公开(公告)号：US11564639B2

公开(公告)日：2023-01-31

申请号：US14767836

申请日：2014-02-13

Applicant: The Trustees of Dartmouth College

Inventor： Kenneth Tichauer ,  Robert W. Holt ,  Frederic Leblond ,  Pablo Valdes ,  Brian W. Pogue ,  Keith D. Paulsen ,  David W. Roberts

IPC: A61B5/00 ,  A61K49/00

Abstract: A method of generating corrected fluorescence data of concentrations of a targeted fluorophore in tissue of a subject includes administering first and second fluorescent contrast agents to the subject, the first contrast agent targeted to tissue of interest, the second agent untargeted. The tissue is illuminated with light of a first stimulus wavelength and first data is acquired at an appropriate emissions wavelength; the tissue is illuminated at a second stimulus wavelength and second data is acquired at a second emissions wavelength associated with the second agent, the first and second emissions wavelength differ. Difference data is generated by subtracting the second data from the first data. A system provides for stimulus and capture at multiple wavelengths, with image storage memory and subtraction code, to perform the method. Corrected data may form an fluorescence image, or is used to generate fluorescence tomographic images.

公开(公告)号：US20150374308A1

公开(公告)日：2015-12-31

申请号：US14767836

申请日：2014-02-13

Applicant: THE TRUSTEES OF DARTMOUTH COLLEGE

Inventor： Kenneth Tichauer ,  Robert W. Holt ,  Frederic Leblond ,  Pablo Valdes ,  Brian W. Pogue ,  Keith D. Paulsen ,  David W. Roberts

IPC: A61B5/00 ,  A61K49/00

Abstract: A method of generating corrected fluorescence data of concentrations of a targeted fluorophore in tissue of a subject includes administering first and second fluorescent contrast agents to the subject, the first contrast agent targeted to tissue of interest, the second agent untargeted. The tissue is illuminated with light of a first stimulus wavelength and first data is acquired at an appropriate emissions wavelength; the tissue is illuminated at a second stimulus wavelength and second data is acquired at a second emissions wavelength associated with the second agent, the first and second emissions wavelength differ. Difference data is generated by subtracting the second data from the first data. A system provides for stimulus and capture at multiple wavelengths, with image storage memory and subtraction code, to perform the method. Corrected data may form an fluorescence image, or is used to generate fluorescence tomographic images.

Abstract translation: 产生受试者组织中靶向荧光团浓度的校正荧光数据的方法包括向受试者施用第一和第二荧光造影剂，靶向目的组织的第一造影剂，第二剂未被靶向。 用第一刺激波长的光照射组织，并以适当的发射波长获取第一数据; 在第二刺激波长照射组织，并且以与第二试剂相关联的第二发射波长获取第二数据，第一和第二发射波长不同。 通过从第一数据中减去第二数据来产生差分数据。 系统提供多个波长的刺激和捕获，具有图像存储和减法代码，以执行该方法。 校正的数据可以形成荧光图像，或用于产生荧光断层图像。

公开(公告)号：US11937951B2

公开(公告)日：2024-03-26

申请号：US18159495

申请日：2023-01-25

Applicant: The Trustees of Dartmouth College

Inventor： Kenneth Tichauer ,  Robert W. Holt ,  Frederic Leblond ,  Pablo Valdes ,  Brian W. Pogue ,  Keith D. Paulsen ,  David W. Roberts

IPC: A61B5/00 ,  A61K49/00

CPC classification number: A61B5/7278 ,  A61B5/0071 ,  A61K49/0058 ,  A61B5/0035 ,  A61B5/0062 ,  A61B2576/00

Abstract: A method of generating corrected fluorescence data of concentrations of a targeted fluorophore in tissue of a subject includes administering first and second fluorescent contrast agents to the subject, the first contrast agent targeted to tissue of interest, the second agent untargeted. The tissue is illuminated with light of a first stimulus wavelength and first data is acquired at an appropriate emissions wavelength; the tissue is illuminated at a second stimulus wavelength and second data is acquired at a second emissions wavelength associated with the second agent, the first and second emissions wavelength differ. Difference data is generated by subtracting the second data from the first data. A system provides for stimulus and capture at multiple wavelengths, with image storage memory and subtraction code, to perform the method. Corrected data may form an fluorescence image, or is used to generate fluorescence tomographic images.

