公开(公告)号：US20240294897A1

公开(公告)日：2024-09-05

申请号：US18657335

申请日：2024-05-07

Applicant: BIOMAGNETIC SOLUTIONS LLC

Inventor： Paul A. LIBERTI ,  Todor R. KHRISTOV ,  Dustin W. RITTER

IPC: C12N13/00 ,  A61M1/36 ,  B01D15/38 ,  B01F31/23 ,  B01F33/452 ,  B03C1/01 ,  B03C1/033 ,  B03C1/034 ,  B03C1/28 ,  G01N33/543

CPC classification number: C12N13/00 ,  A61M1/3618 ,  B01D15/3885 ,  B01F31/23 ,  B01F33/452 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/034 ,  B03C1/288 ,  G01N33/54326 ,  B03C2201/18 ,  B03C2201/22 ,  B03C2201/26

Abstract: An apparatus and methods are provided for the magnetic separation of target bioentities. The apparatus includes a fluid chamber and a magnetic clement for drawing target bioentities toward a collection surface of the fluid chamber. The apparatus may include a positioning assembly operable to variable change the position and orientation of the fluid chamber relative to the magnetic element.

公开(公告)号：US12023603B2

公开(公告)日：2024-07-02

申请号：US17026177

申请日：2020-09-19

Applicant: University of Utah Research Foundation

Inventor： Marc David Porter ,  Robert Joseph Soto ,  Mark Andrew Hayes

IPC: B01D15/38 ,  B01D15/22 ,  G01N30/02 ,  G01N30/60

CPC classification number: B01D15/3885 ,  B01D15/22 ,  G01N30/6052 ,  G01N2030/027

Abstract: This invention discloses a design a capillary column for use with electrochemically modulated liquid chromatography (EMLC). The capillary design, which results in a marked reduction in the flow of current through the column, enables the use of a two-electrode column construction that overcomes the mechanical and electrical shortfalls of the conventional standard bore design.

公开(公告)号：US20230176062A1

公开(公告)日：2023-06-08

申请号：US18083279

申请日：2022-12-16

Applicant: Miltenyi Biotec B.V. & Co. KG

Inventor： Stefan Miltenyi ,  Winfried Schimmelpfennig ,  Holger Lantow ,  Niklas Elmar Neuschaefer ,  Martin Biehl ,  Eiad Kabaha ,  Juergen Schulz

IPC: G01N33/58 ,  A61M1/36 ,  B03C1/28 ,  B04B5/04 ,  B04B13/00 ,  B03C1/032 ,  B03C1/00 ,  B03C1/027 ,  B03C1/033 ,  B03C1/034 ,  A61M1/02 ,  G01N35/00 ,  C12N5/00 ,  C12M1/26 ,  C12M1/00 ,  B01D21/26 ,  B04B5/00 ,  B04B5/10 ,  B04B1/12 ,  B04B7/08 ,  B01D15/38 ,  C12N5/0789 ,  C12N5/077 ,  C12N5/0783 ,  C12M1/36

CPC classification number: G01N33/58 ,  A61M1/0209 ,  A61M1/3618 ,  A61M1/3633 ,  A61M1/3679 ,  A61M1/3692 ,  A61M1/3693 ,  A61M1/3696 ,  B01D15/3809 ,  B01D15/3885 ,  B01D21/26 ,  B03C1/002 ,  B03C1/027 ,  B03C1/032 ,  B03C1/034 ,  B03C1/288 ,  B03C1/0332 ,  B03C1/0335 ,  B04B1/12 ,  B04B5/00 ,  B04B5/04 ,  B04B5/10 ,  B04B5/0442 ,  B04B7/08 ,  B04B13/00 ,  C12M23/28 ,  C12M33/10 ,  C12M41/48 ,  C12M47/04 ,  C12N5/0018 ,  C12N5/0636 ,  C12N5/0646 ,  C12N5/0647 ,  C12N5/0669 ,  G01N35/0098 ,  B01L3/5021 ,  B03C2201/18 ,  B03C2201/26

Abstract: The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

公开(公告)号：US09777075B2

公开(公告)日：2017-10-03

申请号：US15064815

申请日：2016-03-09

Applicant: Lab-on-a-bead AB

Inventor： Sven Oscarsson ,  Kristofer Eriksson ,  Peter Svedlindh

IPC: G01N33/548 ,  C08B37/00 ,  B01J20/285 ,  B01J20/286 ,  B01J20/32 ,  B01J20/28 ,  B01J20/24 ,  G01N33/543 ,  B01D15/38

CPC classification number: C08B37/0039 ,  B01D15/3804 ,  B01D15/3885 ,  B01J20/24 ,  B01J20/28009 ,  B01J20/28016 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3242 ,  B01J20/3265 ,  G01N33/5434 ,  G01N33/548

Abstract: A novel system for the analysis of molecules and cells, comprising clusters where a non-magnetic particle is supplemented with magnetic particles to form a characteristic pattern, fingerprint or bar code. Methods and devices for formation of such particles are also disclosed.

