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    Real-time PCR system
    1.
    发明授权
    Real-time PCR system 有权
    实时PCR系统

    公开(公告)号：US08017380B2

    公开(公告)日：2011-09-13

    申请号：US12105722

    申请日：2008-04-18

    申请人： Toshiki Moriwaki ,  Tasuku Yotoriyama ,  Yuji Segawa

    发明人： Toshiki Moriwaki ,  Tasuku Yotoriyama ,  Yuji Segawa

    IPC分类号： C12Q1/68 ,  C12M1/36 ,  C12M1/38 ,  C12M3/00 ,  C12M1/34

    CPC分类号： G01K7/015 ,  B01J2219/00783 ,  B01J2219/00853 ,  B01J2219/00873 ,  B01L7/52 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/1827 ,  G01N21/6428

    摘要： A real-time PCR system for detecting gene expression levels includes plural reaction regions, a like plural number of heating portions arranged corresponding to the reaction regions and having heat sources, respectively, an optical unit capable of irradiating exciting light of a specific wavelength to all of the plural reaction regions, and a like plural number of fluorescence detecting portions arranged corresponding to the reaction regions, respectively. The heating portions are each provided with a temperature detector for detecting a temperature in a vicinity of the corresponding heat source and converting the temperature into an electrical signal and also with a controller for controlling a thermal dose from the corresponding heat source based on a correlation between electrical signals and calorific values of the heat source stored beforehand.

    摘要翻译： 用于检测基因表达水平的实时PCR系统包括多个反应区域，分别对应于反应区域排列并具有热源的多个加热部分，能够将特定波长的激发光全部照射到所有的光学单元 的多个反应区域，以及分别对应于反应区域排列的多个荧光检测部分。 加热部分每个设有温度检测器，用于检测相应热源附近的温度并将温度转换成电信号，并且还具有控制器，用于根据相应的热源之间的相关性控制热剂量 预先存储的热源的电信号和发热量。

    REAL-TIME PCR SYSTEM
    2.
    发明申请
    REAL-TIME PCR SYSTEM 有权
    实时PCR系统

    公开(公告)号：US20080280350A1

    公开(公告)日：2008-11-13

    申请号：US12105722

    申请日：2008-04-18

    申请人： Toshiki Moriwaki ,  Tasuku Yotoriyama ,  Yuji Segawa

    发明人： Toshiki Moriwaki ,  Tasuku Yotoriyama ,  Yuji Segawa

    IPC分类号： C12M1/38

    CPC分类号： G01K7/015 ,  B01J2219/00783 ,  B01J2219/00853 ,  B01J2219/00873 ,  B01L7/52 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/1827 ,  G01N21/6428

    摘要： A real-time PCR system for detecting gene expression levels includes plural reaction regions, a like plural number of heating portions arranged corresponding to the reaction regions and having heat sources, respectively, an optical unit capable of irradiating exciting light of a specific wavelength to all of the plural reaction regions, and a like plural number of fluorescence detecting portions arranged corresponding to the reaction regions, respectively. The heating portions are each provided with a temperature detector for detecting a temperature in a vicinity of the corresponding heat source and converting the temperature into an electrical signal and also with a controller for controlling a thermal dose from the corresponding heat source based on a correlation between electrical signals and calorific values of the heat source stored beforehand.

    摘要翻译： 用于检测基因表达水平的实时PCR系统包括多个反应区域，分别对应于反应区域排列并具有热源的多个加热部分，能够将特定波长的激发光全部照射到所有的光学单元 的多个反应区域，以及分别对应于反应区域排列的多个荧光检测部分。 加热部分每个设有温度检测器，用于检测相应热源附近的温度并将温度转换成电信号，并且还具有控制器，用于根据相应的热源之间的相关性控制热剂量 预先存储的热源的电信号和发热量。

    Sample solution introduction kit and sample solution injector
    6.
    发明授权
    Sample solution introduction kit and sample solution injector 有权
    样品溶液介绍试剂盒和样品溶液注射器

    公开(公告)号：US08691163B2

    公开(公告)日：2014-04-08

    申请号：US13264291

    申请日：2010-04-19

    申请人： Tasuku Yotoriyama ,  Naohisa Sakamoto ,  Yuji Segawa ,  Satomi Tanaka

    发明人： Tasuku Yotoriyama ,  Naohisa Sakamoto ,  Yuji Segawa ,  Satomi Tanaka

    IPC分类号： B01J14/00

    CPC分类号： B01L3/502715 ,  B01L3/502723 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2200/142 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/049

    摘要： A sample solution introduction kit and a sample solution injector capable of introducing sample solution while reducing the rate of occurrence of an air bubble and having a simplified structure are proposed.A sample solution introduction kit includes a plate-like member and a sample solution injector. The plate-like member has a plurality of spaces formed therein and serving as reaction field and a communication space that communicates with the plurality of spaces therein and that has a portion defining an opening formed in a surface of the plate-like member, and the sample solution injector includes a container containing the sample solution, a tube that communicates with the bottom of the container and that is insertable into the opening, a stopper removably fitted into an opening formed at a top end of the tube, and liquid held in the container, and the liquid is insoluble in the sample solution and is lighter than the sample solution.

