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1.发明授权Hepatocyte growth factor activating protease and gene encoding the protease 失效肝细胞生长因子激活蛋白酶和编码蛋白酶的基因
公开(公告)号：US5677164A
公开(公告)日：1997-10-14
申请号：US448937
申请日：1995-05-24
IPC分类号： C07K14/475 , C12N9/64 , C12N15/12 , C12N15/57
CPC分类号： C12N9/64 , C07K14/475 , C07K14/4753 , C12N9/6421
摘要： A gene encoding a protease derived from human serum and having an activity to convert single-chain hepatocyte growth factor (HGF) into active two-chain HGF and a method of producing the protease by the use of said gene are provided. A method of producing a precursor protein of said protease is also provided.
摘要翻译： 提供了编码来自人血清并具有将单链肝细胞生长因子（HGF）转化为活性双链HGF的活性的基因的编码基因和使用所述基因产生蛋白酶的方法。 还提供了产生所述蛋白酶的前体蛋白质的方法。
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2.发明授权Hepatocyte growth factor activating protease and gene encoding the protease 失效肝细胞生长因子激活蛋白酶和编码蛋白酶的基因
公开(公告)号：US5466593A
公开(公告)日：1995-11-14
申请号：US148910
申请日：1993-11-05
CPC分类号： C12N9/64 , C07K14/475 , C07K14/4753 , C12N9/6421
摘要： A gene encoding a protease derived from human serum and having an activity to convert single-chain hepatocyte growth factor (HGF) into active two-chain HGF and a method of producing the protease by the use of said gene are provided. A method of producing a precursor protein of said protease is also provided.
摘要翻译： 提供了编码来自人血清并具有将单链肝细胞生长因子（HGF）转化为活性双链HGF的活性的基因的编码基因和使用所述基因产生蛋白酶的方法。 还提供了产生所述蛋白酶的前体蛋白质的方法。
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3.发明授权Protein having nitrile hydratase activity obtained from rhizobium, gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene 失效从根瘤菌获得的腈水合酶活性的蛋白质，编码基因的基因，以及通过含有该基因的转化体从腈生产酰胺的方法
公开(公告)号：US5314819A
公开(公告)日：1994-05-24
申请号：US52681
申请日：1993-04-27
摘要： A novel water-soluble enzyme protein being composed of three heterogeneous subunits and having nitrile hydratase activity was separated and purified from the bacterium of genus Rhizobium. The nucleotide sequence of the gene encoding the protein was determined; using the transformant transformed with the expression vector having the nucleotide sequence, the protein was expressed; by reacting the transformant with a nitrile in a culture broth, the corresponding amide was produced.Via the use of a culture broth containing the transformant described above or the protein itself, a nitrile can be efficiently converted into the corresponding amide.
摘要翻译： 由三种异质亚基组成并具有腈水合酶活性的新型水溶性酶蛋白从根瘤菌属细菌中分离纯化。 测定编码该蛋白质的基因的核苷酸序列; 使用用具有核苷酸序列的表达载体转化的转化体，表达蛋白质; 通过使转化体与培养液中的腈反应，生成相应的酰胺。 通过使用含有上述转化体的培养液或蛋白质本身，腈可以有效地转化为相应的酰胺。
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4.发明授权
公开(公告)号：US5436145A
公开(公告)日：1995-07-25
申请号：US246570
申请日：1994-05-20
CPC分类号： C12P17/12 , Y10S435/822 , Y10S435/824 , Y10S435/84 , Y10S435/843 , Y10S435/847 , Y10S435/85 , Y10S435/859 , Y10S435/874 , Y10S435/876 , Y10S435/877 , Y10S435/88 , Y10S435/881
摘要： 6-Hydroxy nitrogen-containing 6-membered ring compounds of the following general formula (II): ##STR1## wherein R.sup.1 represents carboxy group, carbamoyl group, cyano group, formyl group, C.sub.1 -C.sub.5 hydroxyalkyl group, C.sub.2 -C.sub.6 alkoxycarbonyl group, carboxyvinyl group, carboxymethyl group or oxime group, R.sup.2 represents hydrogen atom or carboxy group, and A represents carbon atom or nitrogen atom, can be prepared by reacting a nitrogen-containing 6-membered ring compounds of the following general formula (I): ##STR2## wherein R.sup.1, R.sup.2 and A are as defined in the general formula (II) above, with a microorganism or physico-chemically treated microorganism in an aqueous medium. Efficiency of the above reaction can be raised by conducting the reaction in the presence of phenazine methosulfate.
摘要翻译： 下述通式（II）的6-羟基含氮6元环化合物：其中R 1表示羧基，氨基甲酰基，氰基，甲酰基，C 1 -C 5羟基烷基，C 2 -C 6 烷氧基羰基，羧基乙烯基，羧甲基或肟基，R2表示氢原子或羧基，A表示碳原子或氮原子，可以通过使下述通式（I）的含氮6元环化合物 ）：其中R 1，R 2和A如上述通式（II）中所定义，其在水性介质中与微生物或物理化学处理的微生物一起使用。 可以通过在吩嗪甲硫酸盐存在下进行反应来提高上述反应的效率。
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5.发明授权Process for the production of human apolipoprotein E, and transformed hosts and products thereof 失效生产人APOLIPOPROTEIN E的过程及其变形的主体及产品
公开(公告)号：US5116739A
公开(公告)日：1992-05-26
申请号：US288470
申请日：1988-12-22
申请人： Yutaka Teranishi , Nobuhiko Takamatsu , Yasushi Matsui , Masako Kimura , Yasuko Ikeda , Yuuki Morimoto
发明人： Yutaka Teranishi , Nobuhiko Takamatsu , Yasushi Matsui , Masako Kimura , Yasuko Ikeda , Yuuki Morimoto
IPC分类号： C07K14/775 , C12N15/12 , C12N15/70 , C12N15/81 , C12N15/85
CPC分类号： C07K14/775 , C12N15/70 , C12N15/81 , C12N15/85
摘要： The invention provides DNA fragments coding for human apolipoprotein E or human apolipoprotein E-like substances having physiological activities equivalent to those of said human apolipoprotein E, expression vectors suitable for production of such proteins, hosts for use in the production, and process for the production, as well as such proteins thereby produced.
摘要翻译： 本发明提供编码人载脂蛋白E或具有与所述人载脂蛋白E相当的生理活性的人载脂蛋白E或人载脂蛋白E样物质的DNA片段，适用于生产这种蛋白质的表达载体，用于生产的宿主和用于生产的方法 ，以及由此产生的这样的蛋白质。
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