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    Probe density self-considerations and elongation of complementary looped probes where probes are attached to a solid phase
    1.
    发明授权
    Probe density self-considerations and elongation of complementary looped probes where probes are attached to a solid phase 有权
    探针密度自我考虑和互补环状探针的伸长，其中探针连接到固相

    公开(公告)号：US08795966B2

    公开(公告)日：2014-08-05

    申请号：US12708362

    申请日：2010-02-18

    Applicant: Michael Seul ,  Yi Zhang ,  Sukanta Banerjee ,  Jiacheng Yang ,  Chiu Chau

    Inventor： Michael Seul ,  Yi Zhang ,  Sukanta Banerjee ,  Jiacheng Yang ,  Chiu Chau

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/6837 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q2565/1015 ,  C12Q2537/143 ,  C12Q2533/101 ,  C12Q2535/125 ,  C12Q2525/301

    Abstract: In a multiplexed assay method carried out in solution, wherein the solution contains nucleic acid targets and, wherein several different types of oligonucleotide probes, each type having a different sequence in a region designated as a target binding domain, are used to detect the nucleic acid targets, said assay method including a method for increasing the effective concentration of the nucleic acid targets at the surface of a bead to which the oligonucleotide probes are bound, by one or more of the following steps:adjusting assay conditions so as to increase the effective concentration of the targets available for binding to the probes, by one or more of the following: (i) selecting a particular probe density on the surface of the bead; (ii) selecting a solution having an ionic strength greater than a threshold; (ii) selecting a target domain of a size less than a threshold; or (iii) selecting target domains within a specified proximity to a terminal end of the targets.

    Abstract translation: 在溶液中进行的多重测定方法中，其中溶液含有核酸靶，并且其中使用几个不同类型的寡核苷酸探针，每个类型在被称为靶结合结构域的区域中具有不同序列，以检测核酸 目标，所述测定方法包括通过一个或多个以下步骤增加寡核苷酸探针结合珠粒表面上的核酸靶的有效浓度的方法：调节测定条件以增加有效 通过一种或多种以下方法可用于结合探针的靶的浓度：（i）在珠的表面上选择特定的探针密度; （ii）选择具有大于阈值的离子强度的溶液; （ii）选择小于阈值的大小的目标域; 或（iii）在目标的终端的指定接近范围内选择目标域。

    Method For Selecting Desired Level Of Dye Loading And Controlling Loading Of Polymer Microparticles
    3.
    发明申请
    Method For Selecting Desired Level Of Dye Loading And Controlling Loading Of Polymer Microparticles 审中-公开
    选择染料加载和控制聚合物微粒负载水平的方法

    公开(公告)号：US20120220715A1

    公开(公告)日：2012-08-30

    申请号：US13417791

    申请日：2012-03-12

    Applicant: Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

    Inventor： Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

    IPC: C08L25/08

    CPC classification number: C08J3/212 ,  C08J3/215 ,  C08J3/22 ,  C08J2325/08

    Abstract: Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.

    Abstract translation: 通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。 三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。 第二溶剂是用于溶质和聚合物（调谐溶剂）的弱溶剂或非溶剂。 第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂，但是作为相对于第一和第二溶剂的共溶剂，因为其可与第一溶剂和第二溶剂混溶。 通过调节溶质相对于微粒聚合物的比例，调节三元溶剂体系的组成，主要是调谐溶剂的量来控制掺入微粒中的溶质量。 该方法对于提供包含可区分的染料负载，特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。

    Method for controlling solute loading of polymer microparticles
    5.
    发明授权
    Method for controlling solute loading of polymer microparticles 有权
    控制聚合物微粒溶质负载的方法

    公开(公告)号：US07255895B2

    公开(公告)日：2007-08-14

    申请号：US10348165

    申请日：2003-01-21

    Applicant: Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

    Inventor： Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

    IPC: B05D7/00

    CPC classification number: C08J3/212 ,  C08J3/215 ,  C08J3/22 ,  C08J2325/08

    Abstract: Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.

    Abstract translation: 通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。 三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。 第二溶剂是用于溶质和聚合物（调谐溶剂）的弱溶剂或非溶剂。 第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂，但是作为相对于第一和第二溶剂的共溶剂，因为其可与第一溶剂和第二溶剂混溶。 通过调节溶质相对于微粒聚合物的比例，调节三元溶剂体系的组成，主要是调谐溶剂的量来控制掺入微粒的溶质量。 该方法对于提供包含可区分的染料负载，特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。

    Un-supported polymeric film with embedded microbeads
    7.
    发明授权
    Un-supported polymeric film with embedded microbeads 有权
    具有嵌入微珠的未负载的聚合物膜

    公开(公告)号：US09436088B2

    公开(公告)日：2016-09-06

    申请号：US11761789

    申请日：2007-06-12

    Applicant: Michael Seul ,  Sukanta Banerjee ,  Kairali Podual ,  Ye Hong

    Inventor： Michael Seul ,  Sukanta Banerjee ,  Kairali Podual ,  Ye Hong

    IPC: G01N33/553 ,  G03F7/16 ,  G03F7/004

    CPC classification number: C08J3/075 ,  C08J3/28 ,  C08J2333/26 ,  C08J2425/06 ,  C12Q1/6837 ,  G01N33/5005 ,  G01N33/545 ,  G03F7/0047 ,  G03F7/164 ,  Y10T428/249952 ,  Y10T428/25

