公开(公告)号：US20170296583A1

公开(公告)日：2017-10-19

申请号：US15515996

申请日：2015-09-30

申请人： Antnor Limited

发明人： Anthony Galea ,  Irina Brokhman

IPC分类号： A61K35/14 ,  A61K38/20 ,  A61K38/18 ,  C12N9/64 ,  C07K1/14

CPC分类号： A61K35/14 ,  A61K38/1841 ,  A61K38/1858 ,  A61K38/1866 ,  A61K38/2006 ,  C07K1/14 ,  C12N9/64 ,  A61K2300/00

摘要： Described are methods of producing an autologous composition useful for treatment of damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal. The method comprises preparing an anti-inflammatory/anti-catabolic component of the autologous composition comprising IL-1ra and TIMPs. An anti-inflammatory/anti-catabolic component is prepared comprising: collecting blood from the mammal; delivering the blood to a tube; incubating the blood at a temperature of from about 37° C. to about 39° C. for about 24 hours, preferably in the presence of sodium citrate; centrifuging the blood to separate the blood into a supernatant component and a cellular fraction; and collecting the supernatant component. The method further comprises preparing a regenerative component of the autologous composition comprising: collecting blood from the mammal; delivering the blood to a tube in the presence of about 4% citric acid; centrifuging the blood to separate a platelet-rich plasma component from a whole blood; collecting the platelet-rich plasma component; and mixing the supernatant component with the platelet-rich plasma component to provide the autologous composition. Also provided is a method of treating damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a subject with the autologous composition, an autologous composition for treating damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal and the use of the autologous composition for the treatment of damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal.

公开(公告)号：US20140099297A1

公开(公告)日：2014-04-10

申请号：US14026887

申请日：2013-09-13

申请人： Apeiron Biologics AG

发明人： Manfred Schuster ,  Hans Loibner ,  Evelyne Janzek-Hawlat ,  Bernhard Peball ,  Stefan Stranner ,  Bettina Wagner ,  Robert Weik

IPC分类号： A61K38/48

CPC分类号： A61K38/4813 ,  A61K38/00 ,  C07K14/70596 ,  C12N9/48 ,  C12N9/64

摘要： The present invention relates to recombinant ACE2 polypeptide, where the ACE2 polypeptide is present as a dimer. The dimer is formed specifically from glycosylated monomers and is used for producing pharmaceutical products with an extended half-life

摘要翻译： 本发明涉及重组ACE2多肽，其中ACE2多肽作为二聚体存在。 二聚体特异性地由糖基化单体形成，并用于生产延长半衰期的药物产品

公开(公告)号：US20090297436A1

公开(公告)日：2009-12-03

申请号：US12366567

申请日：2009-02-05

申请人： Leon Garcia-Martinez ,  Anne Elisabeth Carvalho Jensen ,  Katie Olsen ,  Ben Dutzar ,  Ethan Ojala ,  Brian Kovacevich ,  John Latham ,  Jeffrey T. L. Smith

发明人： Leon Garcia-Martinez ,  Anne Elisabeth Carvalho Jensen ,  Katie Olsen ,  Ben Dutzar ,  Ethan Ojala ,  Brian Kovacevich ,  John Latham ,  Jeffrey T. L. Smith

IPC分类号： A61K51/00 ,  C07K16/24 ,  C07H21/04 ,  C12N15/63 ,  C12N1/19 ,  A61K39/395 ,  A61P35/00 ,  A61K49/00 ,  C07K19/00 ,  C12Q1/68

CPC分类号： C07K16/248 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  C12N9/60 ,  C12N9/64 ,  C12N15/81 ,  C12P21/02

摘要： The present invention is directed to antibodies and fragments thereof and humanized versions thereof having binding specificity for IL-6. Another embodiment of this invention relates to the antibodies described herein, and binding fragments thereof, comprising the sequences of the VH, VL and CDR polypeptides described herein, and the polynucleotides encoding them. The invention also contemplates conjugates of anti-IL-6 antibodies and binding fragments thereof conjugated to one or more functional or detectable moieties. The invention also contemplates methods of making said anti-IL-6 antibodies and binding fragments thereof. Embodiments of the invention also pertain to the use of anti-IL-6 antibodies, and binding fragments thereof, for the diagnosis, assessment and treatment of diseases and disorders associated with IL-6. These antibodies may bind at least one of soluble IL-6, cell surface expressed IL-6, IL-6/IL-6R and/or prevent the association of IL-6 and IL-6R, the association of IL-6/IL-6R and gpl30 and or the formation of IL-6/IL-6R/gpl30 multimers and thereby inhibit a biological effect associated with any of the foregoing.

