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    Extraction of nucleic acids using small diameter magnetically-responsive particles
    1.
    发明申请
    Extraction of nucleic acids using small diameter magnetically-responsive particles 审中-公开
    使用小直径磁响应颗粒提取核酸

    公开(公告)号：US20050239091A1

    公开(公告)日：2005-10-27

    申请号：US10885949

    申请日：2004-07-08

    申请人： Matthew Collis ,  Thomas Fort

    发明人： Matthew Collis ,  Thomas Fort

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  G01N35/00

    CPC分类号： C12N15/1013 ,  G01N35/0098 ,  G01N35/0099

    摘要： A method includes creating a mixture in a container, the mixture including a sample containing at least one molecule of nucleic acid, at least one magnetically-responsive particle, and a remainder; and providing the mixture with a pH of less than about 7.0, thereby altering the surface charge properties of the at least one magnetically-responsive particle. The alteration in the surface change properties of the at least one magnetically-responsive particle causes the at least one molecule of nucleic acid to become non-specifically bound to the at least one magnetically-responsive particle to form a complex. Preferably, the at least one magnetically-responsive particle is uncoated, untreated, lacks surface modification, and has a diameter of about 0.1 μm to about 1.0 μm.

    摘要翻译： 一种方法包括在容器中产生混合物，所述混合物包括含有至少一个核酸分子的样品，至少一个磁响应颗粒和其余部分; 以及提供小于约7.0的pH的混合物，从而改变至少一个磁响应颗粒的表面电荷性质。 所述至少一个磁响应颗粒的表面变化特性的改变导致所述至少一个核酸分子变得非特异性结合至所述至少一个磁响应颗粒以形成复合物。 优选地，所述至少一个磁响应颗粒是未涂覆的，未经处理的，缺少表面改性，并且具有约0.1μm至约1.0μm的直径。

    Chemical treatment of biological samples for nucleic acid extraction and kits therefor
    2.
    发明申请
    Chemical treatment of biological samples for nucleic acid extraction and kits therefor 审中-公开
    用于核酸提取的生物样品的化学处理及其试剂盒

    公开(公告)号：US20070031880A1

    公开(公告)日：2007-02-08

    申请号：US11544040

    申请日：2006-10-06

    申请人： Jianrong Lou ,  Matthew Collis ,  Thomas Fort

    发明人： Jianrong Lou ,  Matthew Collis ,  Thomas Fort

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12N15/1013 ,  C07H21/04

    摘要： A composition and method for the purification of nucleic acid are disclosed. The composition includes at least one alkaline agent and at least one detergent. The composition preferably also includes a suspension of paramagnetic particles and an acidic solution. The method involves the use of the composition with paramagnetic particles to extract nucleic acid from a biological sample.

    摘要翻译： 公开了一种用于纯化核酸的组合物和方法。 该组合物包括至少一种碱性试剂和至少一种洗涤剂。 组合物优选还包括顺磁性颗粒和酸性溶液的悬浮液。 该方法涉及使用具有顺磁性颗粒的组合物从生物样品中提取核酸。

    Use of magnetic material to fractionate samples
    4.
    发明申请
    Use of magnetic material to fractionate samples 审中-公开
    使用磁性材料分离样品

    公开(公告)号：US20060030056A1

    公开(公告)日：2006-02-09

    申请号：US11195657

    申请日：2005-08-03

    申请人： Thomas Fort ,  Matthew Collis

    发明人： Thomas Fort ,  Matthew Collis

    IPC分类号： G01N33/551

    CPC分类号： C07K1/14 ,  G01N33/54326 ,  G01N33/54333

    摘要： A method useful for the reversible binding of a protein molecule in a biological sample. The method uses paramagnetic particles having an associated electronic charge to bind proteins with the opposite charge to form a particle/protein complex. The complex can be immobilized to a container wall by applying a magnetic field to the particle/protein complex. The sample may be further processed to obtain a protein sample in a more pure form or a sample depleted of select proteins.

