公开(公告)号：US5521088A

公开(公告)日：1996-05-28

申请号：US77939

申请日：1993-06-18

申请人： Toshio Fujii ,  Akihiro Iwamatsu ,  Hiroyuki Yoshimoto ,  Toshitaka Minetoki ,  Takayuki Bogaki ,  Naoshi Nagasawa

发明人： Toshio Fujii ,  Akihiro Iwamatsu ,  Hiroyuki Yoshimoto ,  Toshitaka Minetoki ,  Takayuki Bogaki ,  Naoshi Nagasawa

IPC分类号： C12G1/02 ,  C12C11/00 ,  C12C11/02 ,  C12G1/022 ,  C12G3/02 ,  C12N1/19 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12R1/865 ,  C12N1/15 ,  C12N15/81

CPC分类号： C12C5/004 ,  C12C11/00 ,  C12C11/003 ,  C12C11/02 ,  C12C12/004 ,  C12C12/006 ,  C12C5/026 ,  C12G1/0203 ,  C12N9/1029

摘要： This invention disclosed herein provides an alcohol acetyl transferase ("AATase"), an AATase encoding gene and a yeast having an improved ester producing ability due to transformation with the AATase encoding gene. This invention also provides a process for producing an alcoholic beverage having an enriched ester flavor using the transformed yeast.

摘要翻译： 本发明公开了一种醇乙酰转移酶（“AATase”），AATase编码基因和由于用AATase编码基因转化而具有改进的酯生产能力的酵母。 本发明还提供了使用转化酵母生产具有富含酯味道的酒精饮料的方法。

公开(公告)号：US5658777A

公开(公告)日：1997-08-19

申请号：US461599

申请日：1995-06-05

申请人： Toshio Fujii ,  Akihiro Iwamatsu ,  Hiroyuki Yoshimoto ,  Toshitaka Minetoki ,  Takayuki Bogaki ,  Naoshi Nagasawa

发明人： Toshio Fujii ,  Akihiro Iwamatsu ,  Hiroyuki Yoshimoto ,  Toshitaka Minetoki ,  Takayuki Bogaki ,  Naoshi Nagasawa

IPC分类号： C12G1/02 ,  C12C11/00 ,  C12C11/02 ,  C12G1/022 ,  C12G3/02 ,  C12N1/19 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12R1/865 ,  C07H21/04

CPC分类号： C12C5/004 ,  C12C11/00 ,  C12C11/003 ,  C12C11/02 ,  C12C12/004 ,  C12C12/006 ,  C12C5/026 ,  C12G1/0203 ,  C12N9/1029

摘要： This invention disclosed herein provides an alcohol acetyl transferase ("AATase"), an AATase encoding gene and a yeast having an improved ester producing ability due to transformation with the AATase encoding gene. This invention also provides a process for producing an alcoholic beverage having an enriched ester flavor using the transformed yeast.

摘要翻译： 本发明公开了一种醇乙酰转移酶（“AATase”），AATase编码基因和由于用AATase编码基因转化而具有改进的酯生产能力的酵母。 本发明还提供了使用转化酵母生产具有富含酯味道的酒精饮料的方法。

公开(公告)号：US5728412A

公开(公告)日：1998-03-17

申请号：US465334

申请日：1995-06-05

申请人： Toshio Fujii ,  Akihiro Iwamatsu ,  Hiroyuki Yoshimoto ,  Toshitaka Minetoki ,  Takayuki Bogaki ,  Naoshi Nagasawa

发明人： Toshio Fujii ,  Akihiro Iwamatsu ,  Hiroyuki Yoshimoto ,  Toshitaka Minetoki ,  Takayuki Bogaki ,  Naoshi Nagasawa

IPC分类号： C12G1/02 ,  C12C11/00 ,  C12C11/02 ,  C12G1/022 ,  C12G3/02 ,  C12N1/19 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12R1/865 ,  A23L1/10 ,  A23L1/28 ,  C07H21/04 ,  C12N1/00 ,  C12N1/18 ,  C12N15/63

CPC分类号： C12C5/004 ,  C12C11/00 ,  C12C11/003 ,  C12C11/02 ,  C12C12/004 ,  C12C12/006 ,  C12C5/026 ,  C12G1/0203 ,  C12N9/1029

摘要： This invention disclosed herein provides an alcohol acetyl transferase ("AATase"), an AATase encoding gene and a yeast having an improved ester producing ability due to transformation with the AATase encoding gene, This invention also provides a process for producing an alcoholic beverage having an enriched ester flavor using the transformed yeast.