摘要翻译： 本发明涉及对IL-6具有结合特异性的抗体及其片段和人源化形式。 本发明的另一个实施方案涉及本文所述的抗体及其结合片段，其包含本文所述的VH，VL和CDR多肽的序列，以及编码它们的多核苷酸。 本发明还涉及抗IL-6抗体及其与一个或多个功能或可检测部分缀合的结合片段的缀合物。 本发明还考虑制备所述抗IL-6抗体及其结合片段的方法。 本发明的实施方案还涉及抗IL-6抗体及其结合片段用于诊断，评估和治疗与IL-6相关的疾病和病症的用途。 这些抗体可以结合可溶性IL-6，细胞表面表达的IL-6，IL-6 / IL-6R中的至少一种和/或阻止IL-6和IL-6R的结合，IL-6 / IL -6R和gp130和/或IL-6 / IL-6R / gp130多聚体的形成，从而抑制与前述任何相关的生物学效应。

公开(公告)号：US20080182777A1

公开(公告)日：2008-07-31

申请号：US11807589

申请日：2007-05-29

申请人： Chandra J. Panchal ,  Jinzi Jason Wu ,  Richard Beliveau ,  Marcia Ruiz ,  Seema Garde ,  Borhane Annabi ,  Sylvie Lamy ,  Mounia Bouzeghrane ,  Luc Daigneault ,  Robert Hawkins

发明人： Chandra J. Panchal ,  Jinzi Jason Wu ,  Richard Beliveau ,  Marcia Ruiz ,  Seema Garde ,  Borhane Annabi ,  Sylvie Lamy ,  Mounia Bouzeghrane ,  Luc Daigneault ,  Robert Hawkins

IPC分类号： A61K38/00

CPC分类号： A61K38/1709 ,  A61K38/10 ,  A61K38/17 ,  A61K38/46 ,  A61K38/52 ,  A61K48/00 ,  C07H21/04 ,  C07K14/47 ,  C12N9/64 ,  C12N9/6491 ,  C12Q1/68

摘要： Matrix metalloproteinases (MMPs) play an important role in morphogenesis, angiogenesis, wound healing, and in certain disorders such as rheumatoid arthritis, tumor invasion and metastasis. MMPs are thought to be regulated by a variety of cytokines, growth factors, hormones and phorbol esters. This regulation occurs on three levels; alteration of gene expression, activation of the latent zymogen and inhibition by the tissue inhibitors of metalloproteinases (TIMP). We report here a new agent that regulates the level of MMPs.

摘要翻译： 基质金属蛋白酶（MMP）在形态发生，血管生成，伤口愈合以及某些疾病如类风湿性关节炎，肿瘤浸润和转移中起重要作用。 认为MMP被多种细胞因子，生长因子，激素和佛波酯所调节。 这种规定发生在三个层次上 基因表达的改变，潜在酶原的活化和金属蛋白酶（TIMP）的组织抑制剂的抑制。 我们在这里报告一种调节MMPs水平的新药物。

公开(公告)号：US20060269538A1

公开(公告)日：2006-11-30

申请号：US11441635

申请日：2006-05-26

申请人： Andre Koltermann ,  Ulrich Kettling ,  Ulrich Haupts ,  Wayne Coco ,  Jan Tebbe ,  Christian Votsmeier ,  Andreas Scheidig

发明人： Andre Koltermann ,  Ulrich Kettling ,  Ulrich Haupts ,  Wayne Coco ,  Jan Tebbe ,  Christian Votsmeier ,  Andreas Scheidig

IPC分类号： A61K38/48 ,  C07H21/04 ,  C12P21/06 ,  C12N9/64 ,  C40B40/10 ,  C40B50/06

CPC分类号： C12Q1/37 ,  A61K38/00 ,  C12N9/64 ,  G01N2500/00

摘要： The present invention provides variants of serine proteases of the S1 class with altered sensitivity to one or more activity-modulating substances. A method for the generation of such proteases is disclosed, comprising the provision of a protease library encoding polynucleotide sequences, expression of the enzymes, screening of the library in the presence of one or several activity-modulating substances, selection of variants with altered sensitivity to one or several activity-modulating substances and isolation of those polynucleotide sequences that encode for the selected variants.

摘要翻译： 本发明提供对一种或多种活性调节物质敏感性改变的S1类丝氨酸蛋白酶的变体。 公开了产生这种蛋白酶的方法，包括提供编码多核苷酸序列的蛋白酶文库，酶的表达，在一种或几种活性调节物质存在下筛选文库，选择对 一种或几种活性调节物质以及编码所选变体的那些多核苷酸序列的分离。

公开(公告)号：US20040101927A9

公开(公告)日：2004-05-27

申请号：US09764856

申请日：2001-01-17

发明人： Craig A. Rosen ,  Steven M. Ruben ,  Steven C. Barash

IPC分类号： C12P021/02 ,  C07H021/04 ,  C12N005/06

CPC分类号： A61K31/00 ,  A61K38/17 ,  A61K48/00 ,  C07H21/04 ,  C07K14/435 ,  C07K14/47 ,  C07K14/575 ,  C12N5/06 ,  C12N9/00 ,  C12N9/64 ,  C12P21/02 ,  C12Q1/68