    摘要翻译： 提出了一种能够引入样品溶液同时降低气泡发生率并具有简化结构的样品溶液引入试剂盒和样品溶液注射器。 样品溶液引入试剂盒包括板状构件和样品溶液注入器。 板状构件具有形成在其中并用作反应场的多个空间和与其中的多个空间连通的连通空间，并且具有限定形成在板状构件的表面中的开口的部分， 样品溶液喷射器包括容纳样品溶液的容器，与容器的底部连通并且可插入到开口中的管，可拆卸地装配到形成在管的顶端的开口中的塞子，以及保持在 容器，液体不溶于样品溶液，比样品溶液轻。

    OPTICAL DETECTOR
    7.
    发明申请
    OPTICAL DETECTOR 有权
    光学检测器

    公开(公告)号：US20100291667A1

    公开(公告)日：2010-11-18

    申请号：US12775044

    申请日：2010-05-06

    申请人： Yuji Segawa ,  Tasuku Yotoriyama ,  Shinichi Kai

    发明人： Yuji Segawa ,  Tasuku Yotoriyama ,  Shinichi Kai

    IPC分类号： C12Q1/68

    CPC分类号： G01N21/6454 ,  B01L3/50851 ,  B01L7/52 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01N21/6428 ,  G01N2201/068

    摘要： Disclosed herein is an optical detector at least including: a first substrate in which a plurality of wells are formed; a second substrate in which a heating section is provided to heat the wells; a third substrate in which a plurality of photoirradiation sections are provided in alignment with the wells; and a fourth substrate in which a plurality of photodetection sections are provided in alignment with the wells.

    摘要翻译： 本文公开了一种光学检测器，至少包括：第一基板，其中形成有多个孔; 第二基板，其中设置加热部分以加热所述孔; 第三基板，其中设置有与所述孔对准的多个光照射部分; 以及第四基板，其中设置有与所述孔对准的多个光电检测部。

    SAMPLE SOLUTION INTRODUCTION KIT AND SAMPLE SOLUTION INJECTOR
    9.
    发明申请
    SAMPLE SOLUTION INTRODUCTION KIT AND SAMPLE SOLUTION INJECTOR 有权
    样品溶液介绍套件和样品溶液注射器

    公开(公告)号：US20120039774A1

    公开(公告)日：2012-02-16

    申请号：US13264291

    申请日：2010-04-19

    申请人： Tasuku Yotoriyama ,  Naohisa Sakamoto ,  Yuji Segawa ,  Satomi Tanaka

    发明人： Tasuku Yotoriyama ,  Naohisa Sakamoto ,  Yuji Segawa ,  Satomi Tanaka

    IPC分类号： B01J14/00

    CPC分类号： B01L3/502715 ,  B01L3/502723 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2200/142 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/049

    摘要： A sample solution introduction kit and a sample solution injector capable of introducing sample solution while reducing the rate of occurrence of an air bubble and having a simplified structure are proposed.A sample solution introduction kit includes a plate-like member and a sample solution injector. The plate-like member has a plurality of spaces formed therein and serving as reaction field and a communication space that communicates with the plurality of spaces therein and that has a portion defining an opening formed in a surface of the plate-like member, and the sample solution injector includes a container containing the sample solution, a tube that communicates with the bottom of the container and that is insertable into the opening, a stopper removably fitted into an opening formed at a top end of the tube, and liquid held in the container, and the liquid is insoluble in the sample solution and is lighter than the sample solution.

    摘要翻译： 提出了一种能够引入样品溶液同时降低气泡发生率并具有简化结构的样品溶液引入试剂盒和样品溶液注射器。 样品溶液引入试剂盒包括板状构件和样品溶液注入器。 板状构件具有形成在其中并用作反应场的多个空间和与其中的多个空间连通的连通空间，并且具有限定形成在板状构件的表面中的开口的部分， 样品溶液喷射器包括容纳样品溶液的容器，与容器的底部连通并且可插入到开口中的管，可拆卸地装配到形成在管的顶端的开口中的塞子，以及保持在 容器，液体不溶于样品溶液，比样品溶液轻。