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    Reagent for biomaterials assay, preparation method thereof, and assay method
    1.
    发明授权
    Reagent for biomaterials assay, preparation method thereof, and assay method 失效
    生物材料测定试剂，其制备方法和测定方法

    公开(公告)号：US6010867A

    公开(公告)日：2000-01-04

    申请号：US867401

    申请日：1997-06-02

    申请人： Takeshi Kobayashi ,  Hiroyuki Honda ,  Ken-ichi Shimada

    发明人： Takeshi Kobayashi ,  Hiroyuki Honda ,  Ken-ichi Shimada

    IPC分类号： G01N33/533 ,  G01N33/543 ,  G01N33/58 ,  G01N33/53 ,  G01N33/552

    CPC分类号： G01N33/533 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/582 ,  Y10S436/80 ,  Y10S530/813

    摘要： The present invention relates to a reagent for biomaterials assay of (fluorescent dye).sub.n --avidin--a compound having a plurality of reactive groups--a biomaterial, or a reagent in which avidin is bound to a compound having a plurality of reactive groups through biotin in the above, a method for preparing it, a plastic fiber optics necessary for utilizing this, an assaying device having a sensing portion utilizing this fiber optics and an assaying method of biomaterials by use of this reagent and device. The assay method can be used for immunoassay of biomaterials such as antigens, enzymes contained in extremely minute amounts in blood or body fluid in medical diagnosis, and improves detectable sensitivity to a great extent.

    摘要翻译： 本发明涉及（荧光染料）正离子交换蛋白 - 具有多个反应性基团的化合物 - 生物材料或其中抗生物素蛋白通过生物素与具有多个反应性基团的化合物结合的试剂的生物材料测定试剂 在上述中，其制备方法，利用该方法所需的塑料光纤，具有利用该光纤的感测部分的测定装置和使用该试剂和装置的生物材料的测定方法。 该测定方法可用于免疫测定生物材料如抗原，在医学诊断中在血液或体液中极少量含有的酶，并在很大程度上改善可检测的敏感性。

    Plastic optical biomaterials assay device
    2.
    发明授权
    Plastic optical biomaterials assay device 失效
    塑料光学生物材料测定装置

    公开(公告)号：US5401469A

    公开(公告)日：1995-03-28

    申请号：US238782

    申请日：1994-05-06

    申请人： Takeshi Kobayashi ,  Hiroyuki Honda ,  Ken-ichi Shimada

    发明人： Takeshi Kobayashi ,  Hiroyuki Honda ,  Ken-ichi Shimada

    IPC分类号： G01N33/533 ,  G01N33/543 ,  G01N33/58 ,  G01N21/64

    CPC分类号： G01N33/54373 ,  G01N33/533 ,  G01N33/54353 ,  G01N33/582 ,  Y10S436/80 ,  Y10S436/805

    摘要： A reagent for biomaterials assay of (fluorescent dye).sub.n --avidin--a compound having a plurality of reactive groups--a biomaterial, or a reagent in which avidin is bound to a compound having a plurality of reactive groups through biotin in the above, a plastic fiber optics necessary for utilizing this, an assaying device having a sensing portion utilizing this fiber optics and an assaying method of biomaterials by use of this reagent and device. The assay method can be used for an immunoassay of biomaterials such as antigens, antibodies, and enzymes contained in extremely minute amounts in blood or body fluid for medical diagnosis, and improves the detectable sensitivity to a great extent.

    摘要翻译： （荧光染料）正离子交换蛋白 - 具有多个反应性基团的化合物 - 生物材料或其中抗生物素蛋白通过上述生物素与具有多个反应性基团的化合物结合的试剂的生物材料测定试剂， 使用该材料所需的塑料光纤，具有利用该光纤的感测部分的测定装置和通过使用该试剂和装置的生物材料的测定方法。 测定方法可用于生物材料的免疫测定，例如用于医学诊断的血液或体液中极少量含有的抗原，抗体和酶，并且在很大程度上提高了可检测的敏感性。

