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1.发明申请REDUCTION OF CARBON DIOXIDE EMISSION DURING ISOPRENE PRODUCTION BY FERMENTATION 审中-公开通过发酵减少异丙酚生产过程中的二氧化碳排放公开(公告)号：US20130280778A1
公开(公告)日：2013-10-24
申请号：US13899419
申请日：2013-05-21
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , C12N1/20 , Y02E50/343
摘要： The present invention provides methods for increasing the amount of isoprene produced by cultured cells with only a minimal increase in carbon dioxide emitted, thereby resulting in process having a greater yield of isoprene relative to carbon dioxide. In addition, the present invention provides compositions that include the cultured cells or isoprene produced there from.
摘要翻译： 本发明提供了增加培养细胞产生的异戊二烯量的方法，其中发射的二氧化碳的增加只有极小的增加，从而导致异戊二烯相对于二氧化碳的产量更高的方法。 此外，本发明提供了包含从其制备的培养细胞或异戊二烯的组合物。
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公开(公告)号：US20180066287A1
公开(公告)日：2018-03-08
申请号：US15677335
申请日：2017-08-15
发明人： Anthony R. CALABRIA , Marguerite A. CERVIN , Gopal K. CHOTANI , Richard LA DUCA , Joseph C. MCAULIFFE , Michael C. MILLER , Timothy A. SABO , Karl J. SANFORD , Erin L. Spring , Gregory M. WHITED
IPC分类号： C12P5/00 , C12N15/80 , C12N15/75 , C12N15/74 , C12N15/70 , C12N15/52 , C07C11/18 , C12P5/02 , C12N9/88
CPC分类号： C12P5/007 , C07C11/18 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/75 , C12N15/80 , C12P5/02 , C12Y402/03027 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.
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公开(公告)号：US20150031105A1
公开(公告)日：2015-01-29
申请号：US14295184
申请日：2014-06-03
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , Y02E50/343
摘要： The invention provides improved methods for the production of isoprene from biological materials.
摘要翻译： 本发明提供从生物材料生产异戊二烯的改进方法。
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4.发明申请COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE FREE OF C5 HYDROCARBONS UNDER DECOUPLING CONDITIONS AND/OR SAFE OPERATING RANGES 审中-公开在解除条件和/或安全操作范围内生产不含C5碳氢化合物的异丙苯的组合物和方法公开(公告)号：US20160281112A1
公开(公告)日：2016-09-29
申请号：US14973485
申请日：2015-12-17
发明人： Anthony R. CALABRIA , Marguerite A. CERVIN , Gopal K. CHOTANI , Richard LA DUCA , Joseph C. MCAULIFFE , Michael C. MILLER , Timothy A. SABO , Karl J. SANFORD , Erin L. WEBSTER , Gregory M. WHITED
CPC分类号： C12P5/007 , C07C11/18 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/75 , C12N15/80 , C12P5/02 , C12Y402/03027 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a nonflammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.
摘要翻译： 本发明的特征在于从培养细胞产生异戊二烯的方法，其中固定相中的细胞。 本发明还提供包含这些培养细胞和/或增加量的异戊二烯的组合物。 本发明还提供了在气相中包含不可燃浓度的异戊二烯的体系。 此外，本发明提供了异戊二烯组合物，例如具有增加量的异戊二烯或增加的纯度的组合物。
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公开(公告)号：US20180273982A1
公开(公告)日：2018-09-27
申请号：US15873761
申请日：2018-01-17
发明人： Marguerite A. CERVIN , Gopal K. CHOTANI , Frank J. FEHER , Richard LA DUCA , Joseph C. MCAULIFFE , Andrei MIASNIKOV , Caroline M. PERES , Aaron S. PUHALA , Karl J. SANFORD , Fernando VALLE , Gregory M. WHITED
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
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公开(公告)号：US20140273145A1
公开(公告)日：2014-09-18
申请号：US14218720
申请日：2014-03-18
发明人： Martien H. BERGSMA , Anthony R. CALABRIA , Gopal K. CHOTANI , William A. CUEVAS , Gang DUAN , Sung Ho LEE , Ying QIAN , Vivek SHARMA , Jayarama K. SHETTY , Bruce A. STROHM , Paula Johanna Maria TEUNISSEN , Hongxian XU
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , C12N9/2428 , C12P5/026 , C12P19/02 , C12P19/14 , C12Y302/01003
摘要： Embodiments of the present disclosure relate to a process for producing isoprene from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。
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公开(公告)号：US20150004665A1
公开(公告)日：2015-01-01
申请号：US14485314
申请日：2014-09-12
发明人： Gopal K. CHOTANI , Brian KIRSHNER , Jacob LATONE , Jeff P. PUCCI
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , C12N9/88 , C12Y402/03027
摘要： This invention relates to methods for producing isoprene by culturing recombinant cells (e.g., cells engineered to produce isoprene) under reduced oxygen inlet levels.
