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1.发明申请公开(公告)号：US20110033906A1
公开(公告)日：2011-02-10
申请号：US12850966
申请日：2010-08-05
申请人： Sung-Jin JO , Risa Nakamura , Satoshi Katahira , Nobuhiro Ishida , Haruo Takahashi , Kazuhide Tabata , Kazunori Nakashima , Chiaki Ogino , Akihiko Kondo , Naoko Takahashi
发明人： Sung-Jin JO , Risa Nakamura , Satoshi Katahira , Nobuhiro Ishida , Haruo Takahashi , Kazuhide Tabata , Kazunori Nakashima , Chiaki Ogino , Akihiko Kondo , Naoko Takahashi
IPC分类号： C12P7/06
CPC分类号： C12P7/10 , C12P2201/00 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a method for producing a useful substance efficiently from cellulose without using any cellulase preparation. According to the disclosures of the present specification, in the production of a useful substance from a cellulose-containing material, the cellulose-containing material is brought into contact with an ionic liquid to cause the ionic liquid to permeate the cellulose-containing material, and a carbon source comprising the cellulose in the cellulose-containing material is simultaneously saccharified and fermented by a cellulase-producing microorganism in the presence of the ionic liquid.
摘要翻译： 本发明提供一种在不使用任何纤维素酶制剂的情况下有效地从纤维素制备有用物质的方法。 根据本说明书的公开内容，在从含纤维素材料制造有用物质时，使含纤维素材料与离子液体接触，使离子液体渗透含纤维素材料， 含有纤维素的材料中的纤维素的碳源同时在离子液体存在下被纤维素酶微生物糖化并发酵。
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2.发明授权公开(公告)号：US09587254B2
公开(公告)日：2017-03-07
申请号：US12850966
申请日：2010-08-05
申请人： Sung-Jin Jo , Risa Nakamura , Satoshi Katahira , Nobuhiro Ishida , Haruo Takahashi , Naoko Takahashi , Kazuhide Tabata , Kazunori Nakashima , Chiaki Ogino , Akihiko Kondo
发明人： Sung-Jin Jo , Risa Nakamura , Satoshi Katahira , Nobuhiro Ishida , Haruo Takahashi , Kazuhide Tabata , Kazunori Nakashima , Chiaki Ogino , Akihiko Kondo
IPC分类号： C12P7/10
CPC分类号： C12P7/10 , C12P2201/00 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a method for producing a useful substance efficiently from cellulose without using any cellulase preparation. According to the disclosures of the present specification, in the production of a useful substance from a cellulose-containing material, the cellulose-containing material is brought into contact with an ionic liquid to cause the ionic liquid to permeate the cellulose-containing material, and a carbon source comprising the cellulose in the cellulose-containing material is simultaneously saccharified and fermented by a cellulase-producing microorganism in the presence of the ionic liquid.
摘要翻译： 本发明提供一种在不使用任何纤维素酶制剂的情况下有效地从纤维素制备有用物质的方法。 根据本说明书的公开内容，在从含纤维素材料制造有用物质时，使含纤维素材料与离子液体接触，使离子液体渗透含纤维素材料， 含有纤维素的材料中的纤维素的碳源同时在离子液体存在下被纤维素酶微生物糖化并发酵。
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3.发明申请METHOD FOR TREATMENT OF CELLULOSIC BIOMASS AND METHOD FOR PRODUCTION OF SUGAR, ALCOHOL, OR ORGANIC ACID FROM CELLULOSIC BIOMASS 审中-公开用于处理细胞生物量的方法和从细胞生物量生产糖，醇或有机酸的方法公开(公告)号：US20130302854A1
公开(公告)日：2013-11-14
申请号：US13981975
申请日：2012-01-19
IPC分类号： C08B1/00
CPC分类号： C08B1/003 , A23V2002/00 , C08H8/00 , C12P7/02 , C12P7/10 , C12P7/40 , C12P19/02 , C12P2201/00 , C12P2203/00 , C13K1/02 , Y02E50/16 , Y02E50/17 , A23V2250/5108 , A23V2250/51088
摘要： This invention is intended to improve saccharification efficiency by allowing cellulose and/or hemicellulose to relax sufficiently with an ionic liquid. Cellulose and/or hemicellulose is allowed to relax sufficiently with an ionic liquid through a step of soaking cellulosic biomass in a solution containing an ionic liquid and an alkali.
