公开(公告)号：US20030170914A1

公开(公告)日：2003-09-11

申请号：US10357679

申请日：2003-02-03

Applicant: SurModics, Inc

Inventor： Patrick E. Guire ,  Melvin J. Swanson

IPC: C12Q001/68 ,  G01N033/543

CPC classification number: C12Q1/6837 ,  B01J19/0046 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S977/849

Abstract: A system for producing substantially identical specific binding ligand (e.g., nucleic acid) probe arrays, for instance, by preparing and replicating an original master array and/or by providing a reusable assay array that is capable of being regenerated. In one embodiment the system includes the preparation and use of a) a master array surface having address sequences immobilized in the form of a patterned, and optionally random, array, b) a multi-ligand conjugate having a binding domain complementary to an address sequence, a binding domain complementary to a target sequence, and a third ligand for use in forming (e.g., by binding or polymerization) the conjugates into or onto the surface of assay array, which can be used with or upon disassociation of the address and its complementary sequences.

Abstract translation: 用于制备基本相同的特异性结合配体（例如，核酸）探针阵列的系统，例如通过制备和复制原始主阵列和/或通过提供能够再生的可重复使用的分析阵列。 在一个实施方案中，该系统包括a）主阵列表面的制备和使用，该主阵列表面具有以图案化和任选的阵列的形式固定的地址序列，b）具有与地址序列互补的结合结构域的多配体缀合物 ，与靶序列互补的结合结构域，以及用于将缀合物形成（例如通过结合或聚合）到测定阵列表面中或表面上的第三配体，其可以在解除地址及其使用时使用 互补序列。

公开(公告)号：US20040209305A1

公开(公告)日：2004-10-21

申请号：US10844667

申请日：2004-05-12

Applicant: SurModics, Inc.

Inventor： Dale G. Swan ,  Melvin J. Swanson

IPC: C12Q001/68 ,  C08G063/48 ,  B05D003/00

CPC classification number: C40B80/00 ,  B01J2219/00317 ,  B01J2219/00531 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00711 ,  B01J2219/00716 ,  B01J2219/00722 ,  B82Y30/00 ,  C07B2200/11 ,  C07C45/63 ,  C07C235/84 ,  C07C323/42 ,  C07H21/00 ,  C08L63/00 ,  C09D4/00 ,  C12Q1/6834 ,  C40B40/06 ,  C40B60/14 ,  C07C49/813

Abstract: Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes epoxide groups capable of covalently binding to the target molecule. Optionally, the composition can contain photoreactive groups for use in attaching the reagent composition to the surface. The reagent composition can be used to provide activated slides for use in preparing microarrays of nucleic acids.

Abstract translation: 用于将目标分子（例如核酸）共价连接到基底表面上的方法和试剂组合物。 试剂组合物包括能够与靶分子共价结合的环氧基。 任选地，组合物可以含有用于将试剂组合物附着到表面上的光反应性基团。 试剂组合物可用于提供用于制备核酸微阵列的活性载玻片。

公开(公告)号：US20030148360A1

公开(公告)日：2003-08-07

申请号：US10346691

申请日：2003-01-15

Applicant: SurModics, Inc.

Inventor： Patrick E. Guire ,  Melvin J. Swanson

IPC: C12Q001/68 ,  G01N033/53 ,  C12M001/34 ,  G01N033/543

CPC classification number: C12Q1/6837 ,  B01J19/0046 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06 ,  C12Q2525/179 ,  C12Q2525/161 ,  C12Q2565/515

Abstract: A system for producing substantially identical specific binding ligand probe arrays, for instance, by preparing and replicating an original master array and/or by providing a reusable assay array that is capable of being regenerated. In one embodiment the system includes the preparation and use of a) a master array surface having address ligands immobilized thereon, b) a multi-ligand conjugate having a binding domain complementary to an address ligand, a binding domain complementary to a target ligand, and a third ligand for use in transferring the conjugates into or onto the surface of assay array, which can be used with or upon disassociation of the address and its complementary ligands.

Abstract translation: 用于制备基本相同的特异性结合配体探针阵列的系统，例如通过制备和复制原始主阵列和/或通过提供能够再生的可重复使用的测定阵列。 在一个实施方案中，该系统包括a）具有固定在其上的地址配体的主阵列表面的制备和用途，b）具有与地址配体互补的结合结构域，与靶配体互补的结合结构域的多配体缀合物，以及 用于将缀合物转移到测定阵列表面中或其上的第三配体，其可以与解离地址及其互补配体一起使用或分离。

公开(公告)号：US20030148308A1

公开(公告)日：2003-08-07

申请号：US10192917

申请日：2002-07-09

Applicant: SurModics, Inc.

Inventor： Ralph A. Chappa ,  Sheau-Ping Hu ,  Dale G. Swan ,  Melvin J. Swanson ,  Patrick E. Guire

IPC: C12Q001/68 ,  C12M001/34 ,  B05D003/00

CPC classification number: B82Y30/00 ,  B01J2219/00317 ,  B01J2219/00531 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00711 ,  B01J2219/00716 ,  B01J2219/00722 ,  C07B2200/11 ,  C07C45/63 ,  C07C235/84 ,  C07C323/42 ,  C07H21/00 ,  C12Q1/6834 ,  C40B40/06 ,  C40B60/14 ,  C07C49/813

Abstract: Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes groups capable of covalently binding to the target molecule. Optionally, the composition can contain photoreactive groups for use in attaching the reagent composition to the surface. The reagent composition can be used to provide activated slides for use in preparing microarrays of nucleic acids.