公开(公告)号：US20170241946A1

公开(公告)日：2017-08-24

申请号：US15310069

申请日：2015-06-04

Applicant: The Regents of the University of California

Inventor： Amy E. Herr ,  Todd A. Duncombe

IPC: G01N27/447 ,  B01D15/38 ,  B01J20/285 ,  B01D15/20

CPC classification number: G01N27/44739 ,  B01D15/20 ,  B01D15/3871 ,  B01D15/3876 ,  B01D15/3885 ,  B01D17/06 ,  B01J20/267 ,  B01J20/285 ,  G01N27/44726 ,  G01N27/44734 ,  G01N27/44747 ,  G01N27/44756 ,  G01N2030/525

Abstract: Provided are devices that include a polymeric separation medium configured to immobilize one or more constituents of interest in the polymeric separation medium and have an increased pore size upon application of an applied stimulus. Systems including the devices, as well as methods of using the devices, are also provided. Embodiments of the present disclosure find use in a variety of different applications, including detecting whether an analyte is present in a fluid sample.

公开(公告)号：US20150299248A1

公开(公告)日：2015-10-22

申请号：US14441129

申请日：2013-11-11

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Jean-Luc Maloisel ,  Gustav Rodrigo ,  Bjorn Noren ,  Virendra Kumbhar

IPC: C07K1/16 ,  C07K1/36 ,  B01J20/289 ,  B01J20/26 ,  B01J20/28

CPC classification number: C07K1/165 ,  B01D15/3847 ,  B01D15/3885 ,  B01J20/265 ,  B01J20/28016 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3253 ,  B01J20/3255 ,  B01J20/3285 ,  B01J41/20 ,  B01J2220/80 ,  C07K1/36 ,  G01N33/544 ,  B01D15/363 ,  B01D15/327

Abstract: The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R1-L1-N(R3)-L2-R, immobilized on a support, wherein R1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L1 is either a methylene group or a covalent bond; R2 is a five- or six-membered, substituted or non-substituted ring structure; L2 is either a methylene group or a covalent bond; R3 is a methyl group; and wherein if R1 is a hydroxyethyl group and L1 is a covalent bond, R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

Abstract translation: 本发明公开了一种分离基质，其包含固定在载体上的式R1-L1-N（R3）-L2-R定义的多个分离配体，其中R1是五或六元的取代或非取代的 取代的环结构或羟乙基或羟丙基; L1是亚甲基或共价键; R2是五或六元，取代或未取代的环结构; L2是亚甲基或共价键; R3是甲基; 并且其中如果R 1是羟乙基并且L 1是共价键，则R 2是取代的芳环结构或取代或未取代的脂族环结构。

公开(公告)号：US20150185124A1

公开(公告)日：2015-07-02

申请号：US14658865

申请日：2015-03-16

Applicant: MagnaMedics GmbH

Inventor： Viorel Rusu ,  Sven Goethel

IPC: G01N1/34

CPC classification number: G01N1/34 ,  B01D15/3885 ,  C07K1/22 ,  Y10T436/25125

Abstract: The disclosure relates to a method for the selective separation of proteins from liquid biological materials which, based on the total quantity, contain a small proportion of one or more trace components, by means of the addition of polar organic solvents having a dipole moment in the range from 1.6 to 4.0 Debye, and adsorption of the proteins on a solid phase carrier, wherein the trace components are separated from the proteins bound to the solid phase carrier by adsorption by means of a) magnetic field, b) centrifugal force, c) gravitational force or d) compressive force, and the trace components remain in the liquid.