摘要翻译： 本发明涉及通过在减少的氧气入口水平下培养重组细胞（例如，改造成产生异戊二烯的细胞）来生产异戊二烯的方法。
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8.发明申请METHODS FOR INCREASING MICROBIAL PRODUCTION OF ISOPRENE, ISOPRENOIDS, AND ISOPRENOID PRECURSOR MOLECULES USING GLUCOSE AND ACETATE CO-METABOLISM 审中-公开使用葡萄糖和乙酸盐代谢来增加异丙肾上腺素，异丙肾上腺素和异丙肾上腺素分子的微生物生产的方法公开(公告)号：US20140335576A1
公开(公告)日：2014-11-13
申请号：US14279053
申请日：2014-05-15
摘要： Provided herein are methods for the increased production of intracellular acetyl-CoA, mevalonate, isoprenoid precursors, isoprene and/or isoprenoids by recombinant microorganisms via co-metabolism of substrates with varied oxidation levels.
摘要翻译： 本文提供了通过重组微生物通过具有不同氧化水平的底物的共代谢增加细胞内乙酰辅酶A，甲羟戊酸，类异戊二烯前体，异戊二烯和/或类异戊二烯的生产的方法。
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公开(公告)号：US20140162337A1
公开(公告)日：2014-06-12
申请号：US13940092
申请日：2013-07-11
发明人： Gopal K. CHOTANI , Joseph C. MCAULIFFE , Michael C. MILLER , Rachel E. MUIR , Dmitrii V. VAVILINE , Walter WEYLER
摘要： The invention provides for methods for producing isoprene from cultured cells using various components of the DXP pathway and MVA pathway, or components associated with the DXP pathway and MVA pathway, iron-sulfur cluster-interacting redox polypeptides, and isoprene synthase. The invention also provides compositions that include these cultured cells.
摘要翻译： 本发明提供了使用DXP途径和MVA途径的各种组分或与DXP途径和MVA途径相关的组分，铁 - 硫簇相互作用的氧化还原多肽和异戊二烯合酶从培养细胞产生异戊二烯的方法。 本发明还提供包括这些培养细胞的组合物。
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公开(公告)号：US20140127770A1
公开(公告)日：2014-05-08
申请号：US14148332
申请日：2014-01-06
IPC分类号： C12P5/00
摘要： The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.
摘要翻译： 本发明的特征在于从培养细胞产生异戊二烯和共产物如乙醇，1,3-丙二醇或氢的方法。 本发明还提供包括这些培养细胞的组合物。 本发明提供了包含异戊二烯和乙醇，异戊二烯和1,3-丙二醇以及异戊二烯和氢的组合物。 另外，本发明提供了通过在适合共同生产异戊二烯和乙醇，异戊二烯和1,3-丙二醇的条件下培养细胞来共同生产异戊二烯和乙醇，异戊二烯和1,3-丙二醇以及异戊二烯和氢的方法，以及 异戊二烯和氢。
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