公开(公告)号：US06328895B1

公开(公告)日：2001-12-11

申请号：US09463688

申请日：2000-01-28

申请人： Takayuki Masuda ,  Kikuzo Kaneko ,  Kohei Sato ,  Fumihiko Matsuda

发明人： Takayuki Masuda ,  Kikuzo Kaneko ,  Kohei Sato ,  Fumihiko Matsuda

IPC分类号： B01Q1508

CPC分类号： B01D15/1828 ,  B01D2215/023

摘要： In separation of 3 components A, B and C having a C>B>A relation in respect of affinity for packing with a chromatographic separator comprising a circulation system formed by linking packing bed units 1 to 4 in endless series, the following Operations → → → → are performed in Step (1) as Stage 1, and the following Operations → → are repeatedly performed 3 times in subsequent Stages 2 to 4 in Step (2), provided that the fluid feed and withdrawal positions are respectively displaced one by one in the downstream direction of the circulation system every time after completion of each stage; : shutting off the circulation system, and withdrawing the whole of a component B fraction on the upstream side of the shutoff position while feeding a starting fluid material f to a packing bed unit on the downstream side of the shutoff position and feeding eluent D to the circulation system; : withdrawing part of a component A fraction while feeding eluent D to the circulation system; : withdrawing part of a component C fraction while feeding eluent D to the circulation system; and : circulating the internal fluid through the system with neither fluid feed to nor fluid withdrawal from the circulation system.

摘要翻译： 在分离具有C> B> A关系的3种组分A，B和C的关系中，包括通过连续填充床单元1至4连续形成的循环系统的色谱分离器的包装亲和力，以下操作<2 > - > <4> - > <1> - > <4> - > <3>在步骤（1）中作为阶段1执行，并且以下操作<2> - > <4> - > <3> 在步骤（2）中的后续阶段2至4中重复执行3次，条件是在每个阶段完成之后，流体进给和取出位置在循环系统的下游方向上分别逐个移位; 关闭循环系统，并且在关闭位置的上游侧取出部件B馏分的全部，同时将起始流体物料f供给到关闭位置的下游侧的填充床单元，并将洗脱液D供给循环 系统; <2>：将洗脱液D供给到循环中，抽出部分A组分 取出部分C部分，同时将洗脱液D送入循环系统; 和<4>：使内部流体通过系统循环，既没有流体供给也没有流体从循环系统中排出。

公开(公告)号：US06409922B1

公开(公告)日：2002-06-25

申请号：US09367522

申请日：1999-08-16

申请人： Kikuzo Kaneko ,  Takayuki Masuda ,  Fumihiko Matsuda ,  Kohei Sato ,  Kouji Tanikawa

发明人： Kikuzo Kaneko ,  Takayuki Masuda ,  Fumihiko Matsuda ,  Kohei Sato ,  Kouji Tanikawa

IPC分类号： B01D1508

CPC分类号： G01N30/466 ,  B01D15/1828 ,  B01D15/1842 ,  B01D15/361 ,  B01D53/02 ,  B01D53/025 ,  B01D2215/023 ,  C07H3/02 ,  C07H3/04 ,  C07H3/06 ,  C13B20/14 ,  C13B20/144 ,  C13B20/148 ,  C13B35/06 ,  C13K3/00 ,  C13K13/005 ,  G01N30/02 ,  B01D15/36

摘要： In chromatographic separation in a plurality of steps with a chromatographic separator packed with an ion exchanger used as at least part of chromatographic packing, at least part of the ionic form of at least part of the ion exchanger is changed to an ionic form fit for separation of components to be separated in each step to effect chromatographic separation, whereby a starting fluid material containing at least 3 components can be efficiently separated into at least 3 fractions. A preferred example of the chromatographic separator comprising a group of packing bed units linked in endless series to form a circulation flow path is provided with a shutoff valve at a position of the circulation flow path; starting fluid material feed means, desorbent feed means and 2-fraction withdrawal means for all the packing bed units; and a single-fraction withdrawal means connected to the circulation flow path on the upstream side of the shutoff valve and without any packing bed unit therebetween.