公开(公告)号：US20230233157A1

公开(公告)日：2023-07-27

申请号：US18159495

申请日：2023-01-25

Applicant: The Trustees of Dartmouth College

Inventor： Kenneth Tichauer ,  Robert W. Holt ,  Frederic Leblond ,  Pablo Valdes ,  Brian W. Pogue ,  Keith D. Paulsen ,  David W. Roberts

IPC: A61B5/00 ,  A61K49/00

CPC classification number: A61B5/7278 ,  A61B5/0071 ,  A61K49/0058 ,  A61B5/0062 ,  A61B5/0035 ,  A61B2576/00

Abstract: A method of generating corrected fluorescence data of concentrations of a targeted fluorophore in tissue of a subject includes administering first and second fluorescent contrast agents to the subject, the first contrast agent targeted to tissue of interest, the second agent untargeted. The tissue is illuminated with light of a first stimulus wavelength and first data is acquired at an appropriate emissions wavelength; the tissue is illuminated at a second stimulus wavelength and second data is acquired at a second emissions wavelength associated with the second agent, the first and second emissions wavelength differ. Difference data is generated by subtracting the second data from the first data. A system provides for stimulus and capture at multiple wavelengths, with image storage memory and subtraction code, to perform the method. Corrected data may form an fluorescence image, or is used to generate fluorescence tomographic images.

公开(公告)号：US20160278678A1

公开(公告)日：2016-09-29

申请号：US15044097

申请日：2016-02-15

Applicant: The Trustees of Dartmouth College

Inventor： Pablo Valdes ,  Frederic Leblone ,  Keith D. Paulsen ,  Brian Campbell Wilson ,  David W. Roberts ,  Michael Jermyn

IPC: A61B5/1455 ,  A61B5/00 ,  A61B1/04 ,  A61B5/055 ,  A61B5/1495 ,  A61B1/06 ,  A61B1/00 ,  A61B5/1459 ,  A61B5/145

CPC classification number: A61B5/14556 ,  A61B1/00009 ,  A61B1/00165 ,  A61B1/00193 ,  A61B1/042 ,  A61B1/043 ,  A61B1/05 ,  A61B1/0646 ,  A61B1/0669 ,  A61B1/0684 ,  A61B1/07 ,  A61B1/3132 ,  A61B5/0071 ,  A61B5/0077 ,  A61B5/055 ,  A61B5/14546 ,  A61B5/14553 ,  A61B5/1459 ,  A61B5/1495 ,  A61B5/4836 ,  A61B5/7264 ,  G01J3/0229 ,  G01J3/1256 ,  G01J3/2823 ,  G01J2003/1269 ,  G01J2003/2826

Abstract: An imaging system, such as a surgical microscope, laparoscope, or endoscope or integrated with these devices, includes an illuminator providing patterned white light and/or fluorescent stimulus light. The system receives and images light hyperspectrally, in embodiments using a hyperspectral imaging array, and/or using narrowband tunable filters for passing filtered received light to an imager. Embodiments may construct a 3-D surface model from stereo images, and will estimate optical properties of the target using images taken in patterned light or using other approximations obtained from white light exposures. Hyperspectral images taken under stimulus light are displayed as fluorescent images, and corrected for optical properties of tissue to provide quantitative maps of fluorophore concentration. Spectral information from hyperspectral images is processed to provide depth of fluorophore below the tissue surface. Quantitative images of fluorescence at depth are also prepared. The images are displayed to a surgeon for use in surgery.

Abstract translation: 诸如手术显微镜，腹腔镜或内窥镜或与这些装置集成的成像系统包括提供图案化的白光和/或荧光激发光的照明器。 在使用高光谱成像阵列的实施例中，系统接收并高光图像光，并且/或使用窄带可调滤波器将滤波的接收光传递到成像器。 实施例可以从立体图像构造3-D表面模型，并且将使用在图案化光线中拍摄的图像或使用从白光曝光获得的其它近似来估计目标的光学性质。 在激发光下拍摄的高光谱图像显示为荧光图像，并对组织的光学性质进行校正，以提供荧光团浓度的定量图谱。 处理来自高光谱图像的光谱信息，以提供组织表面下方的荧光团的深度。 还准备了深度荧光的定量图像。 图像显示给外科医生用于手术。

