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    Fungal serine protease and use thereof
    5.
    发明申请
    Fungal serine protease and use thereof 有权
    真菌丝氨酸蛋白酶及其用途

    公开(公告)号：US20110028375A1

    公开(公告)日：2011-02-03

    申请号：US12799638

    申请日：2010-04-28

    申请人： Kari Juntunen ,  Leena Valtakari ,  Susanna Makinen ,  Jarno Kallio ,  Jari Vehmaanpera ,  Pentti Ojapalo ,  Marja Paloheimo ,  George Szakacs

    发明人： Kari Juntunen ,  Leena Valtakari ,  Susanna Makinen ,  Jarno Kallio ,  Jari Vehmaanpera ,  Pentti Ojapalo ,  Marja Paloheimo ,  George Szakacs

    IPC分类号： C11D3/386 ,  A23J3/34 ,  C12N9/58 ,  C12N9/62 ,  C12N1/00 ,  C12N1/15 ,  C12N1/02 ,  C12N15/63 ,  C07H21/00 ,  C11D3/60

    CPC分类号： C12N9/58 ,  C11D3/386 ,  C11D3/38681

    摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.

    摘要翻译： 本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：18的氨基酸序列的成熟Fa_RF7182酶的氨基酸序列。丝氨酸蛋白酶可从尖锐镰孢获得，更优选来自保藏菌株CBS 还公开了编码所述蛋白酶的核酸序列，例如包含以保藏号DSM 22208沉积在大肠杆菌RF7803中的核苷酸序列SEQ ID NO：12的质粒pALK2530和保藏的全长基因SEQ ID NO：13的质粒pALK2531 在保藏号DSM 22209的大肠杆菌RF7879中，以及真菌宿主如Trichoderm。 所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。

    Fungal serine protease and use thereof
    6.
    发明授权
    Fungal serine protease and use thereof 有权
    真菌丝氨酸蛋白酶及其用途

    公开(公告)号：US08609390B2

    公开(公告)日：2013-12-17

    申请号：US12799638

    申请日：2010-04-28

    申请人： Kari Juntunen ,  Leena Valtakari ,  Susanna Makinen ,  Jarno Kallio ,  Jari Vehmaanpera ,  Pentti Ojapalo ,  Marja Paloheimo ,  George Szakacs

    发明人： Kari Juntunen ,  Leena Valtakari ,  Susanna Makinen ,  Jarno Kallio ,  Jari Vehmaanpera ,  Pentti Ojapalo ,  Marja Paloheimo ,  George Szakacs

    IPC分类号： C12N9/68

    CPC分类号： C12N9/58 ,  C11D3/386 ,  C11D3/38681

    摘要： The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.

    摘要翻译： 本发明涉及真菌丝氨酸蛋白酶，其包含具有SEQ ID NO：18的氨基酸序列的成熟Fa_RF7182酶的氨基酸序列。丝氨酸蛋白酶可从尖锐镰孢获得，更优选来自保藏菌株CBS 还公开了编码所述蛋白酶的核酸序列，例如包含以保藏号DSM 22208沉积在大肠杆菌RF7803中的核苷酸序列SEQ ID NO：12的质粒pALK2530和保藏的全长基因SEQ ID NO：13的质粒pALK2531 在保藏号DSM 22209的大肠杆菌RF7879中，以及真菌宿主如Trichoderm。 所述蛋白酶可用作适用于洗涤剂组合物和用于处理纤维，用于处理毛发，用于处理皮革，用于处理食品或饲料，或用于任何涉及蛋白质材料的改性，降解或去除的任何应用的酶制剂。