公开(公告)号：US4867850A

公开(公告)日：1989-09-19

申请号：US133057

申请日：1987-12-15

申请人： Syotaro Oka ,  Junichi Kita ,  Hiroki Kuyama ,  Motosada Kiri

发明人： Syotaro Oka ,  Junichi Kita ,  Hiroki Kuyama ,  Motosada Kiri

IPC分类号： G01J5/34 ,  H01L37/02

CPC分类号： G01J5/34 ,  H01L37/02

摘要： Thermal detectors comprising at least one pair of membrane electrodes formed on a base material (such a pyroelectric, semiconductive and insulating materials), wherein at least one member of said pair of electrodes is covered with a black membrane of precious metal formed by the electrolytic process, are highly sensitive because of the excellent heat absorption efficiency, with little change in sensitivity over a wide wavelength range.

公开(公告)号：US07579165B2

公开(公告)日：2009-08-25

申请号：US10962575

申请日：2004-10-13

申请人： Shigemi Norioka ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Takashi Obama ,  Eiji Ando ,  Takashi Nakazawa ,  Norikazu Ueyama ,  Taka-aki Okamura

发明人： Shigemi Norioka ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Takashi Obama ,  Eiji Ando ,  Takashi Nakazawa ,  Norikazu Ueyama ,  Taka-aki Okamura

IPC分类号： C07K1/13 ,  C07K1/14 ,  C07K14/00 ,  C12P21/06 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/37 ,  G01N33/53 ,  G01N33/38 ,  G01N33/483

CPC分类号： C12N9/2462 ,  C07K1/1077 ,  C07K1/128 ,  C07K14/78

摘要： The present invention provides a method for effectively introducing sulfonic acid groups into the N-terminus of a protein or a peptide. The method comprises a modification step to react a N-terminus in a protein or peptide with a compound A which includes disulfide group and a cleavage step to cleave a disulfide bond of the disulfide group to convert into a sulfonic acid group. The present invention also provides a method of analyzing proteins or peptides easily and effectively on mass spectrometry and an intermediate that can be used to effectively derivatize proteins or peptides as sulfonic acid derivatives.

摘要翻译： 本发明提供了将磺酸基团有效地引入蛋白质或肽的N-末端的方法。 该方法包括使蛋白质或肽中的N末端与包含二硫键的化合物A和切割二硫键的二硫键以切割成磺酸基团的切割步骤进行反应的修饰步骤。 本发明还提供了一种在质谱分析中容易且有效地分析蛋白质或肽的方法，以及可用于有效地将蛋白质或肽衍生为磺酸衍生物的中间体。

公开(公告)号：US07294707B2

公开(公告)日：2007-11-13

申请号：US10958298

申请日：2004-10-06

申请人： Takashi Nakazawa ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Norikazu Ueyama ,  Taka-aki Okamura ,  Shigemi Norioka

发明人： Takashi Nakazawa ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Norikazu Ueyama ,  Taka-aki Okamura ,  Shigemi Norioka

IPC分类号： C07K1/00

CPC分类号： C07K1/003

摘要： A simple and low-cost method of selectively modifying the C-terminal of a protein or peptide is provided. A method of modifying the C-terminal of a protein or peptide comprises forming an intramolecular oxazolone ring at the C-terminal of the protein or peptide that requires C-terminal modification, and then performing a ring-opening of the oxazolone ring to produce a protein or peptide with a modified C-terminal. Preferred forms include a method in which by reacting the oxazolone ring with a compound containing a nucleophilic group to effect an oxazolone ring-opening, a protein or peptide is produced in which the C-terminal is modified with the compound containing the nucleophilic group, as well as a method in which by reacting the oxazolone ring with an active esterifying agent to effect a ring-opening, the oxazolone is converted to an active ester, which by subsequent reaction with a compound containing a nucleophilic group, produces a protein or peptide in which the C-terminal has been modified with the compound containing the nucleophilic group.

摘要翻译： 提供了选择性修饰蛋白质或肽的C-末端的简单且低成本的方法。 修饰蛋白质或肽的C末端的方法包括在需要C末端修饰的蛋白质或肽的C-末端形成分子内的恶唑酮环，然后进行恶唑酮环的开环以产生 具有修饰C末端的蛋白质或肽。 优选的形式包括通过使恶唑酮环与含有亲核基团的化合物反应以进行恶唑酮开环的方法，制备其中C-末端用含有亲核基团的化合物修饰的蛋白质或肽作为 以及通过使恶唑酮环与活性酯化剂反应以开环的方法，将恶唑酮转化成活性酯，其随后与含有亲核基团的化合物反应，产生蛋白质或肽 其中C-末端已经与含有亲核基团的化合物一起被修饰。