公开(公告)号：US20240148291A1

公开(公告)日：2024-05-09

申请号：US18401664

申请日：2024-01-01

Applicant: The Trustees of Dartmouth College

Inventor： Pablo Valdes ,  Frederic Leblond ,  Keith D. Paulsen ,  Brian Campbell Wilson ,  David W. Roberts ,  Michael Jermyn

IPC: A61B5/1455 ,  A61B1/00 ,  A61B1/04 ,  A61B1/05 ,  A61B1/06 ,  A61B1/07 ,  A61B5/00 ,  A61B5/055 ,  A61B5/145 ,  A61B5/1459 ,  A61B5/1495 ,  G01J3/02 ,  G01J3/12 ,  G01J3/28

CPC classification number: A61B5/14556 ,  A61B1/00009 ,  A61B1/000094 ,  A61B1/00165 ,  A61B1/00193 ,  A61B1/00194 ,  A61B1/042 ,  A61B1/043 ,  A61B1/05 ,  A61B1/0646 ,  A61B1/0669 ,  A61B1/0684 ,  A61B1/07 ,  A61B5/0071 ,  A61B5/0077 ,  A61B5/055 ,  A61B5/14546 ,  A61B5/14553 ,  A61B5/1459 ,  A61B5/1495 ,  A61B5/7264 ,  G01J3/0229 ,  G01J3/1256 ,  G01J3/2823 ,  A61B1/3132

Abstract: An imaging system, such as a surgical microscope, laparoscope, or endoscope or integrated with these devices, includes an illuminator providing patterned white light and/or fluorescent stimulus light. The system receives and images light hyperspectrally, in embodiments using a hyperspectral imaging array, and/or using narrowband tunable filters for passing filtered received light to an imager. Embodiments may construct a 3-D surface model from stereo images, and will estimate optical properties of the target using images taken in patterned light or using other approximations obtained from white light exposures. Hyperspectral images taken under stimulus light are displayed as fluorescent images, and corrected for optical properties of tissue to provide quantitative maps of fluorophore concentration. Spectral information from hyperspectral images is processed to provide depth of fluorophore below the tissue surface. Quantitative images of fluorescence at depth are also prepared. The images are displayed to a surgeon for use in surgery.

公开(公告)号：US11857317B2

公开(公告)日：2024-01-02

申请号：US17961480

申请日：2022-10-06

Applicant: The Trustees of Dartmouth College

Inventor： Pablo Valdes ,  Frederic Leblond ,  Keith D. Paulsen ,  Brian Campbell Wilson ,  David W. Roberts ,  Michael Jermyn

IPC: A61B5/1455 ,  A61B5/1459 ,  A61B1/04 ,  A61B5/145 ,  A61B5/00 ,  A61B5/1495 ,  A61B1/06 ,  A61B5/055 ,  G01J3/28 ,  A61B1/05 ,  G01J3/12 ,  G01J3/02 ,  A61B1/00 ,  A61B1/07 ,  A61B1/313

CPC classification number: A61B5/14556 ,  A61B1/00009 ,  A61B1/000094 ,  A61B1/00165 ,  A61B1/00193 ,  A61B1/00194 ,  A61B1/042 ,  A61B1/043 ,  A61B1/05 ,  A61B1/0646 ,  A61B1/0669 ,  A61B1/0684 ,  A61B1/07 ,  A61B5/0071 ,  A61B5/0077 ,  A61B5/055 ,  A61B5/1459 ,  A61B5/1495 ,  A61B5/14546 ,  A61B5/14553 ,  A61B5/7264 ,  G01J3/0229 ,  G01J3/1256 ,  G01J3/2823 ,  A61B1/3132 ,  G01J2003/1269 ,  G01J2003/2826

Abstract: An imaging system, such as a surgical microscope, laparoscope, or endoscope or integrated with these devices, includes an illuminator providing patterned white light and/or fluorescent stimulus light. The system receives and images light hyperspectrally, in embodiments using a hyperspectral imaging array, and/or using narrowband tunable filters for passing filtered received light to an imager. Embodiments may construct a 3-D surface model from stereo images, and will estimate optical properties of the target using images taken in patterned light or using other approximations obtained from white light exposures. Hyperspectral images taken under stimulus light are displayed as fluorescent images, and corrected for optical properties of tissue to provide quantitative maps of fluorophore concentration. Spectral information from hyperspectral images is processed to provide depth of fluorophore below the tissue surface. Quantitative images of fluorescence at depth are also prepared. The images are displayed to a surgeon for use in surgery.