    摘要翻译： 可用于生物样品中蛋白质分子可逆结合的方法。 该方法使用具有相关电子电荷的顺磁性颗粒来结合具有相反电荷的蛋白质以形成颗粒/蛋白质复合物。 通过向颗粒/蛋白质复合物施加磁场，可将复合物固定在容器壁上。 可以进一步处理样品以获得更纯的形式的蛋白质样品或贫化的选择蛋白质的样品。

    Use of an extraction control in a method of extracting nucleic acids
    5.
    发明申请
    Use of an extraction control in a method of extracting nucleic acids 有权
    在提取核酸的方法中使用提取控制

    公开(公告)号：US20060014170A1

    公开(公告)日：2006-01-19

    申请号：US11114952

    申请日：2005-04-25

    申请人： Tobin Hellyer ,  Thomas Fort ,  Ray McMillian

    发明人： Tobin Hellyer ,  Thomas Fort ,  Ray McMillian

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12Q1/689 ,  C12Q2545/114 ,  C12Q2531/113 ,  C12Q2545/101

    摘要： The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.

    摘要翻译： 本发明涉及确保来自核酸生物样品的提取处理的有效性的方法。 本发明的方法能够区分来自样品的核酸提取的可能缺陷以及随后扩增步骤中的可能缺陷。 本发明还涉及用于从生物样品中提取核酸的包装阵列。

    Use of an extraction control in a method of extracting nucleic acids
    9.
    发明授权
    Use of an extraction control in a method of extracting nucleic acids 有权
    在提取核酸的方法中使用提取控制

    公开(公告)号：US07371531B2

    公开(公告)日：2008-05-13

    申请号：US11114952

    申请日：2005-04-25

    申请人： Tobin Hellyer ,  Thomas Fort ,  Ray A. McMillian

    发明人： Tobin Hellyer ,  Thomas Fort ,  Ray A. McMillian

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/6806 ,  C12Q1/689 ,  C12Q2545/114 ,  C12Q2531/113 ,  C12Q2545/101

    摘要： The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.

    摘要翻译： 本发明涉及确保来自核酸生物样品的提取处理的有效性的方法。 本发明的方法能够区分来自样品的核酸提取的可能缺陷以及随后扩增步骤中的可能缺陷。 本发明还涉及用于从生物样品中提取核酸的包装阵列。

    Filled pastry
    10.
    发明申请
    Filled pastry 审中-公开
    馅饼

    公开(公告)号：US20060073239A1

    公开(公告)日：2006-04-06

    申请号：US10957411

    申请日：2004-10-01

    申请人： Janet Boyle ,  Carolina Hoxie ,  Thomas Fort

    发明人： Janet Boyle ,  Carolina Hoxie ,  Thomas Fort

    IPC分类号： A23L1/00

    CPC分类号： A21D2/181 ,  A21D13/02 ,  A21D13/19 ,  A21D13/31 ,  A21D13/60 ,  A21D15/02 ,  A23L5/00 ,  A23L7/00 ,  A23L7/122 ,  A23L21/00 ,  A23L25/10 ,  A23L29/30 ,  A23V2002/00 ,  A23V2200/08

    摘要： A filled pastry having a cooked dough shell and a filling. The cooked dough shell is made from raw dough that includes whole-wheat flour, wheat bran, sweetener, shortening, and water and can have an appearance and flavor that is similar to graham crackers. The filling is prepared from a material that is distinct from the dough shell. The filling is substantially retained within the dough shell. An interface between the dough shell and the filling minimizes moisture migration.

    摘要翻译： 一个馅饼，一个煮熟的面团和馅料。 煮熟的面团外壳由生面团制成，包括全麦面粉，麦麸，甜味剂，起酥油和水，并且可以具有类似于格拉汉姆饼干的外观和风味。 填料由与面团外壳不同的材料制成。 填充物基本上保持在面团外壳内。 面团外壳和填充物之间的界面使水分迁移最小化。