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    Laser microdissection apparatus and method
    1.
    发明授权
    Laser microdissection apparatus and method 有权
    激光显微解剖设备及方法

    公开(公告)号：US08715955B2

    公开(公告)日：2014-05-06

    申请号：US11222281

    申请日：2005-09-08

    Applicant: Brian W. Donovan ,  Thomas M. Baer

    Inventor： Brian W. Donovan ,  Thomas M. Baer

    IPC: G01N1/28

    CPC classification number: G01N1/28 ,  G01N1/2813 ,  G01N1/286 ,  G01N1/30 ,  G01N1/44 ,  G01N2001/2826 ,  G01N2001/2833 ,  G01N2001/284 ,  G01N2001/2886 ,  Y10T156/10 ,  Y10T156/1054

    Abstract: Systems and methods for automated laser microdissection are disclosed. In one variation, targeted biological material is manually or automatically selected and a transfer film is placed in juxtaposition to the location of an interior of a cut path. In another variation, a sample of biological material is mounted onto a polymer membrane which is then placed onto a substrate. Targeted biological material is manually or automatically selected and a transfer film is placed in juxtaposition with the targeted biological material on the side of the biological material. In yet another variation, a sample of biological material is mounted onto a polymer membrane which is then inverted onto a substrate. Targeted biological material is manually or automatically selected and a transfer film is placed in juxtaposition with the targeted biological material on the side of the polymer membrane. Then, an UV laser cuts along a cut path around the targeted portions of biological material in a closed cut path or a substantially closed cut path defining an interior and an exterior portion of the tissue sample. In a substantially closed cut path, bridges are left spanning the interior of the cut path and the exterior of the cut path. An IR laser activates at least a portion of the transfer film such that the transfer film in the vicinity of targeted portion adheres to the biological material interior to the cut path. The transfer film is then removed separating the targeted portions of biological material which are adhered to the transfer film from the remaining portion of the tissue sample.

    Abstract translation: 公开了用于自动激光显微切割的系统和方法。 在一个变型中，手动或自动选择目标生物材料，并且将转移膜并排放置在切割路径的内部的位置。 在另一个变体中，将生物材料样品安装在聚合物膜上，然后将其放置在基底上。 手动或自动选择靶向生物材料，并将转移膜与生物材料侧面上的靶向生物材料并置放置。 在另一个变体中，将生物材料样品安装在聚合物膜上，然后将其反转到基底上。 手动或自动选择目标生物材料，并将转移膜与聚合物膜侧面上的目标生物材料并置放置。 然后，UV激光沿着围绕生物材料的目标部分的切割路径沿封闭的切割路径或限定组织样本的内部和外部部分的基本封闭的切割路径切割。 在基本封闭的切割路径中，跨越切割路径的内部和切割路径的外部。 红外激光激活转印膜的至少一部分，使得目标部分附近的转印膜附着在生物材料内部至切割路径。 然后除去转移膜，从生物材料的目标部分分离出粘附到转移膜上的剩余部分的组织样品。

    Consumable for laser capture microdissection
    3.
    发明授权
    Consumable for laser capture microdissection 失效
    可用于激光捕获显微切割

    公开(公告)号：US07075640B2

    公开(公告)日：2006-07-11

    申请号：US08984979

    申请日：1997-12-04

    Applicant: Thomas M. Baer ,  David F. Head ,  John Toeppen

    Inventor： Thomas M. Baer ,  David F. Head ,  John Toeppen

    IPC: G01N21/01

    CPC classification number: G01N1/2813 ,  G01N2001/028 ,  G01N2001/2833 ,  G01N2001/284

    Abstract: Systems and methods for acquiring laser capture microdissection samples are disclosed. An integral portion of a biological reaction vessel includes a transfer film carrier having a substrate surface; and a laser capture microdissection transfer film coupled to the substrate surface of the transfer film carrier. The systems and methods facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

    Abstract translation: 公开了用于获取激光捕获显微解剖样品的系统和方法。 生物反应容器的整体部分包括具有基底表面的转移膜载体; 以及耦合到转印膜载体的基板表面的激光捕获显微切割转印膜。 系统和方法有助于快速准确的激光捕获显微切割，同时最大限度地减少污染。

    Laser capture microdissection analysis vessel
    8.
    发明授权
    Laser capture microdissection analysis vessel 失效
    激光捕获显微解剖分析容器

