公开(公告)号：US20110143336A1

公开(公告)日：2011-06-16

申请号：US12996908

申请日：2008-10-10

申请人： Takashi Imai ,  Mayumi Iwakawa ,  Shingo Kato ,  Tatsuya Ohno

发明人： Takashi Imai ,  Mayumi Iwakawa ,  Shingo Kato ,  Tatsuya Ohno

IPC分类号： C12Q1/70 ,  C12Q1/68 ,  G01N33/53

CPC分类号： G01N33/57442 ,  G01N33/57411 ,  G01N2333/025 ,  G01N2800/52 ,  G01N2800/56

摘要： To provide a novel biomarker for estimating the diagnosis of cervical adenocarcinoma or for estimating the prognosis of cervical cancer. An antibody against Villin 1 is employed as a biomarker.

摘要翻译： 提供一种用于估计宫颈腺癌诊断或估计子宫颈癌预后的新型生物标志物。 使用抗Villin 1的抗体作为生物标志物。

公开(公告)号：US20100130376A1

公开(公告)日：2010-05-27

申请号：US12452500

申请日：2008-07-07

申请人： Takashi Imai ,  Mayumi Iwakawa ,  Eisei Oda

发明人： Takashi Imai ,  Mayumi Iwakawa ,  Eisei Oda

IPC分类号： C40B30/04 ,  C40B40/08

CPC分类号： C12Q1/6883 ,  C12Q1/6837 ,  C12Q2600/106 ,  C12Q2600/142 ,  C12Q2600/156

摘要： A DNA chip and a prediction method for predicting the occurrence of a late adverse reaction in a urinary organ after C-ion RT are provided. The DNA chip comprises a supporting means for supporting a DNA probe thereon, and a plurality of genetic markers supported on the supporting means. The prediction method comprises a first step of hybridizing a genetic marker with a labeled DNA prepared from a subject to be examined, a second step of identifying bases of both alleles of the labeled DNA hybridized with the genetic marker, and a third step of determining a genotype of the labeled DNA as a risk genotype if the combination of the identified bases corresponds to the specified combination, and predicting that the subject is predisposed to develop a late adverse reaction in a urinary organ after radiotherapy when the number of the risk genotypes is three or more and the subject is not predisposed to develop a late adverse reaction in a urinary organ after radiotherapy when the number of the risk genotypes is two or less. The method enables to predict whether or not a subject is affected with a late adverse reaction in a urinary organ after radiotherapy.

摘要翻译： 提供DNA芯片和用于预测C-ion RT后的尿器官晚期不良反应发生的预测方法。 DNA芯片包括用于在其上支撑DNA探针的支撑装置和支撑在支撑装置上的多个遗传标记。 该预测方法包括：将遗传标记与由待检测对象制备的标记DNA杂交的第一步骤，鉴定与遗传标记杂交的标记DNA的两个等位基因的碱基的第二步骤，以及第三步骤， 如果所鉴定的碱基的组合对应于指定的组合，则标记的DNA的基因型为风险基因型，并且当风险基因型的数量为3时，预测该受试者易于在放射治疗后在尿器官中发展晚期不良反应 或以上，并且当风险基因型的数目为2或更小时，该受试者在放射治疗后不容易发生尿脏器官晚期不良反应。 该方法能够预测放射治疗后尿液器官的晚期不良反应是否影响受试者。

公开(公告)号：US20090311753A1

公开(公告)日：2009-12-17

申请号：US12214044

申请日：2008-06-16

申请人： Takashi Imai ,  Mayumi Iwakawa ,  Yuichi Michikawa

发明人： Takashi Imai ,  Mayumi Iwakawa ,  Yuichi Michikawa

IPC分类号： C12P19/34

CPC分类号： C12Q1/6846 ,  C12Q2565/518 ,  C12Q2531/119 ,  C12Q2527/119 ,  C12Q2527/101

摘要： A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.

