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公开(公告)号:US5702934A
公开(公告)日:1997-12-30
申请号:US238130
申请日:1994-05-04
申请人: Sven Hastrup , Sven Branner , Birthe Ravn J.o slashed.rgensen , Tove Christensen , Birgitte Bojer J.o slashed.rgensen , Jeffrey R. Shuster , Mark Madden , Donna L. Moyer , Claus Fuglsang
发明人: Sven Hastrup , Sven Branner , Birthe Ravn J.o slashed.rgensen , Tove Christensen , Birgitte Bojer J.o slashed.rgensen , Jeffrey R. Shuster , Mark Madden , Donna L. Moyer , Claus Fuglsang
IPC分类号: A61K39/12 , A61K39/395 , A61P31/04 , C07K14/025 , C12N20060101 , C12N9/00 , C12N9/54 , C12N9/58 , C12N15/09 , C12N15/37 , C12N15/62 , C12N15/80 , C12N15/86 , C12N15/863 , C12P21/06 , C12R1/69 , C12R1/77 , C12N1/15 , C12N9/76
CPC分类号: C12N9/58 , C07K14/005 , C12N15/80 , C12N15/86 , C12N7/00 , C12N9/54 , C12P21/06 , C07K2319/00 , C07K2319/40 , C12N2710/20022 , C12N2710/24143
摘要: The present invention is related to a process for producing an active enzyme comprising fermenting the proform of the active enzyme in the present of a proteolytic enzyme different from the active enzyme and capable of converting the proenzyme into an active enzyme as well as to host cells, recombinant expression vectors and host cells suitable for use in the process.
摘要翻译: 本发明涉及一种生产活性酶的方法,其包括在不同于活性酶的蛋白水解酶存在下发酵活性酶的形式并能够将酶转化为活性酶以及宿主细胞, 重组表达载体和适用于该过程的宿主细胞。
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公开(公告)号:US5807729A
公开(公告)日:1998-09-15
申请号:US442859
申请日:1995-05-17
IPC分类号: C12N15/09 , C12N1/19 , C12N9/58 , C12N9/62 , C12N15/00 , C12N15/57 , C12Q1/34 , C12Q1/37 , C12R1/69 , C12R1/77 , C12N15/80
CPC分类号: C12N9/58 , C12N9/62 , Y10S435/975
摘要: The present invention relates to a novel metalloprotease obtainable from a fungus having increased proteolytic activity. Additionally, the invention related to isolated nucleic acid fragments encoding said metalloprotease as well as vectors, DNA constructs, and recombinant host cells comprising said nucleic acid fragments.
摘要翻译: 本发明涉及从具有增加的蛋白水解活性的真菌获得的新的金属蛋白酶。 此外,本发明涉及编码所述金属蛋白酶的分离的核酸片段以及载体,DNA构建体和包含所述核酸片段的重组宿主细胞。
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公开(公告)号:US5843753A
公开(公告)日:1998-12-01
申请号:US398489
申请日:1995-03-03
IPC分类号: C12N15/09 , C12N1/19 , C12N9/58 , C12N9/62 , C12N15/00 , C12N15/57 , C12Q1/34 , C12Q1/37 , C12R1/69 , C12R1/77
CPC分类号: C12N9/58 , C12N9/62 , Y10S435/975
摘要: The present invention relates to a novel metalloprotease obtainable from a fungus having increased proteolytic activity. Additionally, the invention related to isolated nucleic acid fragments encoding said metalloprotease as well as vectors, DNA constructs, and recombinant host cells comprising said nucleic acid fragments.
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公开(公告)号:US5691162A
公开(公告)日:1997-11-25
申请号:US443104
申请日:1995-05-17
IPC分类号: C12N15/09 , C12N1/19 , C12N9/58 , C12N9/62 , C12N15/00 , C12N15/57 , C12Q1/34 , C12Q1/37 , C12R1/69 , C12R1/77
CPC分类号: C12N9/58 , C12N9/62 , Y10S435/975
摘要: The present invention relates to a novel metalloprotease obtainable from a fungus having increased proteolytic activity. Additionally, the invention related to isolated nucleic acid fragments encoding said metalloprotease as well as vectors, DNA constructs, and recombinant host cells comprising said nucleic acid fragments.
摘要翻译: 本发明涉及从具有增加的蛋白水解活性的真菌获得的新的金属蛋白酶。 此外,本发明涉及编码所述金属蛋白酶的分离的核酸片段以及载体,DNA构建体和包含所述核酸片段的重组宿主细胞。
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公开(公告)号:US06835821B2
公开(公告)日:2004-12-28
申请号:US10310730
申请日:2002-12-05
申请人: Sven Hastrup , Sven Branner , Fanny Norris , Steffen Bjorn Petersen , Leif Nørskov-Lauridsen , Villy Johannes Jensen , Dorrit Aaslyng
发明人: Sven Hastrup , Sven Branner , Fanny Norris , Steffen Bjorn Petersen , Leif Nørskov-Lauridsen , Villy Johannes Jensen , Dorrit Aaslyng
IPC分类号: C12N1557
摘要: The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.