摘要翻译： 本发明旨在通过使离子液体使纤维素和/或半纤维素充分松弛来提高糖化效率。 使纤维素和/或半纤维素通过将纤维素生物质浸泡在含有离子液体和碱的溶液中的步骤，通过离子液体充分松弛。
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公开(公告)号：US20130130328A1
公开(公告)日：2013-05-23
申请号：US13702166
申请日：2011-06-07
IPC分类号： C08B37/00
CPC分类号： C08B37/0003 , C12P7/06 , C12P19/02 , Y02E50/17 , Y02E50/343
摘要： Plant biomass is immersed in a solution that contains a polar solvent and an imidazolium salt that has a melting point of at least 100° C. As a result, the cellulose and hemicellulose present in the plant biomass are relaxed (decrystallized and depolymerized) and brought into an easy-to-degrade state. Reacting the immersed plant biomass with a cellulase produces saccharide at a high conversion efficiency.
摘要翻译： 将植物生物质浸入含有极性溶剂和熔点至少为100℃的咪唑盐的溶液中。结果，植物生物质中存在的纤维素和半纤维素被松弛（去结晶和解聚）并带来 成为易于降解的状态。 用纤维素酶反应浸入的植物生物质以高转化效率产生糖。
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5.发明申请DNA Encoding a Protein Having a D-Lactate Dehydrogenase Activity and Uses Thereof 有权编码具有D-乳酸脱氢酶活性的蛋白质的DNA及其用途公开(公告)号：US20090275095A1
公开(公告)日：2009-11-05
申请号：US12324804
申请日：2008-11-26
申请人： Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
发明人： Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
CPC分类号： C12N9/0006 , C12P7/56 , C12P7/625
摘要： This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).
摘要翻译： 本发明提供编码具有乳酸脱氢酶活性的蛋白质的多核苷酸和可用于生产D-乳酸的蛋白质。 该多核苷酸具有SEQ ID NO：1（a）所示的核苷酸序列，并且在严格条件下与包含SEQ ID NO：1所示的全部或部分核苷酸序列或其互补链的探针杂交，并编码 具有D-乳酸脱氢酶活性的蛋白质（b）。
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公开(公告)号：US07696333B1
公开(公告)日：2010-04-13
申请号：US10578614
申请日：2004-11-05
申请人： Nobuhiro Ishida , Kenro Tokuhiro , Eiji Nagamori , Haruo Takahashi , Satoshi Saitoh , Tohru Ohnishi
发明人： Nobuhiro Ishida , Kenro Tokuhiro , Eiji Nagamori , Haruo Takahashi , Satoshi Saitoh , Tohru Ohnishi
CPC分类号： C12N15/81
摘要： The invention provides a promoter that can be used in the presence of an organic acid. The promoter includes DNA with promoter activity of high osmolarity response 7 gene (HOR7 gene), glycelaldehyde 3 phosphate dehydrogenase 2 gene (TDH2 gene), heat shock protein 30 gene (HSP30), hexose transport protein 7 gene (HXT7 gene), thioredoxin peroxidase 1 gene (AHP1 gene), or membrane protein 1 associated gene gene) of yeasts.
摘要翻译： 本发明提供可在有机酸存在下使用的促进剂。 启动子包括具有高渗透压反应7基因（HOR7基因），甘油醛3磷酸脱氢酶2基因（TDH2基因），热休克蛋白30基因（HSP30），己糖转运蛋白7基因（HXT7基因），硫氧还蛋白过氧化物酶 1基因（AHP1基因）或膜蛋白1相关基因基因）。
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7.发明授权DNA encoding a protein having D-lactate dehydrogenase activity and uses thereof 有权编码具有D-乳酸脱氢酶活性的蛋白质的DNA及其用途公开(公告)号：US07964382B2
公开(公告)日：2011-06-21
申请号：US12324804
申请日：2008-11-26
申请人： Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
发明人： Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
IPC分类号： C12N9/04 , C12N1/20 , C12N15/00 , C12P7/56 , C12P21/04 , C12Q1/00 , C12Q1/68 , C12Q1/32 , C07H21/04
CPC分类号： C12N9/0006 , C12P7/56 , C12P7/625
摘要： This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).
