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    Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry
    3.
    发明授权
    Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry 失效
    使用质谱分析肽的C-末端氨基酸序列的方法

    公开(公告)号：US08119411B2

    公开(公告)日：2012-02-21

    申请号：US12973158

    申请日：2010-12-20

    申请人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Hiroaki Torii

    发明人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Hiroaki Torii

    IPC分类号： G01N33/00

    CPC分类号： G01N33/6821 ,  G01N33/6842 ,  G01N33/6851

    摘要： The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

    摘要翻译： 本发明提供了通过使用连续释放肽的C末端氨基酸的反应来分析肽的C末端氨基酸序列的方法，当连续释放C末端氨基酸的C末端氨基酸时，该方法可以抑制 长氨基酸长度的肽，这种不利的副反应，如在肽的中间位置处的肽键断裂，并且可以在广泛适用的条件下进行化学处理; 在该方法中，将具有长氨基酸长度的肽的干样品预先进行N-酰化处理; 通过使用其中链烷酸酐与少量全氟链烷酸组合的反应试剂，在温和条件下连续释放C-末端氨基酸; 应用水解处理; 然后通过胰蛋白酶消化进行精氨酸残基位点的选择性碎裂; 此后，使用MALDI-TOF-MS装置测量衍生自一系列反应产物的C-末端侧片段的分子量的降低; 鉴定出肽样品的C-末端氨基酸序列。

    METHOD FOR MODIFYING A PEPTIDE AND A METHOD FOR IDENTIFYING A PEPTIDE
    5.
    发明申请
    METHOD FOR MODIFYING A PEPTIDE AND A METHOD FOR IDENTIFYING A PEPTIDE 审中-公开
    用于修饰肽的方法和鉴定肽的方法

    公开(公告)号：US20110053196A1

    公开(公告)日：2011-03-03

    申请号：US12278711

    申请日：2007-02-08

    申请人： Kenji Miyazaki ,  Yo Tabuse ,  Hirotaka Minagawa ,  Emiko Saito ,  Ken'ichi Kamijo ,  Akira Tsugita ,  Kozue Tsugita

    发明人： Kenji Miyazaki ,  Yo Tabuse ,  Hirotaka Minagawa ,  Emiko Saito ,  Ken'ichi Kamijo ,  Akira Tsugita ,  Kozue Tsugita

    IPC分类号： C12Q1/37 ,  C07K1/113

    CPC分类号： G01N33/6803 ,  C07K1/107

    摘要： The present invention is to provide a method for easily and specifically modifying specific amino acid residue(s) constituting a peptide and to provide a methodology of improving the accuracy of identification of the peptide using a new information of the peptide obtained from the number of modified amino acid residue by said specific modification method as mentioned. The method for modifying a peptide according to the present invention is characterized:A method for modifying a peptide, wherein the peptide as supported in a substrate and an aqueous solution of perhalogenated carboxylic acid containing an alcohol is reacted to selectively esterify a glutamic acid residue of said peptide.The method for identifying a peptide according to the present invention is characterized:A method for identifying a peptide comprising the steps of: reacting the peptide as supported in a substrate and an aqueous solution of perhalogenated carboxylic acid containing an alcohol to selectively esterify glutamic acid residue of said peptide; immersing said substrate in a protease solution to obtain a peptide fragment originated from said peptide; measuring a molecular weight of said peptide fragment; and determining said peptide based on said molecular weight.

    摘要翻译： 本发明提供一种容易且特异性地修饰构成肽的特定氨基酸残基的方法，并且提供使用从修饰的数量获得的肽的新信息来提高肽的鉴定准确度的方法 通过所述具体的改性方法制备氨基酸残基。 本发明的肽的修饰方法的特征在于：对肽进行修饰的方法，其中负载在底物中的肽和含有醇的全卤代羧酸的水溶液进行反应以选择性地酯化谷氨酸残基 所述肽。 本发明鉴定肽的方法的特征在于：一种鉴定肽的方法，包括以下步骤：将载体载于底物中的肽与含有醇的全卤代羧酸水溶液反应以选择性酯化谷氨酸残基 的所述肽; 将所述底物浸入蛋白酶溶液中以获得源自所述肽的肽片段; 测量所述肽片段的分子量; 并基于所述分子量测定所述肽。

    Method Of Analyzing Protein
    6.
    发明申请
    Method Of Analyzing Protein 审中-公开
    分析蛋白质的方法