公开(公告)号：US11510600B2

公开(公告)日：2022-11-29

申请号：US15044097

申请日：2016-02-15

Applicant: The Trustees of Dartmouth College

Inventor： Pablo Valdes ,  Frederic Leblond ,  Keith D. Paulsen ,  Brian Campbell Wilson ,  David W. Roberts ,  Michael Jermyn

IPC: A61B5/1455 ,  A61B5/1459 ,  A61B1/04 ,  A61B5/145 ,  A61B5/00 ,  A61B5/1495 ,  A61B1/06 ,  A61B1/00 ,  A61B5/055 ,  G01J3/28 ,  A61B1/05 ,  G01J3/12 ,  G01J3/02 ,  A61B1/07 ,  A61B1/313

Abstract: An imaging system, such as a surgical microscope, laparoscope, or endoscope or integrated with these devices, includes an illuminator providing patterned white light and/or fluorescent stimulus light. The system receives and images light hyperspectrally, in embodiments using a hyperspectral imaging array, and/or using narrowband tunable filters for passing filtered received light to an imager. Embodiments may construct a 3-D surface model from stereo images, and will estimate optical properties of the target using images taken in patterned light or using other approximations obtained from white light exposures. Hyperspectral images taken under stimulus light are displayed as fluorescent images, and corrected for optical properties of tissue to provide quantitative maps of fluorophore concentration. Spectral information from hyperspectral images is processed to provide depth of fluorophore below the tissue surface. Quantitative images of fluorescence at depth are also prepared. The images are displayed to a surgeon for use in surgery.

公开(公告)号：US12285277B2

公开(公告)日：2025-04-29

申请号：US18614944

申请日：2024-03-25

Applicant: The Trustees of Dartmouth College

Inventor： Kenneth Tichauer ,  Robert W. Holt ,  Frederic Leblond ,  Pablo Valdes ,  Brian W. Pogue ,  Keith D. Paulsen ,  David W. Roberts

IPC: A61B5/00 ,  A61K49/00

Abstract: A method of generating corrected fluorescence data of concentrations of a targeted fluorophore in tissue of a subject includes administering first and second fluorescent contrast agents to the subject, the first contrast agent targeted to tissue of interest, the second agent untargeted. The tissue is illuminated with light of a first stimulus wavelength and first data is acquired at an appropriate emissions wavelength; the tissue is illuminated at a second stimulus wavelength and second data is acquired at a second emissions wavelength associated with the second agent, the first and second emissions wavelength differ. Difference data is generated by subtracting the second data from the first data. A system provides for stimulus and capture at multiple wavelengths, with image storage memory and subtraction code, to perform the method. Corrected data may form an fluorescence image, or is used to generate fluorescence tomographic images.

公开(公告)号：US20240260909A1

公开(公告)日：2024-08-08

申请号：US18614944

申请日：2024-03-25

Applicant: The Trustees of Dartmouth College

Inventor： Kenneth Tichauer ,  Robert W. Holt ,  Frederic Leblond ,  Pablo Valdes ,  Brian W. Pogue ,  Keith D. Paulsen ,  David W. Roberts

IPC: A61B5/00 ,  A61K49/00

CPC classification number: A61B5/7278 ,  A61B5/0071 ,  A61K49/0058 ,  A61B5/0035 ,  A61B5/0062 ,  A61B2576/00

Abstract: A method of generating corrected fluorescence data of concentrations of a targeted fluorophore in tissue of a subject includes administering first and second fluorescent contrast agents to the subject, the first contrast agent targeted to tissue of interest, the second agent untargeted. The tissue is illuminated with light of a first stimulus wavelength and first data is acquired at an appropriate emissions wavelength; the tissue is illuminated at a second stimulus wavelength and second data is acquired at a second emissions wavelength associated with the second agent, the first and second emissions wavelength differ. Difference data is generated by subtracting the second data from the first data. A system provides for stimulus and capture at multiple wavelengths, with image storage memory and subtraction code, to perform the method. Corrected data may form an fluorescence image, or is used to generate fluorescence tomographic images.