SUPPORT FOR PROTEIN IMMOBILIZATION, IMMOBILIZED PROTEIN, AND METHODS FOR PRODUCING THE SAME
    1.
    发明申请
    SUPPORT FOR PROTEIN IMMOBILIZATION, IMMOBILIZED PROTEIN, AND METHODS FOR PRODUCING THE SAME 审中-公开
    蛋白质固定,固定化蛋白质的制备及其生产方法

    公开(公告)号:US20130109072A1

    公开(公告)日:2013-05-02

    申请号:US13702308

    申请日:2011-06-08

    IPC分类号: C12N11/14 B01J32/00 C12N11/02

    摘要: A support for enzyme immobilization is described, which is for immobilizing enzymes of various molecular sizes and also for, due to the modification of the surface silanol groups of porous silica particles, for immobilizing various kinds of enzymes, and enables the design of an immobilized enzyme, which exhibits an activity equivalent to that of the corresponding non-immobilized enzyme and withstands repeated use. A method for producing the support is also described. The support includes porous silica particles having an interparticle void structure therein, characterized in that the porous silica particles have a specific average particle size, a specific surface area, a specific pore volume, a specific pore size distribution and a specific porosity and have a substituent containing an organic group or an amino group on the surface thereof. An immobilized protein obtained by immobilizing a protein on the above support is also described.

    摘要翻译: 描述了酶固定的载体,其用于固定各种分子尺寸的酶,并且由于多孔二氧化硅颗粒的表面硅烷醇基团的修饰,用于固定各种酶,并且使得能够设计固定化酶 ,其表现出与相应的非固定化酶相当的活性并经受重复使用。 还描述了用于制备载体的方法。 载体包括其中具有颗粒间空隙结构的多孔二氧化硅颗粒,其特征在于多孔二氧化硅颗粒具有比表面积,比孔面积,比孔径,比孔径和比孔径,并具有取代基 在其表面含有有机基团或氨基。 还描述了通过将蛋白质固定在上述载体上获得的固定化蛋白质。