摘要翻译： 在使用填充有至少部分色谱填料的离子交换剂的色谱分离器的多个步骤中的色谱分离中，至少部分离子交换剂的至少部分离子形式被改变为适于分离的离子形式 在每个步骤中要分离的组分进行色谱分离，由此含有至少3种组分的起始流体材料可以有效地分离成至少3个级分。 包括以循环串联连接形成循环流路的一组填料床单元的色谱分离器的优选实例在循环流动路径的位置处设置有截止阀; 起始流体材料进料装置，解吸剂进料装置和用于所有填料床装置的2-馏分萃取装置; 以及连接到截流阀的上游侧的循环流路并且没有任何填料床单元的单馏分抽出装置。

公开(公告)号：US06331250B1

公开(公告)日：2001-12-18

申请号：US09308560

申请日：1999-05-20

申请人： Kikuzo Kaneko ,  Takayuki Masuda ,  Fumihiko Matsuda ,  Kohei Sato ,  Kouji Tanikawa

发明人： Kikuzo Kaneko ,  Takayuki Masuda ,  Fumihiko Matsuda ,  Kohei Sato ,  Kouji Tanikawa

IPC分类号： B01D1508

CPC分类号： B01D15/1828 ,  B01D15/361 ,  B01D2215/023

摘要： At least 2 different packings differing in the resolution of components contained in a starting fluid material such as a starting solution material and desired to be separated are used in a coexistent state to adjust the resolution of the components. The starting fluid material is flowed through the resultant packing bed to effect chromatographic separation, whereby high-purity fractions enriched with respective components can be obtained at high recoveries with a small amount of desorbent such as eluent. In a chromatographic separator, at least 2 packings comprising a first packing high in the resolution of a component having a medium affinity (affinity for packing) and a weak-affinity component and a second packing high in the resolution of a strong-affinity component and the medium-affinity component in a coexistent state are used as chromatographic packing in contact with a fluid containing the medium-affinity component to adjust the resolution of every two of at least 3 components, whereby at least 3 fractions can be obtained.

摘要翻译： 在起始溶液材料（例如起始溶液材料）中包含的期望分离的组分的分辨率不同的至少2种不同的填料以共存状态使用以调节组分的分辨率。 起始流体材料流过所得填料床以进行色谱分离，由此可以用少量解吸剂如洗脱液以高回收率获得富含各组分的高纯度馏分。 在色谱分离器中，包含至少2个包装，其具有高分辨率的具有中等亲和性（对包装的亲和性）和弱亲和性成分的成分的高度分离的第一包装，以及高亲和性成分分辨率的第二包装， 共存状态的中等亲和性成分用作与含有中等亲和力成分的流体接触的色谱填料，以调节至少3种成分中的每两种的分辨率，由此可以获得至少3个级分。

公开(公告)号：US6099654A

公开(公告)日：2000-08-08

申请号：US367690

申请日：1999-08-19

申请人： Kikuzo Kaneko ,  Takayuki Masuda ,  Fumihiko Matsuda ,  Kohei Sato ,  Kouji Tanikawa

发明人： Kikuzo Kaneko ,  Takayuki Masuda ,  Fumihiko Matsuda ,  Kohei Sato ,  Kouji Tanikawa

IPC分类号： B01D15/08 ,  C07C227/28 ,  C07C227/40 ,  C07C227/42 ,  C07C229/02 ,  C07C229/12 ,  G01N30/46 ,  G01N30/88 ,  C07B63/00

CPC分类号： C07C227/42 ,  C07C227/28

摘要： A starting solution material in the form of waste produced in the course of separation and recovery of sucrose from sugar beet extract or sugar beet molasses with a 2-component separation simulated moving bed chromatographic separator (using a strongly acidic cation exchange resin in a salt form as chromatographic packing) or its concentrate is used to separate therefrom a crude betaine fraction with a 2-component separation simulated moving bed chromatographic separator using as chromatographic packing a strongly acidic cation exchange resin in an ionic form equilibrated with that of the starting solution material. The crude betaine fraction or its concentrate is used to separate therefrom a betaine fraction with a 2-component separation simulated moving bed chromatographic separator using as chromatographic packing a strongly acidic cation exchange resin having at least 10% (based on the ion exchange capacity) of the ionic form thereof changed to the Ca form. The betaine fraction may be concentrated and subjected to crystallization, whereby high-purity betaine can be obtained.

