公开(公告)号：US20050239091A1

公开(公告)日：2005-10-27

申请号：US10885949

申请日：2004-07-08

申请人： Matthew Collis ,  Thomas Fort

发明人： Matthew Collis ,  Thomas Fort

IPC分类号： C12N15/10 ,  C12Q1/68 ,  G01N35/00

CPC分类号： C12N15/1013 ,  G01N35/0098 ,  G01N35/0099

摘要： A method includes creating a mixture in a container, the mixture including a sample containing at least one molecule of nucleic acid, at least one magnetically-responsive particle, and a remainder; and providing the mixture with a pH of less than about 7.0, thereby altering the surface charge properties of the at least one magnetically-responsive particle. The alteration in the surface change properties of the at least one magnetically-responsive particle causes the at least one molecule of nucleic acid to become non-specifically bound to the at least one magnetically-responsive particle to form a complex. Preferably, the at least one magnetically-responsive particle is uncoated, untreated, lacks surface modification, and has a diameter of about 0.1 μm to about 1.0 μm.

摘要翻译： 一种方法包括在容器中产生混合物，所述混合物包括含有至少一个核酸分子的样品，至少一个磁响应颗粒和其余部分; 以及提供小于约7.0的pH的混合物，从而改变至少一个磁响应颗粒的表面电荷性质。 所述至少一个磁响应颗粒的表面变化特性的改变导致所述至少一个核酸分子变得非特异性结合至所述至少一个磁响应颗粒以形成复合物。 优选地，所述至少一个磁响应颗粒是未涂覆的，未经处理的，缺少表面改性，并且具有约0.1μm至约1.0μm的直径。

公开(公告)号：US20060030056A1

公开(公告)日：2006-02-09

申请号：US11195657

申请日：2005-08-03

申请人： Thomas Fort ,  Matthew Collis

发明人： Thomas Fort ,  Matthew Collis

IPC分类号： G01N33/551

CPC分类号： C07K1/14 ,  G01N33/54326 ,  G01N33/54333

摘要： A method useful for the reversible binding of a protein molecule in a biological sample. The method uses paramagnetic particles having an associated electronic charge to bind proteins with the opposite charge to form a particle/protein complex. The complex can be immobilized to a container wall by applying a magnetic field to the particle/protein complex. The sample may be further processed to obtain a protein sample in a more pure form or a sample depleted of select proteins.

摘要翻译： 可用于生物样品中蛋白质分子可逆结合的方法。 该方法使用具有相关电子电荷的顺磁性颗粒来结合具有相反电荷的蛋白质以形成颗粒/蛋白质复合物。 通过向颗粒/蛋白质复合物施加磁场，可将复合物固定在容器壁上。 可以进一步处理样品以获得更纯的形式的蛋白质样品或贫化的选择蛋白质的样品。

公开(公告)号：US20070031880A1

公开(公告)日：2007-02-08

申请号：US11544040

申请日：2006-10-06

申请人： Jianrong Lou ,  Matthew Collis ,  Thomas Fort

发明人： Jianrong Lou ,  Matthew Collis ,  Thomas Fort

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/1013 ,  C07H21/04

摘要： A composition and method for the purification of nucleic acid are disclosed. The composition includes at least one alkaline agent and at least one detergent. The composition preferably also includes a suspension of paramagnetic particles and an acidic solution. The method involves the use of the composition with paramagnetic particles to extract nucleic acid from a biological sample.

摘要翻译： 公开了一种用于纯化核酸的组合物和方法。 该组合物包括至少一种碱性试剂和至少一种洗涤剂。 组合物优选还包括顺磁性颗粒和酸性溶液的悬浮液。 该方法涉及使用具有顺磁性颗粒的组合物从生物样品中提取核酸。

公开(公告)号：US20060084089A1

公开(公告)日：2006-04-20

申请号：US11195656

申请日：2005-08-03

申请人： Thomas Fort ,  Matthew Collis ,  Thomas Gentle

发明人： Thomas Fort ,  Matthew Collis ,  Thomas Gentle

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/551

CPC分类号： B01D15/3828 ,  B01D15/361 ,  B01D15/3885 ,  B01J20/28009 ,  B01J20/3242 ,  B01J20/3274 ,  C12N15/1013 ,  C12Q1/6806 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/6848 ,  C12Q2563/149 ,  C12Q2563/143

摘要： Methods for isolating a compound from a multipart, typically biological sample. The methods use at least one paramagnetic particle having an associated electronic charge to bind compounds with the opposite charge to form a particle/compound complex. Alternatively, the paramagnetic particles have a ligand or functional group with an affinity for a target compound to form a particle/compound complex. The complex can be immobilized by applying a magnetic field to the particle/protein complex. The sample may be further processed to obtain a protein sample in a more pure form or a sample depleted of select compounds.