摘要翻译： 本发明提供编码具有乳酸脱氢酶活性的蛋白质的多核苷酸和可用于生产D-乳酸的蛋白质。 该多核苷酸具有SEQ ID NO：1（a）所示的核苷酸序列，并且在严格条件下与包含SEQ ID NO：1所示的全部或部分核苷酸序列或其互补链的探针杂交，并编码 具有D-乳酸脱氢酶活性的蛋白质（b）。
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8.发明申请Dna encoding a protein having d-lactate dehydrogenase activity and uses thereof 审中-公开Dna编码具有d-乳酸脱氢酶活性的蛋白质及其用途公开(公告)号：US20070105202A1
公开(公告)日：2007-05-10
申请号：US10557306
申请日：2004-05-21
申请人： Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
发明人： Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
CPC分类号： C12N9/0006 , C12P7/56 , C12P7/625
摘要： This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).
摘要翻译： 本发明提供编码具有乳酸脱氢酶活性的蛋白质的多核苷酸和可用于生产D-乳酸的蛋白质。 该多核苷酸具有SEQ ID NO：1（a）所示的核苷酸序列，并且在严格条件下与包含SEQ ID NO：1所示的全部或部分核苷酸序列或其互补链的探针杂交，并编码 具有D-乳酸脱氢酶活性的蛋白质（b）。
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公开(公告)号：US20070072267A1
公开(公告)日：2007-03-29
申请号：US11529443
申请日：2006-09-29
CPC分类号： C12N15/81 , C07K14/395 , C12N15/815
摘要： The present invention provides a transformant having a gene expression inducing system having better responsiveness. The transformant of the invention has a galactose induction system and retains a coding region having a galactose induction system and encoding an assisting protein assisting transcription activation by a protein having the Gal4 activity under control of a constitutive promoter, and a coding region encoding an endogenous or exogenous desired protein under control of a galactose-inducible promoter.
摘要翻译： 本发明提供具有更好反应性的基因表达诱导体系的转化体。 本发明的转化体具有半乳糖诱导系统，并保留了具有半乳糖诱导系统的编码区，并且编码辅助蛋白质，其辅助转录激活由具有组成型启动子控制下的Gal4活性的蛋白质和编码区域编码内源或 在半乳糖诱导型启动子控制下的外源性所需蛋白质。
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公开(公告)号：US20090104675A1
公开(公告)日：2009-04-23
申请号：US12275099
申请日：2008-11-20
申请人： Ikuo Yamaguchi , Osamu Saotome , Shigeru Kuromiya , Tohru Ohnishi , Noriko Yasutani , Satoshi Saitoh , Makoto Mouri , Mitsuru Nakano , Arimitsu Usuki , Nobuhiro Ishida , Kenro Tokuhiro , Eiji Nagamori , Haruo Takahashi
发明人： Ikuo Yamaguchi , Osamu Saotome , Shigeru Kuromiya , Tohru Ohnishi , Noriko Yasutani , Satoshi Saitoh , Makoto Mouri , Mitsuru Nakano , Arimitsu Usuki , Nobuhiro Ishida , Kenro Tokuhiro , Eiji Nagamori , Haruo Takahashi
IPC分类号： C12P7/56
CPC分类号： C12P7/56 , C12N9/0006
摘要： Lactic acid with high optical purity that has not previously been achieved is produced.It has been found that the optical purity of lactic acid is reduced as the racemization reaction of lactic acid proceeds when lactic acid coexists with glycerol. By reducing the amount of glycerol prior to concentrating lactic acid by heating, the optical purity of lactic acid after concentration by heating can be maintained at a high level.
摘要翻译： 产生以前未实现的具有高光学纯度的乳酸。 已经发现，当乳酸与甘油共存时，随着乳酸的外消旋反应的进行，乳酸的光学纯度降低。 在通过加热浓缩乳酸之前减少甘油的量，通过加热浓缩后的乳酸的光学纯度可以保持在高水平。
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