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    Modified E. coli enterotoxin II signal peptide and a microorganism expressing a fusion protein of a said peptide and a heterologous protein
    4.
    发明授权
    Modified E. coli enterotoxin II signal peptide and a microorganism expressing a fusion protein of a said peptide and a heterologous protein 有权
    修饰的大肠杆菌肠毒素II信号肽和表达所述肽和异源蛋白质的融合蛋白的微生物

    公开(公告)号：US06605697B1

    公开(公告)日：2003-08-12

    申请号：US09786569

    申请日：2001-03-07

    申请人： Se-Chang Kwon ,  Sung-Youb Jung ,  Hoon Shin ,  Jay-Do Choi ,  Ki-Doo Choi ,  Gwan-Sun Lee

    发明人： Se-Chang Kwon ,  Sung-Youb Jung ,  Hoon Shin ,  Jay-Do Choi ,  Ki-Doo Choi ,  Gwan-Sun Lee

    IPC分类号： C07K100

    CPC分类号： C12N15/70 ,  C07K14/245 ,  C07K14/61 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/75 ,  C12N15/625

    摘要： A heterologous protein is produced by: (i) culturing a microorganism transformed with an expression vector comprising a gene encoding a modified E. coli enterotoxin II signal peptide fused with the heterologous protein to produce and secrete the heterologous protein to periplasm, the modified E. coli enterotoxin II signal peptide being obtained by replacing at least one of the 2nd, 4th, 5th, 12th, 20th, and 22nd amino acids of E. coli enterotoxin II signal peptide of the following amino acid sequence (SEQ ID NO: 1) with another amino acid, with the proviso that at least one of the 2nd and 4th amino acid of the modified peptide is lysine; and (ii) recovering the heterologous protein from the periplasm.

    摘要翻译： 通过以下方式产生异源蛋白质：（i）培养用包含编码与异源蛋白质融合的修饰的大肠杆菌肠毒素II信号肽的基因的表达载体转化的微生物，以产生和分泌异源蛋白质至周质，修饰的E. 通过将下述氨基酸序列（SEQ ID NO：1）的大肠杆菌肠毒素II信号肽的第2，第4，第5，第12，第20和第22氨基酸中的至少一个替换为大肠杆菌肠毒素II信号肽， 另一氨基酸，条件是修饰肽的第二和第四氨基酸中的至少一个是赖氨酸; 和（ii）从周质中回收异源蛋白质。