摘要翻译： 从多部分（通常为生物样品）分离化合物的方法。 所述方法使用至少一个具有相关电子电荷的顺磁性粒子来结合具有相反电荷的化合物以形成颗粒/复合物复合物。 或者，顺磁性颗粒具有对目标化合物具有亲和性以形成颗粒/复合物复合物的配体或官能团。 可以通过向颗粒/蛋白质复合物施加磁场来固定复合物。 可以进一步处理样品以获得更纯的形式的蛋白质样品或贫化的选择化合物的样品。

公开(公告)号：US20050233332A1

公开(公告)日：2005-10-20

申请号：US10825943

申请日：2004-04-14

申请人： Matthew Collis

发明人： Matthew Collis

IPC分类号： C12N15/09 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6816 ,  C12Q2565/1025

摘要： A detector oligonucleotide comprises multiple pairs of a donor fluorophore and a quencher molecule, which donor fluorophores and quencher molecules are separated by a site that is capable of being cleaved when in double-stranded form. The detector oligonucleotide may be made double-stranded in a manner that depends on the presence of a target nucleic acid, allowing the cleavage sites to be cleaved. Separation of the donor fluorophores and the quencher molecules decreases fluorescence quenching and generates a detectable change in a fluorescence parameter of the fluorophores of the detector oligonucleotide. By using multiple donor/quencher pairs, the present detector oligonucleotide advantageously generates a high signal to noise ratio and high efficiency in detection of a target nucleic acid.

摘要翻译： 检测器寡核苷酸包含多对供体荧光团和猝灭剂分子，供体荧光团和猝灭剂分子由能双链形式切割的位点分开。 检测器寡核苷酸可以以取决于靶核酸的存在的方式制成双链，允许切割位点被切割。 供体荧光团和猝灭剂分子的分离会降低荧光猝灭，并产生检测器寡核苷酸的荧光团的荧光参数的可检测变化。 通过使用多个供体/猝灭剂对，本发明的检测器寡核苷酸有利地产生高的信噪比并且在靶核酸的检测中具有高效率。

公开(公告)号：US08940882B2

公开(公告)日：2015-01-27

申请号：US11785441

申请日：2007-04-18

申请人： Matthew Collis

发明人： Matthew Collis

IPC分类号： C07H21/04 ,  C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q2565/1025

摘要： A detector oligonucleotide comprises multiple pairs of a donor fluorophore and a quencher molecule, which donor fluorophores and quencher molecules are separated by a site that is capable of being cleaved when in double-stranded form. The detector oligonucleotide may be made double-stranded in a manner that depends on the presence of a target nucleic acid, allowing the cleavage sites to be cleaved. Separation of the donor fluorophores and the quencher molecules decreases fluorescence quenching and generates a detectable change in a fluorescence parameter of the fluorophores of the detector oligonucleotide. By using multiple donor/quencher pairs, the present detector oligonucleotide advantageously generates a high signal to noise ratio and high efficiency in detection of a target nucleic acid.

摘要翻译： 检测器寡核苷酸包含多对供体荧光团和猝灭剂分子，供体荧光团和猝灭剂分子由能双链形式切割的位点分开。 检测器寡核苷酸可以以取决于靶核酸的存在的方式制成双链，允许切割位点被切割。 供体荧光团和猝灭剂分子的分离会降低荧光猝灭，并产生检测器寡核苷酸的荧光团的荧光参数的可检测变化。 通过使用多个供体/猝灭剂对，本发明的检测器寡核苷酸有利地产生高的信噪比并且在靶核酸的检测中具有高效率。

公开(公告)号：US20090061497A1

公开(公告)日：2009-03-05

申请号：US12165069

申请日：2008-06-30

申请人： Matthew Collis ,  Michael Lizzi

发明人： Matthew Collis ,  Michael Lizzi

IPC分类号： C12N13/00 ,  C07H21/04

CPC分类号： C12N15/1013 ,  C12Q1/6806 ,  G01N33/54333

摘要： A method is provided for extracting and purifying components of biological samples with a two-step process for elution and neutralization of the components from the sample. The separate elution and neutralization steps use adjustment of the buffer pH to improve extraction and purification of the desired components.

摘要翻译： 提供了用于从样品中洗脱和中和组分的两步法提取和纯化生物样品组分的方法。 单独的洗脱和中和步骤使用缓冲液pH的调节来改善所需组分的提取和纯化。