摘要翻译： 提供了扩增基因组DNA的方法。 该方法包括以下步骤：（1）在9至12的pH和45至80℃的温度下培养含细胞的琼脂糖溶液以产生基因组DNA分散的琼脂糖溶液，其中0.002-1拷贝/ 5 单链基因组DNA的微升分散; （2）固定基因组DNA分散的琼脂糖溶液以产生基因组DNA分散的琼脂糖凝胶并中和凝胶的pH; 和（3）将具有链置换活性的DNA聚合酶，引物和dNTP加入到基因组DNA分散的琼脂糖凝胶中，并在0至60℃的温度下孵育该凝胶以扩增基因组DNA。

公开(公告)号：US20070264648A1

公开(公告)日：2007-11-15

申请号：US11660658

申请日：2005-09-22

申请人： Takashi Imai ,  Yoshinobu Harada ,  Mayumi Iwakawa

发明人： Takashi Imai ,  Yoshinobu Harada ,  Mayumi Iwakawa

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： C12Q1/6881 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156

摘要： There are provided a DNA oligomer, a genetic marker, and a DNA oligomer set (PCR primer set) and a DNA oligomer (extension primer) for predicting a possibility of onset of a side-effect from radiation therapy for cancer by determining whether a specific base in a DNA sequence is a risk allele or a non-risk allele, and a method for predicting onset of a side-effect from radiation therapy. The DNA oligomer for a prediction of onset of a side-effect from radiation therapy has a DNA sequence of at least 10-241 contiguous bases with a 121st base from a sequence of any one of SEQ ID NOs: 1-173 in the Sequence Listing.

摘要翻译： 提供DNA寡聚体，遗传标记和DNA寡聚体（PCR引物组）和DNA寡聚物（延伸引物），用于通过确定是否具体的特异性DNA来预测癌症放射治疗副作用的可能性 DNA序列中的碱基是风险等位基因或非风险等位基因，以及预测放射治疗副作用发生的方法。 用于预测放射治疗副作用发作的DNA寡聚体具有至少10-241个连续碱基的DNA序列，其中序列表中SEQ ID NO：1-173中任一个的序列具有121个碱基 。

公开(公告)号：US20090317813A1

公开(公告)日：2009-12-24

申请号：US12310331

申请日：2007-03-30

申请人： Takashi Imai ,  Mayumi Iwakawa ,  Hitoshi Shibuya ,  Masahiko Miura ,  Ryoichi Yoshimura ,  Hiroshi Watanabe

发明人： Takashi Imai ,  Mayumi Iwakawa ,  Hitoshi Shibuya ,  Masahiko Miura ,  Ryoichi Yoshimura ,  Hiroshi Watanabe

IPC分类号： C12Q1/68

CPC分类号： G01N33/57407 ,  C12Q1/6886 ,  C12Q2600/118 ,  G01N2333/705 ,  G01N2333/71

摘要： A method for objectively predicting possibility of metastasis to a cervical lymph node in an early stage for an individual case diagnosed as an oral cavity cancer, and a diagnosis kit to be used in the prediction are provided. The method includes a step of assaying expression amounts of metastasis prediction genes in which the expression amounts are changed between a metastasis group and a non-metastasis group, with respect to a sample collected from a primary legion of the oral cavity cancer. Further, the method includes a step of predicting the possibility of the metastasis by comparing the expression amounts of the metastasis gene group with the expression amounts of the metastasis prediction genes in a metastasis group and/or a non-metastasis group. Herein, the metastasis prediction gene group includes two genes MSR1 (NM_138716.1) and RET (M31213.1).

摘要翻译： 提供了用于客观预测诊断为口腔癌的个别病例的早期颈部淋巴结转移可能性的方法，以及用于预测的诊断试剂盒。 该方法包括相对于从口腔癌的主要团队收集的样品，测定转移组和非转移组之间的表达量发生变化的转移预测基因的表达量的步骤。 此外，该方法包括通过将转移基因组的表达量与转移组和/或非转移组中的转移预测基因的表达量进行比较来预测转移的可能性的步骤。 这里，转移预测基因组包括两个基因MSR1（NM_138716.1）和RET（M31213.1）。

公开(公告)号：US08129122B2

公开(公告)日：2012-03-06

申请号：US12310331

申请日：2007-03-30

申请人： Takashi Imai ,  Mayumi Iwakawa ,  Hitoshi Shibuya ,  Masahiko Miura ,  Ryoichi Yoshimura ,  Hiroshi Watanabe

