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公开(公告)号：US20130105393A1
公开(公告)日：2013-05-02
申请号：US13810157
申请日：2011-07-13
IPC分类号： G01N1/40
CPC分类号： G01N1/40 , G01N1/4005 , Y10T29/49
摘要： The present invention refers to a device, comprising a hollow body having at least one open end and at least one barrier inside or at the end of the hollow body, which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force like pressure, drag force or driving power to said barrier, the use of such a device for collection, storage, treatment or isolation of a biomolecule or biomolecules containing samples, a method for preparation of said device and a method for isolation or purification of any biomolecules using said device.
摘要翻译： 本发明涉及一种装置，其包括中空体，该中空体具有至少一个开口端和在中空体的内部或端部处的至少一个阻挡物，其在环境条件下对于液体和固体是不可渗透的，然而，变成液体 通过对所述屏障施加压力，牵引力或驱动力等外力，使用这种用于收集，储存，处理或分离生物分子或含有样品的生物分子的装置，所述装置的制备方法和 使用所述装置分离或纯化任何生物分子的方法。
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2.发明申请公开(公告)号：US20110244468A1
公开(公告)日：2011-10-06
申请号：US13132588
申请日：2009-12-04
申请人： Vera Holländer , Peter Porschewski
发明人： Vera Holländer , Peter Porschewski
IPC分类号： C12Q1/68 , G01N1/28 , G01N33/68 , G01N27/447 , C25B7/00
CPC分类号： C12N15/1003 , G01N33/5308 , G01N33/68
摘要： The present invention relates to a method of isolating/extracting in parallel various biomolecules, in particular nucleic acids and proteins, from the same fixed biological samples, to the quantification and analysis of the biomolecules isolated by the method of the invention, and to a kit for isolating/extracting in parallel various biomolecules from a fixed sample, to the use of said kit for diagnosing, prognosing, deciding the therapy of and monitoring the therapy of a disease.
摘要翻译： 本发明涉及将来自相同固定生物样品的各种生物分子，特别是核酸和蛋白质并行分离/提取到通过本发明方法分离的生物分子的定量和分析的方法，以及试剂盒 用于从固定样品中并行分离/提取各种生物分子，使用所述试剂盒来诊断，预测，决定治疗和监测疾病的治疗。
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公开(公告)号：US20100009349A1
公开(公告)日：2010-01-14
申请号：US12087167
申请日：2006-12-29
申请人： Vera Holländer
发明人： Vera Holländer
CPC分类号： B01L3/508 , B01L2300/046 , G01N1/30 , G01N1/31 , Y10T436/107497 , Y10T436/108331 , Y10T436/2525
摘要： The invention relates to a method for treating a biological sample, comprising the following method steps: i) preparation of a biological sample and ii) bringing the biological sample into contact with a composition, comprising: (1) 1 to 100 wt. % of at least one polyol and (2) 0 to 99 wt. % of at least one additive, wherein the total amount of components (1) and (2) is 100 wt. %. The invention further relates to biological samples obtained by said method, a method for analysis of a treated biological sample, devices for treating a biological sample, use of said devices, various kits and use of a composition.
摘要翻译： 本发明涉及一种用于处理生物样品的方法，包括以下方法步骤：i）制备生物样品，和ii）使生物样品与组合物接触，包括：（1）1至100重量％ ％的至少一种多元醇和（2）0〜99重量％ ％的至少一种添加剂，其中组分（1）和（2）的总量为100重量％。 ％。 本发明还涉及通过所述方法获得的生物样品，用于分析经处理的生物样品的方法，用于处理生物样品的装置，所述装置的使用，各种试剂盒和组合物的用途。
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公开(公告)号：US20100255524A1
公开(公告)日：2010-10-07
申请号：US12602142
申请日：2008-05-29
申请人： Vera Holländer
发明人： Vera Holländer
IPC分类号： C12Q1/02 , C07H21/00 , C07K14/00 , C12N5/071 , C12N5/04 , C12N1/16 , C12N7/00 , C12N1/20 , G01N33/48 , G01N33/92 , G01N33/50
CPC分类号： C12Q1/6806 , C12N15/1003 , Y10T436/142222 , C12Q2527/125
摘要： The present invention relates to a method of stabilizing a biological sample, having the steps of preparation of a biological sample, and of contacting the biological sample with a composition, having a substance according to the following structural formula: in which R1 is a hydrogen residue or a methyl residue, R2 and R3 are identical or different hydrocarbon residues with a length of the carbon chain of 1-20, and R4 is an oxygen, sulfur or selenium residue (FIG. 1).
摘要翻译： 本发明涉及一种稳定生物样品的方法，具有制备生物样品的步骤，以及将生物样品与具有根据以下结构式的物质的组合物接触：其中R1是氢残基 或甲基残基，R2和R3是碳链长度为1-20的相同或不同的烃残基，R4是氧，硫或硒残基（图1）。
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5.发明授权Compositions for the isolation and/or stabilization of nucleic acids in biological materials 有权生物材料中核酸分离和/或稳定化的组合物公开(公告)号：US07682790B2
公开(公告)日：2010-03-23
申请号：US11890415
申请日：2007-08-06
申请人： Vera Holländer , Ralf Wyrich , Uwe Oelmüller
发明人： Vera Holländer , Ralf Wyrich , Uwe Oelmüller
CPC分类号： C12N15/1003 , C07C211/62 , C07C211/63 , C07C211/64 , C07F9/54
摘要： The present invention relates to new compositions for isolating and/or stabilizing nucleic acids in materials of biological origin. The compositions comprise as an essential ingredient a cationic compound of general formula Y+R1R2R3R4X− wherein Y may represent nitrogen or phosphorus R1, R2, R3 and R4 independently of one another may represent a branched or unbranched C1-C20-alkyl group and/or a C6-C20-aryl group as well as a C6-C26-aralkyl group and X− may represent an anion of an inorganic or organic, mono- or polybasic acid.