摘要： The present invention relates to novel proteins. More specifically, isolated nucleic acid molecules are provided encoding novel polypeptides. Novel polypeptides and antibodies that bind to these polypeptides are provided. Also provided are vectors, host cells, and recombinant and synthetic methods for producing human polynucleotides and/or polypeptides, and antibodies. The invention further relates to diagnostic and therapeutic methods useful for diagnosing, treating, preventing and/or prognosing disorders related to these novel polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of polynucleotides and polypeptides of the invention. The present invention further relates to methods and/or compositions for inhibiting or enhancing the production and function of the polypeptides of the present invention.

公开(公告)号：US06638507B1

公开(公告)日：2003-10-28

申请号：US09650284

申请日：2000-08-29

申请人： Sriram Balasubramanian ,  John Ford ,  Daniel M. Gorman ,  Gerard Zurawski

发明人： Sriram Balasubramanian ,  John Ford ,  Daniel M. Gorman ,  Gerard Zurawski

IPC分类号： A61K39395

CPC分类号： C07K16/40 ,  C12N9/64 ,  C12N9/6421 ,  G01N2333/96425 ,  Y10S435/81

摘要： Nucleic acids encoding various proteases, from a mammal, reagents related thereto, including specific antibodies, and purified proteins are described. Methods of using said reagents and related diagnostic kits are also provided.

摘要翻译： 描述了编码来自哺乳动物的各种蛋白酶，与其相关的试剂（包括特异性抗体）和纯化的蛋白质的核酸。 还提供了使用所述试剂和相关诊断试剂盒的方法。

公开(公告)号：US20030005474A1

公开(公告)日：2003-01-02

申请号：US10109548

申请日：2002-03-28

发明人： Michael W. Leviten

IPC分类号： A01K067/027 ,  C12N005/06 ,  C12N015/85

CPC分类号： A01K67/0276 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0306 ,  A01K2267/0356 ,  A01K2267/0393 ,  A61K38/00 ,  C12N9/64 ,  C12N15/8509 ,  C12N2800/30

摘要： The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in an ADAM-like protease gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression and gene function, and as potential treatments for various disease states and disease conditions.

摘要翻译： 本发明涉及转基因动物，以及与基因功能表征相关的组合物和方法。 具体地说，本发明提供了包含ADAM样蛋白酶基因突变的转基因小鼠。 这样的转基因小鼠可用作疾病的模型并用于鉴定调节基因表达和基因功能的试剂，以及作为各种疾病状态和疾病状况的潜在治疗。

公开(公告)号：US20020081704A1

公开(公告)日：2002-06-27

申请号：US09741150

申请日：2000-12-21

发明人： Karl Guegler ,  Marion Webster ,  Chunhua Yan ,  Wei Shao ,  Karen A. Ketchum ,  Valentina Di Francesco ,  Ellen M. Beasley

IPC分类号： C12N009/64 ,  G01N033/53 ,  C12Q001/68 ,  C12P021/02 ,  C12N005/06

CPC分类号： C12N9/64 ,  G01N33/573 ,  G01N2333/96433 ,  G01N2500/04 ,  Y10T436/143333

摘要： The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the protease peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the protease peptides, and methods of identifying modulators of the protease peptides.

摘要翻译： 本发明提供由人基因组内的基因编码的肽的氨基酸序列，本发明的蛋白酶肽。 本发明特别提供了分离的肽和核酸分子，鉴定蛋白酶多肽的直向同源物和旁系同源物的方法，以及鉴定蛋白酶肽调节剂的方法。

公开(公告)号：US4751084A

公开(公告)日：1988-06-14

申请号：US929950

申请日：1986-11-12

申请人： Joseph Feder ,  William R. Tolbert ,  Thomas W. Rademacher ,  Raj B. Parekh ,  Raymond A. Dwek

发明人： Joseph Feder ,  William R. Tolbert ,  Thomas W. Rademacher ,  Raj B. Parekh ,  Raymond A. Dwek

IPC分类号： C12N5/00 ,  A61K38/00 ,  C08B37/00 ,  C12N9/64 ,  C12N9/48 ,  A61K37/547 ,  C12N9/50

CPC分类号： C08B37/00 ,  C12N9/64 ,  A61K38/00 ,  Y10S514/822

摘要： Glycosylated plasminogen activator (t-PA) having a glycosylation pattern significantly different than exhibited by t-PA from Bowes melanoma cells is obtained from cultured normal human colon fibroblast cells (CCD-18Co).

摘要翻译： 从培养的正常人结肠成纤维细胞（CCD-18Co）获得具有与Bowes黑素瘤细胞的t-PA显示不同的糖基化模式的糖基化纤溶酶原激活剂（t-PA）。