发明人： Takashi Imai ,  Mayumi Iwakawa ,  Hitoshi Shibuya ,  Masahiko Miura ,  Ryoichi Yoshimura ,  Hiroshi Watanabe

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N15/11 ,  C12N15/12

CPC分类号： G01N33/57407 ,  C12Q1/6886 ,  C12Q2600/118 ,  G01N2333/705 ,  G01N2333/71

摘要： A method for objectively predicting possibility of metastasis to a cervical lymph node in an early stage for an individual case diagnosed as an oral cavity cancer, and a diagnosis kit to be used in the prediction are provided. The method includes a step of assaying expression amounts of metastasis prediction genes in which the expression amounts are changed between a metastasis group and a non-metastasis group, with respect to a sample collected from a primary legion of the oral cavity cancer. Further, the method includes a step of predicting the possibility of the metastasis by comparing the expression amounts of the metastasis gene group with the expression amounts of the metastasis prediction genes in a metastasis group and/or a non-metastasis group. Herein, the metastasis prediction gene group includes two genes MSR1 (NM_138716.1) and RET (M31213.1).

摘要翻译： 提供了用于客观预测诊断为口腔癌的个别病例的早期颈部淋巴结转移可能性的方法，以及用于预测的诊断试剂盒。 该方法包括相对于从口腔癌的主要团队收集的样品，测定转移组和非转移组之间的表达量发生变化的转移预测基因的表达量的步骤。 此外，该方法包括通过将转移基因组的表达量与转移组和/或非转移组中的转移预测基因的表达量进行比较来预测转移的可能性的步骤。 这里，转移预测基因组包括两个基因MSR1（NM_138716.1）和RET（M31213.1）。

公开(公告)号：US07678543B2

公开(公告)日：2010-03-16

申请号：US12214044

申请日：2008-06-16

申请人： Takashi Imai ,  Mayumi Iwakawa ,  Yuichi Michikawa

发明人： Takashi Imai ,  Mayumi Iwakawa ,  Yuichi Michikawa

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H2/04

CPC分类号： C12Q1/6846 ,  C12Q2565/518 ,  C12Q2531/119 ,  C12Q2527/119 ,  C12Q2527/101

摘要： A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.

摘要翻译： 提供了扩增基因组DNA的方法。 该方法包括以下步骤：（1）在9至12的pH和45至80℃的温度下培养含细胞的琼脂糖溶液以产生基因组DNA分散的琼脂糖溶液，其中0.002-1拷贝/ 5 单链基因组DNA的微升分散; （2）固定基因组DNA分散的琼脂糖溶液以产生基因组DNA分散的琼脂糖凝胶并中和凝胶的pH; 和（3）将具有链置换活性的DNA聚合酶，引物和dNTP加入到基因组DNA分散的琼脂糖凝胶中，并在0至60℃的温度下孵育该凝胶以扩增基因组DNA。

公开(公告)号：US20130214930A1

公开(公告)日：2013-08-22

申请号：US13881809

申请日：2011-10-27

申请人： Masanao Shiraishi ,  Takashi Imai ,  Takashi Kondo ,  Takayasu Ikeda ,  Yohei Iwata

发明人： Masanao Shiraishi ,  Takashi Imai ,  Takashi Kondo ,  Takayasu Ikeda ,  Yohei Iwata

IPC分类号： G08B5/00 ,  H05K5/00

CPC分类号： G08B5/00 ,  E05F15/43 ,  E05F15/74 ,  E05Y2900/132 ,  G01V8/20 ,  H05K5/0021

摘要： An automatic door sensor includes: at least one sensor module unit (sensor module 11) configured to detect optically an object or a human body and to output, according to the detection results, a signal to a door controller for controlling an automatic door to open/close; an accommodation unit (profile 12) capable of accommodating the at least one sensor module unit; a cover member (front cover 13) that is transmissive to light for use for the detection performed by the sensor module unit and configured to cover the accommodation unit in which the sensor module unit is accommodated; and a functionality expansion module (LED module 14) either side of which can be joined to the cover member and that is configured to expand functionalities of the automatic door sensor by performing communication with the sensor module unit.