Abstract translation: 本公开涉及一种从液体生物材料中选择性分离蛋白质的方法，其通过在极性有机溶剂中加入极性有机溶剂，基于总量，含有少量的一种或多种痕量组分 范围为1.6至4.0德拜，并且将蛋白质吸附在固相载体上，其中通过借助a）磁场吸附将痕量组分与结合至固相载体的蛋白质分离，b）离心力，c） 重力或d）压缩力，痕量成分保留在液体中。

公开(公告)号：US20150045470A1

公开(公告)日：2015-02-12

申请号：US14383322

申请日：2013-03-05

Applicant: JSR CORPORATION

Inventor： Satoshi Katayose

IPC: C07K1/22 ,  B01J20/28 ,  B01J20/26 ,  C07K16/00

CPC classification number: C07K1/22 ,  B01D15/3885 ,  B01D15/424 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28009 ,  B01J20/28016 ,  B01J20/28095 ,  C07K16/00 ,  C07K16/065

Abstract: An antibody purification method includes: bringing a solution that includes an antibody and has a pH of 3.0 or more and less than 5.6 into contact with a carrier that includes a 2,3-dihydroxypropyl group to adsorb the antibody on the carrier; and bringing the carrier that has been brought into contact with the solution, into contact with an eluent having a pH of 5.6 or more and less than 10 to elute the antibody from the carrier.

Abstract translation: 抗体纯化方法包括：使包含抗体的溶液与pH3.0以上且小于5.6接触，与包含2,3-二羟丙基的载体接触以将抗体吸附在载体上; 并使已经与溶液接触的载体与pH为5.6以上且小于10的洗脱液接触，从载体洗脱抗体。

公开(公告)号：US20140227766A1

公开(公告)日：2014-08-14

申请号：US14124706

申请日：2012-06-01

Applicant: Peter Stanley Gagnon

Inventor： Peter Stanley Gagnon

IPC: C07K14/75 ,  C07K14/755 ,  C12N7/00 ,  C07K16/06

CPC classification number: C07K14/75 ,  B01D15/08 ,  B01D15/166 ,  B01D15/1807 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3885 ,  C07K1/16 ,  C07K14/755 ,  C07K16/00 ,  C07K16/065 ,  C12N7/00 ,  C12N2795/00 ,  C12N2795/14151

Abstract: Materials and methods for use of constrained cohydration agents in the purification of biological materials such as antibodies, viruses, cells, and cellular organelles in connection with convective chromatography, fluidized bed or co-precipitation applications.

Abstract translation: 在与对流色谱法，流化床或共沉淀应用有关的生物材料如抗体，病毒，细胞和细胞器的纯化中使用受限制的辅助水化剂的材料和方法。

公开(公告)号：US20140212961A1

公开(公告)日：2014-07-31

申请号：US14201744

申请日：2014-03-07

Applicant: Miltenyi Biotec GmbH

Inventor： Stefan Miltenyi ,  Winfried Schimmelpfennig ,  Holger Lantow ,  Niklas Elmar Neuschafer ,  Martin Biehl ,  Eiad Kabaha ,  Jürgen Schulz

IPC: C12M1/26

CPC classification number: G01N33/58 ,  A61M1/0209 ,  A61M1/3618 ,  A61M1/3633 ,  A61M1/3679 ,  A61M1/3692 ,  A61M1/3693 ,  A61M1/3696 ,  A61M2202/0071 ,  A61M2202/0413 ,  A61M2202/0427 ,  A61M2202/10 ,  B01D15/3809 ,  B01D15/3885 ,  B01D21/26 ,  B03C1/002 ,  B03C1/027 ,  B03C1/032 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/034 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  B04B1/12 ,  B04B5/00 ,  B04B5/04 ,  B04B5/0442 ,  B04B5/10 ,  B04B7/08 ,  B04B13/00 ,  B04B2005/0464 ,  B04B2013/006 ,  C12M23/28 ,  C12M33/10 ,  C12M41/48 ,  C12M47/04 ,  C12N5/0636 ,  C12N5/0646 ,  C12N5/0647 ,  C12N5/0669 ,  G01N35/0098 ,  G01N2333/70596

Abstract: The invention relates to a centrifuge for separating a sample into at least two components, comprising a chamber for receiving a sample to be centrifuged. According to the invention, the centrifuge further comprises a means for controlling the progress of the sample separation is located at the chamber.

Abstract translation: 本发明涉及一种用于将样品分离成至少两个组分的离心机，包括用于接收要离心的样品的室。 根据本发明，离心机还包括用于控制样品分离进度位于腔室的装置。