-
公开(公告)号：US20070141689A1
公开(公告)日：2007-06-21
申请号：US10593164
申请日：2005-03-18
申请人： Swapnil Bhargava , Henrik Bisgard-Frantzen , Henrik Frisner , Anders Vikso-Nielsen , Malcolm Johal
发明人： Swapnil Bhargava , Henrik Bisgard-Frantzen , Henrik Frisner , Anders Vikso-Nielsen , Malcolm Johal
摘要： The present invention relates to method of liquefying starch-containing material, wherein the method comprises the steps of (a) treating the starch-containing material with a bacterial alpha-amylase at a temperature around 70-90° C. for 15-90 minutes, and (b) treating the material obtained in step (a) with an alpha-amylase at a temperature between 60-80° C. for 30-90 minutes. The invention also relates to a process of producing a fermentation product, preferably ethanol, comprising a liquefaction step carried out according to the liquefaction method of the invention.
摘要翻译： 本发明涉及液化含淀粉材料的方法，其中该方法包括以下步骤：（a）在约70-90℃的温度下用细菌α-淀粉酶处理含淀粉材料15-90分钟 ，和（b）用α-淀粉酶在60-80℃的温度下处理步骤（a）中获得的材料30-90分钟。 本发明还涉及一种生产发酵产物，优选乙醇的方法，其包括根据本发明的液化方法进行的液化步骤。
-
公开(公告)号：US20100151549A1
公开(公告)日：2010-06-17
申请号：US12581076
申请日：2009-10-16
IPC分类号： C12P7/06
摘要： The invention relates to a process of producing a fermentation product, such as ethanol, from starch-containing material, including i) subjecting starch-containing material to an alpha-amylase, ii) subjecting the material obtained in step i) to an alpha-glucosidase and/or a maltose-generating enzyme, and iii) fermenting the material in the presence of a fermenting organism, such as yeast. Alternatively the invention relates to a process of producing a fermentation product from starch-containing material, preferably granular starch, which process comprises: a) subjecting starch-containing material to an alpha-glucosidase and optionally a glucose-generating and/or maltose-generating enzyme, and b) fermenting the material in the presence of a fermenting organism.
摘要翻译： 本发明涉及从含淀粉材料生产发酵产物如乙醇的方法，其包括：i）使含淀粉材料经历α-淀粉酶，ii）将步骤i）中得到的材料进行α- 葡萄糖苷酶和/或产生麦芽糖的酶，和iii）在发酵生物体如酵母存在下发酵所述材料。 或者，本发明涉及由含淀粉材料，优选颗粒淀粉生产发酵产物的方法，该方法包括：a）使含淀粉材料经受α-葡糖苷酶和任选地产生葡萄糖和/或产生麦芽糖的物质 酶，和b）在发酵生物存在下发酵该物质。
-
公开(公告)号：US20080032373A1
公开(公告)日：2008-02-07
申请号：US11579748
申请日：2005-05-11
IPC分类号： C12P7/14
摘要： The invention relates to a process of producing a fermentation product, such as ethanol, from starch-containing material, including i) subjecting starch-containing material to an alpha-amylase, ii) subjecting the material obtained in step i) to an alpha-glucosidase and/or a maltose-generating enzyme, and iii) fermenting the material in the presence of a fermenting organism, such as yeast. Alternatively the invention relates to a process of producing a fermentation product from starch-containing material, preferably granular starch, which process comprises: a) subjecting starch-containing material to an alpha-glucosidase and optionally a glucose-generating and/or maltose-generating enzyme, and b) fermenting the material in the presence of a fermenting organism.
摘要翻译： 本发明涉及从含淀粉材料生产发酵产物如乙醇的方法，其包括：i）使含淀粉材料经历α-淀粉酶，ii）将步骤i）中得到的材料进行α- 葡萄糖苷酶和/或产生麦芽糖的酶，和iii）在发酵生物体如酵母存在下发酵所述材料。 或者，本发明涉及由含淀粉材料，优选颗粒淀粉生产发酵产物的方法，该方法包括：a）使含淀粉材料经受α-葡糖苷酶和任选地产生葡萄糖和/或产生麦芽糖的物质 酶，和b）在发酵生物存在下发酵该物质。
-
公开(公告)号：US08263382B2
公开(公告)日：2012-09-11
申请号：US13100407
申请日：2011-05-04
IPC分类号： C12N9/24
CPC分类号： C12N9/2417 , C11D3/386 , C12P19/02 , C12P19/14
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶在下列特性中表现出至少一个变化：底物特异性，底物结合，底物 裂解模式，热稳定性，pH /活性分布，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。
-
公开(公告)号：US08124395B2
公开(公告)日：2012-02-28
申请号：US12952695
申请日：2010-11-23
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
-
公开(公告)号：US07566561B2
公开(公告)日：2009-07-28
申请号：US10327837
申请日：2002-12-23
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译： 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。
-
公开(公告)号：US20070128313A1
公开(公告)日：2007-06-07
申请号：US11633385
申请日：2006-12-04
IPC分类号： A21D8/02 , C12C11/00 , C12P21/06 , C12P19/02 , C12P7/06 , C12N9/32 , C07H21/04 , C12N15/74 , C12N1/16
CPC分类号： C12Y302/01001 , A21D8/042 , C12C5/004 , C12C11/00 , C12N9/2417 , C12N9/242 , C12P7/06 , C12P19/02 , C12P19/14 , C13K1/06 , C13K7/00 , Y02E50/17
摘要： The invention relates to a variant of a parent Fungamyl-like fungal alpha-amylase, which exhibits improved thermal stability at acidic pH suitable for, e.g., starch processes.
摘要翻译： 本发明涉及亲本的Fungamyl样真菌α-淀粉酶的变体，其在适于例如淀粉方法的酸性pH下表现出改进的热稳定性。
-
公开(公告)号：US20050118695A1
公开(公告)日：2005-06-02
申请号：US10980759
申请日：2004-11-03
CPC分类号： C12N9/2417 , C11D3/386 , C12P19/02 , C12P19/14
摘要： The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性，并且相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变：底物特异性，底物结合，底物 裂解模式，热稳定性，pH /活性分布，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。
-
公开(公告)号：US20050059131A1
公开(公告)日：2005-03-17
申请号：US10778469
申请日：2004-02-12
CPC分类号： C11D3/38618 , C11D3/38609 , C11D3/38681 , C12N9/2417 , C12Y302/01001 , D06L1/14
摘要： The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.
摘要翻译： 本发明涉及母体α-淀粉酶的变体，其母体α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.2所示的氨基酸序列的氨基酸序列。 3和SEQ ID No.7; 或（ii）与这些氨基酸序列中的一个或多个显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶产生的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列相同的探针杂交的DNA序列编码; 在该变体中：（a）母体α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）母体α-淀粉酶的至少一个氨基酸残基被不同的氨基酸残基替代; 和/或（c）相对于母体α-淀粉酶插入至少一个氨基酸残基; 该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶表现出以下特性中的至少一个：增加的热稳定性; 提高氧化稳定性; 并减少Ca 2+依赖性; 条件是变体的氨基酸序列分别与SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.7所示的任何氨基酸序列不同。
-
公开(公告)号：US20120283164A1
公开(公告)日：2012-11-08
申请号：US13556623
申请日：2012-07-24
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Neilsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Neilsen , Lisbeth Hedegaard
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译： 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。
-
-
-
-
-
-
-
-
-
搜索结果
45 条记录 (耗时 0.046 秒)