    Method for producing optical fiber having formyl groups on core surface thereof
    3.
    发明授权
    Method for producing optical fiber having formyl groups on core surface thereof 失效
    在其芯表面上具有甲酰基的光纤的制造方法

    公开(公告)号：US5354574A

    公开(公告)日：1994-10-11

    申请号：US80615

    申请日：1993-06-22

    申请人： Takeshi Kobayashi ,  Shinji Iijima ,  Ken-ichi Shimada ,  Kazue Ohe ,  Yasunori Sakai

    发明人： Takeshi Kobayashi ,  Shinji Iijima ,  Ken-ichi Shimada ,  Kazue Ohe ,  Yasunori Sakai

    IPC分类号： G01N33/545 ,  G01N33/547 ,  B05D5/06

    CPC分类号： G01N33/547 ,  G01N33/545

    摘要： The present invention is directed to a method for producing an optical fiber having formyl groups on a core surface. In one embodiment, a plastic optical fiber having formyl groups on a core surface is obtained by immersing the core surface of the plastic optical fiber in a treating solution containing at least a 0.5 to 40 mM alkali metal hydroxide and a compound having formyl groups in water or lower alcohol solvent. In the other embodiment, a plastic optical fiber having formyl groups on a core surface is obtained by introducing a vic-diol group, a primary hydroxyl group or an alkenyl group on the core surface, and then oxidizing it. Turbidity on the core surface of the optical fiber thus obtained is minimized, thereby reducing the transmittance losses of light.

    摘要翻译： 本发明涉及一种在芯表面上制备具有甲酰基的光纤的方法。 在一个实施方案中，通过将塑料光纤的芯表面浸渍在含有至少0.5至40mM碱金属氢氧化物和在水中具有甲酰基的化合物的处理溶液中来获得在芯表面上具有甲酰基的塑料光纤 或低级醇溶剂。 在另一实施方式中，通过在芯表面上引入邻二醇基，伯羟基或烯基，然后将其氧化来获得在芯表面上具有甲酰基的塑料光纤。 由此获得的光纤的芯表面上的浊度最小化，从而降低光的透射率损失。

    Optical fiber based fluorescent immunoassay apparatus
    4.
    发明授权
    Optical fiber based fluorescent immunoassay apparatus 失效
    基于光纤的荧光免疫测定仪

    公开(公告)号：US5449625A

    公开(公告)日：1995-09-12

    申请号：US98394

    申请日：1993-08-16

    申请人： Takeshi Kobayashi ,  Shinji Iijima ,  Yasunori Sakai ,  Ken-ichi Shimada

    发明人： Takeshi Kobayashi ,  Shinji Iijima ,  Yasunori Sakai ,  Ken-ichi Shimada

    IPC分类号： G01N21/64 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58

    CPC分类号： G01N33/54366 ,  G01N33/5302 ,  G01N33/582 ,  Y10S435/968 ,  Y10S435/969 ,  Y10S436/80 ,  Y10S436/807

    摘要： The present invention relates to an apparatus for performing a fluoroimmunoassay, the apparatus comprising an excitation light source, a fluorescence detector, an optical fiber for an excitation light from the excitation light source to which an antigen or antibody is bound, and a spectroscope, in which the mentioned antigen or antibody is bound to an outgoing end surface of the optical fiber for the excitation light. In the apparatus the spectroscope is constructed so that it introduces the excitation light emitted from the excitation light source to a incident end surface of the optical fiber for the excitation light and introduces a fluorescence emitted from a fluorescence-labeled antibody or antigen which forms an immunological complex with the antigen or antibody to the fluorescence detector. In the apparatus the outgoing end surface of the optical fiber for the excitation light has a transmittance for the excitation light from the excitation light source. The invention also relates to a sensing chip which is used in the apparatus for performing a fluoroimmunoassay therewith.