    公开(公告)号：US20090140136A1

    公开(公告)日：2009-06-04

    申请号：US11547516

    申请日：2005-02-08

    申请人： Hiroaki Torii ,  Kenji Miyazaki ,  Kenichi Kamijo ,  Akira Tsugita

    发明人： Hiroaki Torii ,  Kenji Miyazaki ,  Kenichi Kamijo ,  Akira Tsugita

    IPC分类号： B01D59/44

    CPC分类号： G01N33/6848 ,  G01N33/68

    摘要： The primary structure or the modification state of a protein is analyzed in detail. First, an analyte protein is subjected to PMF analysis (S101), and the gene of the protein is identified. Unidentified peaks not corresponding to the peaks of hypothetical peptide fragments are extracted, by comparing the hypothetical mass spectrum with the mass spectrum obtained by mass spectrometry of a sample protein (S102). Then, the unidentified peaks obtained are analyzed, and thus, the structure or properties of the protein, the presence and the kind of modification of amino acid residues, amino acid substitution, generation of mutants, and terminal cleavage are analyzed (S103).

    摘要翻译： 详细分析蛋白质的一级结构或修饰状态。 首先，对分析物蛋白进行PMF分析（S101），鉴定出蛋白质的基因。 通过将假想质谱与通过样品蛋白质谱质谱获得的质谱进行比较，提取不符合假想肽片段的峰的未鉴定的峰（S102）。 然后，分析所得到的未鉴定的峰，分析蛋白质的结构或性质，氨基酸残基的修饰，氨基酸取代，突变体的产生和末端切割的存在和种类（S103）。

    Block Copolymerized Polyimide Ink Composition for Printing
    8.
    发明申请
    Block Copolymerized Polyimide Ink Composition for Printing 审中-公开
    用于印刷的嵌段共聚聚酰亚胺油墨组合物

    公开(公告)号：US20080275181A1

    公开(公告)日：2008-11-06

    申请号：US11597694

    申请日：2005-05-24

    申请人： Maw Soe Win ,  Toshiyuki Goshima ,  Eika Kyo ,  Shintaro Nakajima ,  Noriki Hayashi ,  Tohru Kashiwagi ,  Kenji Miyazaki ,  Katsuya Yamada ,  Naoyuki Yamabayashi ,  Shoji Nakagama ,  Hiroshi Okuyama ,  Fumio Kasabo ,  Ippei Tanaka

    发明人： Maw Soe Win ,  Toshiyuki Goshima ,  Eika Kyo ,  Shintaro Nakajima ,  Noriki Hayashi ,  Tohru Kashiwagi ,  Kenji Miyazaki ,  Katsuya Yamada ,  Naoyuki Yamabayashi ,  Shoji Nakagama ,  Hiroshi Okuyama ,  Fumio Kasabo ,  Ippei Tanaka

    IPC分类号： C08G73/10

    CPC分类号： C09D11/102 ,  H05K3/28 ,  H05K2201/0154 ,  H05K2203/0783

    摘要： The object is to provide a polyimide ink composition having good printing properties and good continuous printing properties, which composition can be dried at a low temperature of not higher than 220° C., and which composition gives a coating film, after being dried, having excellent dimensional stability, heat resistance, low modulus of elasticity, flexibility, resistance to warping, chemical resistance, adhesiveness with substrates, and plating resistance. This object is accomplished by a polyimide ink composition for printing, comprising a mixed solvent containing an benzoic acid ester solvent and a glyme solvent, and a polyimide soluble in the mixed solvent; wherein the polyimide is obtained by polycondensing a polyimide oligomer with a tetracarboxylic dianhydride component(s) and/or a diamine component(s) having no siloxane bond in molecular skeleton thereof the polyimide oligomer being prepared by polycondensing a tetracarboxylic dianhydride component(s) and a diamine component(s) having siloxane bonds in molecular skeleton thereof in the presence of a base catalyst(s), or a mixed catalyst including a lactone(s) and/or an acidic compound(s) and a base(s); the content of the diamine component(s) having siloxane bonds based on the total diamine components being 15 to 85% by weight.