摘要翻译： 本发明公开了一种醇乙酰转移酶（“AATase”），AATase编码基因和由于用AATase编码基因转化而具有改进的酯生产能力的酵母。本发明还提供一种生产具有 使用转化的酵母富集酯味。

公开(公告)号：US5686284A

公开(公告)日：1997-11-11

申请号：US461621

申请日：1995-06-05

申请人： Toshio Fujii ,  Akihiro Iwamatsu ,  Hiroyuki Yoshimoto ,  Toshitaka Minetoki ,  Takayuki Bogaki ,  Naoshi Nagasawa

发明人： Toshio Fujii ,  Akihiro Iwamatsu ,  Hiroyuki Yoshimoto ,  Toshitaka Minetoki ,  Takayuki Bogaki ,  Naoshi Nagasawa

IPC分类号： C12G1/02 ,  C12C11/00 ,  C12C11/02 ,  C12G1/022 ,  C12G3/02 ,  C12N1/19 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12R1/865 ,  C07H21/04 ,  C12N9/00

CPC分类号： C12C5/004 ,  C12C11/00 ,  C12C11/003 ,  C12C11/02 ,  C12C12/004 ,  C12C12/006 ,  C12C5/026 ,  C12G1/0203 ,  C12N9/1029

摘要： This invention disclosed herein provides an alcohol acetyl transferase ("AATase"), an AATase encoding gene and a yeast having an improved ester producing ability due to transformation with the AATase encoding gene. This invention also provides a process for producing an alcoholic beverage having an enriched ester flavor using the transformed yeast.

公开(公告)号：US07135306B2

公开(公告)日：2006-11-14

申请号：US10546089

申请日：2004-01-28

申请人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

发明人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

IPC分类号： C12Q1/34 ,  C12Q1/37 ,  G01N33/53

CPC分类号： C12Q1/26 ,  C12N9/1007 ,  C12Q2326/00 ,  G01N33/6815

摘要： A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino acid acetyltransferase; (b) reducing homocysteine in the sample with a thiol compound; (c) reacting the reduced homocysteine with a methyltransferase and a methyl donor to newly produce D-amino acid; and (d) reacting the produced D-amino acid with the D-amino acid oxidase or the D-amino acid acetyltransferase in the presence of an SH reagent to produce hydrogen peroxide, and color-developing the produced hydrogen peroxide by using an oxidative color-developing agent.

摘要翻译： 用于检测或测量样品中同型半胱氨酸的方法包括以下步骤：（a）将样品中存在的D-氨基酸与D-氨基酸转化酶反应，以将D-氨基酸转化为不作为 D-氨基酸氧化酶或D-氨基酸乙酰转移酶的底物; （b）用硫醇化合物还原样品中的高半胱氨酸; （c）使还原的高半胱氨酸与甲基转移酶和甲基供体反应以新产生D-氨基酸; 和（d）在SH试剂的存在下使所产生的D-氨基酸与D-氨基酸氧化酶或D-氨基酸乙酰转移酶反应以产生过氧化氢，并且通过使用氧化颜色显色所产生的过氧化氢 发展代理

公开(公告)号：US20060166300A1

公开(公告)日：2006-07-27

申请号：US10546089

申请日：2004-01-28

申请人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

发明人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

IPC分类号： C12Q1/48

CPC分类号： C12Q1/26 ,  C12N9/1007 ,  C12Q2326/00 ,  G01N33/6815

摘要： A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino acid acetyltransferase; (b) reducing homocysteine in the sample with a thiol compound; (c) reacting the reduced homocysteine with a methyltransferase and a methyl donor to newly produce D-amino acid; and (d) reacting the produced D-amino acid with the D-amino acid oxidase or the D-amino acid acetyltransferase in the presence of an SH reagent to produce hydrogen peroxide, and color-developing the produced hydrogen peroxide by using an oxidative color-developing agent.