摘要翻译： 自动门传感器包括：至少一个传感器模块单元（传感器模块11），被配置为光学地检测物体或人体，并根据检测结果向门控制器输出信号，以控制自动门打开 /关; 能够容纳至少一个传感器模块单元的容纳单元（轮廓12）; 覆盖构件（前盖13），其对于由传感器模块单元执行的用于检测的光可透射并且被配置为覆盖其中容纳传感器模块单元的容纳单元; 以及功能扩展模块（LED模块14），其两侧可以连接到盖构件，并且被配置为通过与传感器模块单元进行通信来扩展自动门传感器的功能。

公开(公告)号：US08362108B2

公开(公告)日：2013-01-29

申请号：US13080181

申请日：2011-04-05

申请人： Takashi Imai

发明人： Takashi Imai

IPC分类号： C09D11/10

CPC分类号： C09D11/324 ,  C08K5/06 ,  C08L101/06

摘要： An aqueous ink for a transfer ink jet recording method including applying to an intermediate transfer member a reaction liquid which forms an increased viscosity ink image by coming into contact with a coloring material in the ink, applying the ink to the intermediate transfer member by an ink jet device to form an intermediate image, and bringing a printing object into contact with the intermediate transfer member to transfer the image to the object. The aqueous ink contains a coloring material which is a polymer dispersion pigment dispersed in the aqueous medium with a polymer having a hydroxyl group in its structure as a dispersant, a nonionic surfactant which is an alcohol ether surfactant a 1% aqueous solution of which has a cloud point of 33° C. or less and which has a C4-C30 alkyl group and a propyleneoxy group in its structure, and an aqueous medium.

摘要翻译： 一种用于转印喷墨记录方法的水性油墨，其特征在于，向中间转印部件施加通过与油墨中的着色材料接触形成增加粘度的油墨图像的反应液体，通过油墨将油墨施加到中间转印部件 喷墨装置形成中间图像，并且使打印对象与中间转印部件接触以将图像转印到对象。 水性油墨含有作为分散在其结构中具有羟基的聚合物作为分散剂的水性介质中分散的聚合物分散颜料的着色材料，1％水溶液中的醇醚表面活性剂的非离子表面活性剂，其具有 浊点为33℃以下，其结构中具有C4-C30烷基和丙烯氧基，以及水性介质。

公开(公告)号：US08324293B2

公开(公告)日：2012-12-04

申请号：US11550464

申请日：2006-10-18

申请人： Takashi Imai ,  Shinichi Hakamada ,  Kenji Moribe ,  Masako Udagawa

发明人： Takashi Imai ,  Shinichi Hakamada ,  Kenji Moribe ,  Masako Udagawa

IPC分类号： C09D11/00

CPC分类号： C09D11/322 ,  C09D11/326

摘要： An aqueous ink comprising a polymer having hydroxyl groups and a pigment, which can provide images excellent in scratch resistance and highlighter resistance and can inhibit the seeping out phenomenon of the polymer and the deterioration of ejection characteristics even when it is stored for a long period of time. The aqueous ink comprises a polymer having hydroxyl groups, a pigment and a water-soluble organic solvent, wherein the water-soluble organic solvent comprises a poor medium for a polymer that is not adsorbed on the pigment (out of the polymer having hydroxyl groups), and wherein when the segment constituting the polymer that is not adsorbed on the pigment (out of the polymer having hydroxyl groups) is comprised of a segment A having hydroxyl groups and a segment B having no hydroxyl group, at least one of the poor medium is a good medium for either one of the segment A and the Segment B.

摘要翻译： 包含具有羟基的聚合物和颜料的水性油墨，其可以提供耐刮擦性和耐高温性优异的图像，并且可以抑制聚合物的渗出现象和喷射特性的劣化，即使当其长时间储存​​时 时间。 水性油墨包括具有羟基的聚合物，颜料和水溶性有机溶剂，其中水溶性有机溶剂包含不吸附在颜料上的聚合物的不良介质（在具有羟基的聚合物中） 并且其中当构成未吸附在颜料（在具有羟基的聚合物中）的聚合物的链段由具有羟基的链段A和不具有羟基的链段B组成时，至少一种不良培养基 是段A和段B之一的良好介质。