    Abstract: The present invention relates to polymer-bead composites having a single layer planar, crystalline assembly of encoded beads embedded in a hydrophilic polymeric matrix. The composite may be unattached to a solid support. The encoded beads have different biomolecules attached to their surfaces, and the encoding permits distinguishing beads having different biomolecules attached thereto. The present invention also relates to a systematic process for the creation of functionally organized, spatially patterned assemblies of polymer-microparticle composites, including the AC electric field-mediated assembly of patterned, self-supporting organic (polymeric) films and organic-polymer-microparticle composites of tailored composition and morphology. The present invention also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, and micro-reactors, and miniaturized format for generation of multifunctional thin films.

    Abstract translation: 本发明涉及具有嵌入亲水性聚合物基质中的编码珠粒的单层平面结晶组合物的聚合物 - 珠复合物。 复合材料可能不附着在固体支撑物上。 编码的珠粒具有连接到其表面的不同生物分子，并且编码允许区分附着有不同生物分子的珠粒。 本发明还涉及一种用于创建聚合物 - 微粒复合材料的功能组织的空间图案化组件的系统方法，包括图案化的自支撑有机（聚合物）膜和有机 - 聚合物 - 微粒的AC电场介导装配 定制组合和形态的复合材料。 本发明还涉及这种功能组件在材料科学和生物学中的应用。 其他应用领域包括传感器，催化剂，膜和微反应器以及用于产生多功能薄膜的小型化形式。

    PROBE DENSITY SELF-CONSIDERATIONS AND ELONGATION OF COMPLEMENTARY LOOPED PROBES WHERE PROBES ARE ATTACHED TO A SOLID PHASE
    8.
    发明申请
    PROBE DENSITY SELF-CONSIDERATIONS AND ELONGATION OF COMPLEMENTARY LOOPED PROBES WHERE PROBES ARE ATTACHED TO A SOLID PHASE 有权
    探索密度自我考虑和延伸的复杂循环探测器，其中探测器连接到固相

    公开(公告)号：US20100267578A1

    公开(公告)日：2010-10-21

    申请号：US12708362

    申请日：2010-02-18

    Applicant: MICHAEL SEUL ,  Yi Zhang ,  Sukanta Banerjee ,  Jiacheng Yang ,  Chiu Chau

    Inventor： MICHAEL SEUL ,  Yi Zhang ,  Sukanta Banerjee ,  Jiacheng Yang ,  Chiu Chau

    IPC: C40B30/04

    CPC classification number: C12Q1/6837 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q2565/1015 ,  C12Q2537/143 ,  C12Q2533/101 ,  C12Q2535/125 ,  C12Q2525/301

    Abstract: In a multiplexed assay method carried out in solution, wherein the solution contains nucleic acid targets and, wherein several different types of oligonucleotide probes, each type having a different sequence in a region designated as a target binding domain, are used to detect the nucleic acid targets, said assay method including a method for increasing the effective concentration of the nucleic acid targets at the surface of a bead to which the oligonucleotide probes are bound, by one or more of the following steps:adjusting assay conditions so as to increase the effective concentration of the targets available for binding to the probes, by one or more of the following: (i) selecting a particular probe density on the surface of the bead; (ii) selecting a solution having an ionic strength greater than a threshold; (ii) selecting a target domain of a size less than a threshold; or (iii) selecting target domains within a specified proximity to a terminal end of the targets.

    Abstract translation: 在溶液中进行的多重测定方法中，其中溶液含有核酸靶，并且其中使用几个不同类型的寡核苷酸探针，每个类型在被称为靶结合结构域的区域中具有不同序列，以检测核酸 目标，所述测定方法包括通过一个或多个以下步骤增加寡核苷酸探针结合珠粒表面上的核酸靶的有效浓度的方法：调节测定条件以增加有效 通过一种或多种以下方法可用于结合探针的靶的浓度：（i）在珠的表面上选择特定的探针密度; （ii）选择具有大于阈值的离子强度的溶液; （ii）选择小于阈值的大小的目标域; 或（iii）在目标的终端的指定接近范围内选择目标域。

    Method for controlling solute loading of polymer microparticles
    9.
    发明授权
    Method for controlling solute loading of polymer microparticles 有权
    控制聚合物微粒溶质负载的方法

    公开(公告)号：US07498054B2

    公开(公告)日：2009-03-03

    申请号：US11179135

    申请日：2005-07-12

    Applicant: Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

    Inventor： Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

    IPC: B05D7/00 ,  C09D11/00

    CPC classification number: C08J3/212 ,  C08J3/215 ,  C08J3/22 ,  C08J2325/08

    Abstract: Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.

    Abstract translation: 通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。 三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。 第二溶剂是用于溶质和聚合物（调谐溶剂）的弱溶剂或非溶剂。 第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂，但是作为相对于第一和第二溶剂的共溶剂，因为其可与第一溶剂和第二溶剂混溶。 通过调节溶质相对于微粒聚合物的比例，调节三元溶剂体系的组成，主要是调谐溶剂的量来控制掺入微粒的溶质量。 该方法对于提供包含可区分的染料负载，特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。

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