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    Characterization of the yeast transcriptome
    1.
    发明授权
    Characterization of the yeast transcriptome 有权
    表达酵母转录组

    公开(公告)号：US07504493B2

    公开(公告)日：2009-03-17

    申请号：US10915727

    申请日：2004-08-11

    申请人： Victor E. Velculescu ,  Bert Vogelstein ,  Kenneth Kinzler

    发明人： Victor E. Velculescu ,  Bert Vogelstein ,  Kenneth Kinzler

    IPC分类号： C12N15/11

    CPC分类号： C12Q1/6895 ,  C12Q1/6837 ,  C12Q2600/158

    摘要： Yeast genes which are differentially expressed during the cell cycle are described. They can be used to study, affect, and monitor the cell cycle of a eukaryotic cell. They can be used to obtain human homologs involved in cell cycle regulation. They can be used to identify antifungal agents and other classes of drugs. They can be formed into arrays on solid supports for interrogation of a cell's transcriptome under various conditions.

    摘要翻译： 描述在细胞周期期间差异表达的酵母基因。 它们可用于研究，影响和监测真核细胞的细胞周期。 它们可用于获得涉及细胞周期调节的人类同源物。 它们可用于鉴定抗真菌剂和其他类别的药物。 它们可以在固体支持物上形成阵列，用于在各种条件下询问细胞的转录组。

    Digital karyotyping
    2.
    发明授权
    Digital karyotyping 有权
    数字核型分析

    公开(公告)号：US07704687B2

    公开(公告)日：2010-04-27

    申请号：US10705874

    申请日：2003-11-13

    申请人： Tian-Li Wang ,  Victor Velculescu ,  Kenneth Kinzler ,  Bert Vogelstein

    发明人： Tian-Li Wang ,  Victor Velculescu ,  Kenneth Kinzler ,  Bert Vogelstein

    IPC分类号： C12Q1/68 ,  G06F19/00 ,  G01N33/48 ,  C40B40/08 ,  C12P19/34

    CPC分类号： C12Q1/6841 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2521/313

    摘要： Alterations in the genetic content of a cell underlie many human diseases, including cancers. A method called Digital Karyotyping provides quantitative analysis of DNA copy number at high resolution. This approach involves the isolation and enumeration of short sequence tags from specific genomic loci. Analysis of human cancer cells using this method identified gross chromosomal changes as well as amplifications and deletions, including regions not previously known to be altered. Foreign DNA sequences not present in the normal human genome could also be readily identified. Digital Karyotyping provides a broadly applicable means for systematic detection of DNA copy number changes on a genomic scale.

    摘要翻译： 细胞遗传物质的变化是许多人类疾病，包括癌症的基础。 称为数字核型分析的方法提供高分辨率DNA拷贝数的定量分析。 这种方法涉及从特定基因组位点分离和枚举短序列标签。 使用该方法分析人类癌细胞鉴定出总体染色体变化以及扩增和缺失，包括以前未知已被改变的区域。 不存在于正常人类基因组中的外源DNA序列也可以容易地鉴定。 数字核型分析提供了广泛适用的方法，用于系统检测基因组规模上的DNA拷贝数变化。

    Secreted and cytoplasmic tumor endothelial markers
    6.
    发明申请
    Secreted and cytoplasmic tumor endothelial markers 审中-公开
    分泌和细胞质肿瘤内皮标志物

    公开(公告)号：US20090233270A9

    公开(公告)日：2009-09-17

    申请号：US10519805

    申请日：2003-07-02

    申请人： Brad St. Croix ,  Kenneth Kinzler ,  Bert Vogelstein

    发明人： Brad St. Croix ,  Kenneth Kinzler ,  Bert Vogelstein

    IPC分类号： C12Q1/68 ,  G01N33/567 ,  A61K48/00 ,  A61K38/17

    CPC分类号： G01N33/56966 ,  G01N33/574

    摘要： To gain a better understanding of tumor angiogenesis, new techniques for isolating endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, over 170 genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed many differentially expressed genes, including a large nujber of genes that were specifically elevated in tumor-associated endothelium. Experiments with representative genes from this group demonstrated that most were similarly expressed in the endothelium of primary lung, breast, brain, and pancreatic cancers as well as in metastatic lesions fo the liver. Theses results demonstrate that neoplastic and normal endothelium in humans are distinct at the molecular level, and have significant implications for the development of anti-angiogenic.