    摘要翻译： 本发明的目的是提供具有良好的印刷性能和良好的连续印刷性能的聚酰亚胺油墨组合物，该组合物可以在不高于220℃的低温下干燥，并且该组合物在干燥后得到涂膜， 优异的尺寸稳定性，耐热性，低弹性模量，柔软性，抗翘曲性，耐化学性，与基材的粘合性和耐电镀性。 该目的通过用于印刷的聚酰亚胺油墨组合物实现，其包含含有苯甲酸酯溶剂和甘醇二甲醚溶剂的混合溶剂和可溶于混合溶剂的聚酰亚胺; 其中所述聚酰亚胺通过聚酰亚胺低聚物与其分子骨架中不具有硅氧烷键的四羧酸二酐组分和/或二胺组分缩聚得到，所述聚酰亚胺低聚物是通过将四羧酸二酐组分和 在基础催化剂或含有内酯和/或酸性化合物和碱的混合催化剂存在下，在其分子骨架上具有硅氧烷键的二胺组分; 基于总二胺成分的具有硅氧烷键的二胺成分的含量为15〜85重量％。

    Method of analyzing c-terminal amino acid sequence of peptide
    9.
    发明申请
    Method of analyzing c-terminal amino acid sequence of peptide 失效
    分析肽末端氨基酸序列的方法

    公开(公告)号：US20060030052A1

    公开(公告)日：2006-02-09

    申请号：US10536824

    申请日：2003-11-28

    申请人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Takuji Nabetani

    发明人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Takuji Nabetani

    IPC分类号： G01N33/00

    CPC分类号： G01N33/6842 ,  G01N33/6821

    摘要： The present invention provides, as a method of analyzing the C-terminal amino acid sequence of a peptide with use of reaction technique for successively releasing the C-terminal amino acids, in which undesirable side reactions, such as cleavage of a peptide bond at the middle of the peptide, can be prevented and chemical treatments therein can be carried out under widely applicable conditions in the course of successive release of the C-terminal amino acids from a peptide, such a method comprising steps of dehydrating the gel on which a target peptide that has been separated by gel electrophoresis is held in the bound state; immersing it in a mixture solution of an alkanoic acid anhydride added with a small amount of a perfluoroalkanoic acid in a dipolar aprotic solvent to re-swell the gel carrier, forming a 5-oxazolone structure, at a temperature chosen in the range of from 30° C. to 80° C., followed by the cleavage of the 5-oxazolone ring to release the C-terminal amino acids, and then specifying the C-terminal amino acid sequence of the peptide based on the measured decrease in the molecular weight of a series of reaction products resulting therefrom.

    摘要翻译： 本发明提供了使用用于连续释放C-末端氨基酸的反应技术分析肽的C-末端氨基酸序列的方法，其中不期望的副反应，例如在 肽的中间，可以在广泛适用的条件下在肽的C末端氨基酸连续释放的过程中进行其中的化学处理，这种方法包括使凝胶脱水，其中将目标 通过凝胶电泳分离的肽保持在结合状态; 将其加入到加入少量全氟链烷酸的链烷酸与偶极非质子传递溶剂的混合溶液中，使溶胶载体重新溶胀，形成5-恶唑酮结构，温度范围为30℃ ℃至80℃，然后切割5-恶唑酮环以释放C末端氨基酸，然后根据测定的分子量减少指定肽的C-末端氨基酸序列 的一系列由此产生的反应产物。

    Expandable synthetic resin composition, synthetic resin foams, and methods for manufacturing synthetic resin foams
    10.
    发明授权
    Expandable synthetic resin composition, synthetic resin foams, and methods for manufacturing synthetic resin foams 失效
    可膨胀的合成树脂组合物，合成树脂泡沫以及制造合成树脂泡沫的方法

    公开(公告)号：US06300386B1

    公开(公告)日：2001-10-09

    申请号：US09269193

    申请日：1999-03-30

    申请人： Koichi Karukaya ,  Kenji Miyazaki ,  Koshi Kawabata

    发明人： Koichi Karukaya ,  Kenji Miyazaki ,  Koshi Kawabata

    IPC分类号： C08J910

    CPC分类号： C08J9/0061 ,  B29C44/50 ,  B29C44/505 ,  C08J9/0085 ,  C08J9/06 ,  C08J2201/03 ,  C08J2467/00

    摘要： An expandable synthetic resin composition capable of providing a synthetic resin foam excellent in both heat distortion resistance and mechanical strength, as well as such a synthetic resin foam, are provided. An expandable synthetic resin composition incorporating a thermoplastic resin, a liquid crystal resin and a heat-decomposable foaming agent, as well as a synthetic resin foam which incorporates a thermoplastic resin and a liquid crystal resin dispersed in the thermoplastic resin and exhibits an expansion ratio of 1.1-50, are provided.

    摘要翻译： 提供了能够提供耐热变形性和机械强度都优异的合成树脂发泡体的可膨胀合成树脂组合物，以及这种合成树脂发泡体。 一种包含热塑性树脂，液晶树脂和可热分解发泡剂的可膨胀合成树脂组合物，以及合成树脂泡沫体，其结合了热塑性树脂和分散在热塑性树脂中的液晶树脂，并显示膨胀比 提供1.1-50。