    公开(公告)号：US5859699A

    公开(公告)日：1999-01-12

    申请号：US797026

    申请日：1997-02-07

    Applicant: Thomas M. Baer ,  David F. Head

    Inventor： Thomas M. Baer ,  David F. Head

    IPC: G01N33/48 ,  B01L3/00 ,  B01L3/14 ,  G01N1/00 ,  G01N1/02 ,  G01N1/28 ,  G02B21/34 ,  G01N1/10

    CPC classification number: B01L3/50853 ,  B01L3/50825 ,  G01N1/2813 ,  G02B21/34 ,  B01L2300/021 ,  B01L2300/047 ,  G01N2001/284

    Abstract: A laser capture microdissection lift-off substrate comprising a transparent thermoplastic film is incorporated into substrate which may take the form of a cap or other structure associated with an analysis vessel or which may be used with a holder or other element of a LCM tissue sample transfer apparatus. The thermoplastic lift-off substrate film is held in a fixed position on the substrate which may be relative to a feature of a biological analysis vessel. The film may be affixed to a substrate which is a structure which goes into the vessel such that the sample is held in a fixed position relative to an observation port on the vessel.

    Abstract translation: 将包含透明热塑性薄膜的激光捕获显微切割剥离基底结合到基底中，基底可以采取与分析容器相关联的帽或其它结构的形式，或者可以与LCM组织样品转移的保持器或其他元件一起使用 仪器。 热塑性剥离基材膜保持在基板上的固定位置，其可以相对于生物分析容器的特征。 该膜可以固定到基底上，该基底是进入血管的结构，使得样品相对于血管上的观察孔保持在固定位置。

    Miniaturized Q-switched diode pumped solid state laser
    9.
    再颁专利
    Miniaturized Q-switched diode pumped solid state laser 失效

    公开(公告)号：USRE34192E

    公开(公告)日：1993-03-09

    申请号：US837098

    申请日：1992-02-18

    Applicant: Thomas M. Baer

    Inventor： Thomas M. Baer

    IPC: G02B6/42 ,  H01S3/02 ,  H01S3/11 ,  H01S3/117 ,  H01S3/16

    CPC classification number: G02B6/4204 ,  G02B6/4226 ,  H01S3/025 ,  H01S3/09415 ,  H01S3/117 ,  H01S3/1611 ,  H01S3/1643 ,  H01S3/1663

    Abstract: A miniaturized Q-switch is added to the resonant cavity of a compact laser diode pumped solid state laser to produce short high peak power pulses. Q-switching the compact diode pumped solid state lasers takes advantage of the relatively high gain and short cavity length to provide a desirable combination of pulsewidth and pulse energy. Nd:YAG or Nd:YLF are useful solid state laser materials for Q-switching, or other longer lifetime rare earth ions such as erbium or holmium for greater energy storage. The Q-switch is formed of a material such as TeO.sub.2, SF.sub.10, or LiNbO.sub.3 with an acoustooptic figure of merit substantially greater than fused silica. Pulsewidths of 10-50 ns are achieved at pulse energies of 10-20 microjoules. TEMOO output is easily produced by the compact laser pumped solid state resonator. Applications include materials processing, link blowing in semiconductor memories, marking and scribing, and optical time domain reflectometry.

    KTP crystal nonlinear optical device with reduced drift and damage
    10.
    发明授权
    KTP crystal nonlinear optical device with reduced drift and damage 失效
    KTP晶体非线性光学器件具有降低的漂移和损伤

    公开(公告)号：US4913533A

    公开(公告)日：1990-04-03

    申请号：US136896

    申请日：1987-12-22

    Applicant: James D. Kafka ,  Thomas M. Baer ,  Richard L. Herbst

    Inventor： James D. Kafka ,  Thomas M. Baer ,  Richard L. Herbst

    IPC: G02F1/35 ,  G02F1/37

    CPC classification number: G02F1/37 ,  G02F1/3525

    Abstract: A KTP crystal in a nonlinear optical device is heated to and operated at a temperature of at least 50.degree. C. and less than 350.degree. C., and more preferably about 90.degree. C. to about 200.degree. C., and most preferably to about 100.degree. C. to about 125.degree. C., to reduce drift and damage. The KTP crystal is placed in an oven or other heating device, which may be regulated or unregulated. The KTP may be cut at the room temperature phase matching angle and angle tuned for operation at the higher temperature, or the KTP may be cut at the correct angle for phase matching at the operating temperature.

    Abstract translation: 将非线性光学器件中的KTP晶体加热至至少50℃且小于350℃，更优选约90℃至约200℃的温度下操作，最优选至 约100℃至约125℃，以减少漂移和损坏。 将KTP晶体放置在烘箱或其他加热装置中，其可以调节或不调节。 可以在室温相位匹配角度和角度下切割KTP以在较高温度下操作，或者KTP可以在正确的角度切割以在工作温度下进行相位匹配。

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