公开(公告)号：US20060046303A1

公开(公告)日：2006-03-02

申请号：US10531288

申请日：2003-04-03

申请人： Hiroki Kuyama ,  Chikako Toda ,  Osamu Nishimura

发明人： Hiroki Kuyama ,  Chikako Toda ,  Osamu Nishimura

IPC分类号： G01N1/00

CPC分类号： G01N33/6848 ,  C07K1/113 ,  C07K7/06 ,  C07K7/08 ,  G01N33/6842 ,  G01N33/6851 ,  Y10T436/25125

摘要： A method for eliminating a phosphate group of a peptide, the method comprising the use of a reagent containing at least one selected from the group consisting of hydrogen fluoride, hydrofluoric acid, and a hydrogen fluoride-containing compound, and a method for peptide analysis that uses such a method. As the hydrogen fluoride-containing compound, hydrogen fluoride-pyridine is preferably used. The elimination of the phosphate group from a peptide may be carried out so that the total amount of the hydrogen fluoride, hydrogen fluoride in the hydrofluoric acid, and hydrogen fluoride in the hydrogen fluoride-containing compound contain in the reagent is 10 to 100 wt % with respect to the reagent, and the temperature for the elimination reaction is −10 to 50° C.

摘要翻译： 一种消除肽的磷酸基团的方法，该方法包括使用含有选自氟化氢，氢氟酸和含氟化氢的化合物中的至少一种的试剂，以及肽分析方法， 使用这种方法。 作为含氟化氢的化合物，优选使用氟化氢 - 吡啶。 可以进行磷酸基从肽的去除，使得在试剂中含有的氟化氢，氢氟酸中的氟化氢和含氟化氢的化合物中的氟化氢的总量为10〜100重量％ 相对于试剂，消除反应的温度为-10〜50℃。

公开(公告)号：US20050085622A1

公开(公告)日：2005-04-21

申请号：US10958298

申请日：2004-10-06

申请人： Takashi Nakazawa ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Norikazu Ueyama ,  Taka-aki Okamura ,  Shigemi Norioka

发明人： Takashi Nakazawa ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Norikazu Ueyama ,  Taka-aki Okamura ,  Shigemi Norioka

IPC分类号： C07K1/113 ,  C07K1/00 ,  C07K1/08 ,  C07K14/00

CPC分类号： C07K1/003

摘要： A simple and low-cost method of selectively modifying the C-terminal of a protein or peptide is provided. A method of modifying the C-terminal of a protein or peptide comprises forming an intramolecular oxazolone ring at the C-terminal of the protein or peptide that requires C-terminal modification, and then performing a ring-opening of the oxazolone ring to produce a protein or peptide with a modified C-terminal. Preferred forms include a method in which by reacting the oxazolone ring with a compound containing a nucleophilic group to effect an oxazolone ring-opening, a protein or peptide is produced in which the C-terminal is modified with the compound containing the nucleophilic group, as well as a method in which by reacting the oxazolone ring with an active esterifying agent to effect a ring-opening, the oxazolone is converted to an active ester, which by subsequent reaction with a compound containing a nucleophilic group, produces a protein or peptide in which the C-terminal has been modified with the compound containing the nucleophilic group.

摘要翻译： 提供了选择性修饰蛋白质或肽的C-末端的简单且低成本的方法。 修饰蛋白质或肽的C末端的方法包括在需要C末端修饰的蛋白质或肽的C-末端形成分子内的恶唑酮环，然后进行恶唑酮环的开环以产生 具有修饰C末端的蛋白质或肽。 优选的形式包括通过使恶唑酮环与含有亲核基团的化合物反应以进行恶唑酮开环的方法，制备其中C-末端用含有亲核基团的化合物修饰的蛋白质或肽作为 以及通过使恶唑酮环与活性酯化剂反应以开环的方法，将恶唑酮转化成活性酯，其随后与含有亲核基团的化合物反应，产生蛋白质或肽 其中C-末端已经与含有亲核基团的化合物一起被修饰。

公开(公告)号：US5952450A

公开(公告)日：1999-09-14

申请号：US127075

申请日：1998-07-31

申请人： Jiro Ishihara ,  Hiroki Kuyama ,  Eiichi Ozeki ,  Takeshi Ishitoku ,  Masahide Tanaka ,  Naoya Sakamoto

发明人： Jiro Ishihara ,  Hiroki Kuyama ,  Eiichi Ozeki ,  Takeshi Ishitoku ,  Masahide Tanaka ,  Naoya Sakamoto

IPC分类号： C08G64/02 ,  C08G64/22 ,  C08G64/30 ,  C08G64/00

CPC分类号： C08G64/0216 ,  C08G64/226 ,  C08G64/305

摘要： A crosslinked polycarbonate is obtained by polycondensation of diol (A), trivalent or more polyhydric alcohol (B) whose arbitrary two hydroxy groups are not in the positional relationship of 1,2-disubstitution or 1,3-disubstitution, and carbonyl component (C) such as carbonic acid diesters. In the polycondensation step, no side reaction does not occur.This crosslinked polycarbonate is used as a modifier for polylactic acids. The brittleness of the polylactic acid is improved while maintaining mechanical strength, thermostability, and transparency.

