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9 条记录 (耗时 0.031 秒)

    Mutant Smg p21 protein with GTP binding activity
    3.
    发明授权
    Mutant Smg p21 protein with GTP binding activity 失效
    具有GTP结合活性的突变Smg p21蛋白

    公开(公告)号：US5378810A

    公开(公告)日：1995-01-03

    申请号：US949105

    申请日：1992-09-21

    申请人： Yoshimi Takai ,  Jun Kondo ,  Yasushi Matsui ,  Yutaka Teranishi ,  Rie Matsui

    发明人： Yoshimi Takai ,  Jun Kondo ,  Yasushi Matsui ,  Yutaka Teranishi ,  Rie Matsui

    IPC分类号： C07K14/47 ,  C07K14/705 ,  C07K13/00 ,  C12N15/00

    CPC分类号： C07K14/705 ,  C07K14/4722

    摘要： The present invention provides a GTP binding protein containing the following amino acid sequence, with a molecular weight of about 22K dalton and having GTP binding activity which is inhibited by N-ethyl-maleimide and GTP hydrolyzing activity:Thr-Ile-Glu-Asp-Ser-Tyr, and a method for the production of a GTP binding protein, which comprises introducing a DNA fragment containing DNA that encodes the GTP binding protein into a cloning site present at the downstream to a promoter of an expression vector, then introducing the expression vector thus constructed into a host, culturing said host, thereby expressing and accumulating the GTP binding protein and then collecting thereof.

    摘要翻译： 本发明提供含有下列氨基酸序列的GTP结合蛋白，其分子量约为22K道尔顿，具有被N-乙基 - 马来酰亚胺抑制的GTP结合活性和GTP水解活性：Thr-Ile-Glu-Asp- Ser-Tyr和GTP结合蛋白的制造方法，其特征在于，将含有编码GTP结合蛋白的DNA的DNA片段导入到下游的克隆位点，形成表达载体的启动子， 载体如此构建成宿主，培养所述宿主，从而表达和积聚GTP结合蛋白，然后收集。

    Chondromodulin-I protein
    8.
    发明授权
    Chondromodulin-I protein 失效
    软骨调节素-I蛋白

    公开(公告)号：US5444157A

    公开(公告)日：1995-08-22

    申请号：US47033

    申请日：1993-04-13

    申请人： Fujio Suzuki ,  Yuji Hiraki ,  Hideho Tanaka ,  Akihito Kamizono ,  Jun Kondo ,  Yutaka Teranishi

    发明人： Fujio Suzuki ,  Yuji Hiraki ,  Hideho Tanaka ,  Akihito Kamizono ,  Jun Kondo ,  Yutaka Teranishi

    IPC分类号： A61K38/00 ,  C07K14/51 ,  C07K14/00

    CPC分类号： C07K14/51 ,  A61K38/00

    摘要： A novel chondromodulin-I protein having a molecular weight of about 26,000 dalton on SDS-PAGE and capable of stimulating the growth of chondrocytes with or without FGF and promoting the differential potency of said cells, a DNA encoding said protein, expression vector containing said DNA, a transformant capable of producing recombinant chondromodulin-I protein, a process for producing chondromodulin-I protein by culturing said transformant and a pharmaceutical composition containing chondromodulin-I protein as an active ingredient.

    摘要翻译： 一种在SDS-PAGE上具有约26,000道尔顿分子量的新型软骨调节素-I蛋白，能够刺激具有或不含FGF的软骨细胞的生长并促进所述细胞的差异效力，编码所述蛋白质的DNA，含有所述DNA的表达载体 ，能够产生重组软骨调节素-I蛋白的转化体，通过培养所述转化体生产软骨调节素-I蛋白的方法和含有软骨调节素-I蛋白作为活性成分的药物组合物。