl, or aryl, and wherein at least one of R3 and R4 is other than H; R5 is a branched or unbranched C1-C5 alkyl group or a C1-C6 cycloalkyl group, each of which may be optionally substituted with one or more OH groups; R6, R7, R8 and R9 are each independently H, halogen, NO2, OH, OMe, CN, NH2, COOH, CONH2, or SO2NH2. A further aspect of the invention relates to pharmaceutical compositions comprising compounds of formula 1, and the use of said compounds in treating proliferative disorders, viral disorders, CNS disorders, diabetes, stroke, alopecia or neurodegenerative disorders.

    摘要翻译： 本发明涉及式I化合物或其药学上可接受的盐，其中R 1和R 2之一是甲基，乙基或异丙基，另一个是H; R 3和R 4各自独立地为H，支链或非支链C 1 -C 6烷基或芳基，并且其中R 3和R 4中的至少一个不是H; R5是支链或非支链C 1 -C 5烷基或C 1 -C 6环烷基，其各自可以任选被一个或多个OH基取代; R6，R7，R8和R9各自独立地为H，卤素，NO2，OH，OMe，CN，NH2，COOH，CONH2或SO2NH2。 本发明的另一方面涉及包含式1化合物的药物组合物，以及所述化合物在治疗增殖性疾病，病毒性疾病，中枢神经系统疾病，糖尿病，中风，脱发或神经变性疾病中的用途。