摘要翻译： 交联聚碳酸酯通过二醇（A），任意两个羟基不具有1,2-取代或1,3-取代的位置关系的三价以上的多元醇（B）和羰基成分（C ）如碳酸二酯。 在缩聚步骤中，不会发生副反应。 该交联聚碳酸酯用作聚乳酸的改性剂。 改善聚乳酸的脆性，同时保持机械强度，热稳定性和透明度。

公开(公告)号：US07892846B2

公开(公告)日：2011-02-22

申请号：US12269336

申请日：2008-11-12

申请人： Hiroki Kuyama ,  Kazuhiro Sonomura ,  Osamu Nishimura

发明人： Hiroki Kuyama ,  Kazuhiro Sonomura ,  Osamu Nishimura

IPC分类号： G01N33/00

CPC分类号： G01N33/6848 ,  Y10T436/16 ,  Y10T436/24

摘要： According to the present invention, a matrix reagent prepared by adding methylenediphosphonic acid (MDPNA) having two phosphonic acid groups as an additive to a matrix of 2,5-dihydroxybenzoic acid (DHBA) is used for the preparation of a sample. By using the sample according to the present invention, it is possible to achieve a higher peak intensity of phosphopeptide as compared with a sample in which DHBA is solely used as a matrix without using an additive, or a sample in which phosphoric acid (PA) is used as an additive in addition to DHBA. Further, use of the sample according to the present invention enables the detection of peptides that cannot be detected in a case of the phosphoric acid-added sample.

摘要翻译： 根据本发明，通过将具有两个膦酸基的亚甲基二膦酸（MDPNA）作为添加剂添加到2,5-二羟基苯甲酸（DHBA）的基质中而制备的基质试剂用于制备样品。 通过使用根据本发明的样品，与仅使用DHBA作为基质而不使用添加剂的样品相比，可以实现更高的磷酸肽峰值强度，或者其中磷酸（PA） 除了DHBA之外还用作添加剂。 此外，根据本发明的样品的使用能够检测在添加磷酸的样品的情况下不能检测到的肽。

公开(公告)号：US20050224710A1

公开(公告)日：2005-10-13

申请号：US11028216

申请日：2005-01-04

申请人： Eiichi Matsuo ,  Makoto Watanabe ,  Noriyuki Ojima ,  Chikako Toda ,  Hiroki Kuyama ,  Osamu Nishimura

发明人： Eiichi Matsuo ,  Makoto Watanabe ,  Noriyuki Ojima ,  Chikako Toda ,  Hiroki Kuyama ,  Osamu Nishimura

IPC分类号： G01N33/68 ,  H01J49/16

CPC分类号： H01J49/164 ,  G01N33/6848

摘要： The present invention provides a method capable of efficiently ionizing hydrophobic peptides in MALDI-IT, MALDI-IT-TOF, and MALDI-FTICR mass spectrometers. A method of measuring a peptide with a mass spectrometer having a MALDI (Matrix Assisted Laser Desorption/Ionization) ion source, using α-cyano-3-hydroxycinnamic acid or 3-hydroxy-4-nitrobenzoic acid as a matrix. Preferably, a peptide derivatized with 2-nitrobenzenesulfenyl chloride is measured with a MALDI-IT, MALDI-IT-TOF, or MALDI-FTICR mass spectrometer. When 3-hydroxy-4-nitrobenzoic acid is used as a matrix, the matrix is preferably used as a mixed matrix in which α-cyano-4-hydroxycinnamic acid is combined.

摘要翻译： 本发明提供了能够有效地离子化MALDI-IT，MALDI-IT-TOF和MALDI-FTICR质谱仪中的疏水性肽的方法。 使用α-氰基-3-羟基肉桂酸或3-羟基-4-硝基苯甲酸作为基质的具有MALDI（Matrix Assisted Laser Desorption / Ionization）离子源的质谱仪测量肽的方法。 优选地，用MALDI-IT，MALDI-IT-TOF或MALDI-FTICR质谱仪测量用2-硝基苯硫基氯衍生的肽。 当使用3-羟基-4-硝基苯甲酸作为基质时，优选将基质用作混合基质，其中组合了α-氰基-4-羟基肉桂酸。

公开(公告)号：US20050084927A1

公开(公告)日：2005-04-21

申请号：US10962575

申请日：2004-10-13

申请人： Shigemi Norioka ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Takashi Obama ,  Eiji Ando ,  Takashi Nakazawa ,  Norikazu Ueyama ,  Taka-aki Okamura