摘要翻译： PCT No.PCT / JP98 / 05843 Sec。 371日期1999年8月19日 102（e）1999年8月19日PCT 1998年12月24日PCT PCT。 出版物WO99 /​​ 33784 日期1999年7月8日在使用2-组分分离模拟移动床色谱分离器（使用强酸性阳离子交换器）从甜菜提取物或甜菜糖蜜分离和回收蔗糖过程中产生的废物形式的起始溶液材料 树脂为盐形式作为色谱填充）或其浓缩物用于将粗制甜菜碱部分与2-组分分离模拟移动床层析分离器分离，使用与酸性阳离子交换树脂平衡的离子形式的强酸性阳离子交换树脂 起始溶液材料。 使用粗制甜菜碱级分或其浓缩物将其与2-组分分离模拟移动床层析分离器的甜菜碱级分分离，使用色谱填料将具有至少10％（基于离子交换容量）的强酸性阳离子交换树脂 其离子形式变为Ca形式。 可以将甜菜碱级分浓缩并进行结晶，由此可以获得高纯度的甜菜碱。

公开(公告)号：US5223143A

公开(公告)日：1993-06-29

申请号：US897795

申请日：1992-06-12

申请人： Takayuki Masuda ,  Tohru Sonobe ,  Kikuzo Kaneko ,  Fumihiko Matsuda

发明人： Takayuki Masuda ,  Tohru Sonobe ,  Kikuzo Kaneko ,  Fumihiko Matsuda

IPC分类号： B01D15/00 ,  B01D15/04 ,  B01D15/08 ,  B01D15/18 ,  C07H1/06 ,  G01N30/44 ,  G01N30/46 ,  G01N30/58 ,  G01N30/88

CPC分类号： C07H1/06 ,  B01D15/1828 ,  B01D2215/023 ,  G01N2030/445 ,  G01N2030/8813 ,  G01N30/461 ,  G01N30/468 ,  G01N30/58

摘要： There is provided a process for efficient chromatographic separation of a plurality of components from a fluid feed containing the same, which process comprises repeating a cycle comprising the step (1) of substantially shutting off the internal fluid circulation at a position of endless, or continuous, circulatory system and withdrawing a fraction enriched with a component having a strong affinity for an adsorbent upstream of the shut-off position while feeding the fluid feed downstream of the shut-off position, and the step (2) of withdrawing a plurality of fractions enriched with the remaining components while feeding a fluid desorbent and circulating the internal fluid.

摘要翻译： 提供了一种用于从含有该组分的流体进料高效色谱分离多个组分的方法，该方法包括重复一个循环，该循环包括步骤（1），其在无端或连续的位置基本上切断内部流体循环 循环系统，并且在向所述关闭位置的下游供给所述流体进料的同时，将在所述关闭位置上游的吸附剂的吸附剂富集的部分富集的部分富集，并且所述步骤（2）取出多个馏分 富含剩余的组分，同时供给流体解吸剂并循环内部流体。

公开(公告)号：US06224683B1

公开(公告)日：2001-05-01

申请号：US09402415

申请日：1999-10-04

申请人： Kouji Tanikawa ,  Fumihiko Matsuda ,  Kikuzo Kaneko ,  Makoto Tomizawa ,  Takayuki Masuda

发明人： Kouji Tanikawa ,  Fumihiko Matsuda ,  Kikuzo Kaneko ,  Makoto Tomizawa ,  Takayuki Masuda