摘要翻译： 本发明涉及用于在生物来源的材料中分离和/或稳定核酸的新组合物。 组合物包含通式Y + R1R2R3R4X-的阳离子化合物，其中Y可以表示氮或磷，R 1，R 2，R 3和R 4彼此独立地表示支链或非支链C 1 -C 20 - 烷基和/或 C 6 -C 20 - 芳基以及C 6 -C 26 - 芳烷基，X - 可以表示无机或有机的单或多元酸的阴离子。
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公开(公告)号：US20100021351A1
公开(公告)日：2010-01-28
申请号：US12518429
申请日：2007-12-12
申请人： Vera Holländer
发明人： Vera Holländer
IPC分类号： B01L3/00
CPC分类号： B01L3/502 , B01L3/5082 , B01L2200/0605 , B01L2300/046 , B01L2300/0672 , B01L2400/0683
摘要： The present invention relates to a container for receiving a liquid biological sample, with a volume for receiving the liquid biological sample, and with a filling and/or removal opening for filling the volume with the sample and for removing the sample from the volume. The invention is distinguished in that a constriction or partition forming an aperture is arranged in the volume in order to partition off a predefined measured volume from the volume.
摘要翻译： 本发明涉及一种用于接收液体生物样品的容器，其具有用于接收液体生物样品的体积，以及用于用样品填充体积的填充和/或去除开口，以及用于从体积中除去样品。 本发明的区别在于，在体积中布置形成孔的收缩部或分隔部，以便将预定义的测量体积与体积分隔开。
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公开(公告)号：US10273470B2
公开(公告)日：2019-04-30
申请号：US13580470
申请日：2011-02-25
摘要： The present invention pertains to a method for isolating RNA, including small RNA from a RNA and DNA containing sample, wherein the sample is lysed and the optionally further processed lysate is incubated with a DNase to degrade DNA prior to purifying the RNA from the optionally further processed lysate. It was found that performing the DNase digest prior to isolating the RNA from the lysate has considerable advantages.
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公开(公告)号：US10094749B2
公开(公告)日：2018-10-09
申请号：US13810157
申请日：2011-07-13
IPC分类号： G01N1/40
摘要： The present invention refers to a device, comprising a hollow body having at least one open end and at least one barrier inside or at the end of the hollow body, which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force like pressure, drag force or driving power to said barrier, the use of such a device for collection, storage, treatment or isolation of a biomolecule or biomolecules containing samples, a method for preparation of said device and a method for isolation or purification of any biomolecules using said device.
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9.发明申请公开(公告)号：US20130052721A1
公开(公告)日：2013-02-28
申请号：US13580470
申请日：2011-02-25
CPC分类号： C12N15/1003
摘要： The present invention pertains to a method for isolating RNA, including small RNA from a RNA and DNA containing sample, wherein the sample is lysed and the optionally further processed lysate is incubated with a DNase to degrade DNA prior to purifying the RNA from the optionally further processed lysate. It was found that performing the DNase digest prior to isolating the RNA from the lysate has considerable advantages.
摘要翻译： 本发明涉及一种从RNA和含有DNA的样品中分离RNA的方法，其中将样品裂解，并将任选进一步处理的裂解物与DNA酶一起温育以降解DNA，然后从任选进一步纯化RNA 处理的裂解物。 已经发现，在从裂解物分离RNA之前进行DNA酶消化物具有相当大的优点。
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10.发明授权Compositions for isolating and/or stabilising nucleic acids in biological material 有权用于在生物材料中分离和/或稳定核酸的组合物公开(公告)号：US07270953B2
公开(公告)日：2007-09-18
申请号：US10312745
申请日：2001-05-22
申请人： Vera Holländer , Ralf Wyrich , Uwe Oelmüller
发明人： Vera Holländer , Ralf Wyrich , Uwe Oelmüller
CPC分类号： C12N15/1003 , C07C211/62 , C07C211/63 , C07C211/64 , C07F9/54
摘要： The present invention relates to new compositions for isolating and/or stabilising nucleic acids in materials of biological origin. The compositions comprise as an essential ingredient a cationic compound of general formula Y+R1R2R3R4X− wherein Y may represent nitrogen or phosphorus R1, R2, R3 and R4 independently of one another may represent a branched or unbranched C1-C20-alkyl group and/or a C6-C20-aryl group as well as a C6-C26-aralkyl group and X− may represent an anion of an inorganic or organic, mono- or polybasic acid.
摘要翻译： 本发明涉及用于在生物来源的材料中分离和/或稳定核酸的新组合物。 组合物包含作为必要成分的通式为“-in-line-formula”的阳离子化合物=“In-line formula”end =“lead”→> Y < ？In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In 其中Y可以表示氮或磷R 1，R 2，R 3和R 3， 彼此独立地可以表示支链或非支链C 1 -C 20 - 烷基和/或C 6 - C 1 -C 20 - 芳基以及C 6 -C 26 - 芳烷基和X - O - >可以代表无机或有机单或多元酸的阴离子。
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