发明人： Shigemi Norioka ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Takashi Obama ,  Eiji Ando ,  Takashi Nakazawa ,  Norikazu Ueyama ,  Taka-aki Okamura

IPC分类号： C07K1/107 ,  C07K1/12 ,  C07K14/78 ,  C12N9/36 ,  C12P21/06 ,  C07K14/00

CPC分类号： C12N9/2462 ,  C07K1/1077 ,  C07K1/128 ,  C07K14/78

摘要： The present invention provides a method for effectively introducing sulfonic acid groups into the N-terminus of a protein or a peptide; a method capable of analyzing proteins or peptides easily and effectively on mass spectrometry; an intermediate that can be used to effectively derivatize proteins or peptides as sulfonic acid derivatives. A method for derivatizing a protein or peptide to a sulfonic acid derivative, comprising steps of: modification step to react a N-terminus in a protein or peptide with a compound A which includes disulfide group, to obtain a protein or peptide modified with the compound A at the N-terminus; and cleavage step to cleave a disulfide bond of the disulfide group to convert into a sulfonic acid group, thereby converting the modified protein or peptide into a sulfonic acid derivative. A method for analyzing the amino acid sequence of a protein or peptide, wherein the sulfonic acid derivative of a protein or a peptide obtained by the above method is subjected to mass spectrometry. A protein or peptide modified with a disulfide group-containing group at the N-terminus.

摘要翻译： 本发明提供了有效地将磺酸基引入蛋白质或肽的N末端的方法; 能够在质谱上容易且有效地分析蛋白质或肽的方法; 可用于有效地将蛋白质或肽衍生为磺酸衍生物的中间体。 一种将蛋白质或肽衍生为磺酸衍生物的方法，包括以下步骤：使蛋白质或肽中的N末端与包含二硫键的化合物A反应的修饰步骤，以获得用化合物修饰的蛋白质或肽 A在N端; 以及切割二硫键的二硫键转化为磺酸基的切割步骤，从而将修饰的蛋白质或肽转化为磺酸衍生物。 分析蛋白质或肽的氨基酸序列的方法，其中通过上述方法获得的蛋白质或肽的磺酸衍生物进行质谱分析。 在N末端用含二硫基的基团修饰的蛋白质或肽。

公开(公告)号：US20090325227A1

公开(公告)日：2009-12-31

申请号：US12090747

申请日：2006-10-23

申请人： Takashi Nakazawa ,  Mutsumi Oka ,  Kimiko Nishida ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Norikazu Ueyama ,  Takaaki Okamura ,  Susumu Tsunasawa

发明人： Takashi Nakazawa ,  Mutsumi Oka ,  Kimiko Nishida ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Norikazu Ueyama ,  Takaaki Okamura ,  Susumu Tsunasawa

IPC分类号： C12P21/00 ,  C07K1/107 ,  G01N33/68

CPC分类号： C07K17/00 ,  C07K1/1072 ,  C07K1/113

摘要： The present invention provides a method for inexpensively, easily and efficiently modifying the C-terminus of a protein or peptide; a method for easily and reliably isolating a C-terminal peptide fragment of a protein or peptide; and a method for rapidly, accurately and reliably determining an amino acid sequence of a protein or peptide by using a mass spectroscope. A method comprising the step of adding a formylation reagent and a catalyst to a protein or peptide to convert a carboxyl group into an aldehyde group. A method comprising the step of reacting a nucleophilic reagent with the aldehyde group to modify the C-terminus of the protein or peptide. A method comprising the step of reacting a support having a nucleophilic group to immobilize the protein or peptide. A method comprising the step of fragmenting the immobilized protein or peptide, washing the support, and isolating the C-terminal peptide fragment from the support. A method comprising the step of subjecting the isolated C-terminal peptide fragment to mass spectrometry and determining an amino acid sequence.

摘要翻译： 本发明提供了一种廉价，容易且有效地修饰蛋白质或肽的C末端的方法; 容易且可靠地分离蛋白质或肽的C末端肽片段的方法; 以及通过使用质谱仪快速，准确，可靠地测定蛋白质或肽的氨基酸序列的方法。 一种方法，其包括将甲酰化试剂和催化剂加入到蛋白质或肽中以将羧基转化为醛基的步骤。 一种包括使亲核试剂与醛基反应以修饰蛋白质或肽的C末端的步骤的方法。 一种包括使具有亲核基团的载体反应以固定蛋白质或肽的方法。 一种方法，其包括将固定的蛋白质或肽片段化，洗涤载体和从载体中分离C末端肽片段的步骤。 一种方法，包括使分离的C-末端肽片段进行质谱分析和测定氨基酸序列的步骤。