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公开(公告)号:US5631217A
公开(公告)日:1997-05-20
申请号:US527734
申请日:1995-09-13
申请人: Sven Branner , Sven Hastrup , Nina Eriksen , Poul Lindegaard , Ole H. Olsen , Eric Casteleijn , Maarten R. Egmond , Johan Haverkamp , Wouter Musters , Jakob de Vlieg
发明人: Sven Branner , Sven Hastrup , Nina Eriksen , Poul Lindegaard , Ole H. Olsen , Eric Casteleijn , Maarten R. Egmond , Johan Haverkamp , Wouter Musters , Jakob de Vlieg
摘要: This invention relates to enzymes, to rDNA techniques applicable for example to their production, to mutated genes, vectors and mutant and transformed microorganisms useful in their production, and to their uses including for example enzymatic detergent and cleaning compositions containing them.
摘要翻译: 本发明涉及酶,适用于例如其生产的rDNA技术,可用于其生产的突变基因,载体和突变和转化的微生物以及其用途,包括例如含有它们的酶洗涤剂和清洁组合物。
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7.
公开(公告)号:US06632646B1
公开(公告)日:2003-10-14
申请号:US09678867
申请日:2000-10-02
申请人: Dorrit Aaslyng , Sven Branner , Sven Hastrup , Leif Nørskov-Lauritsen , Ole Hvilsted Olsen , Merete Simonsen , Eric Casteleijn , Maarten Robert Egmond , Johan Haverkamp , John David Marugg , Arnoldus Theodorus Anthonius Mooren
发明人: Dorrit Aaslyng , Sven Branner , Sven Hastrup , Leif Nørskov-Lauritsen , Ole Hvilsted Olsen , Merete Simonsen , Eric Casteleijn , Maarten Robert Egmond , Johan Haverkamp , John David Marugg , Arnoldus Theodorus Anthonius Mooren
IPC分类号: C12N950
CPC分类号: C12N9/54 , C11D3/386 , C11D3/38636
摘要: Enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.
摘要翻译: 提出了通过突变多个枯草杆菌蛋白酶的基因并在合适的宿主中表达突变的基因产生的酶。与其野生型亲本酶相比,酶显示改善的洗涤性能。 PTEXT>酶是良好的 - 用于洗涤剂组合物。 PTEXT>
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公开(公告)号:US20050003986A1
公开(公告)日:2005-01-06
申请号:US10896177
申请日:2004-07-21
申请人: Sven Hastrup , Sven Branner , Fanny Norris , Steffen Petersen , Leif Norskov-Lauridsen , Villy Jensen , Dorrit Aaslyng
发明人: Sven Hastrup , Sven Branner , Fanny Norris , Steffen Petersen , Leif Norskov-Lauridsen , Villy Jensen , Dorrit Aaslyng
IPC分类号: C12N15/09 , C11D3/386 , C11D7/42 , C12N1/21 , C12N9/50 , C12N9/54 , C12N9/56 , C12N15/00 , C12N15/57 , C12P21/02 , C12R1/07 , C12R1/125 , C07H21/04 , C12N15/74 , C12Q1/68
摘要: The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.
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9.
公开(公告)号:US07189553B2
公开(公告)日:2007-03-13
申请号:US10314191
申请日:2002-12-06
申请人: Dorrit Aaslyng , Sven Branner , Sven Hastrup , Leif Norskov-Lauritsen , Ole Hvilsted Olsen , Merete Simonsen , Eric Castelijn , Maarten Robert Egmond , Johan Haverkamp , John David Marugg , Arnoldus Theodorus Anthonius Mooren
发明人: Dorrit Aaslyng , Sven Branner , Sven Hastrup , Leif Norskov-Lauritsen , Ole Hvilsted Olsen , Merete Simonsen , Eric Castelijn , Maarten Robert Egmond , Johan Haverkamp , John David Marugg , Arnoldus Theodorus Anthonius Mooren
CPC分类号: C12N9/54 , C11D3/386 , C11D3/38636
摘要: The present invention relates to enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.
摘要翻译: 本发明涉及通过突变多种枯草杆菌蛋白酶的基因并在合适的宿主中表达突变的基因而产生的酶。 与其野生型亲本酶相比,酶显示改善的洗涤性能。 酶非常适合用于洗涤剂组合物中。
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公开(公告)号:US06908991B2
公开(公告)日:2005-06-21
申请号:US10313853
申请日:2002-12-06
申请人: Sven Hastrup , Sven Branner , Fanny Norris , Steffen Bjorn Petersen , Leif Norskov-Lauridsen , Villy Johannes Jensen , Dorrit Aaslyng
发明人: Sven Hastrup , Sven Branner , Fanny Norris , Steffen Bjorn Petersen , Leif Norskov-Lauridsen , Villy Johannes Jensen , Dorrit Aaslyng
IPC分类号: C12N15/09 , C11D3/386 , C11D7/42 , C12N1/21 , C12N9/50 , C12N9/54 , C12N9/56 , C12N15/00 , C12N15/57 , C12P21/02 , C12R1/07 , C12R1/125 , C12N15/75
摘要: The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.
摘要翻译: 本发明涉及导致枯草杆菌蛋白酶的化学特性变化的枯草杆菌蛋白酶基因的突变。 枯草杆菌蛋白酶基因的特异性核酸的突变导致氨基酸取代,从而导致酶功能的改变。 这些突变体酶中的一些显示出对于工业应用有利的物理性质,特别是在洗涤剂工业中,提供对氧化更稳定的枯草杆菌蛋白酶,具有更大的蛋白酶活性,并且表现出改进的可洗性。
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