IPC分类号： C07B6300

CPC分类号： C13B20/14 ,  B01D15/185 ,  B01D2215/023 ,  B01J39/26 ,  C13B20/144

摘要： In demineralization of a beet sugar solution before boiling as the starting solution with a simulated moving bed chromatographic separator comprising a plurality of packed column units packed with a strongly acidic cation exchange resin in a salt form having an average grain size of 300 to 500 &mgr;m and a uniformity coefficient of at most 1.2 as chromatographic packing (separating packing), and each provided with a starting solution feed inlet, an eluent water feed inlet, a withdrawal outlet for a fraction of a component having a strong affinity for chromatographic packing (“strong-affinity component”), and a withdrawal outlet for a fraction of a component having a weak affinity for chromatographic packing (“weak-affinity component”); use is made of circulating pumps, the number of which is at least half of the number of the packed column units, and the beet sugar solution before boiling is demineralized under the following conditions: starting solution feed rate/eluent water feed rate volume ratio=1/2.5 to 1/3.5, strong-affinity component fraction withdrawal rate/weak-affinity component fraction withdrawal rate volume ratio=0.5/1 to 1.2/1, and circulating flow rate=25 to 80 vol. % /hr. based on chromatographic packing in a slowest flow velocity zone in the circulation system, whereby a sucrose fraction having a high sucrose concentration is obtained using a small amount of eluent water with a large throughput.

摘要翻译： 在用沸腾前的甜菜糖溶液作为起始溶液进行脱矿化，其中模拟移动床层析分离器包含多个填充柱单元，其填充有平均粒径为300-500μm的盐形式的强酸性阳离子交换树脂，和 均匀系数最多为1.2，色谱填料（分离填料），每个均具有起始溶液进料口，洗脱水进料口，对色谱填料具有很强亲和力的成分部分的萃取出口（“强 - 亲和性组分“）和用于色谱填充弱亲和性的组分的一部分的提取出口（”弱亲和组分“）; 使用循环泵，其数量至少是填充柱单元数量的一半，煮沸前的甜菜糖溶液在以下条件下脱矿质：起始溶液进料速率/洗脱液进料速率体积比= 1 / 2.5〜1 / 3.5，强亲和成分馏分提取率/弱亲和成分馏分体积比= 0.5 / 1〜1.2 / 1，循环流量= 25〜80体积％。 ％/小时。 基于循环系统中最慢流速区域中的色谱填充，由此使用少量具有大生产量的洗脱水获得具有高蔗糖浓度的蔗糖级分。

公开(公告)号：US5534075A

公开(公告)日：1996-07-09

申请号：US308206

申请日：1994-09-19

申请人： Isamu Miyawaki ,  Kikuzo Kaneko

发明人： Isamu Miyawaki ,  Kikuzo Kaneko

IPC分类号： C12P19/14 ,  C12P19/02 ,  C13K1/08 ,  C13J1/06 ,  B01D15/08 ,  C02F1/00 ,  C02F1/28

CPC分类号： C13K1/08

摘要： Disclosed is a process for producing high-purity glucose by saccharifying liquefied starch with the aid of an enzyme, in which the saccharification reaction of the liquefied starch is discontinued at a point where the glucose content is less than 96% by weight, preferably within the range of 80 to 93% by weight, on a solid basis. The resulting saccharified solution is filtered, concentrated and softened as required, and then fractionated by subjecting it to column chromatography. Thereafter, the resulting glucose fraction is recovered. This process makes it possible to produce high-purity glucose efficiently in spite of a reduction in saccharification time.

摘要翻译： 公开了一种通过借助于酶对液化淀粉进行糖化来生产高纯度葡萄糖的方法，其中液化淀粉的糖化反应在葡萄糖含量小于96重量％的时间点停止，优选在 范围为80至93重量％，基于固体。 将所得糖化溶液过滤，浓缩并根据需要软化，然后通过使其进行柱色谱分离。 此后，回收得到的葡萄糖馏分。 该方法可以有效地生产高纯度葡萄糖，尽管糖化时间减少。