    摘要翻译： PCT No.PCT / JP92 / 01652 Sec。 371日期：1993年8月16日 102（e）日期1993年8月16日PCT提交1992年12月17日PCT公布。 公开号WO93 / 13418 日期：1993年7月8日。本发明涉及一种用于进行荧光免疫测定的装置，该装置包括激发光源，荧光检测器，用于来自激发光源的激发光的光纤，抗原或抗体 所述抗原或抗体结合到所述光纤的出射端表面用于激发光。 在该装置中，分光镜被构造成使得从激发光源发射的激发光引入用于激发光的光纤的入射端面，并引入从形成免疫学的荧光标记的抗体或抗原发射的荧光 与荧光检测器的抗原或抗体复合。 在该装置中，用于激发光的光纤的出射端面对于来自激发光源的激发光具有透射率。 本发明还涉及用于与其进行荧光免疫测定的装置中的感测芯片。

    Cell culture method and cell sheet
    7.
    发明申请
    Cell culture method and cell sheet 审中-公开
    细胞培养方法和细胞片

    公开(公告)号：US20060063252A1

    公开(公告)日：2006-03-23

    申请号：US11226247

    申请日：2005-09-15

    申请人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata ,  Hiroko Terasaki

    发明人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata ,  Hiroko Terasaki

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0629 ,  C12M25/00 ,  C12N5/0075 ,  C12N11/14 ,  C12N13/00

    摘要： Epidermal keratinocytes not incorporating magnetic particles therein and epidermal keratinocytes incorporating magnetic particles therein were seeded in a 24-well ultra-low-attachment plate, and cultured for one day after a neodymium magnet was placed outside the bottom of the well. As a result, control epidermal keratinocytes not incorporating magnetic particles therein did not adhere to the bottom of the well and did not form an undifferentiated cell sheet. On the contrary, epidermal keratinocytes incorporating magnetic particles therein, while being attracted to the bottom of the well by magnetic force, formed a multilayered cell sheet. This cell sheet could be easily recovered by removing the neodymium magnet placed outside the bottom of the well, then bringing a magnet having a hydrophilic film attached thereto close to the cell sheet from upside and to attract it via this hydrophilic film, and lifting it.

    摘要翻译： 将不含有磁性颗粒的表皮角化细胞和其中含有磁性颗粒的表皮角质形成细胞接种在24孔超低附着板中，并在将钕磁铁放置在孔底部之外培养1天。 结果，不含有磁性颗粒的对照表皮角化细胞不粘附到孔的底部，并且不形成未分化的细胞片。 相反，在其中通过磁力吸引到井底部的同时包含磁性颗粒的表皮角质形成细胞形成多层细胞片。 通过去除放置在孔底部的钕磁铁，可以容易地回收该细胞片，然后将从其上方附着有亲水膜的磁体附着到细胞片上，并通过该亲水性膜吸引，并将其提起。

    Cell culture method and cultured tissue
    8.
    发明申请
    Cell culture method and cultured tissue 审中-公开
    细胞培养法和培养组织

    公开(公告)号：US20060121606A1

    公开(公告)日：2006-06-08

    申请号：US11271911

    申请日：2005-11-14

    申请人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata

    发明人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0062

    摘要： A cell culture method prepares first cells that are adhesion-dependent cells and monolayered or multilayered cells on a culture surface of a culture substrate, seeds second cells that are adhesion-dependent cells and are magnetized by allowing to have magnetic particles on the first cells, induces the second cells to a predetermined position on the first cells by magnetic force, and cultures the first cells and the second cells in a cell arrangement obtained by the magnetic induction. According to this cell culture method, after a cell sheet was prepared individually, cells can be multilayered without changing a temperature, peeling the monolayered sheet and laminating the monolayered sheets.

    摘要翻译： 细胞培养方法在培养基底的培养表面上制备作为粘附依赖性细胞和单层或多层细胞的第一细胞，作为粘附依赖性细胞的种子第二细胞并通过在第一细胞上具有磁性颗粒而被磁化， 通过磁力将第二细胞诱导到第一细胞上的预定位置，并以通过磁感应获得的细胞排列来培养第一细胞和第二细胞。 根据该细胞培养方法，单独制备细胞片后，细胞可以多层而不改变温度，剥离单层片材并层压单层片材。