Reagent and process for the determination of the activity of the enzyme
gamma-glutamyl transferase
    2.
    发明授权
    Reagent and process for the determination of the activity of the enzyme gamma-glutamyl transferase 失效
    用于测定γ-谷氨酰转移酶酶活性的试剂和方法

    公开(公告)号:US4603107A

    公开(公告)日:1986-07-29

    申请号:US532907

    申请日:1983-09-16

    摘要: The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a colored compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity.The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents:.gamma.-GT-arylamide:1-100 mmol/l..gamma.-glutamyl acceptor (glycylglycine):10-500 mmol/l.coupling component:1-500 mmol/l.oxidation agent:0.1-500 mmol/l.buffer:10-1000 mmol/l.

    摘要翻译: 本发明提供了一种通过γ-谷氨酰基芳基酰胺与pH6至10的芳胺形成反应来动态测定γ-谷氨酰转移酶活性的方法,其中芳胺与芳族胺同时反应 或苯酚作为偶联组分和适当的氧化剂,得到最大吸收峰值高于565nm的着色化合物,其形成速率通过光度法监测并用作γ-谷氨酰转移酶活性的量度。 本发明还提供用于γ-GT活性的动力学测定的试剂,其中它含有以下试剂:γ-GT-芳基酰胺:1-100mmol / l。 γ-谷氨酰受体(甘氨酰甘氨酸):10-500mmol / l。 偶联组分:1-500 mmol / l。 氧化剂:0.1-500mmol / l。 缓冲液:10-1000mmol / l。

    Cuvette for the photo determination of chemical components in fluids
    10.
    发明授权
    Cuvette for the photo determination of chemical components in fluids 失效
    用于测定流体中化学成分的比色杯

    公开(公告)号:US4582684A

    公开(公告)日:1986-04-15

    申请号:US531439

    申请日:1983-09-12

    IPC分类号: G01N21/03 G01N31/22 G01N33/52

    摘要: A cuvette for the determination of a chemical component of a fluid by photo evaluation has two planar-shaped parts parallel to one another at least one of which is transparent in the spectral range used for the evaluation. A planar distance piece of a filamentary reticulation for receiving the fluid therein spaces the planar-shaped parts apart to set the length of the light path through the cuvette for the photo evaluation.

    摘要翻译: 用于通过光学评估测定流体的化学成分的比色皿具有彼此平行的两个平面形部分,其中至少一个在用于评估的光谱范围中是透明的。 用于接收流体的丝状网状物的平面距离片将平面形部分分开,以将通过比色杯的光路的长度设定为照相评估。