    摘要翻译： 为了更好地了解肿瘤血管生成，开发了分离内皮细胞（ECs）和评估基因表达模式的新技术。 将来自正常和恶性结肠直肠组织的ECs的转录物与来自非内皮细胞的转录物进行比较，鉴定了主要在内皮中表达的170个以上的基因。 正常和肿瘤衍生的内皮的比较显示许多差异表达的基因，包括在肿瘤相关内皮中特异性升高的大量的基因。 来自该组的代表性基因的实验证明，大多数类似地在原发性肺，乳腺，脑和胰腺癌的内皮以及肝脏的转移性损伤中表达。 这些结果表明，人类的肿瘤和正常内皮在分子水平上是不同的，对抗血管生成的发展具有重要意义。

    Method and compositions for detection and enumeration of genetic variations
    7.
    发明申请
    Method and compositions for detection and enumeration of genetic variations 有权
    用于检测和计数遗传变异的方法和组合物

    公开(公告)号：US20070065823A1

    公开(公告)日：2007-03-22

    申请号：US10562840

    申请日：2004-06-09

    申请人： Devin Dressman ,  Hai Yan ,  Kenneth Kinzler ,  Bert Vogelstein

    发明人： Devin Dressman ,  Hai Yan ,  Kenneth Kinzler ,  Bert Vogelstein

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M3/00

    CPC分类号： C12Q1/6858 ,  C07H21/04 ,  C12N15/1075 ,  C12Q1/686 ,  C12Q2565/537 ,  C12Q2563/149 ,  C12Q2563/143

    摘要： Many areas of biomedical research depend on the analysis of uncommon variations in individual genes or transcripts. Here we describe a method that can quantify such variation at a scale and ease heretofore unattainable. Each DNA molecule in a collection of such molecules is converted into a single particle to which thousands of copies of DNA identical in sequence to the original are bound. This population of beads then corresponds to a one-to-one representation of the starting DNA molecules. Variation within the original population of DNA molecules can then be simply assessed by counting fluorescently-labeled particles via flow cytometry. Millions of individual DNA molecules can be assessed in this fashion with standard laboratory equipment. Moreover, specific variants can be isolated by flow sorting and employed for further experimentation. This approach can be used for the identification and quantification of rare mutations as well as to study variations in gene sequences or transcripts in specific populations or tissues.

    摘要翻译： 生物医学研究的许多领域取决于对个别基因或转录本中不常见变异的分析。 在这里，我们描述一种可以量化这种变化的方法，其规模和容易度迄今为止是无法实现的。 将这样的分子的集合中的每个DNA分子转化成单个颗粒，其中与原始序列顺序相同的数千个拷贝的DNA被结合。 这种珠子群对应于起始DNA分子的一对一表示。 然后可以通过流式细胞术对荧光标记的颗粒进行计数，简单地评估原始DNA分子群体内的变异。 可以用标准实验室设备以这种方式评估数百万个单独的DNA分子。 此外，可以通过流动分选分离特定的变体并用于进一步的实验。 该方法可用于鉴定和定量稀有突变，并研究特定群体或组织中基因序列或转录本的变异。

    Combination bacteriolytic therapy for the treatment of tumors
    10.
    发明申请
    Combination bacteriolytic therapy for the treatment of tumors 有权
    联合溶菌治疗肿瘤治疗

    公开(公告)号：US20050079157A1

    公开(公告)日：2005-04-14

    申请号：US10495116

    申请日：2002-11-21

    申请人： Long Dang ,  Kenneth Kinzler ,  Bert Vogelstein

    发明人： Long Dang ,  Kenneth Kinzler ,  Bert Vogelstein

    IPC分类号： A61K31/475 ,  A61K35/74 ,  A61K35/742 ,  A61K41/00 ,  A61K45/06 ,  C12N1/21 ,  A61K48/00

    CPC分类号： C12R1/145 ,  A61K31/475 ,  A61K35/742 ,  A61K38/193 ,  A61K41/00 ,  A61K45/06 ,  C12N1/36 ,  A61K2300/00

    摘要： Current chemotherapeutic approaches for cancer are in part limited by the inability of drugs to destroy neoplastic cells within poorly vascularized compartments of tumors. We have here systematically assessed anaerobic bacteria for their capacity to grow expansively within avascutar compartments of transplanted tumors. Among 26 different strains tested, one (Clostridium novyi) appeared particularly promising. We created a strain of C. novyi devoid of its lethal toxin (C. novyi-NT) and showed that intravenously injected C. novyi-NT spores germinated within the avascular regions of tumors in mice and destroyed surrounding viable tumor cells. When C. novyi-NT spores were administered together with conventional chemotherapeutic drugs, extensive hemorrhagic necrosis of tumors often developed within 24 hours, resulting in significant and prolonged anti-tumor effects. This strategy, called combination bacteriolytic therapy (COBALT), has the potential to add a valuablle dimension to the treatment of cancer.

    摘要翻译： 目前用于癌症的化学治疗方法在一定程度上受到药物不能破坏肿瘤不良血管化区域内的肿瘤细胞的作用。 我们在这里系统地评估了厌氧细菌在移植肿瘤的avascutar区域内扩大生长的能力。 在测试的26种不同菌株中，1种（Clostridium novyi）出现特别有前途。 我们创建了一种没有其致死毒素（C. novyi-NT）的C. novyi菌株，并且显示在小鼠的肿瘤的无血管区域内静脉内注射C.novyi-NT孢子，并破坏周围的活的肿瘤细胞。 当与常规化疗药物一起施用C.novyi-NT孢子时，广泛的肿瘤出血性坏死通常在24小时内发展，导致显着的和长期的抗肿瘤效应。 这种称为组合溶菌治疗（COBALT）的策略有可能为治疗癌症增加一个价值。