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    BIOLOGICAL SUBSTANCE DETECTION METHOD
    3.
    发明申请
    BIOLOGICAL SUBSTANCE DETECTION METHOD 有权
    生物物质检测方法

    公开(公告)号：US20130157287A1

    公开(公告)日：2013-06-20

    申请号：US13819453

    申请日：2011-08-30

    申请人： Kensaku Takanashi ,  Hideki Gouda ,  Hisatake Okada ,  Yasushi Nakano ,  Kohsuke Gonda ,  Motohiro Takeda ,  Noriaki Ohuchi

    发明人： Kensaku Takanashi ,  Hideki Gouda ,  Hisatake Okada ,  Yasushi Nakano ,  Kohsuke Gonda ,  Motohiro Takeda ,  Noriaki Ohuchi

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6486 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/4833 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2021/6441

    摘要： A biological substance detection method for detecting a biological substance specifically in a pathological specimen, comprising a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen, with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.

    摘要翻译： 一种用于特异性检测病理标本中生物物质的生物物质检测方法，包括使用荧光标记对病理标本进行免疫染色的步骤，用形态学观察目的的染色试剂（曙红）染色病理标本的步骤，以观察 病理标本的形态，用激发光照射染色的病理标本的步骤，引起荧光发射并检测病理标本中的生物物质。 在免疫染色病理标本的步骤中，使用在与曙红的激发波长区域不同的区域中出现激发波长的特殊荧光粒子作为荧光标记。

    Method for Determining Effectiveness of Medicine Containing Antibody as Component
    8.
    发明申请
    Method for Determining Effectiveness of Medicine Containing Antibody as Component 审中-公开
    测定含有抗体作为成分的药物有效性的方法

    公开(公告)号：US20130230866A1

    公开(公告)日：2013-09-05

    申请号：US13822320

    申请日：2011-08-26

    申请人： Minoru Miyashita ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Motohiro Takeda

    发明人： Minoru Miyashita ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Motohiro Takeda

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6486 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/94 ,  G01N2800/52

    摘要： Protein recognized by an antibody used as an active ingredient of an antibody medicine such as trastuzumab or an antibody used for targeting a target site of an active ingredient is highly accurately quantitatively determined by employing a quantitative tissue staining method of biological tissues, thereby providing a method for determining therapeutic effectiveness of a medicine containing such an antibody as a component. The effectiveness of a medicine containing an antibody as a component is determined by employing a tissue staining method comprising the steps of: labeling the antibody in the medicine containing an antibody as a component with a fluorescent material and contacting the thus fluorescence-labeled antibody with a tissue sample; obtaining a fluorescence image by irradiating, with excitation light, a tissue site contacted with the antibody; obtaining an autofluorescence image in the same field of view and at the same focus as in the fluorescence image in a close region on a shorter wavelength side or a longer wavelength side of an acquisition wavelength region of fluorescence emitted by the fluorescent material; obtaining a corrected fluorescence image by performing image processing for removing fluorescence brightness of the autofluorescence image from fluorescence brightness of the fluorescence image; counting the number of cells in the tissue site contacted with the antibody; measuring average fluorescence brightness per fluorescent particle; and calculating the number of fluorescent particles per cell.

    摘要翻译： 通过使用生物组织的定量组织染色法，高度准确地定量地测定由用作抗体药物如曲妥珠单抗或用于靶向活性成分的靶位点的抗体的抗体识别的蛋白质，从而提供方法 用于确定含有作为组分的抗体的药物的治疗有效性。 含有抗体作为成分的药物的有效性通过使用组织染色法测定，该方法包括以下步骤：在含有抗体作为组分的药物中用荧光物质标记抗体，并将由此荧光标记的抗体与 组织样本 通过用激发光照射与抗体接触的组织部位来获得荧光图像; 在荧光材料发射的荧光的采集波长区域的较短波长侧或较长波长侧的接近区域中，以与荧光图像相同的视场和相同的焦点获得自发荧光图像; 通过进行从荧光图像的荧光亮度去除自发荧光图像的荧光亮度的图像处理来获得校正的荧光图像; 计数与抗体接触的组织部位中的细胞数; 测量每个荧光颗粒的平均荧光亮度; 并计算每个细胞的荧光颗粒数。

    METHOD FOR DETERMINING CANCER ONSET OR CANCER ONSET RISK
    10.
    发明申请
    METHOD FOR DETERMINING CANCER ONSET OR CANCER ONSET RISK 审中-公开
    用于确定癌症或癌症风险的方法

    公开(公告)号：US20130203082A1

    公开(公告)日：2013-08-08

    申请号：US13819008

    申请日：2011-08-26

    申请人： Kohsuke Gonda ,  Minoru Miyashita ,  Motohiro Takeda ,  Noriaki Ohuchi

    发明人： Kohsuke Gonda ,  Minoru Miyashita ,  Motohiro Takeda ,  Noriaki Ohuchi

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6486 ,  B82Y15/00 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/57415 ,  G01N33/588 ,  G01N2800/50

    摘要： A highly accurate and quantitative method for determining cancer onset or cancer onset risk by a quantitative tissue staining method in biological tissues using an antibody capable of recognizing a cancer growth regulatory factor or cancer metastasis regulatory factor such as PAR1 antibody, which inhibits the cancer cell mobility and infiltration is provided. Cancer onset or cancer onset risk is determined using the tissue staining method comprising the steps of: labeling an antibody which recognizes a cancer growth regulatory factor or cancer metastasis regulatory factor with a fluorescent material, and contacting the fluorescent-labeled antibody with a tissue sample; irradiating a tissue site in contact with the antibody with excitation light to acquire a fluorescence image; acquiring an autofluorescence image in a vicinity region of a short wavelength side or long wavelength side of an acquisition region of fluorescence wavelength emitted by the fluorescent material, in the same field of vision and in the same focal point as those of the fluorescence image; acquiring a corrected fluorescence image by image processing to eliminate a fluorescent brightness of the autofluorescence image from the fluorescent brightness of the fluorescence image; counting the number of cells at the tissue site in contact with the antibody; measuring a mean fluorescent brightness of a single fluorescent particle; and calculating the number of fluorescent particles per cell.

    摘要翻译： 使用能够识别癌细胞生长调节因子或癌转移调节因子（例如抑制癌细胞迁移率的PAR1抗体）的抗体，通过定量组织染色法在生物组织中测定癌症发病或癌症发病风险的高度准确和定量的方法 并提供渗透。 使用组织染色方法确定癌症发病或癌症发病风险，其包括以下步骤：用荧光材料标记识别癌症生长调节因子或癌症转移调节因子的抗体，并将荧光标记的抗体与组织样品接触; 用激发光照射与抗体接触的组织部位以获得荧光图像; 在荧光材料发射的荧光波长的获取区域的短波长侧或长波长侧的附近区域中，在与荧光图像相同的视场和相同的焦点中获取自发荧光图像; 通过图像处理获得校正的荧光图像，以从荧光图像的荧光亮度消除自发荧光图像的荧光亮度; 计数与抗体接触的组织部位的细胞数; 测量单个荧光颗粒的平均荧光亮度; 并计算每个细胞的荧光颗粒数。